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Book The Operon

    Book Details:
  • Author : Jeffrey H. Miller
  • Publisher :
  • Release : 1980
  • ISBN :
  • Pages : 488 pages

Download or read book The Operon written by Jeffrey H. Miller and published by . This book was released on 1980 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book An Analysis of the Effect of Mutations in the Leader Region of the Threonine Operon of Escherichia Coli on Attenuation Or Transcription Termination

Download or read book An Analysis of the Effect of Mutations in the Leader Region of the Threonine Operon of Escherichia Coli on Attenuation Or Transcription Termination written by Harlan Bernard Scott (II) and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The expression of the threonine operon of Escherichia coli is controlled by attenuation. An open translational reading frame in the leader region preceding the structural genes contains 21 codons with eight threonine and four isoleucine codons. The model for attenuation proposes that regulation depends on the translation of the 21 amino acid leader peptide. Oligonucleotide-directed mutagenesis was used to introduce mutations into the leader region. Thirty-nine mutants were isolated. The effects of individual mutations on regulation were studied by making fusions to the lacZ gene, reducing the plasmid copy number, and assaying for the $beta$-galactosidase activity. Five mutants with two frameshift mutations each were constructed. The double frameshift mutants changed the leader peptide in two ways. First, the mutants had differing numbers of threonine and isoleucine codons. Second, the mutants had the threonine and isoleucine codons changed to differing numbers of histidine codons. Changing three threonine and one isoleucine codons abolished regulation by excess threonine and isoleucine. The presence of two histidine codons derepressed the operon in a hisT::Km background. Three mutations that would be predicted to block the translation of the normal leader peptide were constructed. All three mutants showed loss of regulation. The attenuator in the leader region encodes a rho-independent terminator. The terminator consists of a stem-loop structure with six bases in the loop and eight basepairs in the stem. The stem-loop is followed by nine rU residues and preceded by six rA residues. Various mutations in the threonine attenuator were assayed for their effects on transcription termination efficiency. As the number of rU residues in the terminator decreased from eight to one, the termination efficiency decreased. As the number of rA residues preceding the stem decreased from eight to one, the termination efficiency slightly increased. The sequence of the loop was also found to have a slight affect on termination efficiency. The sequences distal to the rU residues, which were non-transcribed, also had a slight affect on termination efficiency. Finally, a combination of changes in the stem with a change either in the loop or sequences distal to the rU residues had an affect on termination efficiency.

Book Termination of Transcription by Escherichia Coli RNA Polymerase at the End of the Early Regions of Bacteriophage T3 and T7 DNAs

Download or read book Termination of Transcription by Escherichia Coli RNA Polymerase at the End of the Early Regions of Bacteriophage T3 and T7 DNAs written by Norma Faye Neff and published by . This book was released on 1978 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Regulation of Gene Expression in Escherichia Coli

Download or read book Regulation of Gene Expression in Escherichia Coli written by E. C. C. Lin and published by Springer. This book was released on 1996-06-27 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt: This up-to-date guide focuses on the understanding of key regulatory mechanisms governing gene expression in Escherichia coli. Studies of E. coli not only provide the first models of gene regulation, but research continues to yield different control mechanisms.

Book Regulation of the Bacillus Subtilis Tryptophan Operon by Transcription Attenuation

Download or read book Regulation of the Bacillus Subtilis Tryptophan Operon by Transcription Attenuation written by Mitzi Irene Kuroda and published by . This book was released on 1987 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Transcription of the Tryptophan Operon in Nonsense Mutants of Escherichia Coli

Download or read book Transcription of the Tryptophan Operon in Nonsense Mutants of Escherichia Coli written by and published by . This book was released on 1970 with total page 20 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Bacillus Subtilis and Its Closest Relatives

Download or read book Bacillus Subtilis and Its Closest Relatives written by A. L. Sonenshein and published by . This book was released on 2002 with total page 664 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focusing on issues of gene organization, regulation, and evolution in the context of the whole life of the cell, this new volume complements the editors' classic 1993 volume Bacillus subtilis and Other Gram-Positive Bacteria. Building upon the previous edition, Bacillus subtilis and Its Closest Relatives contains an updated annotation of the complete B. subtilis genome and includes a unique compilation of major pathways of metabolism and macromolecular synthesis, correlating genes and proteins and assigning new functions to many genes. It also provides clear explanations of the major regulatory mechanisms that are unique to gram-positive bacteria as well as an overview of their special properties. This essential reference offers detailed, current information and is valuable reading for microbiologists, biotechnologists, and students.

Book Control of Transcription in Coliphage Lambda

Download or read book Control of Transcription in Coliphage Lambda written by John Stuart Salstrom and published by . This book was released on 1977 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Prokaryotic Metabolism and Physiology

Download or read book Prokaryotic Metabolism and Physiology written by Byung Hong Kim and published by Cambridge University Press. This book was released on 2019-05-16 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extensive and up-to-date review of key metabolic processes in bacteria and archaea and how metabolism is regulated under various conditions.

Book Essentials of Medical Biochemistry

Download or read book Essentials of Medical Biochemistry written by Chung Eun Ha and published by Academic Press. This book was released on 2011-01-28 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expert biochemist N.V. Bhagavan's new work condenses his successful Medical Biochemistry texts along with numerous case studies, to act as an extensive review and reference guide for both students and experts alike. The research-driven content includes four-color illustrations throughout to develop an understanding of the events and processes that are occurring at both the molecular and macrolecular levels of physiologic regulation, clinical effects, and interactions. Using thorough introductions, end of chapter reviews, fact-filled tables, and related multiple-choice questions, Bhagavan provides the reader with the most condensed yet detailed biochemistry overview available. More than a quick survey, this comprehensive text includes USMLE sample exams from Bhagavan himself, a previous coauthor. - Clinical focus emphasizing relevant physiologic and pathophysiologic biochemical concepts - Interactive multiple-choice questions to prep for USMLE exams - Clinical case studies for understanding basic science, diagnosis, and treatment of human diseases - Instructional overview figures, flowcharts, and tables to enhance understanding

Book Sequence     Evolution     Function

    Book Details:
  • Author : Eugene V. Koonin
  • Publisher : Springer Science & Business Media
  • Release : 2013-06-29
  • ISBN : 1475737831
  • Pages : 482 pages

Download or read book Sequence Evolution Function written by Eugene V. Koonin and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sequence - Evolution - Function is an introduction to the computational approaches that play a critical role in the emerging new branch of biology known as functional genomics. The book provides the reader with an understanding of the principles and approaches of functional genomics and of the potential and limitations of computational and experimental approaches to genome analysis. Sequence - Evolution - Function should help bridge the "digital divide" between biologists and computer scientists, allowing biologists to better grasp the peculiarities of the emerging field of Genome Biology and to learn how to benefit from the enormous amount of sequence data available in the public databases. The book is non-technical with respect to the computer methods for genome analysis and discusses these methods from the user's viewpoint, without addressing mathematical and algorithmic details. Prior practical familiarity with the basic methods for sequence analysis is a major advantage, but a reader without such experience will be able to use the book as an introduction to these methods. This book is perfect for introductory level courses in computational methods for comparative and functional genomics.