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Book The Effect of Nutritional Programming on Gut Microbiota in Broodstock and Progeny Fish

Download or read book The Effect of Nutritional Programming on Gut Microbiota in Broodstock and Progeny Fish written by Samuel Patula and published by . This book was released on 2020 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aquaculture is currently the fastest growing animal production sector. Because the aquaculture sector is growing at rapid rates, certain materials for feed, specifically marine protein sources, are becoming increasingly expensive and unsustainable. To counteract the reliance on fishmeal (FM) and other marine protein sources in the industry plant protein (PP), specifically soybean meal (SBM), has been investigated to replace FM as a protein source. Unfortunately, SBM when given in high quantities (greater than 30%) has been shown to negatively affect fish performance including retarded growth, intestinal inflammation, reduction of spawn quality, as well as dysbiosis in the gut microbiome, most likely due to presence of antinutritional factors such as saponins and tannins in SBM. The goal of this thesis was to investigate the effect of nutritional programming (NP) with SBM-based diet on gut microbiota in broodstock and progeny fish. Three feedings trials were conducted to test the efficacy of 3 approaches towards improving the use of PP in fish. The first trial (Chapter 2), tested the effect of NP on larval zebrafish (Danio rerio). NP is the theory of introducing an early nutritional stimulus to an animal that will 'program' the animal to better utilize the stimuli later in its adult life. The zebrafish were programmed in their larval stages, and the trial lasted for 65 days. There was a significant effect on growth performance for the programmed group (NP-PP) in terms of weight gains, as the NP-PP group grew better compared to the non-programmed group (NP-FM) and negative control (-control). There was no significant effect on the gut microbiome in terms of alpha or beta diversity, however, there were significant changes in the relative abundance (RA) of the gut microbiome throughout time in the NP-PP and the NP-FM groups. The findings of the study support that early NP of zebrafish with SBM improves growth performance on PP diet, but the gut microbiome does not seem to be a mechanism for NP. The second feeding trial (Chapter 3) focused on NP induced in the zebrafish broodstock with dietary SBM. For two weeks, the broodstock fish were fed with either a SBM diet or a FM diet so that gametogenesis occurred with either a FM or PP diet. This phase was called the 'broodstock programming' stage. The broodstock were then spawned, and the larval fish were separated into four different treatments: 1) SBM broodstock progeny, fed SBM for the entire trial (PPBS-PP) 2) SBM broodstock progeny fed FM the entire trial (PPBS-FM), 3) FM broodstock progeny fed FM the entire trial (+ control, FMBS-FM), and 4) FM broodstock progeny fed SBM the entire trial (- control, FMBS-PP). The PPBS-PP group achieved similar weight gains compared to all other treatments in terms of grams, but was numerically greater than the FMBS-PP treatment. There were no differences detected in gut microbiome alpha or beta diversity in any of the groups, however, there was significant change observed of certain bacterial phyla between the 'programmed broodstock', larval fish, and fish at the end of the trial, 48 days post hatch. Overall, this trial suggests that parental programming does not improve PP utilization in the progeny of zebrafish. It also appears that the gut microbiome is not a mechanism of parental programming. The third feeding trial (Chapter 4), was conducted on largemouth bass (Micropterus salmoides). This chapter had a similar experimental design as the first trial (Chapter 2), and larval largemouth bass were programmed with dietary SBM. This trial had an additional group added to it, which included a dietary saponin-programmed group. The study found that the NP with SBM diet or dietary saponin did not improve PP utilization and growth performance of largemouth bass in its pre-adult age. The study also found that the NP with SBM diet or dietary saponin did not have any effect on the largemouth bass gut microbiome, and there does not seem to be any gut microbiome modification associated with the NP in this fish species. Overall, NP can be used to improve dietary PP utilization but optimal timing and PP delivery method must be well assessed to ensure successful PP exposure and adaptation in different species. Nevertheless, the gut microbiome does not seem to be affected by NP and therefore is not considered the mechanism behind NP. Finally, studies on both zebrafish and largemouth bass presented major shifts in the gut microbiome as the fish aged. In addition, the core microbiomes of both species appeared to become more pronounced as the fish become adults. There seem to be an evolutionary tie between host and its gut microbiome. More studies, however, should further investigate this and the genetic effects on gut microbiota development and its heritability.

Book Aquaculture Nutrition

Download or read book Aquaculture Nutrition written by Daniel L. Merrifield and published by John Wiley & Sons. This book was released on 2014-08-13 with total page 519 pages. Available in PDF, EPUB and Kindle. Book excerpt: Manipulation of the microbial gut content of farmed fishes and crustaceans can have a marked effect on their general health, growth, and quality. Expertly covering the science behind the use of prebiotics and probiotics this landmark book explains how the correct manipulation of the gut flora of farmed fishes and crustaceans can have a positive effect on their health, growth rates, feed utilization, and general wellbeing. Aquaculture Nutrition: Gut Health, Probiotics and Prebiotics provides a comprehensive overview of the current knowledge of the gut microbiomes of fish and their importance with respect to host-fish health and performance, providing in-depth, cutting-edge fundamental and applied information. Written by many of the world’s leading authorities and edited by Dr Daniel Merrifield and Professor Einar Ringø, this important book discusses in detail the common mechanisms for modulating microbiomes, particularly at the gut level (e.g. probiotics, prebiotics and synbiotics). The book is a key resource for an understanding of the historical development of these products, their known mechanisms of action and their degree of efficacy as presently demonstrated in the literature. The fundamental material provided on the gut microbiota itself, and more broad aspects of microbe-live feed interactions, provide essential reading for researchers, academics and students in the areas of aquaculture nutrition, fish veterinary science, microbiology, aquaculture, fish biology and fisheries. Those involved in the development and formulation of aquaculture feeds and those with broader roles within the aquaculture industry will find a huge wealth of commercially-important information within the book’s covers. All libraries in universities and research establishments where biological sciences, nutrition and aquaculture are studied and taught, should have copies of this excellent book on their shelves.

Book The Use of Nutritional Programming and Dipeptide Supplementation as a Means of Mitigating the Negative Effects of Plant Protein and Improving the Growth of Fish Fed with Plant based Diets

Download or read book The Use of Nutritional Programming and Dipeptide Supplementation as a Means of Mitigating the Negative Effects of Plant Protein and Improving the Growth of Fish Fed with Plant based Diets written by Giovanni Molinari and published by . This book was released on 2020 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Currently aquaculture is the largest growing food sector in the world, however, its future growth is limited by its heavy reliance on fishmeal (FM). Overfishing of wild marine fish stocks used for FM is putting too much pressure on the stocks, making FM unsustainable as a dominant protein source as aquaculture continues to grow. Plant proteins (PP) are an ideal alternative to FM because of their wide availability and relatively low cost. Soybean meal (SBM) is the most commonly used PP in aquaculture, but its inclusion in feeds is limited due to reduced digestibility and the presence of antinutritional factors ultimately leading to a reduced growth performance of fish that are fed with the SBM-based diet. Therefore, the goal of this thesis was to mitigate the negative effects of PP and improve the growth of fish fed with a PP-based diet, utilizing SBM as our PP in feeds. Three feeding trials were conducted to test the efficacy of 3 approaches towards improving the use of PP in fish. The first trial (Chapter 2), tested the effect of nutritional programming (NP) on the utilization of SBM in zebrafish (Danio rerio). NP is the theory that an organism can be 'programmed' to better utilize a dietary component by being exposed to that component in its early life stages. This study also tested the effect of NP through the broodstock by exposing the broodstock to SBM for 4 weeks prior to breeding. In addition, a combination of both programming techniques was also tested. The results found that neither of the programming techniques had a significant effect on the growth performance of the zebrafish. Among the two groups from the programmed broodstock, the group that also received early stage NP had a significantly higher expression of PepT1, a di- and tri- peptide transporter. Also, the dual programmed group had the highest length-to-width ratio of the distal villi among groups that were fed SBM, which signifies an increase in surface area for nutrient absorption in the intestine. The findings from this study suggest that early stage NP may increase the absorption of nutrient from PP-based feeds within the intestine. The second feeding trial (Chapter 3), utilized the supplementation of health-promoting dipeptides to improve the use of SBM-based feeds for zebrafish. The three dipeptides used in this study were alanyl-glutamine, carnosine, and anserine. The five groups in this study consisted of three groups receiving an SBM-based diet with one of the dipeptides supplemented into it, a (-) Control group receiving a non-supplemented SBM-based diet, and a (+) Control group receiving a FM-based diet. Both the alanyl-glutamine and carnosine supplemented groups experienced a significantly higher weight gain compared to the (-) Control group. In addition, the alanyl-glutamine supplemented group had a significantly higher length-to-width ratio of the intestinal villi and, had a numerically higher expression of both nutrient absorption genes measured, PepT1 and fabp2, compared to the (-) Control group. This finding suggests that the supplementation of alanyl-glutamine into SBM-based diets may improve the intestinal absorptive capacity of the fish fed with the SBM. The results from this study also support the use of both alanyl-glutamine and carnosine supplementation as a means of improving the growth performance of fish fed with a SBM-based diet. The third feeding trial (Chapter 4), was conducted on largemouth bass (LMB) (Micropterus salmoides). This study also focused on NP as a means of improving the utilization of dietary SBM, similar to Chapter 2. In this study, live feed was used as a vector to program the larval LMB to SBM. The programmed group in this study received Artemia nauplii that were enriched with a SBM solution, during the larval stage. The bass were then fed with a FM-diet for 7 weeks, before being reintroduced to SBM for the final 6 weeks of the study (PP-Challenge). The programmed LMB experienced a significantly higher weight gain compared to the non-programmed fish also undergoing the PP-Challenge, and achieved a weight gain similar to that of the LMB that were being fed with an FM-diet. In addition, the programmed LMB had significantly longer distal villi and a higher length-to-width ratio of the villi, compared to the non-programmed group. The findings from this study support the use of live feed as a vector for NP and improving the growth performance of a carnivorous aquaculture species fed with a SBM-based diet. The overall findings from these studies suggest that both NP and dipeptide supplementation are feasible means of improving the utilization of SBM in fish. The mechanism behind NP seems to lie in the intestine. In both zebrafish and LMB, NP was found to reduce the inflammatory impact on the intestine and increase the surface area for absorption of the intestinal villi. The supplementation of alanyl-glutamine had similar effects on the intestine as NP and improved the growth performance of zebrafish. The observations from these studies seem to point to mitigating the negative effects of SBM on the intestine as the key to improving the growth performance of fish fed with a SBM-based diet.

Book Aquaculture

    Book Details:
  • Author :
  • Publisher : Academic Press
  • Release : 2020-12-01
  • ISBN : 0128226560
  • Pages : 386 pages

Download or read book Aquaculture written by and published by Academic Press. This book was released on 2020-12-01 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fish Physiology, Volume 38 in this ongoing series, examines how the inherent potential of fish to express traits of economic value can be realized through aquaculture. Topics covered include the regulation of the reproductive cycle of captive fish, shifting carnivorous fish towards plant-based diets, defining the challenges, opportunities and optimal conditions for growth under intensive culture (including in Recirculating Aquaculture Systems), enhancing immune function and fish health during culture, identifying and managing maladaptive physiological responses to aquaculture stressors, establishing welfare guidelines for farmed fish, phenotypic and physiological responses to genetic modification, Zebrafish as a research tool, and the aquaculture of air-breathing fish. Contains contributions from an international board of authors, each with decades of aquaculture expertise Provides the most up-to-date information on the fundamental role that physiology plays in optimizing fish performance in aquaculture Provides the latest release in the Fish Physiology series that tackles how the manipulation of biological processes can be used to maximize the expression of beneficial production traits in fish aquaculture

Book Fish behaviour and welfare

    Book Details:
  • Author : Pablo Almazan Rueda
  • Publisher : Frontiers Media SA
  • Release : 2024-09-19
  • ISBN : 283255430X
  • Pages : 136 pages

Download or read book Fish behaviour and welfare written by Pablo Almazan Rueda and published by Frontiers Media SA. This book was released on 2024-09-19 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fish welfare is currently one of the most important concerns in aquaculture, and knowledge on how to improve fish welfare should be practical and implementable for all aquaculture farms. The objective of this Research Topic is to extend the current knowledge on fish welfare and to identify more comprehensive and practical indicators of the existing standard for fish welfare that are easily, more comprehensive and practical indicators in order to make it easy to implement on fish farms. A secondary objective is to identify which welfare issues are most relevant. It is important to mention that good fish welfare cannot simply be extrapolated from terrestrial animals. It has to take into consideration that different species of fish must also be taken into consideration. Whether these are species of commercial interest or not, they have different behaviour and respond differently to stress, and consequently, require a species-specific approach to improving their welfare. Intensive fish production systems have important negative consequences for the animals including impaired health and erratic behaviour. For many industries, their main goal is to achieve higher production whilst majority of the time, welfare is not take it into consideration. Better conditions for intensively produced farm animals, including fish, should result in improved efficiency and performance and higher quality product for consumers. We welcome papers demonstrating such effects. This research topic is related to the welfare of farmed fish. Fish welfare is related to how we treat our fish: the type of feed, how often we feed them, the stocking density that it is used, the environment where they are growing, how we grow them, from fertilized egg to fish ready to sell for public consumption. This Research Topic should address fish welfare during the whole growth period, and may also include papers on the attitudes and perceptions of stakeholders, including fish farmers and public about fish welfare.

Book Soybean and Health

    Book Details:
  • Author : Hany El-Shemy
  • Publisher :
  • Release : 2011
  • ISBN : 9789535151715
  • Pages : 514 pages

Download or read book Soybean and Health written by Hany El-Shemy and published by . This book was released on 2011 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: Worldwide, soybean seed proteins represent a major source of amino acids for human and animal nutrition. Soybean seeds are an important and economical source of protein in the diet of many developed and developing countries. Soy is a complete protein, and soy-foods are rich in vitamins and minerals. Soybean protein provides all the essential amino acids in the amounts needed for human health. Recent research suggests that soy may also lower risk of prostate, colon and breast cancers as well as osteoporosis and other bone health problems, and alleviate hot flashes associated with menopause. This volume is expected to be useful for student, researchers and public who are interested in soybean.

Book Legumes Research

    Book Details:
  • Author : Jose C. Jimenez-Lopez
  • Publisher : BoD – Books on Demand
  • Release : 2022-10-12
  • ISBN : 180356914X
  • Pages : 374 pages

Download or read book Legumes Research written by Jose C. Jimenez-Lopez and published by BoD – Books on Demand. This book was released on 2022-10-12 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: Legumes have nutraceutical qualities that impart beneficial effects on human health. They are an alternative protein source with great potential for use in producing novel foods with improved nutritional properties. This book presents a comprehensive overview of legume proteins, including information on their nutritional and nutraceutical profiles, the health benefits of their compounds, and their underlying bioactivities such as anti-diabetic, hepatoprotective, anti-inflammatory, antioxidant, and anti-cancer properties.

Book A Hatchery Manual for the Common  Chinese  and Indian Major Carps

Download or read book A Hatchery Manual for the Common Chinese and Indian Major Carps written by V. G. Jhingran and published by WorldFish. This book was released on 1985 with total page 203 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book The Prokaryotes

    Book Details:
  • Author : Edward F. DeLong
  • Publisher : Springer
  • Release : 2013-03-22
  • ISBN : 9783642301421
  • Pages : 662 pages

Download or read book The Prokaryotes written by Edward F. DeLong and published by Springer. This book was released on 2013-03-22 with total page 662 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Prokaryotes is a comprehensive, multi-authored, peer reviewed reference work on Bacteria and Achaea. This fourth edition of The Prokaryotes is organized to cover all taxonomic diversity, using the family level to delineate chapters. Different from other resources, this new Springer product includes not only taxonomy, but also prokaryotic biology and technology of taxa in a broad context. Technological aspects highlight the usefulness of prokaryotes in processes and products, including biocontrol agents and as genetics tools. The content of the expanded fourth edition is divided into two parts: Part 1 contains review chapters dealing with the most important general concepts in molecular, applied and general prokaryote biology; Part 2 describes the known properties of specific taxonomic groups. Two completely new sections have been added to Part 1: bacterial communities and human bacteriology. The bacterial communities section reflects the growing realization that studies on pure cultures of bacteria have led to an incomplete picture of the microbial world for two fundamental reasons: the vast majority of bacteria in soil, water and associated with biological tissues are currently not culturable, and that an understanding of microbial ecology requires knowledge on how different bacterial species interact with each other in their natural environment. The new section on human microbiology deals with bacteria associated with healthy humans and bacterial pathogenesis. Each of the major human diseases caused by bacteria is reviewed, from identifying the pathogens by classical clinical and non-culturing techniques to the biochemical mechanisms of the disease process. The 4th edition of The Prokaryotes is the most complete resource on the biology of prokaryotes. The following volumes are published consecutively within the 4th Edition: Prokaryotic Biology and Symbiotic Associations Prokaryotic Communities and Ecophysiology Prokaryotic Physiology and Biochemistry Applied Bacteriology and Biotechnology Human Microbiology Actinobacteria Firmicutes Alphaproteobacteria and Betaproteobacteria Gammaproteobacteria Deltaproteobacteria and Epsilonproteobacteria Other Major Lineages of Bacteria and the Archaea

Book Fish Oil Replacement and Alternative Lipid Sources in Aquaculture Feeds

Download or read book Fish Oil Replacement and Alternative Lipid Sources in Aquaculture Feeds written by Giovanni M. Turchini and published by CRC Press. This book was released on 2010-07-19 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experts are predicting that demand for marine fish oil will soon outstrip supply, creating extreme urgency within the global aquafeed industry to find viable alternatives. Fish Oil Replacement and Alternative Lipid Sources in Aquaculture Feeds is the first comprehensive review of this multifaceted, complex issue. It also addresses the crucial quest

Book Sturgeons and Paddlefish of North America

Download or read book Sturgeons and Paddlefish of North America written by G.T.O LeBreton and published by Springer Science & Business Media. This book was released on 2006-01-20 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern North American sturgeons and paddlefish are the result of 100 million years of evolution. Once an integral part of aboriginal culture, their numbers were decimated by overfishing and habitat destruction during the past two centuries. This book details the extensive science aimed at helping these remarkable species recover from the brink of extinction, and describes the historical, biological, and ecological importance of North American sturgeon and paddlefish. The text is enhanced by photographs and detailed line drawings. This comprehensive volume will be an invaluable resource for researchers, educators, and consultants, in academic and government settings, who work to further scientific understanding of these fishes. No other single compilation has documented current information in such detail.

Book Biology of Stress in Fish

Download or read book Biology of Stress in Fish written by Carl B. Schreck and published by Academic Press. This book was released on 2016-11-01 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biology of Stress in Fish: Fish Physiology provides a general understanding on the topic of stress biology, including most of the recent advances in the field. The book starts with a general discussion of stress, providing answers to issues such as its definition, the nature of the physiological stress response, and the factors that affect the stress response. It also considers the biotic and abiotic factors that cause variation in the stress response, how the stress response is generated and controlled, its effect on physiological and organismic function and performance, and applied assessment of stress, animal welfare, and stress as related to model species. Provides the definitive reference on stress in fish as written by world-renowned experts in the field Includes the most recent advances and up-to-date thinking about the causes of stress in fish, their implications, and how to minimize the negative effects Considers the biotic and abiotic factors that cause variation in the stress response

Book Emerging Issues in Fish Larvae Research

Download or read book Emerging Issues in Fish Larvae Research written by Manuel Yúfera and published by Springer. This book was released on 2018-01-22 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with the fundamentals of key physiological mechanisms involved in the development and growth of fish larvae. Chapters included show how the environmental and nutritional conditions are affecting the developmental process from its molecular basis and how these same conditions also influence the final characteristics of late larvae and fry. This volume provides recent findings on the importance of environmental rhythms, some specific nutrients and the adequate microbial environment in the developmental processes including recent results of current research projects.

Book Polyunsaturated Fatty Acid Metabolism

Download or read book Polyunsaturated Fatty Acid Metabolism written by Graham C. Burdge and published by Elsevier. This book was released on 2018-05-04 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polyunsaturated Fatty Acid Metabolism explores a number of major roles of PUFA in the body, including its role as a component of cell membranes and how it provides substrates for the synthesis of lipid second messengers. Recent studies are unraveling the effect of interactions between diet and endocrine factors and genetic and epigenetic variation on the regulation of PUFA biosynthesis in animals. Together, these recent findings provide novel insights into the impact of differences in PUFA supply on health. This book captures these findings in a manner that marks the state-of-the-art, placing them in the wider context of PUFA metabolism and nutritional science. Users will find a comprehensive discussion on the topic that presents the contributions of leading researchers who combine their knowledge to create a cohesive academic resource for researchers, those involved in production, and health policymakers. - Provides a comprehensive view of polyunsaturated fatty acid metabolism - Describes underlying metabolism on lipids that include polyunsaturated fatty acids - Includes discussions on recent findings on the genetic and epigenetic regulation of polyunsaturated fatty acid metabolism

Book Channel Catfish Farming Handbook

Download or read book Channel Catfish Farming Handbook written by Craig C. Tucker and published by Springer Science & Business Media. This book was released on 1990-12-31 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Freshwater Prawn Culture

Download or read book Freshwater Prawn Culture written by Michael Bernard New and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: The farming of the freshwater prawn Macrobrachium rosenbergii has developed rapidly during recent years. Advances in techniques, and the huge expansion of world demand for this species, continue to stimulate the growth of a multi-million dollar industry. This landmark publication is a compendium of information on every aspect of the farming of M. rosenbergii. A comprehensive review of the status of freshwater prawn farming research, development and commercial practice, the book is intended to stimulate further advances in the knowledge and understanding of this important field. An extremely well-known and internationally-respected team of contributing authors have written cutting edge chapters covering all major aspects of the subject. Coverage includes biology, hatchery and grow-out culture systems, feeds and feeding, up-to-date information on the status of freshwater prawn farming around the world, post-harvest handling and processing, markets, and economics and business management. Further chapters are devoted to the culture of other prawn species, prawn capture fisheries and the sustainability of freshwater prawn culture. Contributions to the book have been brought together and edited by Michael New and Wagner Valenti, themselves widely known for their work in this area. The comprehensive information in Freshwater Prawn Culture will give an important commercial edge to anyone involved in the culture and trade of freshwater prawns. Readership should include prawn farm personnel, business managers and researchers, and invertebrate, freshwater and crustacean biologists. Copies of the book should be available on the shelves of all libraries in research establishments and universities where aquaculture and fisheries are studied and taught. Michael Bernard New, OBE is a Past-President of the World Aquaculture Society and President-Elect of the European Aquaculture Society; Wagner Cotroni Valenti is a Professor at the Aquaculture Center, São Paulo State University, Brazil.

Book The Zebrafish in Biomedical Research

Download or read book The Zebrafish in Biomedical Research written by Samuel Cartner and published by Academic Press. This book was released on 2019-11-22 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Zebrafish in Biomedical Research: Biology, Husbandry, Diseases, and Research Applications is a comprehensive work that fulfills a critical need for a thorough compilation of information on this species. The text provides significant updates for working vivarium professionals maintaining zebrafish colonies, veterinarians responsible for their care and well-being, zoologists and ethologists studying the species, and investigators using the species to gain critical insights into human physiology and disease. As the zebrafish has become an important model organism for the study of vertebrate development and disease, organ function, behavior, toxicology, cancer, and drug discovery, this book presents an important resource for future research. - Presents a complete view of the zebrafish, covering their biology, husbandry, diseases and research applications - Includes the work of world-renowned authors - Provides the first authoritative and comprehensive treatment of zebrafish in biomedical research as part of the ACLAM series