Download or read book Synthesis and Biophysical Characterization of Cationic Amphipathic PH Responsive Gene Delivery Peptides Containing Primary Beta amine 2 3 diaminopropionic Acid Residues and Secondary Beta amine Mono methylated Derivatives written by Jayneil Patel and published by . This book was released on 2011 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Delivery of therapeutic oligonucleotide sequences by cationic amphipathic histidine rich peptides like LAH4-L1 is modulated by peptide response to pH changes during endocytosis. pH sensitivity can be affected by the number of histidine residues, defining its transfection efficiency. Optimizing the gene delivery efficiencies of LAH4-L1 by increasing the number of histidine residues and therefore peptide charge is limited by the Coulombic interactions that stabilise a-helical self-associated peptide oligomers. Consequently, peptides containing six histidine residues function at a pH below that which occurs in the endosome, decreasing gene delivery capabilities. This limitation is expected to be overcome by substituting histidine residues with the more basic pH response 2,3- diaminopropionic acid (Dap). It is predicted that peptides containing 6 Dap residues will remain sensitive to pH changes observed in the endosome, leading to more efficient gene delivery peptide. To test these hypothesis peptides containing four and six Dap residues were synthesised. Analogously, derivatives containing mono-methylated P-amine Dap residues were formed in attempt to reduce cytotoxicity associated with primary amine peptides. Biophysical analysis was performed to determine the peptides pH response in key mechanisms that occur during gene delivery. It is shown that Dap residues can balance the Coulombic effects and respond at a more basic pH than histidine containing analogues. Whereas Dap substitution does not give increased gene delivery compared to LAH4-L1; mono-methylated Dap variants remain sensitive to pH changes in endocytosis and show increased gene delivery efficiencies compared to LAH4-L1. Peptides containing six mono-methylated Dap residues gave transfection results reproducible in serum greater than LAH4-L1 and comparable to commercially available peptide vectors.

Download or read book Synthesis and Biophysical Characterisation of Cationic Amphipathic PH Responsive Gene Delivery Peptides Containing Primary amine 2 3 diaminopropionic Acid Residues and Secondary amine Mono methylated Derivatives written by Jayneil Patel and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Delivery of therapeutic oligonucleotide sequences by cationic amphipathic histidine rich peptides like LAH4-L1 is modulated by peptide response to pH changes during endocytosis. pH sensitivity can be affected by the number of histidine residues, defining its transfection efficiency. Optimizing the gene delivery efficiencies of LAH4-L1 by increasing the number of histidine residues and therefore peptide charge is limited by the Coulombic interactions that stabilise a-helical self-associated peptide oligomers. Consequently, peptides containing six histidine residues function at a pH below that which occurs in the endosome, decreasing gene delivery capabilities. This limitation is expected to be overcome by substituting histidine residues with the more basic pH response 2,3- diaminopropionic acid (Dap). It is predicted that peptides containing 6 Dap residues will remain sensitive to pH changes observed in the endosome, leading to more efficient gene delivery peptide. To test these hypothesis peptides containing four and six Dap residues were synthesised. Analogously, derivatives containing mono-methylated P-amine Dap residues were formed in attempt to reduce cytotoxicity associated with primary amine peptides. Biophysical analysis was performed to determine the peptides pH response in key mechanisms that occur during gene delivery. It is shown that Dap residues can balance the Coulombic effects and respond at a more basic pH than histidine containing analogues. Whereas Dap substitution does not give increased gene delivery compared to LAH4-L1; mono-methylated Dap variants remain sensitive to pH changes in endocytosis and show increased gene delivery efficiencies compared to LAH4-L1. Peptides containing six mono-methylated Dap residues gave transfection results reproducible in serum greater than LAH4-L1 and comparable to commercially available peptide vectors.

Download or read book Nanotechnology for Nucleic Acid Delivery written by Manfred Ogris and published by Humana Press. This book was released on 2012-10-16 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way. Nucleic acids offer the potential for highly selective treatment of such diseases or the highly specific modulation of gene expression with RNA interference. A key issue for successful nucleic acid therapies is the availability of a suitable delivery system. Here, the field of nanotechnology offers a multitude of possibilities to develop nanosized delivery vectors tailor-made for various local and systemic approaches. In Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, experts in the field cover the area of nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Download or read book Targeted Drug Strategies for Cancer and Inflammation written by Ann L. Jackman and published by Springer Science & Business Media. This book was released on 2011-05-05 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: Folate pathways are essential in metabolism and macromolecule synthesis. Antifolate drugs that are largely transported via a high capacity folate transporter (i.e. the reduced-folate carrier) and inhibit folate-dependent enzymes include the dihydrofolate reductase inhibitor, methotrexate, and the thymidylate synthase inhibitors, raltitrexed and pemetrexed. Major advances in folate research made within the last decade include (i) the approval of pemetrexed for the treatment of lung cancer and mesothelioma, and (ii) the demonstration that cell membrane-anchored folate receptors (FR) are exploitable for cancer and inflammatory disease management. FRs are not widely distributed in normal tissues, except on some luminal surfaces; however, they are accessible to systemically administered agents when expressed on many cancers as well as on activated macrophages involved in various inflammatory diseases. High affinity folate-radioisotope conjugates have been developed for imaging pathogenic FR-positive diseases, including cancer. Since the FR transports folates via a low capacity but high affinity endocytic pathway, a variety of FR-targeted antifolate drugs and folate conjugates bearing a wide range of payloads (including cytotoxic drugs) are currently being developed which exploit this property. The FR is also being utilized in immunotherapy approaches for the treatment of overexpressing cancers.

Download or read book Telomerase Inhibition written by Lucy Andrews and published by Springer Science & Business Media. This book was released on 2007-11-29 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a compendium of the most recent and advanced methods applied to the rapidly expanding field of telomerase inhibition. The techniques described provide the researcher with a diverse and comprehensive set of tools for the study of telomerase inhibition. The volume is aimed at biochemists, molecular biologists, cancer researchers, and geneticists.

Download or read book Intracellular Delivery written by Aleš Prokop and published by Springer Science & Business Media. This book was released on 2011-05-26 with total page 871 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book features a special subsection of Nanomedicine, an application of nanotechnology to achieve breakthroughs in healthcare. It exploits the improved and often novel physical, chemical and biological properties of materials only existent at the nanometer scale. As a consequence of small scale, nanosystems in most cases are efficiently uptaken by cells and appear to act at the intracellular level. Nanotechnology has the potential to improve diagnosis, treatment and follow-up of diseases, and includes targeted drug delivery and regenerative medicine; it creates new tools and methods that impact significantly upon existing conservative practices. This volume is a collection of authoritative reviews. In the introductory section we define the field (intracellular delivery). Then, the fundamental routes of nanodelivery devices, cellular uptake, types of delivery devices, particularly in terms of localized cellular delivery, both for small drug molecules, macromolecular drugs and genes; at the academic and applied levels, are covered. The following section is dedicated to enhancing delivery via special targeting motifs followed by the introduction of different types of intracellular nanodelivery devices (e.g. a brief description of their chemistry) and ways of producing these different devices. Finally, we put special emphasis on particular disease states and on other biomedical applications, whilst diagnostic and sensing issues are also included. Intracellular delivery / therapy is a highly topical which will stir great interest. Intracellular delivery enables much more efficient drug delivery since the impact (on different organelles and sites) is intracellular as the drug is not supplied externally within the blood stream. There is great potential for targeted delivery with improved localized delivery and efficacy.

Download or read book Dendritic Molecules written by George R. Newkome and published by John Wiley & Sons. This book was released on 2008-07-11 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first book on dendrimers! The authors, pioneers in this scientific field, describe basic principles and current developments in the rapidly evolving field of dendrimer research. All aspects of the subject are covered: the authors provide a historical overview, theoretical background, and discussions of the synthesis and applications of dendrimers. The book thus spans organic chemistry and more application-orientated disciplines like material science and pharmaceutical chemistry. The excellent, detailed list of references further increases the value of the book. The optimal presentation of the structural formulas of dendrimers - highly symmetrical giant molecules - helps the reader understand the sophisticated synthesis quickly. This book will set the standard for further monographs on this subject.

Download or read book Peptide and Protein Engineering written by Olga Iranzo and published by Humana. This book was released on 2021-07-23 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thorough book aims to present the methods that have enabled the success of peptides and proteins in a wide variety of applications. It opens with a section on chemical tools applied to the production or engineering of peptides and proteins, and concludes with a collection of chapters on biological approaches used to engineer structure and function in peptides and proteins. As a book in the Springer Protocols Handbooks series, chapters include the kind of detailed descriptions and tips necessary for successful results in practice. Authoritative and practical, Peptide and Protein Engineering: From Concepts to Biotechnological Applications will be of great use to scientists in academia and industry seeking a better understanding of the emerging principles and methodologies in peptide and protein engineering.

Download or read book Protein Protein Interaction Regulators written by Siddhartha Roy and published by Royal Society of Chemistry. This book was released on 2020-12-14 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: New genomic information has revealed the crucial role that protein–protein interactions (PPIs) play in regulating numerous cellular functions. Aberrant forms of these interactions are common in numerous diseases and thus PPIs have emerged as a vast class of critical drug targets. Despite the importance of PPIs in biology, it has been extremely challenging to convert targets into therapeutics and targeting PPIs had long been considered a very difficult task. However, over the past decade the field has advanced with increasing growth in the number of successful PPI regulators. Protein–Protein Interaction Regulators surveys the latest advances in the structural understanding of PPIs as well as recent developments in modulator discovery.

Download or read book Life Cycle Analysis of Nanoparticles written by Ashok Vaseashta and published by DEStech Publications, Inc. This book was released on 2015-03-30 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Investigative tools for analyzing environmental nanoparticles with health impactsBasic theories and models of life cycle analysis applied to nanomaterialsConnects LCA, detection technologies and sustainability This book addresses the ways life cycle assessment (LCA) concepts can be applied to analyze the fate of nanoparticles in a variety of environmental and manufacturing settings. After introducing LCA theory and modeling concepts, the work discusses risks associated with carbon nanotubes, graphene, silver, fullerenes, iron oxides and other particles generated by manufacturing or medical diagnostics. Chapters in the text discuss biomolecules and the application of in vivo biosensors. Also covered are fate analysis, risk assessment, toxicology and nanopathology with a focus on human health and disease.

Download or read book Peptides Across the Pacific written by Michal Lebl and published by . This book was released on 2013-09-30 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the symposium in June 2013 in Big Island, Hawaii

Download or read book Biosensors for Medical Applications written by Séamus Higson and published by Elsevier. This book was released on 2012-08-21 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical sensors are an essential tool in the detection and monitoring of a wide range of medical conditions from cancer to Parkinson’s disease. Biosensors for medical applications provides a comprehensive review of established, cutting edge and future trends in biomedical sensors and their applications. Part one focuses on key principles and transduction approaches, reviewing electrochemical, piezoelectric and nano-sized biosensors. Impedence interrogated affinity biosensors for medical applications and practical applications of enzyme biosensors are explored, before part two goes on to review specific medical applications. Biosensors for DNA and RNA detection and characterization, disease biomarker detection, and the use of affibodies as an alternative to antibodies in cancer marker biosensors are investigated, along with biosensors for drug testing and discovery, non-invasive measurements, and wearable biosensors for medical applications. With its distinguished editor and international team of expert contributors, Biosensors for medical applications is an essential guide for all those involved in the research, design, production and use of medical biosensors. Provides a comprehensive review of established, cutting edge and future trends in biomedical sensors and their applications Examines key principles and transduction approaches, reviewing electrochemical, piezoelectric and nano-sized biosensors Reviews biosensors for DNA and RNA detection and characterisation, disease biomarker detection, and the use of affibodies as an alternative to antibodies in cancer marker biosensors

Download or read book Cell Signaling in Model Plants 2 0 written by Jen-Tsung Chen and published by Mdpi AG. This book was released on 2021-10-29 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell signaling is an intensive research topic in which reductionism can be achieved when we investigate the systems of model plants. To continue our previous book entitled "Cell Signaling in Model Plants", the second volume explores more insights into the regulatory mechanisms of plant growth and development and eventually collects 20 publications that consist of one editorial, eleven original research articles and eight literature reviews. This book only presents a snapshot of this intensive field of plant biology, and we believe that with the rapid development of advanced technology such as integrative multi-omics and CRISPR genome editing, more and more critical networks of plant cell signaling will be unraveled in the future.

Download or read book Spectroscopic Methods in Bioinorganic Chemistry written by Edward I. Solomon and published by . This book was released on 1998 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains recent advances in spectrographic methods, including EPR, magnetic Mossbauer, paramagnetic and multi-D NMR, metalloprotein crystallography, EAS, magnetic circular dichroism, resonance Raman, X-ray absorption spectroscopy, and electron structure calculations. The book concentrates on topics where spectrographic methods have had a major impact, such as electron transfer, cluster interactions, intermediates, and definition of active site structure, and it includes a thorough tutorial on basic methods.

Download or read book Chemical Approaches to the Synthesis of Peptides and Proteins written by Paul Lloyd-Williams and published by CRC Press. This book was released on 1997-04-23 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: Organic chemists working on the synthesis of natural products have long found a special challenge in the preparation of peptides and proteins. However, more reliable, more efficient synthetic preparation methods have been developed in recent years. This reference evaluates the most important synthesis methods available today, and also considers methods that show promise for future applications. This text describes the state of the art in efficient synthetic methods for the synthesis of both natural and artificial large peptide and protein molecules. Subjects include an introduction to basic topics, linear solid-phase synthesis of peptides, peptide synthesis in solution, convergent solid-phase synthesis, methods for the synthesis of branched peptides, formation of disulfide bridges, and more. The book emphasizes strategies and tactics that must be considered for the successful synthesis of peptides.

Download or read book Dynamical Tunneling written by Srihari Keshavamurthy and published by CRC Press. This book was released on 2011-03-09 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: A prominent aspect of quantum theory, tunneling arises in a variety of contexts across several fields of study, including nuclear, atomic, molecular, and optical physics and has led to technologically relevant applications in mesoscopic science. Exploring mechanisms and consequences, Dynamical Tunneling: Theory and Experiment presents the work of i

Download or read book Peptide Based Materials written by Timothy Deming and published by Springer Science & Business Media. This book was released on 2012-01-10 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthesis of Polypeptides by Ring-Opening Polymerization of α-Amino Acid N-Carboxyanhydrides, by Jianjun Cheng and Timothy J. Deming.- Peptide Synthesis and Self-Assembly, by S. Maude, L. R. Tai, R. P. W. Davies, B. Liu, S. A. Harris, P. J. Kocienski and A. Aggeli.- Elastomeric Polypeptides, by Mark B. van Eldijk, Christopher L. McGann, Kristi L. Kiick andJan C. M. van Hest.- Self-Assembled Polypeptide and Polypeptide Hybrid Vesicles: From Synthesis to Application, by Uh-Joo Choe, Victor Z. Sun, James-Kevin Y. Tan and Daniel T. Kamei.- Peptide-Based and Polypeptide-Based Hydrogels for Drug Delivery and Tissue Engineering, by Aysegul Altunbas and Darrin J. Pochan.-