Download or read book Surface Enhanced Raman Scattering Based Assays for DNA Detection by written by Danny van Lierop and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Surface enhanced Raman Scattering Based Biosensor Platforms for Viral DNA Detection written by Hao Zhang and published by . This book was released on 2011 with total page 133 pages. Available in PDF, EPUB and Kindle. Book excerpt: SERS based DNA biosensors have recently gained considerable attention for detection of target gene sequences related to diseases in medicine, the food industry, the environment and agriculture. In this work, we developed three nanoparticle based SERS assays for detection of viral DNA. First, an indirect capture model assay using Au nanoparticles was demonstrated for SERS detection of DNA derived from the West Nile Virus (WNV) RNA genome. Upon incubation with colloidal Au, hybridization complexes containing the WNV target sequence, a complementary capture oligonucleotide conjugated to a strong tethering group and a complementary reporter oligonucleotide conjugated to methylene blue (MB), a Raman label, anchors the resultant ternary complex to Au nanoparticles and positions MB within the required sensing distance for SERS enhancement. The SERS test provides a spectral peak signature profile that is capture-specific and characteristic of the Raman spectrum for MB. The next study decribes a model paramagnetic nanoparticle (MNP) assay that is demonstrated for SERS detection of WNV target DNA sequence. Detection is based on the capture of WNV target sequences by hybridization with complementary oligonucleotide probes covalently linked to fabricated MNPs and Raman reporter tag-conjugated gold nanoparticles (GNPs) and the subsequent removal of GNP-WNV target sequence-MNP hybridization complexes from solution by an externally applied magnetic source. Laser excitation of the pelleted material provided a signature SERS spectrum which is diagnostic for the reporter, 5,5 feet-dithiobis(succinimidy-2-nitrobenzoate) (DSNB), and restricted to hybridization reactions containing WNV target sequences. Finally, we developed a magnetic capture-based SERS assay for the viral DNA multiplex detection in solution using Au-coated paramagnetic nanoparticles as SERS-active capture substrates and Raman tag-conjugated oligos as reporter probes. Using the DNA derived from the Rift Valley Fever Virus (RVFV) and West Nile Virus (WNV) RNA genome, WNV as the model targets, monoplex and multiplex SERS detection were designed and demonstrated.
Download or read book DNA Diagnostic Assays Using Surface Enhanced Raman Scattering SERS written by Mhairi Harper and published by . This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA is the prerequisite for all biological life and its discovery has revolutionised the understanding of biomolecular interactions and disease expression. This has enabled significant improvements in patient diagnosis and medical treatment to be carried out. The advancements in technology and instrumentation have continually progressed this knowledge and continue to push the boundaries of diagnostic and clinical advancements. One effective way to achieve this is through application of dye labelled DNA sequences and metallic nanoparticle suspensions. This research details an understanding of the interaction between dye labelled oligonucleotides and silver nanoparticle surfaces, which generate strong surface enhanced Raman scattering (SERS) responses through specific hybridisation events which correlate to the presence of targeted sequences. During this study, the attraction of oligonucleotides onto metal nanoparticles was shown to be driven through the DNA nucleobases. Therefore, the increased exposure of the base groups within single stranded DNA sequences generated a higher affinity for metal surfaces which in turn produced stronger SERS responses when compared to double stranded DNA. This principle was utilised within a DNA detection assay to successfully demonstrate the presence of target DNA sequences. Two novel DNA detection assays were also investigated which utilised SERS to determine the presence of sequences relating to the methicillin resistant Staphylococcus aureus (MRSA) strain. A solution based detection method was developed through coupling a TaqMan assay with SERS. This combination enabled highly specific detection of clinically relevant sequences of MRSA to be obtained with 7 fM limits of detection achievable. The multiple detection of different genomic S. aureus strains was achieved through the molecularly specific and narrow emission spectral profiles obtained. A contrasting DNA detection strategy which relies upon the hybridisation of complementary sequences on a solid substrate surface was shown. Silver nanoparticles were functionalised with specific DNA sequences and a variety of SERS active molecules, enabling the selective detection of target sequences from nitrocellulose membranes. This thesis has exploited SERS to enable the specific identification of DNA sequences to be achieved via utilisation of silver nanoparticles. Through SERS, an insight into the interactions of DNA and silver nanoparticles surfaces has been gained as well as enhancing the sensitivity and specificity achievable within SERS detection assays.
Download or read book Surface Enhanced Raman Spectroscopy written by Marek Prochazka and published by Springer. This book was released on 2015-12-12 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives an overview of recent developments in RS and SERS for sensing and biosensing considering also limitations, possibilities and prospects of this technique. Raman scattering (RS) is a widely used vibrational technique providing highly specific molecular spectral patterns. A severe limitation for the application of this spectroscopic technique lies in the low cross section of RS. Surface-enhanced Raman scattering (SERS) spectroscopy overcomes this problem by 6-11 orders of magnitude enhancement compared with the standard RS for molecules in the close vicinity of certain rough metal surfaces. Thus, SERS combines molecular fingerprint specificity with potential single-molecule sensitivity. Due to the recent development of new SERS-active substrates, labeling and derivatization chemistry as well as new instrumentations, SERS became a very promising tool for many varied applications, including bioanalytical studies and sensing. Both intrinsic and extrinsic SERS biosensing schemes have been employed to detect and identify small molecules, nucleic acids and proteins, and also for cellular and in vivo sensing.
Download or read book Surface Enhanced Raman Scattering Detection of DNA Assemblies in Solution written by Michael Bellavia and published by . This book was released on 2013 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Surface Plasmon Resonance written by Nico J. de Mol and published by Humana Press. This book was released on 2011-08-24 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, experts in the field present a wide variety of applications involving commercially available SPR instruments. The book fills a need for well-described, hands-on SPR experimental protocols, and it highlights the backgrounds of vital issues.
Download or read book Principles of Surface Enhanced Raman Spectroscopy written by Eric Le Ru and published by Elsevier. This book was released on 2008-11-17 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt: SERS was discovered in the 1970s and has since grown enormously in breadth, depth, and understanding. One of the major characteristics of SERS is its interdisciplinary nature: it lies at the boundary between physics, chemistry, colloid science, plasmonics, nanotechnology, and biology. By their very nature, it is impossible to find a textbook that will summarize the principles needed for SERS of these rather dissimilar and disconnected topics. Although a basic understanding of these topics is necessary for research projects in SERS with all its many aspects and applications, they are seldom touched upon as a coherent unit during most undergraduate studies in physics or chemistry. This book intends to fill this existing gap in the literature. It provides an overview of the underlying principles of SERS, from the fundamental understanding of the effect to its potential applications. It is aimed primarily at newcomers to the field, graduate students, researchers or scientists, attracted by the many applications of SERS and plasmonics or its basic science. The emphasis is on concepts and background material for SERS, such as Raman spectroscopy, the physics of plasmons, or colloid science, all of them introduced within the context of SERS, and from where the more specialized literature can be followed. Represents one of very few books fully dedicated to the topic of surface-enhanced Raman spectroscopy (SERS) Gives a comprehensive summary of the underlying physical concepts around SERS Provides a detailed analysis of plasmons and plasmonics
Download or read book Surface Enhanced Raman Scattering written by Katrin Kneipp and published by Springer Science & Business Media. This book was released on 2006-06-21 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Almost 30 years after the first reports on surface-enhanced Raman signals, the phenomenon of surface-enhanced Raman scattering (SERS) is now well established. SERS gained particular interest after single-molecule Raman spectroscopy had been demonstrated. This book summarizes and discusses present theoretical approaches that explain the phenomenon of SERS and reports on new and exciting experiments and applications of the fascinating spectroscopic effect.
Download or read book Surface Enhanced Vibrational Spectroscopy written by Ricardo Aroca and published by John Wiley & Sons. This book was released on 2006-05-01 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface Enhanced Vibrational Spectroscopy (SEVS) has reached maturity as an analytical technique, but until now there has been no single work that describes the theory and experiments of SEVS. This book combines the two important techniques of surface-enhanced Raman scattering (SERS) and surface-enhanced infrared (SEIR) into one text that serves as the definitive resource on SEVS. Discusses both the theory and the applications of SEVS and provides an up-to-date study of the state of the art Offers interpretations of SEVS spectra for practicing analysts Discusses interpretation of SEVS spectra, which can often be very different to the non-enhanced spectrum - aids the practicing analyst
Download or read book Stimulated Raman Scattering Microscopy written by Ji-Xin Cheng and published by Elsevier. This book was released on 2021-12-04 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Download or read book Surface enhanced Raman Scattering Based Immunoassay Platforms for Viral Pathogen Detection written by Jing Neng and published by . This book was released on 2012 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface-enhanced Raman scattering (SERS) based biosensors have been widely applied in biomedical diagnostics for its robustness, high sensitivity, improved levels of multiplexing and potential to perform detection in a point-of-care platform. Recent progress in SERS has been demonstrated for a wide range of biosensor applications, ranging from nucleic acid and immunoassay detection to tumor imaging and cancer diagnosis. SERS-based immunoassays have gained considerable attention for target pathogen detection in food safety and public health assurance. In this work, we have employed different versions of this strategy in the low-level detection of proteins (e.g., immunoglobulins and viral antigens). Therefore, we can detect either the exposure to the pathogen or the existence of pathogen. First, a novel SERS immunoassay for detection of exposure to viral antigen has been developed which used antigen-coated gold nanoparticles (GNPs) for antibody capture and Raman label-conjugated protein A/G (pA/G) as a bi-functional reporter. GNPs were first coated with a recombinant His + -tagged fusion of the West Nile virus (WNV) envelope (E) protein and then incubated with rabbit antiserum raised against recombinant E protein and finally mixed with malachite green (MG)-labeled protein A/G (p/AG). Laser interrogation of the GNP/WNV E antibody/MG-pA/G immunocomplex in solution provided an intense characteristic Raman spectrum of MG which indicated the presence of WNV E antiserum and provided a limit of detection (LOD) of ~2 ng/ml total serum protein. Compared to enzyme-linked immunosorbent assay (ELISA) that were performed using the same antigen/antibody reagents, the LOD for the SERS immunoassay was found to be >400-fold more sensitive. In the next study, we proposed a cost-effective, highly sensitive and reproducible magnetic nanoparticle (MNP) capture-based SERS assay for the detection of two viral antigens. GNPs were conjugated with the capture antibodies and the corresponding Raman reporters, followed by hybridizing with the West Nile virus antigen (WNV) and the Rift Valley fever virus (RVFV) antigen as well as the capture MNPs conjugated with specific antibodies. Using an external magnet source, two different hybridization complexes, Infrared-792 (IR-792) labeled GNP/WNV antigen/MNP and Nile Blue (NB) labeled GNP/RVFV antigen/MNP, were removed from solution followed by laser interrogation of the compacted pellet. Laser excitation of the pelleted material provided a mixed spectrum representing the combination of IR-792 and NB signals obtained from each SERS probes, which is diagnostic for recognition of both antigens. The LOD was ~5 fg/ml for assays conducted in phosphate buffered saline buffer (PBS) and ~25 pg/ml for assays containing PBS spiked with fetal bovine serum. Finally, we demonstrated a robust SERS-based immunoassay for the simultaneous detection of three viral antigens spiked in serum through magnetic capture of the silica-encapsulated SERS nanotags. The specific capture of all the antigens in individual assay reactions was performed by incubation with mixtures of silica-coated nanotags (SiNTGs) and magnetic nanoparticles (MNPs), both conjugated with polyclonal antibodies specific for antigen recognition. The SiNTGs provided distinct spectra that correspond to each antigen capture and were impervious to matrix effects. Detection was provided by magnetic pull-down of the three different SiNTG/antigen/MNP immune-recognition complexes and laser excitation of the three spectrally distinct Raman labels. The LOD was ~ 0.01 ng/ml in 20% fetal bovine serum (FBS) for three antigens, a significant improvement over previous studies in terms of the sensitivity, level of multiplexing and medium complexity.
Download or read book Optical Detection and Structural Analysis of DNA Via Direct Surface enhanced Raman Scattering written by Patricia Gisbert Quilis and published by . This book was released on 2018 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: Un nou mètode basat en la espectroscopia Raman intensificada per superfície (SERS) és implementat en aquesta tesi per a la detecció òptica i anàlisi estructural d'àcids nucleics. Aquest mètode es basa en la utilització de partícules de plata carregades positivament, com a substrats plasmònics per a l'anàlisi d'ADN en solució per SERS. Aquesta estratègia s'ha aplicat amb èxit per a la detecció ultrasensible de mutacions puntuals en el gen K-Ras, les quals són habitualment analitzades amb objectius diagnòstics, especialment en el càncer colorectal. En paral·lel amb aquest estudi, l'impacte estructural d'aquestes partícules amb l'ADN s'analitza amb profunditat.
Download or read book Biosensors Based on Sandwich Assays written by Fan Xia and published by Springer. This book was released on 2018-01-09 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.
Download or read book Detection of Long chain DNA Fragments and Amplification Products Using Surface Enhanced Raman Spectroscopy and Resonance Raman Spectroscopy written by Daniel MacDonald and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advancements in Surface enhanced Raman Scattering Based Bioassays Towards Point of Care Pathogen Detection written by Yina Li and published by . This book was released on 2018 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface-enhanced Raman scattering (SERS), as a versatile technology, has been adapted to a variety of biological studies: identification, detection, tracking and mapping of the target in vivo and in vitro. This dissertation describes approaches towards enabling SERS-based immunoassay for on-site multiplex applications. First, an approach to finely tune an ultrathin shell on the SERS gold nanotags has been optimized, and it improves the sensitivity and specificity of the SERS-based immunoassay. This is of great importance in developing detection of multiple antigens in one single assay. Second, four different Raman spectrally compatible gold nanotags have been synthesized. Given that the SERS-based technology has been demonstrated for its utility in triplex assays for three separate analytes recently, the success of fabricating four different gold nanotags paves the way for a simultaneous quadruplex assay. Third, modification of the recognition biomolecules conjugated on the SERS nanoparticles was proven to be a valid approach to increase the detection sensitivity. Even though the increase was only 1.6 to 3.0 fold, it is worth noting that all the SERS-based immunoassays were performed with a handheld Raman spectrometer. Finally, the SERS-based immunoassay is more than proof of concept, as it has shown its capacity in detecting West Nile virus in avian saliva samples collected in the field with high sensitivity.
Download or read book Microfluidics for Single Cell Analysis written by Jin-Ming Lin and published by Springer Nature. This book was released on 2019-08-28 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Download or read book Nanobiosensors written by Alexandru Grumezescu and published by Academic Press. This book was released on 2016-09-28 with total page 928 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanobiosensors: Nanotechnology in the Agri-Food Industry, Volume 8, provides the latest information on the increasing demand for robust, rapid, inexpensive, and safe alternative technologies that monitor, test, and detect harmful or potentially dangerous foods. Due to their high sensitivity and selectivity, nanobiosensors have attracted attention for their use in monitoring not only biological contaminants in food, but also potential chemical and physical hazards. This book offers a broad overview regarding the current progress made in the field of nanosensors, including cutting-edge technological progress and the impact of these devices on the food industry. Special attention is given to the detection of microbial contaminants and harmful metabolotes, such as toxins and hormones, which have a great impact on both humans and animal health and feed. Includes the most up-to-date information on nanoparticles based biosensors and quantum dots for biological detection Provides application methods and techniques for research analysis for bacteriological detection and food testing Presents studies using analytical tools to improve food safety and quality analysis
