Download or read book Surface Diffusion and Hydrophobic Interactions in Protein Adsorption written by Robert Daymond Tilton and published by . This book was released on 1991 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Polymer Layer Structure and Protein Rearrangements Impact written by and published by . This book was released on 2017 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein adsorption onto foreign material is the beginning of a biological process that results in induced thrombosis that leads to a response that may be detrimental to the body. The prevention of blood plasma protein adsorption is a major factor in promoting biocompatibility of biomaterials with blood plasma. The application of coating biomaterial surfaces with poly(ethylene oxide) (PEO) has been very popular in biocompatibility studies. Theoretical studies have created models that have based protein adsorption on surfaces coated with polymers on free energies (van der Waals, hydrophobic interactions, and steric repulsions). The random sequential adsorption (RSA) model is a theoretical model that postulates that randomly distributed polymers and their obstruction of adsorption sites is a major factor that affects the random placement of protein adsorption based on the availability of the remaining unobstructed adsorption sites. This work examines the simulation of how the arrangement of polymer layers prepared by self-assembled monolayers (SAM) and physical adsorption affects the diffusion of the arrangement of proteins on the surface based on the RSA model. The model in this work extends the RSA model by considering the exact arrangement of polymers on the surface as well as dynamic rearrangements of adsorbed proteins that lead to a more closely packed layer. The modified RSA models are compared to previously reported experimental protein adsorption on PEO grafted surfaces using SAMs as well as physically adsorbed copolymers of PEO. Our results show that accounting for underlying polymer arrangement and protein rearrangements post-adsorption significantly improves the predictions of the RSA model. The simulations are compared with experimental observations for the polymers: EG1OH, EG2OH, EG4OH, EG6OH, EG17OCH3, EG23OH, EG46OH, and EG115OH. The effect of polymer grafting density and polymer chain length of PEO on protein adsorption are also investigated. These results provide a more robust model for predicting protein surface adsorption on hydrophilic polymer coated surfaces as a function of the polymer chain length, grafting density, protein size, protein surface sub-diffusion, and polymer layer structure. This paves a path for the design of high performance anti-fouling surface coatings.
Download or read book Aqueous Protein Adsorption at Solid Surfaces written by Janet Rose Wendorf and published by . This book was released on 2004 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluid Interfaces written by Eduardo Guzmán and published by MDPI. This book was released on 2021-03-02 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluid interfaces are promising candidates for confining different types of materials, e.g., polymers, surfactants, colloids, and even small molecules, to be used in designing new functional materials with reduced dimensionality. The development of such materials requires a deepening of the physicochemical bases underlying the formation of layers at fluid interfaces as well as on the characterization of their structures and properties. This is of particular importance because the constraints associated with the assembly of materials at the interface lead to the emergence of equilibrium and features of dynamics in the interfacial systems, which are far removed from those conventionally found in traditional materials. This Special Issue is devoted to studies on the fundamental and applied aspects of fluid interfaces, and attempts to provide a comprehensive perspective on the current status of the research field.
Download or read book Proteins at Liquid Interfaces written by D. Möbius and published by Elsevier. This book was released on 1998-04-24 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: The interfacial behaviour of surfactants and proteins, and their mixtures, is of importance in a wide range of areas such as food technology, detergency, cosmetics, coating processes, biomedicine, pharmacy and biotechnology. Methods such as surface and interfacial tension measurements and interfacial dilation and shear rheology characterise the relationships between these interfacial properties and the complex behaviour of foams and emulsions is established. Recently-developed experimental techniques, such as FRAP which enable the measurement of molecular mobility in adsorption layers, are covered in this volume. The development of theories to describe the thermodynamic surface state or the exchange of matter for proteins and protein/surfactant mixtures is also described.Features of this book:• Reflects the state-of-the-art research and application of protein interfacial layers rather than a snapshot of only some recent developments.• Emphasis is placed on experimental details as well as recent theoretical developments.• New experimental techniques applied to protein interfacial layers are described, such as FRAP or ADSA, or rheological methods to determine the mechanical behaviour of protein-modified interfaces.• A large number of practical applications, ranging from emulsions relevant in food technology for medical problems such as lung surfactants, to the characterisation of foams intrinsic to beer and champagne production.The book will be of interest to research and university institutes dedicated to interfacial studies in chemistry, biology, pharmacy, medicine and food engineering. Industrial departments for research and technology in food industry, pharmacy, medicine and brewery research will also find this volume of value.
Download or read book Proteins at Solid Liquid Interfaces written by Philippe Déjardin and published by Springer Science & Business Media. This book was released on 2006-09-28 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF–SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.
Download or read book Biopolymers at Interfaces Second Edition written by Martin Malmsten and published by CRC Press. This book was released on 2003-01-15 with total page 930 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition features research from nearly 60 of the profession's most distinguished international authorities. Recognizing emerging developments in biopolymer systems research with fully updated and expanded chapters, the second edition discusses the biopolymer-based multilayer structures and their application in biosensors, the progress made in the understanding of protein behaviour at the air-water interface, experimental findings in ellipsometry and reflectometry, and recent developments concerning protein interfacial behaviour in microfabricated total analysis systems and microarrays. With over 3000 references, this is an essential reference for professionals and students in surface, pharmaceutical, colloid, polymer, and medicinal chemistry; chemical, formulation, and application engineering; and pharmacy.
Download or read book Biological Interactions on Materials Surfaces written by David A. Puleo and published by Springer Science & Business Media. This book was released on 2009-06-26 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Success or failure of biomaterials, whether tissue engineered constructs, joint and dental implants, vascular grafts, or heart valves, depends on molecular-level events that determine subsequent responses of cells and tissues. This book presents the latest developments and state-of-the-art knowledge regarding protein, cell, and tissue interactions with both conventional and nanophase materials. Insight into these biomaterial surface interactions will play a critical role in further developments in fields such as tissue engineering, regenerative medicine, and biocompatibility of implanted materials and devices. With chapters written by leaders in their respective fields, this compendium will be the authoritative source of information for scientists, engineers, and medical researchers seeking not only to understand but also to control tissue-biomaterial interactions.
Download or read book Proteins at Interfaces II written by and published by . This book was released on 1995 with total page 561 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomaterials Science written by Buddy D. Ratner and published by Elsevier. This book was released on 2004-08-18 with total page 879 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this bestselling title provides the most up-to-date comprehensive review of all aspects of biomaterials science by providing a balanced, insightful approach to learning biomaterials. This reference integrates a historical perspective of materials engineering principles with biological interactions of biomaterials. Also provided within are regulatory and ethical issues in addition to future directions of the field, and a state-of-the-art update of medical and biotechnological applications. All aspects of biomaterials science are thoroughly addressed, from tissue engineering to cochlear prostheses and drug delivery systems. Over 80 contributors from academia, government and industry detail the principles of cell biology, immunology, and pathology. Focus within pertains to the clinical uses of biomaterials as components in implants, devices, and artificial organs. This reference also touches upon their uses in biotechnology as well as the characterization of the physical, chemical, biochemical and surface properties of these materials. - Provides comprehensive coverage of principles and applications of all classes of biomaterials - Integrates concepts of biomaterials science and biological interactions with clinical science and societal issues including law, regulation, and ethics - Discusses successes and failures of biomaterials applications in clinical medicine and the future directions of the field - Cover the broad spectrum of biomaterial compositions including polymers, metals, ceramics, glasses, carbons, natural materials, and composites - Endorsed by the Society for Biomaterials
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bioseparations of Proteins written by Ajit Sadana and published by Elsevier. This book was released on 1997-11-27 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation.Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations.Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures.Key Features* Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include:* detailed data on biological stability under various bioseparation conditions* extensive case studies from the literature on separation processes, validation, and economics* simplified analysis of protein refolding and inactivation mechanisms* consideration of adsorption theories and the effect of heterogeneity* coverage of both classical and novel bioseparation techniques, including chromatographic procedures
Download or read book Interactions Involving Lipid based Surfaces written by Victoria Maria Latza and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Interactions involving biological interfaces such as lipid-based membranes are of paramount importance for all life processes. The same also applies to artificial interfaces to which biological matter is exposed, for example the surfaces of drug delivery systems or implants. This thesis deals with the two main types of interface interactions, namely (i) interactions between a single interface and the molecular components of the surrounding aqueous medium and (ii) interactions between two interfaces. Each type is investigated with regard to an important scientific problem in the fields of biotechnology and biology: 1.) The adsorption of proteins to surfaces functionalized with hydrophilic polymer brushes; a process of great biomedical relevance in context with harmful foreign-body-response to implants and drug delivery systems. 2.) The influence of glycolipids on the interaction between lipid membranes; a hitherto largely unexplored phenomenon with potentially great biological relevance. Both problems are addressed with the help of ...
Download or read book Layering Isotherms on Si written by Ofer Isaac Matalon and published by . This book was released on 1999 with total page 692 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Immunoassay Handbook written by David Wild and published by Elsevier. This book was released on 2005-06-20 with total page 968 pages. Available in PDF, EPUB and Kindle. Book excerpt: Containing updated and new information on advanced technology - including micro and nanoscale immunoassays - this text provides a mix of practical information coupled with a review of clincal applications and practical examples.
Download or read book Interactions of Polymers With Bioactive And Corrosive Media written by T. E. Rudakova and published by VSP. This book was released on 1994 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: The successful applications of synthetic polymers in medicine depend mainly on their physico-chemical and special characteristics. This book deals mainly with the kinetic and structural aspects which are essential for the realization of these characteristics.
Download or read book Investigation of Protein Adsorption in Hydrophobic Interaction Chromatographic HIC Systems written by Jie Chen and published by . This book was released on 2007 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
