Download or read book Studying Intercellular Signaling Underlying Human Diseases Using Open Microfluidic Coculture written by Tianzi Zhang and published by . This book was released on 2020 with total page 153 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation focuses on the development of innovative open microfluidic cell culture platforms and their application in studying intercellular signaling underlying human diseases in controlled ex vivo microenvironments. Chapter 1 introduces the background of open microfluidic capillary systems, including the design considerations and current fabrication techniques, and addresses the advantages of using open microfluidic cell culture systems to study intercellular signaling. Chapter 2 presents a new open microfluidic capillary platform, which patterns biocompatible hydrogel walls along a rail insert set inside established cultureware. The permeable hydrogel walls provide segregation for the cells and support diffusion of soluble factors. Chapter 3 discusses a microscale collagen gel contraction assay with an engineered well plate insert that uses surface tension forces to load and manipulate small volumes of cell-laden collagen. The system is easily operated with two pipetting steps and the microscale device moves dynamically as a result of cellular forces. Chapter 4 presents an open microfluidic coculture platform consisting of two independent cell culture regions separated by a half wall. The cell types are selectively seeded into the regions and connected with cell culture media. Using the device, we show that human kidney tubular epithelial cells can tune organ-specificity in other endothelial cell types. Chapter 5 shows a preliminary asthma disease coculture model of human lung fibroblasts and primary alveolar macrophages using a similar open microfluidic coculture device to that described in Chapter 4. The preliminary cell culture result indicates that lung fibroblasts exhibit elevated cell contractility when cultured separately with alveolar macrophages in shared cell culture media. Chapter 6 concludes the thesis and proposes some future research directions and interesting metabolomic studies using existing or alternative open microfluidic systems.
Download or read book Multi Omics for the Understanding of Brain Diseases written by and published by . This book was released on 2021-12-13 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analytical Instrumentation Handbook written by Jack Cazes and published by CRC Press. This book was released on 2004-11-30 with total page 1066 pages. Available in PDF, EPUB and Kindle. Book excerpt: Compiled by the editor of Dekker's distinguished Chromatographic Science series, this reader-friendly reference is as a unique and stand-alone guide for anyone requiring clear instruction on the most frequently utilized analytical instrumentation techniques. More than just a catalog of commercially available instruments, the chapters are wri
Download or read book Angiogenesis Assays written by Carolyn A. Staton and published by John Wiley & Sons. This book was released on 2007-01-11 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology.
Download or read book Microfluidic Lab on a Chip for Chemical and Biological Analysis and Discovery written by Paul C.H. Li and published by CRC Press. This book was released on 2005-11-01 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced, and processes seamlessly integrated. This microscale advantage translates into greater sensitivity, more accurate results, and better information. Microfluidic
Download or read book Endothelial Cell Culture written by Roy Bicknell and published by Cambridge University Press. This book was released on 1996-09-28 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of the Handbooks in Practical Animal Cell Biology is to provide practical workbooks for those involved in primary cell culture. Each volume addresses a different cell lineage, and contains an introductory section followed by individual chapters on the culture of specific differentiated cell types. The authors of each chapter are leading researchers in their fields and use their first-hand experience to present reliable techniques in a clear and thorough manner. Endothelial Cell Culture contains chapters on endothelial cells derived from 1) lung, 2) bone marrow, 3) brain, 4) mammary glands, 5) skin, 6) adipose tissue, 7) female reproductive system, and 8) synovium.
Download or read book Silicon Carbide Technology for Advanced Human Healthcare Applications written by Stephen E. Saddow and published by Elsevier. This book was released on 2022-07-13 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: After over two decades of focused research and development, silicon carbide (SiC) is now ready for use in the healthcare sector and Silicon Carbide Technology for Advanced Human Healthcare Applications provides an up-to-date assessment of SiC devices for long-term human use. It explores a plethora of applications that SiC is uniquely positioned for in human healthcare, beginning with the three primary areas of technology which are closest to human trials and thus adoption in the healthcare industry: neural implants and spinal cord repair, graphene and biosensors, and finally deep tissue cancer therapy using SiC nanotechnology. Biomedical-inspired engineers, scientists, and healthcare professionals will find this book to be very useful in two ways: (I) as a guide to new ways to design and develop advanced medical devices and (II) as a reference for new developments in the field. The book's intent is to stimulate ideas for further technological enhancements and breakthroughs, which will provide alternative solutions for human healthcare applications. - Discusses the utilization of SiC materials for biomedical applications - Provides a logical pathway to understand why SiC is ideal for several critical applications, in particular for long-term implantable devices, and will serve as a guide to new ways to design and develop advanced medical devices - Serves as a reference for new developments in the field and as a technology resource for medical doctors and practitioners looking to identify and implement advanced engineering solutions to everyday medical challenges that currently lack long-term, cost-effective solutions
Download or read book Mucosal Vaccines written by Hiroshi Kiyono and published by Elsevier. This book was released on 1996-10-23 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive, authoritative treatise covers all aspects of mucosal vaccines including their development, mechanisms of action, molecular/cellular aspects, and practical applications. The contributing authors and editors of this one-of-a-kind book are very well known in their respective fields. Mucosal Vaccines is organized in a unique format in which basic, clinical, and practical aspects of the mucosal immune system for vaccine development are described and discussed. This project is endorsed by the Society for Mucosal Immunology. - Provides the latest views on mucosal vaccines - Applies basic principles to the development of new vaccines - Links basic, clinical, and practical aspects of mucosal vaccines to different infectious diseases - Unique and user-friendly organization
Download or read book Neural Crest Stem Cells written by Maya Sieber-Blum and published by World Scientific. This book was released on 2012 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers readers an understanding of the development of neural crest cells, which is crucial as many birth defects and tumours are of neural crest origin. Delving into stem cells from different locations of the body, this book explores the best possible source of such cells for the use in medical applications.
Download or read book Advanced Healthcare Materials written by Ashutosh Tiwari and published by John Wiley & Sons. This book was released on 2014-05-09 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a comprehensive and interdisciplinary view of cutting-edge research on advanced materials for healthcare technology and applications Advanced healthcare materials are attracting strong interest in fundamental as well as applied medical science and technology. This book summarizes the current state of knowledge in the field of advanced materials for functional therapeutics, point-of-care diagnostics, translational materials, and up-and-coming bioengineering devices. Advanced Healthcare Materials highlights the key features that enable the design of stimuli-responsive smart nanoparticles, novel biomaterials, and nano/micro devices for either diagnosis or therapy, or both, called theranostics. It also presents the latest advancements in healthcare materials and medical technology. The senior researchers from global knowledge centers have written topics including: State-of-the-art of biomaterials for human health Micro- and nanoparticles and their application in biosensors The role of immunoassays Stimuli-responsive smart nanoparticles Diagnosis and treatment of cancer Advanced materials for biomedical application and drug delivery Nanoparticles for diagnosis and/or treatment of Alzheimers disease Hierarchical modelling of elastic behavior of human dental tissue Biodegradable porous hydrogels Hydrogels in tissue engineering, drug delivery, and wound care Modified natural zeolites Supramolecular hydrogels based on cyclodextrin poly(pseudo)rotaxane Polyhydroxyalkanoate-based biomaterials Biomimetic molecularly imprinted polymers
Download or read book Organs on chips written by Yu-suke Torisawa and published by MDPI. This book was released on 2020-05-27 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in microsystems technology and cell culture techniques have led to the development of organ-on-chip microdevices that produce tissue-level functionality, not possible with conventional culture models, by recapitulating natural tissue architecture and microenvironmental cues within microfluidic devices. Since the physiological microenvironments in living systems are mostly microfluidic in nature, the use of microfluidic devices facilitates engineering cellular microenvironments; the microfluidic devices allow for control of local chemical gradients and dynamic mechanical forces, which play important roles in cellular viability and function. The organ-on-chip microdevices have great potential to promote drug discovery and development, to model human physiology and disease, and to replace animal models for efficacy and toxicity testing. Recently, induced pluripotent stem (iPS) cells have been leveraged to develop organs-on-chips, which enable various types of organ models and disease models not possible with primary cells and cell lines. This Special Issue seeks to showcase research papers, short communications, and review articles that focus on: (1) microdevices to mimic or control cellular microenvironment; (2) microdevices to evaluate interactions between different organ models; (3) microdevices to maintain iPS cells or iPSC-derived cells; and (4) sensors and techniques to evaluate drug efficacy or toxicity.
Download or read book Flow Cytometry and Cell Sorting written by Andreas Radbruch and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS
Download or read book Microfluidic Cell Culture Systems written by Christopher Bettinger and published by William Andrew. This book was released on 2012-12-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fields of microfluidics and BioMEMS are significantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology. This new professional reference applies the techniques of microsystems to cell culture applications. The authors provide a thoroughly practical guide to the principles of microfluidic device design and operation and their application to cell culture techniques. The resulting book is crammed with strategies and techniques that can be immediately deployed in the lab. Equally, the insights into cell culture applications will provide those involved in traditional microfluidics and BioMEMS with an understanding of the specific demands and opportunities presented by biological applications. The goal is to guide new and interested researchers and technology developers to the important areas and state-of-the-practice strategies that will enhance the efficiency and value of their technologies, devices and biomedical products.
Download or read book Open Microfluidics written by Jean Berthier and published by John Wiley & Sons. This book was released on 2016-07-20 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Open microfluidics or open-surface is becoming fundamental in scientific domains such as biotechnology, biology and space. First, such systems and devices based on open microfluidics make use of capillary forces to move fluids, without any need for external energy. Second, the "openness" of the flow facilitates the accessibility to the liquid in biotechnology and biology, and reduces the weight in space applications. This book has been conceived to give the reader the fundamental basis of open microfluidics. It covers successively The theory of spontaneous capillary flow, with the general conditions for spontaneous capillary flow, and the dynamic aspects of such flows. The formation of capillary filaments which are associated to small contact angles and sharp grooves. The study of capillary flow in open rectangular, pseudo-rectangular and trapezoidal open microchannels. The dynamics of open capillary flows in grooves with a focus on capillary resistors. The case of very viscous liquids is analyzed. An analysis of suspended capillary flows: such flows move in suspended channels devoid of top cover and bottom plate. Their accessibility is reinforced, and such systems are becoming fundamental in biology. An analysis of “rails” microfluidics, which are flows that move in channels devoid of side walls. This geometry has the advantage to be compatible with capillary networks, which are now of great interest in biotechnology, for molecular detection for example. Paper-based microfluidics where liquids wick flat paper matrix. Applications concern bioassays such as point of care devices (POC). Thread-based microfluidics is a new domain of investigation. It is seeing presently many new developments in the domain of separation and filtration, and opens the way to smart bandages and tissue engineering. The book is intended to cover the theoretical aspects of open microfluidics, experimental approaches, and examples of application.
Download or read book Insights in Nanobiotechnology 2022 2023 Novel Developments Current Challenges and Future Perspectives written by Gianni Ciofani and published by Frontiers Media SA. This book was released on 2023-12-12 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are now entering the third decade of the 21st century, and, especially in the last years, the achievements made by scientists have been exceptional, leading to major advancements in the rapidly growing fields of bioengineering and biotechnology. This annual collection, which highlights article submissions from our Editorial Board members, looks to explore new insights, novel developments, current challenges, latest discoveries, recent advances, and future perspectives in the field of Nanobiotechnology. The Research Topic solicits brief, forward-looking contributions that describe the state of the art, outlining recent developments and major accomplishments that have been achieved and that need to occur to move the field forward. Authors are encouraged to identify the greatest challenges in the sub-disciplines, and how to address those challenges. The goal of this special edition Research Topic is to shed light on the progress made in the past decade and on its future challenges to provide a thorough overview of the nanobiotechnology field. This article collection will inspire, inform and provide direction and guidance to researchers. This collection is part of the 'Insights In' series which has been launched in each section of the journal. Other titles in the series include: Insights in Bioprocess Engineering 2022: Novel Developments, Current Challenges, and Future Perspective Insights in Biomaterials 2022: Novel Developments, Current Challenges, and Future Perspectives Insights In Biosafety & Biosecurity 2022: Novel Developments, Current Challenges, and Future Perspectives Insights in Biofabrication 2022: Novel Developments, Current Challenges, and Future Perspectives Insights in Tissue Engineering and Regenerative Medicine 2022 / 2023: Novel Developments, Current Challenges, and Future Perspectives Insights in Synthetic Biology 2022 / 2023: Novel Developments, Current Challenges, and Future Perspectives
Download or read book Cutaneous Wound Healing written by Vincent Falanga and published by CRC Press. This book was released on 2001-08-23 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene therapy, bioengineered skin, and other methods in advanced biology are revolutionizing the treatment of wounds. Written by experts in research and clinical practice, Cutaneous Wound Healing examines the current knowledge and emerging treatment methods. This volume explains the normal molecular and cellular functions that occur when a wound heals, as well as dysfunctional events, such as a chronic wound or an ulcer. Such dysfunctions signal an imbalance in the body, explained here along with possible treatments. The book's mini-atlas is an indispensable reference tool. Dermatologists, plastic surgeons, and general practitioners can benefit from this text.
Download or read book Microfluidic Devices for Biomedical Applications written by Xiujun James Li and published by Woodhead Publishing. This book was released on 2013-10-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications. The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis. Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries.
