Download or read book Synthesis and Characterization of New Probes for Use in Fluorescence and X ray CT Bioimaging written by Simon Tang and published by . This book was released on 2015 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt: The pursuit of more suitable drugs intended for possible biological applications are a continuously growing topic of research within the scientific community. One of these suitable qualities includes the need for hydrophilicity and or some appropriate delivery system for the drug to enter into biological systems. A system of analyzing and following these compounds would then, however, be necessary to conduct any kind of mechanistic or interaction studies for the said drug within the biological system. Just to name a few, fluorescence and X-ray computed tomography (CT) methods allow for imaging of biological systems but require the need of compounds with specific qualities. Finally, even with a means of entering and following a loaded drug, it would not be complete without a way of targeting its intended location. Herein, the first chapter reports the synthesis and characterization of a fluorene-based pyridil bis-[beta]-diketone compound with suitable one- and two-photon fluorescent properties and its encapsulation into Pluronic F127 micelles for the possible application of tracking lysosomes. Next the synthesis and characterization of a BODIPY-based fluorophore with excellent fluorescence ability is reported. This compound was conjugated to two triphenylphosphine (TPP) groups and is shown as a potential mitochondria probe within HCT-116 cells. Finally, the synthesis and characterization of diatrizoic acid (DA) based derivatives conjugated to silica nanoparticles, as well as unconjugated, are reported as potential CT contrast agents. The derivatives were also functionalized with maleimide moieties facilitating subsequent potential bioconjugation of a targeting protein via a thiol group.
Download or read book Synthesis and Characterization of New Light Emitting Probes for Sensitive Detection of Bio molecules and Live Cells written by Shyamala Pravin Pillai and published by . This book was released on 2013 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: A variety of contemporary analytical platforms in technical and biological applications take advantage of labeling the objects of interest with fluorescent or luminescent tracers. Luminescent tracers take advantage of the unique property of some lanthanide metals to absorb and emit light. Long lifetime of lanthanide emission allows temporal gating of the signal, which avoids the short-lived background of interfering sample components. This property in combination with large Stokes shift contributes to extreme sensitivity of detection (ca. 10−13-10−14 M), which makes lanthanide-based probes suitable for large variety of challenging tasks ( e.g., intracellular detection of single DNA/RNA, or protein molecules, microbial pathogen detection in human specimens, tracing analysis, etc.). Luminescent probes include antenna fluorophore that absorbs light and transfers the excitation energy to a lanthanide metal tethered to antenna through chelation. The probe also contains crosslinking group that allows covalent labeling of the molecule of interest. Despite great potentials of lanthanide-based tracers the wide spread of the technology is impeded by very high price of commercially available probes to their complex structure. The goal is to develop novel approaches for the synthesis of lanthanide probes with improved quantum efficiency. This work discusses development of new strategies for synthesis of antena-fluorophores, development of new methods for introduction of the crosslinking groups in the luminescent probes and elucidates the mechanisms of chemical reactions leading to principal synthetic intermediates. New quinoline and quinolone based fluorescent compounds were synthesized, whose light emission can be conveniently tuned by simple structural modifications. Developed probes represent high-quantum yield, large Stokes shift fluorophores with amine-reactive and click-reactive groups convenient for conjugation. Some of these compounds can be used as sensitizers for lanthanide emission in design of highly sensitive luminescent probes. Obtained probes demonstrate efficient derivatization reactions allowing introduction of amine- or click-reactive crosslinking groups into the fluorophores. The reactivity of synthesized compounds is confirmed in reaction with low molecular weight nucleophiles as well as with click-reactive DNA-oligonucleotide counterparts. These reactive derivatives can be used for covalent attachment of the fluorophores to various biomolecules of interest including nucleic acids, proteins, live cells and small cellular metabolites. Synthesized compounds were characterized using N MR, steady-state and time-resolved fluorescence spectroscopy, as well UV absorption spectroscopy. This work also discusses the development of fluorescent derivatives of the antifungal drugs, posaconazole and caspofungin, for diagnostic imaging of fungal cells. Invasive fungal infections (IFI's) are a growing threat to human health particularly, with increase in the number of immune-compromised patient population, such as organ transplant recipients, al logeneic B MT, hematologic cancers; AIDS etc. The diagnosis of fungal infections is a complicated task and requires a combination of clinical observations, laboratory investigation, and radiological or other diagnostic imaging methods. Only 25% of I FI cases are diagnosed pre-mortem due to the current diagnostic challenges, which justifies the development of express diagnostic procedures. To address the issue fluorescent derivatives of the antifungal drugs, posaconazole and caspofungin, were synthesized. The fluorescent derivatives retained strong and highly specific binding to their cellular targets rendering the cells fluorescent. This new affinity-based approach strongly facilitates the detection of fungal pathogen thereby overcoming the current diagnostic challenges and can be used for clinical diagnostics. The power of this approach is not limited to fungal pathogens, but in fact represents a broader platform useful for detection of other classes of infections, both in the biological specimens and in the whole body using contemporary 3-D imaging approaches like fluorescence based in vivo imaging, luminescence imaging, positron emission tomography approach, and computer-assisted X-ray tomography.
Download or read book Design Synthesis and Characterization of New Fluorescent Probes for in Vivo Redox Visualization written by Paul R. Oleynik and published by . This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Design Synthesis and Characterization of New Two photon Absorbing 2PA Fluorescent Dyes and Bioconjugates and Their Applications in Bioimaging written by Carolina D. Andrade and published by . This book was released on 2010 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of new multiphoton absorbing materials has attracted the attention of researchers for the last two decades. The advantages that multiphoton absorbing materials offer, versus their one-photon absorbing counterparts, rely on the nature of the nonlinearity of the absorption process, where two photons are absorbed simultaneously offering increased 3D resolution, deeper penetration, and less photobleaching and photodamage as a result of a more confined excitation. The applications of efficient two-photon absorbing materials have been extensively expanding into the fields of photodynamic therapy, microscopy, and optical data storage. One of the fields where an increased interest in multiphoton absorbing materials has been most evident is in bioimaging, in particular, when different cellular processes and organelles need to be studied by fluorescence microscopy. The goal of this research was to develop efficient two-photon absorption (2PA) compounds to be used in fluorescence bioimaging, meaning that such compounds need to posses good optical properties, such as high fluorescence quantum yield, 2PA cross section, and photostability. In the first chapter of this dissertation, we describe the synthesis and structural characterization of a new series of fluorescent donor-acceptor and acceptor-acceptor molecules based on the fluorenyl ring system that incorporated functionalities such as alkynes and thiophene rings, through efficient Pd-catalyzed Sonogashira and Stille coupling reactions, in order to increase the length of the conjugation in our systems. These new molecules proved to have high two-photon absorption (2PA), and the effect of these functionalities on their 2PA cross section values was evaluated. Finally, their use in two-photon fluorescence microscopy (2PFM) imaging was demonstrated. One of the limitations of the compounds described in Chapter 1 was their poor water solubility; this issue was addressed in Chapter 2. The use of micelles in drug delivery has been shown to be an area of increasing interest over the last decade. In the bioimaging field, it is key to have dye molecules with a high degree of water solubility to enable cells to uptake the dye. By enclosing a hydrophobic dye in Pluronic® F-127 micelles, we developed a system that facilitates the use of 2PA molecules (typically hydrophobic) in biological systems for nonlinear biophotonic applications, specifically to image the lysosomes. Furthermore, we report in this chapter the efficient microwave-assisted synthesis of the dye used in this study. In addition, linear photophysical and photochemical parameters, two-photon absorption (2PA), and superfluorescence properties of the dye studied in Chapter 2, were investigated in Chapter 3. The steady-state absorption, fluorescence, and excitation anisotropy spectra of this dye were measured in several organic solvents and aqueous media. In Chapter 4, we describe the preparation and the use of an efficient and novel two-photon absorbing fluorescent probe conjugated to an antibody that confers selectivity towards the vascular endothelial growth factor receptor 2 (VEGFR-2) in porcine aortic endothelial cells that express this receptor (PAE-KDR). It is known that this receptor is overexpressed in certain cancer processes. Thus, targeting of this receptor will be useful to image the tumor vasculature. It was observed that when the dye was incubated with cells that do not express the receptor, no effective binding between the bioconjugate and the cells took place, resulting in very poor, nonspecific fluorescence images by both one and two-photon excitation. On the other hand, when the dye was incubated with cells that expressed VEGFR-2, efficient imaging of the cells was obtained, even at very low concentrations (0.4[micrometer]). Moreover, incubation of the bioconjugate with tissue facilitated successful imaging of vasculature in mouse embryonic tissue.
Download or read book Photophysics of Organic Probes and Their Applications in Bioimaging Photodynamic Therapy written by Bosung Kim and published by . This book was released on 2015 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past several decades the phenomenon of luminescence (divided into fluorescence and phosphorescence) has received great attention in the field of biological science. This quest has motivated scientists for a variety of applications, including fluorescence imaging. Fluorescence microscopy techniques that provide unique advantages, such as high spatial resolution and superior sensitivity, have been regarded as attractive tools in biophotonics. With the progress of ultrafast laser sources, two-photon absorption (2PA), in which a molecule absorbs two photons simultaneously, has opened possibilities of using it for various applications. Two-photon fluorescence microscopy (2PFM), which affords deeper tissue penetration and excellent three-dimensional (3D) images, is now being widely employed for bioimaging. This dissertation focuses on the design, synthesis, and photophysical characterization of new fluorophores, as well as desirable applications. Chapter 1 gives an account of a brief introduction of luminescence and 2PA, as well as their utilities in biological applications. In chapter 2, a series of new BODIPY derivatives are presented along with their comprehensive linear and nonlinear characteristics. They exhibited excellent photophysical properties including large extinction coefficients, high fluorescence quantum yields, good photostability, and reasonable two-photon absorption cross sections. Two promising compounds were further evaluated as NIR fluorescent probes in one-photon and two-photon fluorescence imaging.
Download or read book Synthesis and Characterization of Fluorescence Lifetime Probe Library Constructed Using Quantum Dots and Organic Dyes in a Multicomponent Silica Shell written by Ashwin Melkote Kainkaryam and published by . This book was released on 2017 with total page 53 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the recent years, significant emphasis is dedicated towards the application of nanomaterials for targeted deliveries. Using the diverse optic properties of quantum dots and fluorescent dyes and the versatile advantages of silica shell such as being biocompatible, their ease of synthesis and their drug loading platforms, they are used in biomedical and biological applications. This project explains the synthesis and characterization of multiplexed nanoprobes which are used for cancer detection studies. The nanoprobes were synthesized with various sizes and aspect ratios, involving various fluorescent dyes with a wide range of lifetime., and with varied loading concentrations of dyes and quantum dots as cores to sandwiching dyes between a shell and a core.
Download or read book Synthesis And Studies of Bimodal Imaging Probes written by V. Priya and published by . This book was released on 2023-03-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an in-depth exploration of the exciting field of bimodal imaging probes; the book covers a broad range of topics related to the synthesis and studies of bimodal imaging probes, including fluorescence, magnetic resonance, and contrast agents. It also delves into molecular imaging, nanotechnology, in vivo and in vitro imaging, optical imaging, PET imaging, CT imaging, MRI imaging, biocompatible nanoparticles, multimodal imaging, quantum dots, gold nanoparticles, surface-enhanced Raman spectroscopy, NIR fluorescence, bioimaging, drug delivery, theranostics, biosensors, cancer imaging, neuroimaging, molecular probes, cell tracking, photoacoustic imaging, ultrasensitive detection, multifunctional imaging agents, inorganic nanoparticles, organic dyes, optical coherence tomography, single photon emission computed tomography, nanoscale imaging, molecular targeting, inorganic-organic hybrid materials, biosignaling, superparamagnetic iron oxide nanoparticles, polymeric nanoparticles, nanorods, nanoshells, and nanocages. The book begins with an introduction to the field of bimodal imaging probes, providing a detailed overview of the various imaging techniques used in this field. It then delves into the synthesis of bimodal imaging probes, discussing the different types of nanoparticles and dyes used, as well as the different strategies for their synthesis. The book also covers the various studies conducted in this field, highlighting the progress made in the development of bimodal imaging probes for different applications, including cancer imaging, drug delivery, and molecular targeting. The book is an essential resource for researchers and students interested in the field of bimodal imaging probes, providing a comprehensive overview of the latest research and techniques. It is written in an accessible style and is suitable for both beginners and experts in the field.
Download or read book Synthesis Characterization and Application of Some Covalent Fluorescent Probes for Proteins written by Earl Nelson Hudson and published by . This book was released on 1970 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Silver Micro Nanoparticles written by Samir Kumar and published by BoD – Books on Demand. This book was released on 2021-09-15 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the different methodologies for producing and synthesizing silver nanoparticles (AgNPs) of various shapes and sizes. It also provides an in-depth understanding of the new methods for characterizing and modifying the properties of AgNPs as well as their properties and applications in various fields. This book is a useful resource for a wide range of readers, including scientists, engineers, doctoral and postdoctoral fellows, and scientific professionals working in specialized fields such as medicine, nanotechnology, spectroscopy, analytical chemistry diagnostics, and plasmonics.
Download or read book Synthesis of Fluorescent Molecules and Their Applications as Viscosity Sensors Metal Ion Indicators and Near infrared Probes written by Mengyuan Wang and published by . This book was released on 2014 with total page 119 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Chapter 2, a new BAPTA (O, O'-bis־בםימןנטומשל)ופטשלומוחלשדןל־,ﬞ ,ﬞ ׳ﬞ, ׳ﬞ־פופעב בדופיד בדיה) במה �ֶ�ֳִ� (4,4־היזלץןען־4־גןעב־3ב,4ב־היבתב־ף־ימהבדומו)־גבףוה דבלדיץם ימהידבפןע, �ֶָ�ֶ־3, יף עונןעפוה. ָמוק ףשמפטופיד עןץפו קבף וםנלןשוה פן ףיםנליזש גןפט ףשמפטוףיף במה נץעיזידבפיןמ, קטידט פומה פן גו לןק שיולהימח במה דץםגועףןםו זןע �ֶָ� ָהועיצבפיצוף. �נןמ ורדיפבפיןמ, ב 1.5־זןלה ימדעובףו ימ זלץןעוףדומדו ימפומףיפש ימ גץזזוע דןמפבימימח 39 [םץ]� בֵ2+ במה ב 3־זןלה ימדעובףו ימ זלץןעוףדומדו ימפומףיפש ימ גץזזוע דןמפבימימח 1 ׁבֵ2+ קבף ןגףועצוה; םןהוףפ גץפ נעןםיףימח זלץןעוףדומדו פץעמ־ןמ ומטבמדוםומפף. מֳ טֵבנפוע 3, ב מוקלש־הוףיחמוה ץמףשםםופעידבל ףסץבעבימו השו, ��3, קבף ףשמפטוףיתוה. ָןמו־נןפ ףשמפטוףיף קבף וםנלןשוה עוףץלפימח ימ ב 10% שיולה, ב עוףץלפ פטבפ יף חומועבללש סץיפו זבצןעבגלו זןע פטו דעובפיןמ ןז ץמףשםםופעידבל ףסץבעבימוף �טןפןנטשףידבל במה נטןפןדטוםידבל דטבעבדפועיתבפיןמ קבף דןמהץדפוה ימ צבעיןץף ףןלצומפף, במה ב 678 מם בגףןענפיןמ םבריםץם במה ב 692 מם וםיףףיןמ םבריםץם קועו עודןעהוה ימ �ִׁ� ףןלץפיןמ קיפט ב זלץןעוףדומדו סץבמפץם שיולה ןז 0.32. מֳ ציפען דולל ףפץהיוף הוםןמףפעבפוה פטבפ ��3 דבמ גו ץףוה בף ב מובע־�ֳ נעןגו זןע גיןיםבחימח.
Download or read book Lipid Domains written by and published by Academic Press. This book was released on 2015-06-08 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Topics in Membranes is targeted toward scientists and researchers in biochemistry and molecular and cellular biology, providing the necessary membrane research to assist them in discovering the current state of a particular field and in learning where that field is heading. This volume offers an up to date presentation of current knowledge in the field of Lipid Domains. Written by leading experts Contains original material, both textual and illustrative, that should become a very relevant reference material The material is presented in a very comprehensive manner Both researchers in the field and general readers should find relevant and up-to-date information
Download or read book Quantum Dots written by Charles Z. Hotz and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine as well as enabling applications. Part 3 shows early efforts aimed at using QDs in live animals. The final two parts demonstrate the versatility of QD technology in existing assay technology.
Download or read book Luminescent Metal Nanoclusters written by Sabu Thomas and published by Woodhead Publishing. This book was released on 2022-06-28 with total page 728 pages. Available in PDF, EPUB and Kindle. Book excerpt: Luminescent Metal Nanoclusters: Synthesis, Characterization, and Applications provides a comprehensive accounting of various protocols used for the synthesis of metal nanoclusters, their characterization techniques, toxicity evaluation and various applications and future prospects. The book provides detailed experimental routes, along with mechanisms on the formation of benign metallic clusters using biomaterials and a comprehensive review regarding the preparation, properties and prospective applications of these nano clusters in various fields, including therapeutic applications. Various methods to protect nanocluster materials to increase their stability are emphasized, including the incorporation of ligands (protein, small molecule, DNA, thiols). This book addresses a gap in the current literature by bringing together the preparation, characterization and applications of all the possible types of reported metal nanoclusters and their hybrids. It is suitable for materials scientists and engineers in academia and those working in research and development in industry. It may also be of interest to those working in the interdisciplinary nanotechnology community, such as physical chemists. Covers the most relevant material categories of luminescent nanoclusters such as metal nanoclusters, nano composites and alloy nanoclusters Provides a comprehensive overview of the various available methods used for the protection of nanoclusters Discusses the latest advances and future opportunities in addressing challenges in producing benign nanomaterials such as toxicity and stability
Download or read book Magnetic Nanomaterials written by Uyiosa Osagie Aigbe and published by Springer Nature. This book was released on 2023-08-19 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores some of the latest and recent advances in the synthesis, characterization and applications of magnetic nanomaterials. It starts with an overview of magnetic nanomaterials, followed by a list of their synthesis and characterization methods. The book shows the potential of magnetic materials in different applications, including theranostic nanomedicine, heavy metals detection, dyes sensing, solar cells, wastewater treatment, decontamination of soil, and detection and monitoring of toxic gases. Moreover, it explores their use as drug and gene delivery agents, their biosafety and bioregulation facets, tissue engineering applications, and their potential for combating pathogens
Download or read book Particulate Systems in Nano and Biotechnologies written by Wolfgang Sigmund and published by CRC Press. This book was released on 2008-12-22 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite the widespread growth and acceptance of particulate technology, challenges in the design, operation, and manufacturing of these systems still exists. These critical issues must be resolved so that particle technology may continue to serve as a foundation for new nano and biotechnologies. Particulate Systems in Nano- and Biotechnologies pres
Download or read book State of the Art in Nano bioimaging written by Morteza Sasani Ghamsari and published by BoD – Books on Demand. This book was released on 2018-06-20 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nano-bioimaging is a real-time observation method for the study of biological processes in subcellular structures and entire cells. This technique aims to interfere as little as possible with life processes using nanoscale materials and probes. In this method, nanoscale photon source is often used for imaging, and 3D structure of the observed specimen is studied in detail without physical interference. Over the last decade, further boost in bioimaging has led to increase the nano-bioimaging impact that includes many improvements in the data analysis method, image processing, and molecular imaging technology. However, to increase the usage of nano-bioimaging, several developments in the field of diagnosis accuracy, photobleaching prevention, and controlling of the fluorescence resonance energy transfer (FRET) must be achieved. The purpose of this book is to provide a perspective on the current status of nano-bioimaging technologies.
Download or read book Metal Enhanced Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2010-06-22 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
