Download or read book Temporal Changes in the Microbial Community of a PAH contaminated Soil During Bench top Bioremediation written by Caroline Gayle Rose and published by . This book was released on 2010 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microbial Community Structure During Bioremediation of Polycyclic Aromatic Hydrocarbon PAH Contaminated Soil written by Fengxiao Zhu and published by . This book was released on 2015 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microbial Community Dynamics During the Bench scale Bioremediation of Petroleum contaminated Soil written by Kristin M. Fitzgerald and published by . This book was released on 1999 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biotechnological Advances in Bioremediation of Heavy Metal Polluted Soils written by Ying Ma and published by Frontiers Media SA. This book was released on 2022-11-15 with total page 133 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bioremediation of Pollutants in Soil and Water written by Brian S. Schepart and published by ASTM International. This book was released on 1995 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reproduced typescripts of 17 papers from a symposium in Fort Worth, Texas, in October 1993 discuss technical aspects of biological methods for cleaning up polluted sites. They cover the economics of bioremediation compared with other methods, the special problems associated with polynuclear aromatic

Download or read book Changes in Populations of Phenanthrene degrading Bacteria During the Bioremediation of Polycyclic Aromatic Hydrocarbon Contaminated Soil written by Kaysha Odell Stein and published by . This book was released on 1999 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Utilization of Bioremediation to Reduce Soil Contamination Problems and Solutions written by Václav Sasek and published by Springer Science & Business Media. This book was released on 2003-02-28 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the NATO Advanced Research Workshop held in Prague, Czech Republic, 14-19 June 2000

Download or read book The Molecular Based Methods Used for Studying Bacterial Diversity in Soils Contaminated with PAHs The Review written by Anna Gałązka and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Soil contamination could adversely affect microbial diversity, and perhaps also above- and below-ground ecosystem functioning. It is important to study microbial diversity not only for basic scientific research, but also to understand the link between diversity and community structure and function in the pollution site. The study of microbial diversity and their function in contaminated soil creates a serious problem because they observed significant limitations in methodology and taxonomy of this group. Methodology for the determination of bacterial diversity does not include their function in the soil and other environment areas. Microbes are known for their catabolic activity in bioremediation, but changes in microbial communities are still unpredictable. The bioremediation of a pollutant and its rate depend on the environmental conditions, number and type of the microorganisms, nature and chemical structure of the chemical compound being degraded. However, molecular methods have been used to study soil bacterial communities. While many anthropogenic activities, such as city development, agriculture, and use of pollution, can potentially affect soil microbial diversity, it is unknown how changes in microbial diversity can influence below-ground and above-ground ecosystems. There are problems associated with studying bacterial diversity in soil. These arise not only from methodological limitations, but also from a lack of taxonomic knowledge. Methods to measure microbial diversity in soil can be categorized into two groups: biochemical-based techniques and molecular-based techniques. But more common for studying microbial diversity in soil contaminated with polycyclic aromatic hydrocarbons are the molecular methods.

Download or read book Advances in Applied Bioremediation written by Ajay Singh and published by Springer Science & Business Media. This book was released on 2009-07-30 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioremediation is a rapidly advancing field and the technology has been applied successfully to remediate many contaminated sites. The goal of every soil remediation method is to enhance the degradation, transformation, or detoxification of pollutants and to protect, maintain and sustain environmental quality. Advances in our understanding of the ecology of microbial communities capable of breaking down various pollutants and the molecular and biochemical mechanisms by which biodegradation occurs have helped us in developing practical soil bioremediation strategies. Chapters dealing with the application of biological methods to soil remediation are contributed from experts – authorities in the area of environmental science including microbiology and molecular biology – from academic institutions and industry.

Download or read book Microbial Processes for Bioremediation written by Robert E. Hinchee and published by . This book was released on 1995 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: These articles focus on methods for optimizing the effectiveness of microorganisms for biodegradation. This volume includes discussions of the biodegradation and toxicity of PAH-, PCP-, PCB-, and BTEX-contaminated soil and aqueous sediments. Both bench-scale and pilot-scale studies deal with microbial issues such as surfactant-enhanced aromatic hydrocarbon degradation, nutrient addition and bioavailability, and various dispersion techniques. Selected case studies include discussions on pre-culture and consortium biodegradation of various petroleum hydrocarbons.

Download or read book Long Term Impacts of a Genetically Engineered GEM and Polycyclic Aromatic Hydrocarbons PAHs on Soil Bacterial Communities written by Xiaoci Ji and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbes capable of polyaromatic hydrocarbon (PAH) biodegradation can be used to remediate soils contaminated with these persistent pollutants. To monitor in situ PAH-biodegradation, the bioluminescent bio-reporter Pseudomonas fluorescens HK44, containing a lux luminescent gene cassette inserted into its naphthalene degradation operon, was released into PAH-contaminated soil in lysimeters in 1996. Three treatments were imposed: strain HK44 mixed with PAH-contaminated soil (PAH+, HK44+; n=3); strain HK44 mixed with uncontaminated soil (PAH–, HK44+; n=2) and PAH-contaminated soil alone (PAH+, HK44–; n=1). The objective of this study was to assess the long term impacts of these treatments on the indigenous soil bacterial community structure in the lysimeters. In 2010, 14 years after experiment initiation, replicate soil cores were taken from each lysimeter. Soil bacterial community structures were determined by 454 pyrosequencing of 16S rRNA gene amplicons. Even though PAH concentrations fell below detectable levels within the first couple years of the lysimeter experiment, PAH+ lysimeters showed significantly higher soil organic matter content (1.30 ± 0.23%) than PAH– lysimeters (0.81 ± 0.08%). 16S rRNA gene libraries reveal that there was a change in the bacterial community structure in PAH+ compared to PAH– lysimeters: 9.59% of OTUs (operational taxonomic units) were shared between PAH+ lysimeters while only 4.08% were shared between PAH+ and PAH– lysimeters. Multivariate ordination and cluster analysis, and phylogenetic tree-based analysis indicate that communities fell into three clusters: lysimeter 1 and 2 (both PAH+, HK44+); lysimeter 4 (PAH+, HK44+) and 6 (PAH+, HK44–); and lysimeter 3 and 5 (both PAH–, HK44+). Therefore over the long term, the addition of PAHs was more influential on bacterial community structure than the introduction of a GEM.

Download or read book Molecular Analysis of Mycobacterium Community Shifts in Soil During the Biodegradation of Polycyclic Aromatic Hydrocarbons written by Pui Yi Cheung and published by . This book was released on 2001 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cing exogenous PAH-degraders, nutrient- and surfactant-amendments, and acclimation of soil microorganisms to PAHs, on PAH mineralization patterns and the degradative potential of the community were measured and correlated with Mycobacterium population dynamics. Results indicated that inoculation of soil with exogenous PAH-degrader had both positive and negative effects on PAH degradation, probably due to competition with indigenous microorganisms. Both nutrients and surfactants enhanced the rate and level of PAH degradation in contaminated soils, but the soil that had the highest level of mineralization had the least changes in Mycobacterium community structure. Pre-exposure of soil microorganisms to a PAH decreased the acclimation period and increased the initial rates for subsequent degradation of the same or a different PAH, in some soils. Contamination of soil samples with PAHs all resulted in Mycobacterium community shifts, as revealed by PCR-TGGE. The results obtained provided insight into understanding the ecological limitations to effective bioremediation of contaminated environmental sites.

Download or read book Assessment of Microbial Community Changes and Limiting Factors During Bioremediation of Hydrocarbon polluted Soil with New Miniaturized Physiological Methods written by Karin Kaufmann and published by . This book was released on 2004 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Assessment of Microbial Community Changes and Limiting Factors During Bioremediation of Hydrocarbon pollluted Soil with New Miniaturized Physiological Methods written by K. Kaufmann and published by . This book was released on 2004 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book PAH Impact on the Sediment Bacterial Community written by SF. Pikett and published by . This book was released on 1995 with total page 15 pages. Available in PDF, EPUB and Kindle. Book excerpt: The potential for bioremediating polycyclic aromatic hydrocarbons (PAHs), the major constituents of creosote, will depend on the response of the sediment bacterial community. The microbial community in sediments from a creosote-polluted freshwater lake was studied. The aim of this study was to assess the impact of PAH contamination on the sediment bacterial community. Sediment samples were taken at selected sites around the lake and upstream at an unpolluted site. Samples were analyzed for selected PAH concentrations. The numbers of aerobic heterotrophic bacteria isolated after growth on peptone yeast extract agar, peptone yeast extract with added creosote agar, and a minimal media with added creosote agar were determined for each site. The response of different bacterial physiological types to PAH contamination in sediment ecosystems was assessed through the use of a gel-stabilized model system. It was apparent that the PAH contamination had a marked effect on the bacterial community of these freshwater sediments. Such effects may influence the cycling of nutrients within sediments, e.g., the nitrogen and sulfur cycle, and the potential for in situ bioremediation.

Download or read book Environmental Quality Objectives in the Netherlands written by Risk Assessment and Environmental Qualithy Division. Directorate for Chemicals. External Safety and Radiaiton Protection. Ministry of Housing Spatial Planning and the Environment and published by . This book was released on 1994 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bioremediation of Petroleum Hydrocarbons written by Ali Akbari and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Indigenous, cold-adapted, hydrocarbon-degrading bacteria have been frequently detected in soils from cold region sites suggesting bioremediation, as a cost-effective remediation technology is potentially viable for cleanup of contaminated sites in cold regions. Previous studies on bioremediation of petroleum hydrocarbons in cold regions have been conducted with coarse textured soils. However, fine-grained or clayey soils are commonly found in several cold regions. The overall objective of this research was to investigate the factors controlling biodegradation of aged, clayey petroleum hydrocarbon contaminated soils from a sub-arctic site, under relevant temperature regimes. The biodegradation rates and extents of petroleum hydrocarbons were investigated for a clayey site contaminated soil in four pilot scale biopiles maintained at 15°C. Non-volatile petroleum hydrocarbons (C16-C32) were biodegraded 24 to 38% under continuous aeration and with amendments of 17.5% or 23.5% moisture and also 17.5% moisture with 95 mg-N/ kg soil. In contrast, statistically significant biodegradation of the non-volatile hydrocarbon fraction was not observed in a biopile tank containing soils amended with 23.5% moisture content and 1340 mg-N/ kg soil. While a significant shift occurred in soil microbial community over 60 days in the biopile tank amended with 23.5% moisture only, there was no change in the microbial community of soil samples amended with a similar level of moisture and 1340 mg-N/ kg soil, indicating that high amounts of nitrogen amendment inhibited bioremediation.This study investigates the effect of diurnal temperature variations on the biodegradation of petroleum hydrocarbon contaminated soil typical for the site from northern Canada from which contaminated soils were obtained. The extents of biodegradation of petroleum hydrocarbons after 70 days, in systems incubated at temperatures varying between 5 °C to 15 °C daily (representative of daily high and low temperatures) were similar to systems incubated at constant temperature of 15 °C, and were significantly higher than systems incubated at 5 °C. The soil microbial community of systems with daily changes in temperatures between 5 °C to 15 °C was similar to those incubated at 15 °C, but significantly different than those incubated at 5 °C, based on both 16S rRNA and alkB gene analyses. The results suggest that the highest temperature (15 °C) to which the soil was frequently exposed to, determined the microbial community profile in the site soil, and periods of low temperatures did not reduce the overall biodegradation efficiency or the microbial community compared to that maintained solely at 15 °C.Experiments were conducted to investigate if hexadecane, a biodegradable but effectively insoluble hydrocarbon phase, was accessible to, and degraded by hydrocarbon degrading bacteria when the hexadecane-water interface was separated from the microbial culture by membranes with specific pore sizes. In these systems, bacteria were unable to degrade hexadecane when the membrane pore size was 0.4 μm or 3 μm, but was able to degrade hexadecane when separated by pore sizes of 12 μm. To investigate the implications of these results to aggregates from clayey and sandy soils, the in-situ aggregate micro-structure of representative soil aggregates from petroleum contaminated clayey soil and petroleum contaminated sandy soil were compared by micro-scale computed tomography (micro CT) imaging. The "bioaccessible porosity" defined as fraction of aggregate volume corresponding to pores larger than 4 μm was comparable in case of clayey (26-27%) and sandy (24%) aggregates." --