Download or read book The C Elegans Sex determining GLI Protein TRA 1A is Regulated by Sex specific Proteolysis microform written by Mara Schvarzstein and published by Library and Archives Canada = Bibliothèque et Archives Canada. This book was released on 2005 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: The C. elegans sex determination hierarchy specifies either one of two sexes: the hermaphrodite or the male. The tra-1 gene is the global terminal regulator of the somatic sex determination pathway. Active TRA-1 promotes female fates in the hermaphrodite by repressing the transcription of genes that direct male development. The objective of the work presented here is to understand how the activity of TRA-1 is regulated. The tra-1 gene encodes two transcripts, tra-1a and tra-1b. Males and hermaphrodites have similar levels of tra-1 transcripts, suggesting that tra-1 is regulated post transcriptionally. Using a new antibody against TRA-1 in whole mount immunostaining I observed that TRA-1 appears to be much more abundant in hermaphrodites than in males. Western blot analysis revealed similar levels of TRA-1A and TRA-1B in lysates from males and hermaphrodites. Additional TRA-1-specific species (90-110 kDa) were present in the hermaphrodite lysates but absent from male lysates. These isoforms are the most abundant form of TRA-1 protein in hermaphrodites. The hermaphrodite-specific TRA-1 isoforms appear to be phosphoisoforms of a single TRA-1 band. Here I also describe the identification of proteins that specifically interact with FEM-2 in affinity chromoatography experiments. Loss-of-function mutants and RNAi silencing of three of these genes appear to partially phenocopy fem-2 mutants. I show evidence that the hermaphrodite-specific isoform is generated by proteolytic processing of TRA-1A. Since the truncated TRA-1A isoforms are present in embryos before sexual differentiation is apparent, they are unlikely to be a result of female differentiation. Therefore I propose that these isoforms are responsible for the feminizing activity of the tra-1 gene. The truncation mapped to the region between amino acids 842 and 921. The observations that both a nonsense mutation that results in the production of a C-terminally truncated TRA-1A protein and truncated TRA-1A transgenes are feminizing, support my hypothesis that the truncated TRA-1 isoforms are responsible for the feminizing activity of the tra-1 gene. Genetic analyses indicate that the fem gene products regulate, directly or indirectly, the activity of TRA-1. Therefore, characterization of these putative FEM-2 binding proteins may identify the mechanism by which the FEMs regulate TRA-1 activity.
Download or read book Transcriptional and Post transcriptional Regulation of Xol 1 by the Primary Sex determination Singal in C Elegans written by Sharmon Monique Nicoll and published by . This book was released on 1998 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Interactions Between Two Caenorhabditis Elegans Sex Determining Proteins HER 1 and TRA 2A written by Roxana Sultan and published by . This book was released on 2000 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The free-living soil nematode 'Caenorhabditis elegans' exists in nature as one of two possible sexes, the male or the self-fertile hermaphrodite. Most genes controlling sex determination in 'C. elegans ' have been cloned and sequenced. These genes have been ordered into a signal transduction cascade based on their epistatic interactions. I have uncovered evidence supporting a direct interaction between the proteins encoded by two of the genes in the pathway, 'her-1' and 'tra-2'. This was achieved through binding assays involving immunofluorescent and immunoblotting techniques. The proposed interaction sheds light on the possible mechanisms by which HER-1 negatively regulates the activity of TRA-2A in male development.
Download or read book Identification of New Regulators of Sex specific Neurogenesis in C Elegans written by Jennifer Marie Ross Wolff and published by . This book was released on 2005 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Sexual Dimorphism in Caenorhabditis Elegans written by Kara Renee Thoemke and published by . This book was released on 2004 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The C Elegans Sex Determination Protein MOG 3 Functions in Meiosis and Binds to the CSL Co repressor CIR 1 written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In the germ line of the Caenorhabditis elegans hermaphrodite, nuclei either proliferate through mitosis or initiate meiosis, finally differentiating as spermatids or oocytes. The production of oocytes requires repression of the fem-3 mRNA by cytoplasmic FBF and nuclear MOG proteins. Here we report the identification of the sex determining gene mog-3 and show that in addition to its role in gamete sex determination, it is necessary for meiosis by acting downstream of GLP-1/Notch. Furthermore, we found that MOG-3 binds both to the nuclear proteins MEP-1 and CIR-1. MEP-1 is necessary for oocyte production and somatic differentiation, while the mammalian CIR-1 homolog counters Notch signaling. We propose that MOG-3, MEP-1 and CIR-1 associate in a nuclear complex which regulates different aspects of germ cell development. While FBF triggers the sperm/oocyte switch by directly repressing the fem-3 mRNA in the cytoplasm, the MOG proteins play a more indirect role in the nucleus, perhaps by acting as epigenetic regulators or by controlling precise splicing events.
Download or read book Evolutionary Biology Concepts Biodiversity Macroevolution and Genome Evolution written by Pierre Pontarotti and published by Springer Science & Business Media. This book was released on 2011-07-20 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: The annual Evolutionary Biology Meetings in Marseilles serve to gather leading scientists, promote the exchange of ideas and encourage the formation of international collaborations. This book contains the most essential contributions presented at the 14th Evolutionary Biology Meeting, which took place in September 2010. It comprises 19 chapters organized according to the following categories: · Evolutionary Biology Concepts · Biodiversity and Evolution · Macroevolution · Genome Evolution Offering an up-to-date overview of recent results in the field of evolutionary biology, this book is an invaluable source of information for scientists, teachers and advanced students.
Download or read book Fetal Therapy written by Mark D. Kilby and published by Cambridge University Press. This book was released on 2013 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the latest insights any fetal specialist needs and provides essential knowledge for professionals caring for women with high-risk pregnancies.
Download or read book Transgenic Models in Pharmacology written by Lutz Hein and published by Springer Science & Business Media. This book was released on 2003-10-10 with total page 692 pages. Available in PDF, EPUB and Kindle. Book excerpt: Up-to-date information on animal models generated by transgenic or gene targeting techniques. Naturally, the focus is on the mouse system. Each chapter has been written by leading experts in the field and gives an overview on existing animal models. This is facilitated by tables, which list the most important genetically engineered animal models and their phenotypes. This book aims at illustrating the impact of transgenic animal models in the field of Experimental Pharmacology and Toxicology, which includes their role in the understanding of basic cellular mechanisms, the evaluation of potential drug targets or the testing for drug effects.
Download or read book Hirschsprung s Disease and Allied Disorders written by Prem Puri and published by Springer. This book was released on 2019-05-23 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third edition of a comprehensive study of the neuronal disorders of the lower gastrointestinal tract in children. Important new studies and progress in research on bowel motility and motility disorders are covered in detail as well as new aspects concerning the embryology, functional anatomy of the enteric nervous system. In particular new insights have been received from the studies in the genetics of Hirschsprung's disease. Special emphasis is laid on new surgical techniques, especially on the laparoscopic approach in combination with Soave's or Duhamel's technique.
Download or read book Fortschritte der Chemie Organischer Naturstoffe Progress in the Chemistry of Organic Natural Products Progr s dans la Chimie des Substances Organiques Naturelles written by and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dorland s Dictionary of Medical Acronyms and Abbreviations written by Dorland and published by Elsevier Health Sciences. This book was released on 2015-07-24 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medical acronyms and abbreviations offer convenience, but those countless shortcuts can often be confusing. Now a part of the popular Dorland's suite of products, this reference features thousands of terms from across various medical specialties. Its alphabetical arrangement makes for quick reference, and expanded coverage of symbols ensures they are easier to find. Effective communication plays an important role in all medical settings, so turn to this trusted volume for nearly any medical abbreviation you might encounter. - Symbols section makes it easier to locate unusual or seldom-used symbols. - Convenient alphabetical format allows you to find the entry you need more intuitively. - More than 90,000 entries and definitions. - Many new and updated entries including terminology in expanding specialties, such as Nursing; Physical, Occupational, and Speech Therapies; Transcription and Coding; Computer and Technical Fields. - New section on abbreviations to avoid, including Joint Commission abbreviations that are not to be used. - Incorporates updates suggested by the Institute for Safe Medication Practices (ISMP).
Download or read book Dictionary of Medical Acronyms and Abbreviations written by Stanley Jablonski and published by Hanley & Belfus. This book was released on 2004-09 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dictionary lists acronyms and abbreviations occurring with a reasonable frequency in the literature of medicine and the health care professions. Abbreviations and acronyms are given in capital letters, with no punctuation, and with concise definitions. The beginning sections also include symbols, genetic symbols, and the Greek alphabet and symbols.
Download or read book The Plant Endoplasmic Reticulum written by David G. Robinson and published by Springer Science & Business Media. This book was released on 2006-08-15 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: The endoplasmic reticulum (ER), called "the mother of all membranes," is spotlighted in this timely new book. The work presented here is especially exciting since GFP-technology has provided new ways of looking at the dynamics of the ER and its relationship to other organelles, particularly the Golgi apparatus and peroxisomes. This book provides in-depth knowledge of the ER and the diverse roles it plays.
Download or read book Springer Handbook of Enzymes written by Dietmar Schomburg and published by . This book was released on 2001 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt: Each entry includes nomenclature, source organism, reaction and specificity, enzyme structure, isolation / preparation / mutation / application, stability, and literature references for each enzyme.
Download or read book Cell Polarity in Development and Disease written by Douglas W Houston and published by Academic Press. This book was released on 2017-11-14 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Polarity in Development and Disease offers insights into the basic molecular mechanisms of common diseases that arise as a result of a loss of ordered organization and intrinsic polarity. Included are diseases affecting highly polarized epithelial tissues in the lung and kidney, as well as loss and gain of cell polarity in the onset and progression of cancer. This book provides a basic resource for understanding the biology of polarity, offering a starting point for those thinking of targeting cell polarity for translational medical research. - Provides basic science understanding of cell polarity disease and development - Covers diseases affecting polarized epithelial tissues in the lung and kidney, also covering the progression of cancer - Includes historical context of cell polarity research for potential future breakthroughs
Download or read book Prenatal and Preimplantation Diagnosis written by Joann Paley Galst and published by Springer. This book was released on 2015-08-26 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to expand the awareness and understanding of the emotional sequelae of prenatal/preimplantation diagnosis, prenatal decision-making, pregnancy interruption for fetal anomaly, multifetal reduction for high-order multifetal pregnancies and preimplantation choices involving the selection of embryos. Featuring a multi-disciplinary approach, it examines prenatal and preimplantation diagnosis from medical, legal, ethical and psychosocial perspectives. Prenatal and Preimplantation Diagnosis is an excellent resource for obstetricians, reproductive endocrinologists, clinical geneticists, genetic counselors and mental health professionals seeking to better support patients faced with difficult choices.
