Download or read book The BAM Complex written by Susan Buchanan and published by Humana. This book was released on 2015-10-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.
Download or read book Structural Studies of the BAM Complex OmpU Outer Membrane Protein and Lipoprotein N acyl Transferase in Gram negative Bacteria written by Huanyu Li and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Impact of Conformational Dynamics on the Function of the BAM Complex written by Samuel Francis Haysom and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Membrane Biogenesis written by Jos A.F. Op den Kamp and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many individual aspects of the dynamics and assembly of biological membranes have been studied in great detail. Cell biological approaches, advanced genetics, biophysics and biochemistry have greatly contributed to an increase in our knowledge in this field.lt is obvious however, that the three major membrane constituents - lipids, proteins and carbohydrates- are studied, in most cases separately and that a coherent overview of the various aspects of membrane biogenesis is not readily available. The NATO Advanced Study Institute on "New Perspectives in the Dynamics of Assembly of Biomembranes" intended to provide such an overview: it was set up to teach students and specialists the achievements obtained in the various research areas and to try and integrate the numerous aspects of membrane assembly into a coherent framework. The articles in here reflect this. Statting with detailed contributions on phospholipid structure, dynamics, organization and biogenesis, an up to date overview of the basic, lipidic backbone of biomembranes is given. Extensive progress is made in the research on membrane protein biosynthesis. In particular the post- and co-translational modification processes of proteins, the mechanisms of protein translocation and the sorting mechanisms which are necessary to direct proteins to their final, intra - or extracellular destination have been characterized in detail. Modern genetic approaches were indispensable in this research area: gene cloning, hybrid protein construction, site directed mutagenesis and sequencing techniques elucidated many functional aspects of specific nucleic acid and amino acid sequences.
Download or read book BAM and Then It Hit Me written by Karen Brooks Hopkins and published by Simon and Schuster. This book was released on 2021-11-02 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: President Emerita of the Brooklyn Academy of Music (BAM) Karen Brooks Hopkins pens BAM…and Then It Hit Me, an inspiring memoir of her 36 years at the iconic cultural institution, America's oldest performing arts center. The book has a sharp focus on concepts such as leadership, innovation, urban revitalization (including the transformation of Brooklyn from Manhattan Outpost to the coolest neighborhood on the planet), as highly successful cultural fundraising played critical roles in the colorful evolution of this world-class cultural juggernaut in the performing arts.
Download or read book Spatiotemporal Topography of the BAM Complex in Relation to Peptidoglycan and Cell Division written by Elisa Consoli and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Paleo Takeout written by Russ Crandall and published by Victory Belt Publishing. This book was released on 2015-06-23 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: Even though we know full well that most restaurant foods are made using ingredients laden with chemicals and additives, most of us can’t seem to shake the desire for even just a taste. Not to mention that nothing is easier than picking up takeout, hitting the drive-thru, or ordering delivery—but at what cost? Paleo Takeout: Restaurant Favorites Without the Junk delivers much healthier but equally satisfying alternatives, offering delectable recipes that mimic the flavors of our drive-thru and delivery favorites—Paleo style! Russ Crandall teaches you step-by-step how to prepare meals in less than an hour—leaving no sacrifice of taste or time. Our modern lives are hectic: We all face the challenge of creating meals at home that are as quick and flavorful as those from our neighborhood takeout restaurants. It’s hard to beat the convenience of restaurant food, even when we know full well that it’s seldom a healthy choice. In Paleo Takeout: Restaurant Favorites Without the Junk, celebrated author Russ Crandall re-creates everyone’s favorite takeout meals, made in record time using wholesome ingredients, giving you all of the gratification and none of the regret! Inspired by beloved restaurant experiences, Paleo Takeout features more than 200 recipes expertly culled from Chinese, Korean, Japanese, Thai, Vietnamese, Indian, Italian, Mexican, Greek, and American cuisines. Inside, you’ll find everything from Chow Mein to Moo Shu Pork, and Thai Red Curry to Buffalo Wings, all with a focus of “fridge to face” in less than an hour. Also featured is an indispensible meal-planning guide to help you put everything together for a doable, lasting approach to cooking and health. Paleo Takeout: Restaurant Favorites Without the Junk proves that eating right in a way that satisfies even the choosiest of healthy eaters is not only possible but also a lot of fun
Download or read book Beartown written by Fredrik Backman and published by Simon and Schuster. This book was released on 2021-03-23 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: A poignant story of a hockey town paralyzed by scandal. Jobs are disappearing and Beartown is slowly dying, so for its citizens, hockey is everything. This is the story not just of hockey, but of a 15-year-old named Maya Andersson, whose father, Peter, the general manager of the hockey club, loves hockey, but loves his family more. Seventeen-year-old Kevin Erdahl is the star of Beartown, with a chance to go professional. One night, after a huge win, Maya goes to a raucous party at Kevin's house and is thrilled at his attention, but things get out of hand, and what takes place changes Beartown forever.
Download or read book Bacteriological Analytical Manual written by United States. Food and Drug Administration. Division of Microbiology and published by . This book was released on 1969 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Protein protein Interactions Involved in the Periplasmic Components of the beta barrel Assembly Machinery BAM Complex of Escherichia Coli written by Suraaj Kaur Aulakh and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The [beta]-barrel assembly machinery (BAM) complex plays the essential role of folding and inserting outer membrane proteins (OMPs) into the outer membrane of Gram-negative bacteria. In Escherichia coli, the BAM complex is comprised of five proteins: BamA, BamB, BamC, BamD, and BamE. This thesis project investigates the interactions between the periplasmic components of the BAM complex by analyzing complex formation using gel-filtration chromatography. Results from the interaction studies have identified the unstructured N-termini of BamC and BamE as requirements for BamCDE subcomplex formation. Furthermore, BamAPOTRA was shown to form stable BamAPOTRA-BamB and BamAPOTRA-BamD-BamE complexes, but was unable to form the latter complex in the presence of BamC. Together these results provide a model for how the proteins assemble and suggest that the complex is dynamic. By understanding how the BAM components come together brings us one step closer to determining their individual roles and how the BAM complex may function overall.
Download or read book The Structure of the beta barrel Assembly Machinery Complex written by and published by . This book was released on 2016 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt: [Beta]-Barrel outer membrane proteins (OMPs) are found in the outer membranes of Gram-negative bacteria and are essential for nutrient import, signaling, and adhesion. A 200-kilodalton five-component complex called the [beta]-barrel assembly machinery (BAM) complex has been implicated in the biogenesis of OMPs. In this paper, we report the structure of the BAM complex from Escherichia coli, revealing that binding of BamCDE modulates the conformation of BamA, the central component, which may serve to regulate the BAM complex. The periplasmic domain of BamA was in a closed state that prevents access to the barrel lumen, which indicates substrate OMPs may not be threaded through the barrel during biogenesis. Finally and further, conformational shifts in the barrel domain lead to opening of the exit pore and rearrangement at the lateral gate.
Download or read book Omp85 Family Proteins and the BAM Complex in Caulobacter Crescentus written by Khatira Anwari and published by . This book was released on 2011 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: The outer membrane of Gram-negative bacteria is a compartment that houses many proteins involved in basic physiological functions, virulence and multi-drug resistance, and is therefore important for cell survival. Most proteins in the outer membrane adopt a [beta]-barrel conformation, and require the [beta]-barrel assembly machinery (BAM) for integration into the outer membrane. The BAM complex is a multi-subunit protein complex present in the outer membrane of all Gram-negative bacteria. It comprises of a core [beta]-barrel protein, BamA and associated lipoproteins that collectively participate in the folding and insertion of [beta]-barrel proteins. BamA has functional homologues in eukaryotes that form the SAM complex in mitochondria for insertion and assembly of [beta]-barrel proteins into the mitochondrial outer membranes. Previous work by other groups in the field has used N. meningitidis ([beta]-proteobacteria) and E. coli (y-proteobacteria) to study the function of BamA and the BAM complex. In this comparative study, we will use Caulobacter crescentus as a model [alpha]-proteobacterium to learn about differences and similarities in the BAM complexes across the three different classes of proteobacteria. The combined use of bioinformatics and biochemical experiments in C. crescentus have shown some key differences with the absence of the BamC partner lipoprotein and the presence of a novel OmpA-like protein as well as two other uncharacterized lipoprotein partners for BamA. In Chapter 2, we perform a comparative analysis of BamA and the BamA-like protein (Omp68) in C. crescentus. The Omp85 superfamily contains BamA, Omp68 and TpsB proteins and using bioinformatics analysis, we proposed that Omp68 is an evolutionary intermediate of BamA and TpsB proteins. We first established a method for preparing outer membranes from C. crescentus and demonstrated that like BamA, Omp68 forms an oligomeric complex in the outer membrane although it has characteristics that could classify it as a TpsB protein. The unusual nature of Omp68 is discussed and its possible functions. A method for effective purification of the BAM complex from outer membranes was also established for identification and analysis of the different components of the complex.In Chapter 3, we demonstrate that the BAM complex possesses modular characteristics and contains BamA and six outer membrane lipoproteins in the [alpha]-proteobacterium, C. crescentus. In addition to the three known lipoproteins (BamB, BamD and BamE), we identify three other subunits (Pal, BamF and BamG) of which only Pal is essential. We propose that Pal is a protein that anchors the BAM complex to the peptidoglycan layer and promotes proximity to the inner membrane Sec machinery for efficient outer membrane protein assembly. We also show BamF is a genuine component of the BAM complex and a potential homologue of the BamC protein found in all other proteobacteria. Both BamF and BamC contain a conserved motif that is possibly important for docking onto the BAM complex.In Chapter 4, we focus on biochemical and structural characterisations of BamD from C. crescentus. We show BamD is an outer membrane lipoprotein that forms the halo module of the BAM complex. We also demonstrate BamD contains TPR motifs that are essential for functioning of the BAM complex and that protrude into the periplasm for protein-protein interactions. Further experiments involved extensive optimisations of heterologous expression of BamD and purification with the aim of progressing to crystallisation trials. We were able to generate purified folded BamD that will be used for future experiments.
Download or read book Everyday People Extraordinary Leadership written by James M. Kouzes and published by John Wiley & Sons. This book was released on 2021-01-20 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn how you can tackle everyday leadership challenges regardless of your title, position, or authority with this insightful resource A book about leadership for people who are not in formal or hierarchical leadership positions, Everyday People, Extraordinary Leadership provides readers with a comprehensive and practical approach to addressing leadership challenges, no matter the setting or circumstance. Esteemed scholars and sought-after consultants Jim Kouzes and Barry Posner adapt their trademark The Five Practices of Exemplary Leadership® framework to today’s more horizontal workplace, showing people that leadership is not about where you are in the organization; it’s about how you behave and what you do. Everyday People, Extraordinary Leadership draws on the authors’ deep well of research and practical experience to cover key subjects: The essence of making a difference in any role, setting, or situation The difference between positions of authority and leadership The importance of self-development in leadership development This book is perfectly applicable and accessible for anyone who wants to improve their own leadership potential and who isn’t yet in an official leadership role. Everyday People, Extraordinary Leadership offers authoritative new insights, original case studies and examples, and practical guidance for those individuals who want to make a difference. You supply the will, and this book will supply the way.
Download or read book Probably Approximately Correct written by Leslie Valiant and published by Basic Books (AZ). This book was released on 2013-06-04 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presenting a theory of the theoryless, a computer scientist provides a model of how effective behavior can be learned even in a world as complex as our own, shedding new light on human nature.
Download or read book If He Had Been with Me written by Laura Nowlin and published by Sourcebooks, Inc.. This book was released on 2013-04-02 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: If he had been with me everything would have been different... I wasn't with Finn on that August night. But I should've been. It was raining, of course. And he and Sylvie were arguing as he drove down the slick road. No one ever says what they were arguing about. Other people think it's not important. They do not know there is another story. The story that lurks between the facts. What they do not know—the cause of the argument—is crucial. So let me tell you...
Download or read book Emily Wilde s Encyclopaedia of Faeries written by Heather Fawcett and published by Del Rey. This book was released on 2023-01-10 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: NATIONAL BESTSELLER • A curmudgeonly professor journeys to a small town in the far north in this “incredibly fun journey through fae lands and dark magic” (NPR), the start of a heartwarming and enchanting new fantasy series. “A darkly gorgeous fantasy that sparkles with snow and magic.”—Sangu Mandanna, author of The Very Secret Society of Irregular Witches A BEST BOOK OF THE YEAR: The New York Times Book Review, NPR, PopSugar Cambridge professor Emily Wilde is good at many things: She is the foremost expert on the study of faeries. She is a genius scholar and a meticulous researcher who is writing the world’s first encyclopaedia of faerie lore. But Emily Wilde is not good at people. She could never make small talk at a party—or even get invited to one. And she prefers the company of her books, her dog, Shadow, and the Fair Folk to other people. So when she arrives in the hardscrabble village of Hrafnsvik, Emily has no intention of befriending the gruff townsfolk. Nor does she care to spend time with another new arrival: her dashing and insufferably handsome academic rival Wendell Bambleby, who manages to charm the townsfolk, muddle Emily’s research, and utterly confound and frustrate her. But as Emily gets closer and closer to uncovering the secrets of the Hidden Ones—the most elusive of all faeries—lurking in the shadowy forest outside the town, she also finds herself on the trail of another mystery: Who is Wendell Bambleby, and what does he really want? To find the answer, she’ll have to unlock the greatest mystery of all—her own heart. Book One of the Emily Wilde Series
Download or read book A Nearly Normal Family written by M. T. Edvardsson and published by Celadon Books. This book was released on 2019-06-25 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now a Netflix Limited Series "...A compulsively readable tour de force." —The Wall Street Journal New York Times Book Review recommends M.T. Edvardsson’s A Nearly Normal Family and lauds it as a “page-turner” that forces the reader to confront “the compromises we make with ourselves to be the people we believe our beloveds expect.” (NYTimes Book Review Summer Reading Issue) M.T. Edvardsson’s A Nearly Normal Family is a gripping legal thriller that forces the reader to consider: How far would you go to protect the ones you love? In this twisted narrative of love and murder, a horrific crime makes a seemingly normal family question everything they thought they knew about their life—and one another. Eighteen-year-old Stella Sandell stands accused of the brutal murder of a man almost fifteen years her senior. She is an ordinary teenager from an upstanding local family. What reason could she have to know a shady businessman, let alone to kill him? Stella’s father, a pastor, and mother, a criminal defense attorney, find their moral compasses tested as they defend their daughter, while struggling to understand why she is a suspect. Told in an unusual three-part structure, A Nearly Normal Family asks the questions: How well do you know your own children? How far would you go to protect them?
