Download or read book Synthesis and Characterization of Molecular Rotors as Viscosity Sensors and Beta Amyloid Binding Agents written by Jeyanthy Sutharsan and published by . This book was released on 2009 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been shown that compounds containing p-N, N, -dialkylaminobezylidene cyanoacetate motif can serve as fluorescent non-mechanical viscosity sensors. These compounds, referred to as molecular rotors, belong to a class of fluorescent probes that are known to form twisted intra-molecular charge-transfer complexes in the excited state. The first part of this work deals with the synthesis and evaluation of these compounds as viscosity sensors. The effects of the molecular structure and electronic density of these rotors to the emission wavelength, fluorescence intensity and viscosity sensitivity are presented. The second part of this work deals with the evaluation of molecular rotors as beta amyloid binding agents. The findings suggest that the general motif of molecular rotors can be applied to a rational design of new diagnostic tools for beta amyloid imaging in Alzheimer's disease.
Download or read book Rational Design of Molecular Rotors as Beta Amyloid Binding Agents and Viscosity Sensors written by Willy M. Chang and published by . This book was released on 2011 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: A new family of fluorescent markers, also known as molecular rotors, containing an Amino Naphthalenyl-2-Cyano-Acrylate (ANCA) motif has been synthesized and evaluated for its capability to associate with aggregated [beta]-amyloid (AP) peptides. Chemical modifications were introduced to explore their effect on the capability of the ANCA-based probes to fluorescently label aggregated A[beta] peptides in human brain tissue from patients with Alzheimer's disease. The effects on AP binding affinity, fluorescence intensity, excitation and emission, and molecular property are presented. Furthermore, the evaluation of molecular probes as non-mechanical viscosity sensors is explored. The findings suggest that the molecular rotor motif can be applied towards designing promising new diagnostic tools for measuring viscosity in biological systems and monitoring for disease.
Download or read book Molecular Rotors as Sensors of Microscopic Viscosity and Temperature written by Aurimas Vysniauskas and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Synthesis and Characterization of Salicylideneaniline Functionalized Molecular Rotors written by Ira Owen Staehle and published by . This book was released on 2014 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Artificial molecular machines have drawn much interest over the past twenty years. Several physical organic chemists have been actively involved in the study and development of different types of molecular machines. In nature many biological macromolecules have the ability to act as "molecular machines" and generate work. The most well known example of this is ATPsynthase, which functions by rotation of a central stock in order to convert ADP to ATP. These biological "molecular machines" are typically highly complex and consist of several subunits. However it is in this complexity that the function of these biological machines is manifested. In order to attain this level of complexity in artificial molecular machines, a gradual development of concepts and a deeper understanding of intermolecular interactions are required. Several molecular machine motifs have been identified and further functionality has been explored by many physical organic chemists, incorporating useful material properties. One way to create artificial molecular machines is by controlling non-brownian motion. A common motif is a system that has two distinct energy states that can interconvert between one another in response to an external stimulus. These types of molecular machine motifs can be used for generating materials that are capable of energy harnessing. Research in the Garcia-Garibay group has focused on the use of light as an external stimulus to facilitate a conformational change between two distinct states in a molecular rotor. Chapter 2 of this thesis will discuss the functionalization of molecular rotors with salicylideneanilines rotators. In work done in collaboration with Dr. Braulio Rodriguez-Molina, three different photoresponsive molecular rotors were synthesized, characterized, and crystallized. The photochromism of these solids was tested, with all systems converting from the low energy cis-enol to the transient trans-keto. The best performing of these three different molecular rotors was identified by the generation of the most trans-keto upon photoexcitation. A deuterated rotator analog was synthesized and the activation energy for rotation in the solid state was determined. Prior to this work, the preferred isomerization pathway for salicylidenenanilines was through a bicycle pedal mechanism, which does not account for rotation. The ssNMR data for the deuterated analog indicated that isomerization could also occur through rotation. Chapters 3 and 4 will discuss the functionalization of molecular rotors with salicylideneanilines stators to act as potential molecular brakes. In chapter 3, an amino substituted stator was synthesized and characterized. Crystallization proved challenging due to the preference of these compounds for the formation of spherulites, which are radial fibrous microcrystalline aggregate structures. Nonetheless, solid-state photochromism of the spherulites was determined for both systems by diffuse reflectance with the generation of a transient trans-keto form after photoexcitation. In chapter 4, an ester-substituted stator was synthesized and characterized. Single crystals could not be obtained and ssNMR was utilized to explore both the photochromism and thermochromism of the salicyladenanilines. In chapter 5 of this thesis, I will discuss the synthesis and characterization of tetra-substituted salicylideneaniline adamantanes. Adamantanes are unique because they are the simplest diamonds and provide a rigid framework with minimal stress. Furthermore, tetra-substitution of adamantane generates a tetrahedral geometry around the adamantane core. Two adamantane systems were synthesized and single crystals were grown. However, only the connectivity was determined, due to poor quality crystals. These generated molecules could have up to 64 isomers, depending on the conformations of the four salicylideneanilines moieties. The photochromism of these solids was then tested with both systems generating the transient trans-keto species.
Download or read book Advanced Fluorescence Reporters in Chemistry and Biology II written by Alexander P. Demchenko and published by Springer Science & Business Media. This book was released on 2010-09-08 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts
Download or read book Handbook of Photonics for Biomedical Engineering written by Aaron H.-P. Ho and published by Springer. This book was released on 2016-09-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanophotonics has emerged rapidly into technological mainstream with the advent and maturity of nanotechnology available in photonics and enabled many new exciting applications in the area of biomedical science and engineering that were unimagined even a few years ago with conventional photonic engineering techniques. Handbook of Nanophotonics in Biomedical Engineering is intended to be a reliable resource to a wealth of information on nanophotonics that can inspire readers by detailing emerging and established possibilities of nanophotonics in biomedical science and engineering applications. This comprehensive reference presents not only the basics of nanophotonics but also explores recent experimental and clinical methods used in biomedical and bioengineering research. Each peer-reviewed chapter of this book discusses fundamental aspects and materials/fabrication issues of nanophotonics, as well as applications in interfaces, cell, tissue, animal studies, and clinical engineering. The organization provides quick access to current issues and trends of nanophotonic applications in biomedical engineering. All students and professionals in applied sciences, materials, biomedical engineering, and medical and healthcare industry will find this essential reference book highly useful.
Download or read book Linear Dichroism and Circular Dichroism written by Bengt Nordén and published by Royal Society of Chemistry. This book was released on 2019-05-02 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an introduction to optical anisotropy (linear dichroism, LD) and optical activity (circular dichroism, CD) as techniques for the study of structures and interactions of molecules in solution. The book covers the use of these techniques for both small and large molecular systems with particular emphasis being placed on proteins and nucleic acids. CD is a well-established technique and this book aims to explain how it can be used simply and effectively for new entrants to the field as well as covering more advanced techniques for experts. LD is often seen as a rather exotic method intended only for experienced spectroscopists. This book demonstrates that it is an approach with real utility that may be used by both students and scientists from graduate level onwards to give simple answers, which are not available from any other technique, to structural and kinetic questions. Much of the emphasis is on flow orientation of samples in solution phase. The book first describes the techniques and the information they can provide; it then goes on to give specific details on how to actually implement them, including a wide range of examples showing how LD and CD can help with * protein and nucleic acid secondary structure elucidation; * analysis of the formation and rearrangements of fibrous proteins and membrane proteins; * identification of the absolute configuration of small molecules; * determination of the orientation of small molecules in anisotropic media; * assignment of transition moment polarizations; * investigation of binding strengths and geometries of ligand-macromolecule complexes; * 3-D structure determination from LD, molecular replacement and MD modeling. The advantages of combined LD/CD studies are also outlined with examples of DNA/drug complexes and protein insertion into membranes. Taken together the book represents a comprehensive text on the theory and application of LD and CD in the chemical and biological sciences.
Download or read book Protein Self Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Download or read book The Perfect Slime written by Hans-Curt Flemming and published by IWA Publishing. This book was released on 2016-09-15 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Perfect Slime presents the latest state of knowledge and all aspects of the Extracellular Polymeric Substances, (EPS) matrix – from the ecological and health to the antifouling perspectives. The book brings together all the current material in order to expand our understanding of the functions, properties and characteristics of the matrix as well as the possibilities to strengthen or weaken it. The EPS matrix represents the immediate environment in which biofilm organisms live. From their point of view, this matrix has paramount advantages. It allows them to stay together for extended periods and form synergistic microconsortia, it retains extracellular enzymes and turns the matrix into an external digestion system and it is a universal recycling yard, it protects them against desiccation, it allows for intense communication and represents a huge genetic archive. They can remodel their matrix, break free and eventually, they can use it as a nutrient source. The EPS matrix can be considered as one of the emergent properties of biofilms and are a major reason for the success of this form of life. Nevertheless, they have been termed the “black matter of biofilms” for good reasons. First of all: the isolation methods define the results. In most cases, only water soluble EPS components are investigated; insoluble ones such as cellulose or amyloids are much less included. In particular in environmental biofilms with many species, it is difficult to impossible isolate, separate the various EPS molecules they are encased in and to define which species produced which EPS. The regulation and the factors which trigger or inhibit EPS production are still very poorly understood. Furthermore: bacteria are not the only microorganisms to produce EPS. Archaea, Fungi and algae can also form EPS. This book investigates the questions, What is their composition, function, dynamics and regulation? What do they all have in common?
Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.
Download or read book Experimental Approaches of NMR Spectroscopy written by The Nuclear Magnetic Resonance Society of Japan and published by Springer. This book was released on 2017-11-23 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the advanced developments in methodology and applications of NMR spectroscopy to life science and materials science. Experts who are leaders in the development of new methods and applications of life and material sciences have contributed an exciting range of topics that cover recent advances in structural determination of biological and material molecules, dynamic aspects of biological and material molecules, and development of novel NMR techniques, including resolution and sensitivity enhancement. First, this book particularly emphasizes the experimental details for new researchers to use NMR spectroscopy and pick up the potentials of NMR spectroscopy. Second, the book is designed for those who are involved in either developing the technique or expanding the NMR application fields by applying them to specific samples. Third, the Nuclear Magnetic Resonance Society of Japan has organized this book not only for NMR members of Japan but also for readers worldwide who are interested in using NMR spectroscopy extensively.
Download or read book Fragment based Approaches in Drug Discovery written by Wolfgang Jahnke and published by John Wiley & Sons. This book was released on 2006-12-13 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first systematic summary of the impact of fragment-based approaches on the drug development process provides essential information that was previously unavailable. Adopting a practice-oriented approach, this represents a book by professionals for professionals, tailor-made for drug developers in the pharma and biotech sector who need to keep up-to-date on the latest technologies and strategies in pharmaceutical ligand design. The book is clearly divided into three sections on ligand design, spectroscopic techniques, and screening and drug discovery, backed by numerous case studies.
Download or read book Molecular Architectonics and Nanoarchitectonics written by Thimmaiah Govindaraju and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the ultimate assembly of recent research activities on molecular architectonics and nanoarchitectonics by authors who are worldwide experts. The book proposes new ways of creating functional materials at the nano level using the concepts of molecular architectonics and nanoarchitectonics, which are expected to be the next-generation approaches beyond conventional nanotechnology. All the contents are categorized by types of materials, organic materials, biomaterials, and nanomaterials. For that reason, non-specialists including graduate and undergraduate students can start reading the book from any points they would like. Cutting-edge trends in nanotechnology and material sciences are easily visible in the contents of the book, which is highly useful for both students and experimental materials scientists. .
Download or read book Femtochemistry written by Frans C. De Schryver and published by Wiley-VCH. This book was released on 2001-10-25 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: For decades now, chemists have been dreaming of observing exactly what happens at the molecular level during a chemical reaction. The breakthrough came at the end of the 80s with the development of special spectrometers that use extremely short-pulsed laser light and so enable measurements in the femtosecond range. Since then, "femtochemistry" has made rapid progress, due to intensive collaboration between physical chemists, spectroscopists, photochemists and physicists. The first-class contributions collected here from top international femtochemists provide a comprehensive insight into this area of interdisciplinary research. The range extends from femtochemistry in nanohollows to the investigation of the dynamics of biological reactions in the femtosecond range, from ab initio approaches to quantum chemical reaction controls to two-dimensional, nonlinear optical spectroscopy in liquids. This overview of the current level of research is rounded off with A. Zewail's Nobel prizewinning lecture. The vast amount of information and numerous references make this not only an ideal introduction to the research, but also recommended reading for everyone concerned with this hot topic.
Download or read book Nanomaterials Chemistry written by C. N. R. Rao and published by John Wiley & Sons. This book was released on 2007-09-24 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: With this handbook, the distinguished team of editors has combined the expertise of leading nanomaterials scientists to provide the latest overview of this field. They cover the whole spectrum of nanomaterials, ranging from theory, synthesis, properties, characterization to application, including such new developments as quantum dots, nanoparticles, nanoporous materials, nanowires, nanotubes, and nanostructured polymers. The result is recommended reading for everybody working in nanoscience: Newcomers to the field can acquaint themselves with this exciting subject, while specialists will find answers to all their questions as well as helpful suggestions for further research.
Download or read book Polymer Colloids written by Rodney Priestley and published by Royal Society of Chemistry. This book was released on 2019-12-02 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Academic and industrial research around polymer-based colloids is huge, driven both by the development of mature technologies, e.g. latexes for coatings, as well as the advancement of new materials and applications, such as building blocks for 2D/3D structures and medicine. Edited by two world-renowned leaders in polymer science and engineering, this is a fundamental text for the field. Based on a specialised course by the editors, this book provides the reader with an invaluable single source of reference. The first section describes formation, explaining basic properties of emulsions and dispersion polymerization, microfluidic approaches to produce polymer-based colloids and formation via directed self-assembly. The next section details characterisation methodologies from microscopy and small angle scattering, to surface science and simulations. The final chapters close with applications, including Pickering emulsions and molecular engineering for materials development. A comprehensive guide to polymer colloids, with contributions by leaders in their respective areas, this book is a must-have for researchers and practitioners working across polymers, soft matter and chemical and molecular engineering.
Download or read book Practical Fluorescence Theory Methods and Techniques written by George G. Guilbault and published by . This book was released on 1973 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt:
