Download or read book Tandem Mass Spectrometry written by Ana Varela Coelho and published by BoD – Books on Demand. This book was released on 2013-05-29 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tandem Mass Spectrometry - Molecular Characterization presents a comprehensive coverage of theory, instrumentation and description of experimental strategies and MS/MS data interpretation for the structural characterization of relevant molecular compounds. The areas covered include the analysis of drugs, metabolites, carbohydrates and protein post-translational modifications. The book series in Tandem Mass Spectrometry serves multiple groups of audiences; professional (academic and industry), graduate students and general readers interested in the use of modern mass spectrometry in solving critical questions of chemical and biological sciences.
Download or read book Tandem Mass Spectrometry of Lipids written by Robert C Murphy and published by Royal Society of Chemistry. This book was released on 2014-12-02 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: The emerging field of lipidomics has been made possible because of advances in mass spectrometry, and in particular tandem mass spectrometry of lipid ions generated by electrospray ionization. The ability to carry out basic biochemical studies of lipids using electrospray ionization is predicated upon understanding the behaviour of lipid derived ions following collision induced decomposition and mechanisms of product ion formation. During the past 20 years, a wealth of information has been generated about lipid molecules that are now analysed by mass spectrometry, however there is no central source where one can obtain basic information about how these very diverse biomolecules behave following collisional activation. This book brings together, in one volume, this information so that investigators considering using tandem mass spectrometry to structurally characterize lipids or to quantitate their occurrence in a biological matrix, will have a convenient source to review mechanism of decomposition reactions related to the diversity of lipid structures. A separate chapter is devoted to each of seven major lipid classes including fatty acids, eicosanoids and bioactive lipid mediators, fatty acyl esters and amides, glycerol esters, glycerophospholipids, sphingolipids, and steroids. Mechanistic details are provided for understanding the pathways of formation of major product ions and ions used for structural characterization. In most cases specific ancillary information has been critical to understand the pathways, including isotope labeling and high resolution analysis of precursor and product ions. For a few specific examples such data is missing and pathways are proposed as a means to initiate further mass spectral experiments to prove or disprove pathway hypotheses. While this work largely centres on the lipid biochemistry of animal (mammalian) systems, general principles can be taken from the specific examples and applied to lipid biochemistry found in plants, fungi, prokaryotes and archeal organisms.
Download or read book Tandem Mass Spectrometry and Ion molecule Interactions written by Asoka Ranasinghe and published by . This book was released on 1992 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of Phospholipid Molecular Species by Means of HPLC Tandem Mass Spectrometry written by Natale G. Frega and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Sequencing and Identification Using Tandem Mass Spectrometry written by Michael Kinter and published by John Wiley & Sons. This book was released on 2005-04-12 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: * Peptide fragmentation and interpretation of product ion spectra * Basic polyacrylamide gel electrophoresis * Preparation of protein digests for sequencing experiments * Mass spectrometric analysis using capillary liquid chromatography * Techniques for protein identification by database searches * Characterization of modified peptides using tandem mass spectrometry And much more
Download or read book Neuroproteomics written by Oscar Alzate and published by CRC Press. This book was released on 2009-10-26 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg
Download or read book Mass Spectrometry written by Dominic M. Desiderio and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: The current volume reviews novel instrumentation and techniques as applied to the analysis of diacylglycerylphospholipids, modifications to DNA, and the characterization of variant hemoglobins. It reflects the flexible, broad-based approaches mass spectroscopists apply to clinical and biomedical challenges.
Download or read book Tandem Mass Spectrometry written by Fred W. McLafferty and published by Wiley-Interscience. This book was released on 1983 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1909 edition. Excerpt: ... well satisfied with the impression he had made upon all concerned. Bertha had mounted to her room and waited, first sending her maid away. Presently her door had been opened and her husband entered, and for an hour she had been listening to his outburst of distorted jealousy. The attack was finally losing its force, and turning a shoulder she seated herself at the dressingtable and began to cut the threads with which a jeweled ornament was sewn to her bodice. In the mirror his face looked at her with all the sneering, abandoned malevolence to which she was so accustomed. "If you are quite through, I will ring for my maid," she said at last. "Im through for now, but you and your discarded lover--your former kept man" She cut a thread very carefully. "Ill have the truth from one of you or the other--do you hear me, you common hussy? You vile street" His elbow brushed some roses. He dashed the vase to the floor and stamped petals and Sevres into the rug. She unhooked her pearl collar, and drew it from her neck. "All I want you to do," said the hoarse whine behind her--the mans anger was evidently ex "In the mirror his face looked at her with all the sneering. malevolence to which she was so accustomed." hausted on the vase--"all I ask of you is just to confess. Tell me that he was your lover and I will ask you no more--just say it--tell me that he was--tell me that, and Ill be satisfied." She laid the collar in its case. Would a lie" soothe him? She knew it would not. He would want details. She smiled with the thought of the whole mad, foolish, trivial thing. With a scream he clutched her. She twisted away, but his fist in her laces tore them to the girdle. Still holding the torn strip, he thrust his twitching face close to hers. Her...
Download or read book Mass Spectrometry Data Analysis in Proteomics written by Rune Matthiesen and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. The volume outlines available bioinformatics programs, algorithms, and databases available for MS data analysis. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters.
Download or read book Interpretation of MS MS Mass Spectra of Drugs and Pesticides written by Wilfried M. A. Niessen and published by John Wiley & Sons. This book was released on 2017-01-09 with total page 799 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides comprehensive coverage of the interpretation of LC–MS–MS mass spectra of 1300 drugs and pesticides Provides a general discussion on the fragmentation of even-electron ions (protonated and deprotonated molecules) in both positive-ion and negative-ion modes This is the reference book for the interpretation of MS–MS mass spectra of small organic molecules Covers related therapeutic classes of compounds such as drugs for cardiovascular diseases, psychotropic compounds, drugs of abuse and designer drugs, antimicrobials, among many others Covers general fragmentation rule as well as specific fragmentation pathways for many chemical functional groups Gives an introduction to MS technology, mass spectral terminology, information contained in mass spectra, and to the identification strategies used for different types of unknowns
Download or read book Comprehensive Natural Products II written by and published by Elsevier. This book was released on 2010-03-05 with total page 7944 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work presents a definitive interpretation of the current status of and future trends in natural products—a dynamic field at the intersection of chemistry and biology concerned with isolation, identification, structure elucidation, and chemical characteristics of naturally occurring compounds such as pheromones, carbohydrates, nucleic acids, and enzymes. With more than 1,800 color figures, Comprehensive Natural Products II features 100% new material and complements rather than replaces the original work (©1999). Reviews the accumulated efforts of chemical and biological research to understand living organisms and their distinctive effects on health and medicine Stimulates new ideas among the established natural products research community—which includes chemists, biochemists, biologists, botanists, and pharmacologists Informs and inspires students and newcomers to the field with accessible content in a range of delivery formats Includes 100% new content, with more than 6,000 figures (1/3 of these in color) and 40,000 references to the primary literature, for a thorough examination of the field Highlights new research and innovations concerning living organisms and their distinctive role in our understanding and improvement of human health, genomics, ecology/environment, and more Adds to the rich body of work that is the first edition, which will be available for the first time in a convenient online format giving researchers complete access to authoritative Natural Products content
Download or read book Advancing Tandem Mass Spectrometry for Characterization of Complex Biopolymers written by Trenton M. Peters-Clarke and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation details innovations in mass spectrometry-based instrumentation that expand our ability to survey biopolymers, including proteins, peptides, and oligonucleotides, with increased sensitivity, speed, and depth. Chapter 1 provides an overview of mass spectrometry instrumentation at the leading edge of proteomics and for nucleic acid analysis. In Chapter 2, the optical-fiber enabled coupling of a mass spectrometer to an infrared laser improves proteomic characterization. For Chapter 3, electron transfer dissociation-based proteomics is investigated and inefficiencies of related ion-ion reactions are characterized. Chapter 4 introduces a novel nucleic acid sequencing technology with activated ion-negative electron transfer dissociation to profile RNA molecules and their modifications. In Chapter 5, tandem mass spectrometry comprehensively characterizes heavily-modified RNA therapeutics. Chapter 6 focuses on a proteome-wide analysis of the bacterial post-translational modification O-mycoloylation. In Chapter 7, a novel tandem mass spectrometry strategy, termed activated ion-tandem mass tags, is revealed, boosting quantitative reporter ion generation. Chapter 8 expands upon AI-TMT for quantitative single-cell proteomics, realizing sensitivity gains which drive a greater dynamic range of quantification. In Chapter 9, a novel mass spectrometer enables the one hour human proteome and we explore one minute proteome analyses, pushing the limits of high-throughput proteome analysis. Chapter 10 discusses emergent proteomic techniques and suggests future directions of the field.
Download or read book MALDI TOF and Tandem MS for Clinical Microbiology written by Haroun N. Shah and published by John Wiley & Sons. This book was released on 2017-03-31 with total page 850 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights the triumph of MALDI-TOF mass spectrometry over the past decade and provides insight into new and expanding technologies through a comprehensive range of short chapters that enable the reader to gauge their current status and how they may progress over the next decade. This book serves as a platform to consolidate current strengths of the technology and highlight new frontiers in tandem MS/MS that are likely to eventually supersede MALDI-TOF MS. Chapters discuss: Challenges of Identifying Mycobacterium to the Species level Identification of Bacteroides and Other Clinically Relevant Anaerobes Identification of Species in Mixed Microbial Populations Detection of Resistance Mechanisms Proteomics as a biomarker discovery and validation platform Determination of Antimicrobial Resistance using Tandem Mass Spectrometry
Download or read book Mass Spectrometry written by Mahmood Aliofkhazraei and published by BoD – Books on Demand. This book was released on 2017-06-07 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry is an analytical technique that can be used for the structural characterization and quantification of a wide range of molecules. The technique is extensively used by chemists for the analysis of small and volatile organic compounds. Mass spectrometry has long been an important technique for the identification of materials ranging from pure compounds to complex mixtures. Mass spectrometry can be used to determine molecular weight of compounds or using different ionization conditions, can provide more structural details through the analysis of fragmentation patterns. This level of detail can be attained for pure compounds and some mixtures. Mass spectrometry can also be combined with separation techniques such as gas chromatography or liquid chromatography to allow more complex mixtures to be examined. These hyphenated techniques provide a range of options for the characterization of complex materials.
Download or read book MALDI Mass Spectrometry for Synthetic Polymer Analysis written by Liang Li and published by John Wiley & Sons. This book was released on 2009-10-22 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Practices of Polymer Mass Spectrometry helps readers acquire the skills necessary for selecting the optimal methods, handling samples, analyzing the data, and interpreting the results of the mass spectrometry of polymers. This guide describes the principles of polymer MS and best practices in polymer characterization. It discusses different approaches, including MALDI, ESI, TOF MS, and FT-MS. It provides a guide to developing appropriate sample preparation protocols for different polymers. Complete with examples of applications and experiments, this is an excellent reference for scientists, researchers, graduate students, and others.
Download or read book Analyzing Biomolecular Interactions by Mass Spectrometry written by Jeroen Kool and published by John Wiley & Sons. This book was released on 2015-05-04 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.
Download or read book Clinical Applications of Mass Spectrometry in Biomolecular Analysis written by Uttam Garg and published by Humana. This book was released on 2015-11-25 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and no need for specialized reagents. The techniques described are a must for the measurement of many clinically relevant analytes in the fields of drug analysis, endocrinology, and inborn errors of metabolism. Each chapter provides a brief introduction about a specified analyte, followed by detailed instructions on the analytical protocol. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols is a great resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.
