Download or read book Synthesis Characterization and Optical Properties of Colloidal Organic Dye containing Fluorescent Core shell Silica Nanoparticles written by Erik Herz and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since fluorescence was first observed in a solution containing quinine sulfate in 1845, methods employing fluorescent materials have abounded. Today, fluorescent probes are widely used in many fields, including biological imaging and security. For all applications brightness, stability, size control and compatibility are key parameters for probe design. Recent research to address these parameters has been focused on synthesizing nanoparticle materials. A class of those materials, core-shell silica nanoparticles, covalently encapsulating organic dyes within the core, have been found to be particularly effective in meeting the need for brighter, more stable probes, while also providing size and compatibility control. This dissertation will describe how I have built on previous research by focusing on the synthesis, characterization and optical properties of core-shell silica nanoparticles that span the full spectral range, from ultraviolet to near-infrared emission. Through scattering corrected comparisons of coumarin dye-containing nanoparticles and dye in aqueous solution, relative quantum yield enhancements of an order of magnitude were measured. Similar brightness enhancements were observed when large Stokes-shift dyes were incorporated into the particles. By creating a graded series of Stokes-shifted particles, single-excitation functionality was added to the fluorescent core-shell silica nanoparticle platform, to allow for spectral multiplexing. Since both light scattering and autofluorescence backgrounds in biological samples tend to be stronger at shorter wavelengths, new probe design has swiftly moved toward near-infrared (NIR) excitation and emission. To this end, NIR fluorescent silica nanoparticles with emissions up to 800nm were synthesized and dye structure-optical property correlations were investigated. Using a self-built fluorescence correlation spectroscopy (FCS) instrument, cyanine dye containing particles of the NIR series were investigated in further depth. The FCS allows for measurement of the size, brightness and concentration of the particles in solution and, with small modifications to the beam-path also provides information on the fluorescence stability of the particles in solution, which is of particular importance in NIR probes. Photostability in aqueous solution was investigated for the NIR dye Cy5 and compared to the dye incorporated into particles, leading to findings that will impact future probe designs.
Download or read book Colloidal Quantum Dot Optoelectronics and Photovoltaics written by Gerasimos Konstantatos and published by Cambridge University Press. This book was released on 2013-11-07 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Captures the most up-to-date research in the field, written in an accessible style by the world's leading experts.
Download or read book Development and Applications of Fluorescent Core shell Silica Nanoparticles for Bioimaging and Sensing written by Andrew Arthur Burns and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nano-sized fluorescent materials have demonstrated great potential in the emergent fields of nanobiotechnology and nanomedicine. To create effective probes for these applications, high brightness, photostability and size control are key parameters for particle design. Core-shell silica nanoparticles incorporating covalently bound dyes in the particle core have been shown to exhibit significant enhancements in dye brightness and photostability while allowing independent control of particle size, color and surface chemistry. Building on this platform, the first part of this dissertation explores a family of quantitative chemical sensors based on the incorporation of analyte-sensitive dyes into the particle shell. By co-localizing sensor and reference dye molecules onto a single particle, local analyte concentration changes can be discerned from local sensor concentration variations by comparison of the sensor and reference intensities facilitating quantitative "ratiometric" chemical imaging. The core-shell architecture is ideal for this application because it sequesters the reference dye in the particle core, while allowing analyte interactions with the sensor dyes near the particle surface. Traditional Stöber-derived sol-gel routes as well as reverse micelle-based methods were employed to generate pH- and Ca+2-sensitive nanoparticles. Specific sensor architectures were applied to intracellular as well as large-scale functional volumetric imaging of intact biofilms and their metabolic activities. To fully realize the potential of fluorescent nanomaterials for biomedical applications, one of the key obstacles is clearance from the body. The second part of this dissertation investigates several of the key parameters for effective clearance (size and surface chemistry) and highlights the development of a series of particles capable of rapid and efficient bodily clearance and demonstrates their efficacy in mice as a model system.
Download or read book Functional Dyes written by Sung-Hoon Kim and published by Elsevier. This book was released on 2006-03-08 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last 10 years organic dyes, traditionally used for coloring textiles and other materials, have become increasingly important in the hi-tech industries of electronics and optoelectronics. They can be used in optical data storage, new solar cells and biomedical sensors.Functional Dyes discusses the synthesis of these new, high-value dyes and pigments as well as their applications and performance. The chapters are arranged so that the reader logically advances from the fundamental concepts to more practical aspects of the technology in which they are used.In providing the reader with current information on functional dye chemistry, as well as important developments within the field, Functional Dyes is a valuable information source for dye and material chemists, researchers and graduates, who want a summary of the key advances in the field over the last 10 years and an authoritative view on future developments. * Provides a broad introduction to the science technology of the functional dye application* Reviews recent advances on synthesis and characteristics of the functional dyes and their applications* Is a valuable information source for dye and material chemists and researchers
Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Download or read book Lanthanide Luminescence written by Pekka Hänninen and published by Springer Science & Business Media. This book was released on 2011-06-21 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lanthanides have fascinated scientists for more than two centuries now, and since efficient separation techniques were established roughly 50 years ago, they have increasingly found their way into industrial exploitation and our everyday lives. Numerous applications are based on their unique luminescent properties, which are highlighted in this volume. It presents established knowledge about the photophysical basics, relevant lanthanide probes or materials, and describes instrumentation-related aspects including chemical and physical sensors. The uses of lanthanides in bioanalysis and medicine are outlined, such as assays for in vitro diagnostics and research. All chapters were compiled by renowned scientists with a broad audience in mind, providing both beginners in the field and advanced researchers with comprehensive information on on the given subject.
Download or read book Bijels written by Paul S Clegg and published by Royal Society of Chemistry. This book was released on 2020-03-27 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bicontinuous interfacially jammed emulsion gels, now commonly termed 'bijels', are a class of soft materials, in which interpenetrating, continuous domains of two immiscible fluids are maintained in a rigid arrangement by a jammed layer of colloidal particles at their interface. Such gels have unusual material properties that promise exciting applications across diverse fields from energy materials and catalysis, to food science. This is the first book on the subject and provides the reader with a fundamental introduction. Edited by a recognised authority on bijels, the reader will learn about the bijel and its formation. Bringing together current understanding, this book aims to bring the potential application of bijels to diverse materials challenges closer to fruition. This is a must-have resource for anyone working in soft matter and applied fields.
Download or read book Nanoparticles written by American Chemical Society. Meeting and published by . This book was released on 2008 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book summarizes recent advances in methods to synthesize, stabilize, passivate and functionalize diverse nanoparticles from metals, metal oxides, semiconductors, polymers, organics and biomolecules. A wide range of potential appplications with nanoparticles as building blocks are described.
Download or read book Characterization of the Surface Chemical Heterogeneities of Ultrasmall Fluorescent Silica Nanoparticles and Nanorings Using High Performance Liquid Chromatography written by Thomas Courtney Gardinier (II) and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In contrast to small molar mass compounds and organic macromolecules, detailed structural investigation of inorganic-organic core-shell nanoparticles remains a challenge. I developed high performance liquid chromatography (HPLC) methods to probe the surface chemistry of Cornell Prime dots (C’ dots), a class of ultrasmall (
Download or read book New Trends in Fluorescence Spectroscopy written by Bernard Valeur and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.
Download or read book Characterisation and Peparation sic of Fluorescent Magetic sic Silica Nanoparticles for Drug Delivery written by Niancen Peng and published by . This book was released on 2010 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: Magnetic silica nanoparticles with the core-shell structure were prepared by Stober method. There has been dramatic interest in the use of these particles for drug delivery in the recent years, however, little is known about their stability in aqueous environments. The aim of the current project is to examine their fate in water, to optimize their surface properties and finally to label them with a fluorescent dye for tracking purposes. Magnetic particles served as the core, and the silica shell was prepared from tetraethyl orthosilicate (TEOS). Changing the amount and the surface properties of the core, and varying the concentration of the shell or the solvent (ethanol) were conducted to prepare different types of magnetic silica nanoparticles. Different methods were used to characterize the nanoparticles, with the aim of finding the best conditions to prepare stable, uniform and narrow small sized nanoparticles. These measurements included: dynamic light scattering (DLS) for measuring the particles size, average count rate and to perform dissolution studies, differential scanning calorimetry (DSC) for thermal analysis of the dissolution process, UV/Vis Spectroscopy to study the turbidity, transmission electron microscopy (TEM), and measurement for zeta potential and pH. -- The first parameter studied was the magnetic core (Fe304). Coating these particles with citric acid seemed to improve the silica coating. This was attributed to the complexation between the TEOS and the citric acid, which resulted in uniform coating with silica. Another factor studied was the concentration of the precursor (TEOS). The results showed that the particle size increased with increasing the amount of TEOS. The latter was also accompanied by better stability (against dissolution) in water.
Download or read book Metal Enhanced Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2010-06-22 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
Download or read book Silica Nanoparticle Platform to Generate New Molecular Probes for Fluorescence Lifetime Imaging written by Soo Young Song and published by . This book was released on 2016 with total page 39 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence microscopy is the most widely used detection method to image molecular probes, but is currently hindered due to the limited number of fluorescent probes available. The fluorescence lifetime of fluorescent probes that is the time an excited fluorophore takes to return to the ground state; this thesis studied a novel system to manipulate the fluorescent molecules' lifetime using the nanoprobe format. This system can exponentially increase the number of fluorescent probes. The first part of this thesis reports ̃130nm silica nanoparticles labeled with silanized fluorescent dyes -- Oregon Green 488, BODIPY, and rhodamine -- using various total dye loadings. As the total dye loading increased, the relative fluorescence efficiency decreased due to self-quenching. This self-quenching effect was characterized by the phasor approach to fluorescence lifetime that gave unique and distinguishable spatial values of the nanoparticles. Utilizing the synthetic versatile properties of silica in accommodating other materials, the second part of this thesis discussed core-shell structured silica nanoparticles with an independent control to the fluorescence lifetimes of the cores and the shells. 35nm Silica cores were synthesized using a reverse microemulsion method. Various concentrations of fluorescent nanomaterials encapsulated inside the cores govern the cores' phasor values. Subsequently, silica shells with various thicknesses were synthesized using the Stober sol-gel reaction. Various total dye loadings govern the shells' phasor values while the shell thicknesses control their fluorescence intensity. The distinct fluorescence lifetime phasor values enable nanoprobes to have visually effective representations that can be easily expanded by simply manipulating the molecular proximities between fluorophores.
Download or read book Silver Micro Nanoparticles written by Samir Kumar and published by BoD – Books on Demand. This book was released on 2021-09-15 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the different methodologies for producing and synthesizing silver nanoparticles (AgNPs) of various shapes and sizes. It also provides an in-depth understanding of the new methods for characterizing and modifying the properties of AgNPs as well as their properties and applications in various fields. This book is a useful resource for a wide range of readers, including scientists, engineers, doctoral and postdoctoral fellows, and scientific professionals working in specialized fields such as medicine, nanotechnology, spectroscopy, analytical chemistry diagnostics, and plasmonics.
Download or read book Synthesis Characterization of a New Class Thiophene Containing Imidazoles as Fluorescent and Nonlinear Optical Materials written by Javier Santos-Perez and published by . This book was released on 2004 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluorescent Nanodiamonds written by Huan-Cheng Chang and published by John Wiley & Sons. This book was released on 2018-11-12 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most comprehensive reference on fluorescent nanodiamond physical and chemical properties and contemporary applications Fluorescent nanodiamonds (FNDs) have drawn a great deal of attention over the past several years, and their applications and development potential are proving to be manifold and vast. The first and only book of its kind, Fluorescent Nanodiamonds is a comprehensive guide to the basic science and technical information needed to fully understand the fundamentals of FNDs and their potential applications across an array of domains. In demonstrating the importance of FNDs in biological applications, the authors bring together all relevant chemistry, physics, materials science and biology. Nanodiamonds are produced by powerful cataclysmic events such as explosions, volcanic eruptions and meteorite impacts. They also can be created in the lab by high-pressure high-temperature treatment of graphite or detonating an explosive in a reactor vessel. A single imperfection can give a nanodiamond a specific, isolated color center which allows it to function as a single, trapped atom. Much smaller than the thickness of a human hair, a nanodiamond can have a huge surface area that allows it to bond with a variety of other materials. Because of their non-toxicity, nanodiamonds may be useful in biomedical applications, such as drug delivery and gene therapy. The most comprehensive reference on a topic of rapidly increasing interest among academic and industrial researchers across an array of fields Includes numerous case studies and practical examples from many areas of research and industrial applications, as well as fascinating and instructive historical perspectives Each chapter addresses, in-depth, a single integral topic including the fundamental properties, synthesis, mechanisms and functionalisation of FNDs The first book published by the key patent holder with his research group in the field of FNDs Fluorescent Nanodiamonds is an important working resource for a broad range of scientists and engineers in industry and academia. It will also be a welcome reference for instructors in chemistry, physics, materials science, biology and related fields.
Download or read book Semiconductor Nanomaterials written by Challa S. S. R. Kumar and published by John Wiley & Sons. This book was released on 2010-04-05 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book series Nanomaterials for the Life Sciences, provides an in-depth overview of all nanomaterial types and their uses in the life sciences. Each volume is dedicated to a specific material class and covers fundamentals, synthesis and characterization strategies, structure-property relationships and biomedical applications. The series brings nanomaterials to the Life Scientists and life science to the Materials Scientists so that synergies are seen and developed to the fullest. Written by international experts of various facets of this exciting field of research, the series is aimed at scientists of the following disciplines: biology, chemistry, materials science, physics, bioengineering, and medicine, together with cell biology, biomedical engineering, pharmaceutical chemistry, and toxicology, both in academia and fundamental research as well as in pharmaceutical companies. VOLUME 6 - Semiconductor Nanomaterials
