Download or read book Termination of Transcription by Escherichia Coli RNA Polymerase at the End of the Early Regions of Bacteriophage T3 and T7 DNAs written by Norma Faye Neff and published by . This book was released on 1978 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Bacteriophages written by Richard Calendar and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been 10 years since Plenum included a series of reviews on bacte riophages, in Comprehensive Virology. Chapters in that series contained physical-genetic maps but very little DNA sequence information. Now the complete DNA sequence is known for some phages, and the se quences for others will soon follow. During the past 10 years two phages have come into common use as reagents: A phage for cloning single copies of genes, and Ml3 for cloning and DNA sequencing by the dideoxy termi nation method. Also during that period the use of alternative sigma fac tors by RNA polymerase has become established for SPOl and T4. This seems to be a widely used mechanism in bacteria, since it has been implicated in sporulation, heat shock response, and regulation of nitro gen metabolism. The control of transcription by the binding of A phage CII protein to the -35 region of the promoter is a recent finding, and it is not known how widespread this mechanism may be. This rapid progress made me eager to solicit a new series of reviews. These contributions are of two types. Each of the first type deals with an issue that is exemplified by many kinds of phages; chapters of this type should be useful in teaching advanced courses. Chapters of the second type provide comprehensive pictures of individual phage families and should provide valuable information for use in planning experiments.
Download or read book Transcription Termination by Bacteriophage T7 Rna Polymerase at Rho independent Terminators written by Shih-Tong Jeng and published by . This book was released on 1991 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt: To investigate the mechanism of transcription termination by bacteriophage RNA polymerases, templates encoding variants of the transcription-termination structure in the regulatory region of the threonine (thr) operon of Escherichia coli were used. The thr terminator comprises two distinct structural elements: a G+C-rich inverted repeat, which encodes an RNA hairpin structure, and A+T-rich regions, one of which contains a continuous sequence of template deoxyadenosine residues within which the transcript terminates. Fourteen terminator variants were analyzed by T7 RNA polymerase and we find that not only the hairpin structure itself but also its sequence influences termination. Furthermore, the formation of a hairpin in the RNA encoded by the A+T-rich regions of the attenuator is not mandatory for termination. Similar studies were also applied to T3 and Sp6 RNA polymerases. T3 RNA polymerase has very similar termination properties as T7 RNA polymerase does with both wild-type and mutated thr terminators. Sp6 RNA polymerase shows 63% termination efficiency compared to the 43% termination efficiency of T7 RNA polymerase on thr terminator, and the thr variants have less effect on Sp6 RNA polymerase than on T7 RNA polymerase. A series of seven deletion variants that successively shorten the deoxyadenosine tract in the terminator template were also analyzed. Results from these experiments with T7 and Sp6 RNA polymerases indicate that complete readthrough occurs when there are four or fewer deoxyadenosine residues. With 5 template deoxyadenosine residues there is 5 percent termination increasing to 32 percent with 8 deoxyadenosines--the value produced by the wild-type terminator. In addition, a comparison with E. coli RNA polymerase shows that bacteriophage RNA polymerases require a more perfect region of dyad symmetry and a longer deoxyadenosine tract than does the bacterial enzyme to terminate with maximum efficiency.
Download or read book The Bacteriophages written by . Stephen T. Abedon and published by Oxford University Press. This book was released on 2005-12-15 with total page 761 pages. Available in PDF, EPUB and Kindle. Book excerpt: This authoritative, timely, and comprehensively referenced compendium on the bacteriophages explores current views of how viruses infect bacteria. In combination with classical phage molecular genetics, new structural, genomic, and single-molecule technologies have rendered an explosion in our knowledge of phages. Bacteriophages, the most abundant and genetically diverse type of organism in the biosphere, were discovered at the beginning of the 20th century and enjoyed decades of used as anti-bacterial agents before being eclipsed by the antibiotic era. Since 1988, phages have come back into the spotlight as major factors in pathogenesis, bacterial evolution, and ecology. This book reveals their compelling elegence of function and their almost inconceivable diversity. Much of the founding work in molecular biology and structural biology was done on bacteriophages. These are widely used in molecular biology research and in biotechnology, as probes and markers, and in the popular method of assesing gene expression.
Download or read book Biological Regulation and Development written by Robert Goldberger and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: The motivation for us to produce a treatise on regulation was mainly our convic tion that it would be fun, and at the same time productive, to approach the subject in a way that differs from that of other treatises. We had ourselves written reviews for various volumes over the years, most of them bringing together all possible facts relevant to a particular operon, virus, or biosynthetic system. And we were not convinced of the value of such reviews for anyone but the expert in the field reviewed. We thought it might be more interesting and more instructive-for both author and reader-to avoid reviewing topics that anyone scientist might work on, but instead to review the various parts of what many different scientists work on. Cutting across the traditional boundaries that have separated the subjects in past volumes on regulation is not an easy thing to do-not because it is difficult to think of what interesting topics should replace the old ones, but because it is difficult to find authors who possess sufficient breadth of knowledge and who are willing to write about areas outside those pursued in their own laboratories. For example, no one scientist works on suppression per se. He may study the structure of suppressor tRNAs in Escherichia coli, he may study phenotypic suppression of various characters in drosophila, he may study polarity in gene expression, and so on.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1985 with total page 1094 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Canadian Journal of Microbiology written by and published by . This book was released on 1997 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2004 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Viral Messenger RNA written by Yechiel Becker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: The nucleotide sequence of the gene from which messenger RNA mole cules are transcribed is in a form that can be translated by cellular ribosomes into the amino acid sequence of a particular polypeptide, the product of the gene. The discovery of messenger RNA more than twenty years ago led to a series of studies on its organization and function in cells in the presence of infecting viruses. This volume is devoted to current studies in the field of cellular and viral messenger RNA. The studies presented provide an insight into molecular and genetic aspects of messenger RNA. Special attention was paid by the authors to the molecular organization of mRNA species, to the processing of mRNA molecules, and to the different strategies employed by DNA and RNA viruses in the synthesis of their mRNA. The ability of a virus to take over the protein-synthesizing mechanisms of an infected cell depends on its ability to produce mRNA molecules which can affect the host mRNA or utilize cellular components more efficiently. The differences between, and similarities of, the strategies of mRNA synthesis devised by various DNA and RNA viruses are described herein. This book should be of interest to all students of cellular and viral genes and scientists in the field. It is suitable as a textbook for workshops and courses on mRNA. I wish to thank the authors for their fine contributions and for their interest.
Download or read book Bioresources and Bioprocess in Biotechnology written by Shiburaj Sugathan and published by Springer. This book was released on 2017-05-26 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a compilation of detailed articles on various products and services that can be derived from bioresources through bioprocess. It offers in-depth discussions and case studies on commercially and therapeutically important enzymes, antimicrobials, anti-cancer molecules and anti-inflammatory substances. It also includes a separate section on emerging trends in bioactive substances research. This unique book is a valuable source of information for biotechnologists and bioprocess experts as well as academics and researchers who are actively involved in product and process development.
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1978 with total page 620 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Virology Abstracts written by and published by . This book was released on 1974-07 with total page 944 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies of Transcription Termination by Escherichia Coli RNA Polymerase written by Karen Marie Arndt and published by . This book was released on 1988 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Harnessing the Power of Viruses written by Boriana Marintcheva and published by Academic Press. This book was released on 2017-11-13 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Harnessing the Power of Viruses explores the application of scientific knowledge about viruses and their lives to solve practical challenges and further advance molecular sciences, medicine and agriculture. The book contains virus-based tools and approaches in the fields of: i) DNA manipulations in vitro and in vivo; ii) Protein expression and characterization; and iii) Virus- Host interactions as a platform for therapy and biocontrol are discussed. It steers away from traditional views of viruses and technology, focusing instead on viral molecules and molecular processes that enable science to better understand life and offer means for addressing complex biological phenomena that positively influence everyday life. The book is written at an intermediate level and is accessible to novices who are willing to acquire a basic level of understanding of key principles in molecular biology, but is also ideal for advanced readers interested in expanding their biological knowledge to include practical applications of molecular tools derived from viruses. - Explores virus-based tools and approaches in DNA manipulation, protein expression and characterization and virus-host interactions - Provides a dedicated focus on viral molecules and molecular processes that enable science to better understand life and address complex biological phenomena - Includes an overview of modern technologies in biology that were developed using viral components/elements and knowledge about viral processes
Download or read book Comprehensive Dissertation Index written by and published by . This book was released on 1984 with total page 818 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1973- include the following subject areas: Biological sciences, Agriculture, Chemistry, Environmental sciences, Health sciences, Engineering, Mathematics and statistics, Earth sciences, Physics, Education, Psychology, Sociology, Anthropology, History, Law & political science, Business & economics, Geography & regional planning, Language & literature, Fine arts, Library & information science, Mass communications, Music, Philosophy and Religion.
Download or read book Biological Regulation and Development written by Robert F. Goldberger and published by Springer. This book was released on 1979-03 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: Band 1.
Download or read book Annual Review of Genetics written by and published by . This book was released on 1987 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: Publishes original critical reviews of the significant literature and current development in genetics.
