Download or read book Study of Charcoal Rot Disease Using the Model Plant Arabidopsis Thaliana written by Priya Fernandes and published by . This book was released on 2013 with total page 83 pages. Available in PDF, EPUB and Kindle. Book excerpt: Macrophomina phaseolina is a warm climate fungal pathogen of the Botryosphaeriaceae family that causes charcoal rot disease in over 700 plant species including commercially grown plants such as soybean, maize, peanut and cotton. Currently used management approaches for the disease including irrigation, addition of organic amendments and crop rotation are not very effective. Limited knowledge on host-pathogen interactions hinders the development of disease resistance strain by genetic engineering. To understand the disease mechanisms at the molecular level, we chose to use Arabidopsis thaliana as a model to study charcoal rot. In this study we established Arabidopsis as a susceptible host for Macrophomina phaseolina. Furthermore, the cellular process of infection and propagation of the pathogen within the host system during the early and late stages of infection were examined by microscopy. Recent studies have demonstrated the crucial roles of different phytohormones in the induction of defense signaling pathways. Here, the interactions between plant hormone-mediated signaling and plant disease resistance were studied using a genetics approach with a hope of understanding the mechanisms of plant immunity against Macrophomina phaseolina mediated by auxin, SA, JA, ET and ABA. In this study we hypothesized that the necrotrophic fungus M. phaseolina invades the host by affecting the biosynthesis of plant hormones (mainly JA, ET and auxin) and/or activation of the corresponding hormonal pathways that are directly or indirectly involved in mounting a defense response against the pathogen. Several mutants that carry mutations in genes that are involved in hormone biosynthesis or signaling were obtained and tested. Results from our study showed that auxin, ET and SA signaling pathways have possible roles of in imparting pathogen resistance against M. phaseolina.
Download or read book Effects of Mutations in GH3 Genes on the Interaction Between the Necrotrophic Fungus Macrophomina Phaseolina and Its Plant Host Arabidopsis Thaliana written by Jordan Brungardt and published by . This book was released on 2014 with total page 66 pages. Available in PDF, EPUB and Kindle. Book excerpt: The necrotrophic soil-borne fungus Macrophomina phaseolina (Tassi) Goid (M. phaseolina) causes the disease charcoal rot in a wide range of plant species worldwide. Many of these species are agronomically important. Attempts to control the disease have been met with little success. Expression of auxin-regulating GH3 genes have been shown to be up-regulated in Medicago plants upon infection with M. phaseolina. Other studies have shown that GH3 knockout mutants have increased resistance to necrotrophic fungal pathogens such as Botrytis cinerea. Based on our previous research, we hypothesized that mutations in GH3 genes in the plant host Arabidopsis thaliana (A. thaliana) would decrease the aggressiveness of M. phaseolina. The research presented here investigates this interaction in an attempt to elucidate the importance of GH3 genes in the disease response pathway. To assess this, available insertion mutants for seven group II GH3 genes of A. thaliana were included in this study. Since GH3 is a multi-gene family and some genes may have functional redundancy, double mutants for two pairs of closely related genes were generated. Homozygous mutants for four of the seven insertion lines were established. Despite being prevalent in other research, homozygosity was unattainable for three of the mutant lines (gh3.2, gh3.4, and gh3.6). The gh3.6 line was found to have an inverted tandem repeat of the T-DNA insert. Although obvious phenotypic differences were present for all the lines, three of the four homozygous lines produced their respective mRNA based on RT-PCR; therefore, they are most likely not a knockout of their perspective gene. The disease phenotypes of these mutant lines were compared to that of the wild type. With three repeats of inoculation, it was concluded that these lines showed no increased resistance to M. phaseolina. Given the data presented here, it is likely GH3 genes play no major role in the pathogenicity of M. phaseolina on the model plant A. thaliana.
Download or read book From Model System to Crops written by Mercedes Mae Schroeder and published by . This book was released on 2018 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants are subject to attack by microbial pathogens. This research utilized four distinct plant-pathosystems combined with methods of phytopathology, molecular genetics and chemical biology to study mechanisms of plant immunity against microbial phytopathogens. Chemical biology is the use of small molecule compounds to study biological processes. A previous chemical screen identified 114 compounds, called synthetic elicitors, that activate innate immune responses in the model plant Arabidopsis thaliana (Arabidopsis). Here, experiments conducted with three synthetic elicitors were found to provide disease protection in one or more of the following plant-pathogen interactions: Solanum lycopersicum (tomato) and Pseudomonas syringae pathovar tomato (Pst), Vigna unguiculata (cowpea) and Fusarium oxysporum formae speciales tracheiphilum race 3 (Fot3) or Arabidopsis and Hyaloperonospora arabidopsidis (Hpa). The results of these studies demonstrated the potential power of synthetic elicitors as both tools for molecular/chemical biology research and for direct protection of agricultural crop plants. In addition to synthetic elicitor-applied research, innovative agar plate-based infection assays were developed to study defense responses in Arabidopsis against Macrophomina phaseolina (Mp). Dubbed as a "global destroyer of crops", the necrotrophic, soil-borne fungus Mp infects more than 500 plant species including many economically important crops. Plant defenses against this powerful pathogen are poorly understood. The novel model phyto-pathosystem was used to quantify Mp biomass growth in roots and to analyze shoot disease severity in different Arabidopsis genotypes, quantitatively assessing host factors affecting the outcome of Mp infections. By comparative profiling of host transcripts in roots of the wild type Arabidopsis accession Col-0, with and without Mp infection, expression changes were uncovered in hundreds of genes potentially contributing to Mp defense mechanisms or putative effector targeting schemes. The results established the Mp-Arabidopsis interaction as a useful model pathosystem, which allowed for application of the vast genomics-related resources of this versatile model plant to the systematic investigation of previously understudied host defenses against a major broad host-spectrum plant pathogen.
Download or read book Genome Scale Analysis of Arabidopsis Thaliana Quantitative Disease Resistance to the Generalist Fungal Pathogen Sclerotinia Sclerotiorum written by Thomas Badet and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In nature, plant pathogen interactions are frequent but disease is not the most prevalent outcome. Indeed, plants evolved an efficient immune system able to face multiple pathogen attacks. Getting insights into plant microbe interactions at multiple levels will improve our understanding of how plants defend against pathogens and help building sustainable agronomy. Fungal plant pathogens are major threats to food security worldwide. Sclerotinia sclerotiorum is an Ascomycete generalist plant pathogen causing mold diseases on hundreds of plant species. There is no genetic source of complete plant resistance to this generalist pathogen known to date. Instead, natural plant populations show a continuum of resistance levels controlled by multiple genes, a phenotype designated as quantitative disease resistance (QDR). Little is known about the molecular mechanisms controlling the interaction between plants and S. sclerotiorum, and more generally which are the molecular bases underlying QDR in plants. My thesis project consisted in a first part in identifying molecular mechanisms underlying QDR to S. sclerotiorum in natural accessions of the model plant Arabidopsis thaliana. A Genome wide association study (GWAS) allowed me to associate genetic variation with disease resistance to S. sclerotiorum. The analysis pinpointed three genes in A. thaliana genome as putative candidates involved in QDR to S. sclerotiorum. I led the functional characterization of these genes and investigated natural diversity at these loci. The results revealed that a prolyl-oligopeptidase (POQR) and an actin-related protein complex member (ARPC4) are associated with QDR against S. sclerotiorum. The analysis of actin filament networks highlighted their role in response to S. sclerotiorum. Furthermore, I showed that POQR alleles evolved convergently in different plant lineages, suggesting that some QDR molecular mechanisms are conserved across plants. Among fungal parasites, some like S. sclerotiorum are able to infect multiple species while others are restricted to one or few hosts. In the second part of the project, I investigated the properties of S. sclerotiorum genome associated to its ability to infect hundreds of plant species. Theory predicts that generalism comes at a cost and may underlie important fitness trade-offs. At the genome level, some codons (nucleotide triplets) allow more efficient translation than their synonymous. Indeed, the genetic encoding of proteins is redundant with multiple codons specifying the same amino acid. The optimization of codon-usage is a mean to reduce the costs associated with protein production. I analysed codon-usage at the genome level in 45 fungal species to reveal that generalist parasites are highly codon optimized. Moreover, I showed that optimized codons are under purifying selection, suggesting that codon optimization is an adaptation to generalist parasitism in fungi.
Download or read book Transactions of the Kansas Academy of Science written by Kansas Academy of Science and published by . This book was released on 2012 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Necrotrophic Fungal Plant Pathogens written by Antonieta De Cal and published by Frontiers Media SA. This book was released on 2022-03-09 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: Paloma Melgarejo is an author on one patent issued in Spain and one patent issued internationally, and has co-obtained plant variety rights for the following strawberry varieties: Aguedilla, Amiga, Carisma, Fontanilla, Fuentepina, Marina, Medina, and Santaclara. Maria Del Mar Jimenez-Gasco is an author on two patents issued in Spain, relating to the identification of Fusarium oxysporum.
Download or read book Microarray Expression Studies in the Model Plant Arabidopsis Thaliana Infected with the Bacterial Pathogen Ralstonia Solanacearum written by Sanushka Naidoo and published by . This book was released on 2008 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Macrophomina Phaseolina written by Pankaj Kumar and published by Elsevier. This book was released on 2023-06-08 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Macrophomina Phaseolina: Ecobiology, Pathology and Management provides insights into the sustainable prevention and treatment of this wide-spread challenge to plant health. With a focus on agricultural as well as non-food plant challenges, the book highlights diverse approaches and provides central insights. The pathogen?of M. phaseolina affects the fibrovascular system of roots and basal internodes of its host, impeding the transport of water and nutrients to the upper parts of the plant, thus resulting in progressive wilting, premature drying and dying, loss of vigor, and reduced yield. Finally, the book explores the interaction of M. phaseolina with soil, microbes and ecological conditions to control disease-causing strategies. Recognizing the necessity that strategies applied to manage the disease should have no or minimum effect on ecological resources, the book focuses on alternative sustainable management technologies such as mix-cropping, the use of antagonistic bacteria and fungi and microbial biosurfactants, plant growth promoting antagonistic rhizobacteria and how cultural practices may also play an important role. Identifies plant diseases caused by Macrophomina phaseolina Explores M. phaseolina genetic diversity Highlights pesticide tolerance and alternative control options
Download or read book Current Trends in Plant Disease Diagnostics and Management Practices written by Pradeep Kumar and published by Springer. This book was released on 2016-04-28 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant diseases play an important role on our daily lives. Most of plant diseases are visible and are caused by biotic and/or abiotic factors. Symptoms are usually the results of a morphological change, alteration or damage to plant tissue and/or cells due to an interference of the plant’s metabolism. All basic structures of vascular plants are subject to attack by pathogens. The failure in accurate disease diagnosis and management may lead to huge losses in plant production and related commodities, which causes nutritional food scarcity. Typically, the appearance of a biotic symptom will indicate the relatively late stage of an infection and/or colonization of a pathogen. Expert detection, accurate diagnosis, and timely management play a significant role in keeping plants free from pathogens. In this book expert scholars share their research knowledge and key literature which are vital toward the diagnosis of plant diseases across the globe, addressing traditional plant pathology techniques, as well as advanced molecular diagnostic approach.
Download or read book Handbook of Maize Its Biology written by Jeff L. Bennetzen and published by Springer Science & Business Media. This book was released on 2008-12-25 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Maize: Its Biology centers on the past, present and future of maize as a model for plant science research and crop improvement. The book includes brief, focused chapters from the foremost maize experts and features a succinct collection of informative images representing the maize germplasm collection.
Download or read book Biological Management of Diseases of Crops written by P. Narayanasamy and published by Springer Science & Business Media. This book was released on 2013-06-05 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological management of diseases of crops is influenced by the nature of interactions between the pathogens and other organisms and the plants. Due to development of resistance in pathogens to fungicides and bactericides, determination of compatibility of biotic biocontrol agents with chemicals is essential for selecting strains of biocontrol agents (BCAs) showing resistance to chemicals to effectively restrict use of the chemicals. Microbial plant pathogens and the antagonists present in the soil and on the plant surfaces are influenced by various cultural practices. It is possible to reduce disease incidence and intensity by crop sanitation and using appropriate rotational crops. Application of physical techniques involving the use of heat, solarization and irradiation has potential to reduce the pathogen population or weaken the potential of pathogens present in the seed, planting materials and soil.
Download or read book Soybean Seed Composition written by Moulay Abdelmajid Kassem and published by Springer Nature. This book was released on 2022-01-10 with total page 638 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soybean Seed Composition covers three decades of advances in quantitative trait loci (QTL) mapping of seed protein, oil, fatty acids, amino acids, sugars, mineral nutrients, isoflavones, lunasin, and other beneficial compounds. It opens with coverage of seed protein, oil, fatty acids, and amino acids and the effects that genetic and environmental factors have on them. Detailed discussion of QTL that control seed protein, oil, and fatty acids follows, and the book also covers seed amino acids, macronutrients, micronutrients, sugars, and other compounds that are key to selection for crop improvement. The book also provides an overview of two decades of QTL mapping of mineral deficiencies in soybean, which sheds light on the importance of a balanced mineral nutrition in soybean and other crops, elucidates salt stress tolerance QTL mapping, which is another challenge that faces soybean and other crop production worldwide. The importance of soybean seed isoflavones from their biosynthesis and quantification methods to locations and variations in seeds, roots, and leaves, to their QTL mapping is discussed, as well as providing key information on lunasin, a bioactive anticancer peptide in soybean seeds that will help farmers and breeders to develop soybean cultivars with improved seed isoflavones and lunasin content. The book will be of interest to graduate students, academics, and researchers in the fields of genetic and QTL mapping of important agronomic traits in soybean and other crops.
Download or read book Bioformulations for Sustainable Agriculture written by Naveen Kumar Arora and published by Springer. This book was released on 2016-06-06 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: More than a century has passed since the first bioformulations were introduced to the market. But there is still much to be done, explored and developed. Though bioformulations offer green alternatives and are important for sustainable agriculture, they make up only a small fraction of the total additions used to enhance crop yields or protect them from pests. There is a great need to develop bioformulations that can promote confidence among end users; accordingly, it is imperative that bioformulations to replace chemicals be reliable and overcome the shortcomings of the past. Bioformulations: for Sustainable Agriculture discusses all the issues related to the current limitations and future development of bioformulations. It examines in detail those bioformulations that include biofertilizers and biopesticides (also commonly known as bioinoculants), presenting a global picture of their development. Further chapters address diverse microbes that are already being or could be used as bioformulations. The book also discusses the techniques, tools and other additions required to establish bioformulations as trustworthy and global solutions. It assesses the types of bioformulations currently available on the market, while also considering the future roles of bioformulations, including the reclamation of marginal and polluted soils. Further, it discusses the current legislation and much-needed amendments. Overall the book provides a comprehensive outlook on the status quo of bioformulations and the future approaches needed to improve them and achieve sustainable agriculture and food security without sacrificing the quality of soils. This will be extremely important in offering chemical-free foods and a better future for generations to come.
Download or read book The Chemical Dialogue Between Plants and Beneficial Microorganisms written by Vivek Sharma and published by Elsevier. This book was released on 2023-07-27 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Chemical Dialogue Between Plants and Beneficial Microorganisms provides foundational insights on plant beneficial microorganisms and their impact on the health and productivity of plants. Providing in-depth and recent updates about unexplored aspects of plant microbes interactions, the book includes the biological repertoire of arbuscular mycorrhizal association, molecular architecture of Rhizobium-plant symbiosis, and endophytes in transcriptional plasticity during host colonization by endophytes. The book also includes details about the mechanism of different plant beneficial microorganisms, how these differ, and their cross signaling. This book will be an important reference for researchers working on different plant beneficial microorganisms and their molecular arsenal. Includes coverage of oxylipins?and sterols in inducing systemic responses Explores the role of microbes in transcriptional plasticity of host plants Highlights the biology of vegetative cells, N2-fixing vesicles, and microbial volatiles in plant growth
Download or read book Abiotic and Biotic Stresses in Soybean Production written by Mohammad Miransari and published by Academic Press. This book was released on 2015-12-31 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abiotic and Biotic Stresses in Soybean Production: Soybean Production Volume One presents the important results of research in both field and greenhouse conditions that guide readers to effectively manage the chemical, physical, and biological factors that can put soybean production at risk. Including the latest in genetics, signaling, and biotechnology, the book identifies these types of stresses, their causes, and means of avoiding, then addresses existing stresses to provide a comprehensive overview of key production yield factors. By presenting important insights into the historical and emerging uses for soybean, the book educates readers on the factors for consideration as new uses are developed. It is an ideal complement to volume two, Environmental Stress Conditions in Soybean Production, that work together to provide valuable insights into crop protection. Presents insights for the successful production of soybean based on chemical, physical and biologic challenges Includes the latest specifics on soybean properties, growth, and production, including responses to different stresses and their alleviation methods Offers recent advancements related to the process of N fixation and rhizobium, including signaling pathways and their practical use Explores the production of rhizobium inoculums at large-scale levels
Download or read book Sclerotinia Diseases of Crop Plants Biology Ecology and Disease Management written by G. S. Saharan and published by Springer Science & Business Media. This book was released on 2008-09-01 with total page 531 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fungus Sclerotinia has always been a fancy and interesting subject of research both for the mycologists and pathologists. More than 250 species of the fungus have been reported in different host plants all over the world that cause heavy economic losses. It was a challenge to discover weak links in the disease cycle to manage Sclerotinia diseases of large number of crops. For researchers and s- dents, it has been a matter of concern, how to access voluminous literature on Sclerotinia scattered in different journals, reviews, proceedings of symposia, workshops, books, abstracts etc. to get a comprehensive picture. With the pub- cation of book on ‘Sclerotinia’, it has now become quite clear that now only three species of Sclerotinia viz. , S. sclerotiorum, S. minor and S. trifoliorum are valid. The authors have made an excellent attempt to compile all the available infor- tion on various aspects of the fungus Sclerotinia. The information generated so far has been presented in different chapters. After introducing the subject various aspects viz. , the diseases, symptomatology, disease assessment, its distribution, economic importance, the pathogen, its taxonomy, nomenclature, reproduction, reproductive structures with fine details, variability, perpetuation, infection and pathogenesis, biochemical, molecular and physiological aspects of host-pathogen interaction, seed infection, disease cycle, epidemiology and forecasting, host resistance with sources of resistance, mechanism of resistance and other mana- ment strategies have been covered.
Download or read book Allele Mining for Genomic Designing of Oilseed Crops written by Chittaranjan Kole and published by CRC Press. This book was released on 2024-09-18 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deliberates on the concept, strategies, tools, and techniques of allele mining in oilseed crops and its application potential in genome elucidation and improvement, including studying allele evolution, discovery of superior alleles, discerning new haplotypes, assessment of intra- and interspecific similarity, and studies of gene expression and gene prediction. Available gene pools in global germplasm collections, specifically consisting of wild allied species and local landraces for almost all major crops, have facilitated allele mining. The development of advanced genomic techniques, including PCR-based allele priming and Eco-TILLING-based allele mining, is now widely used for mining superior alleles. Allele's discovery has become more relevant now for employing molecular breeding to develop designed crop varieties matching consumer needs and with genome plasticity to adapt to climate change scenarios. All these concepts and strategies, along with precise success stories, are presented in the chapters dedicated to the major oilseed crops. 1. This is the first book on the novel strategy of allele mining in oilseed crops for precise breeding. 2. This book presents genomic strategies for mining superior alleles underlying agronomic traits from genomic resources. 3. This book depicts case studies of PCR-based allele priming and Eco-TILLING based allele mining. 4. This book elaborates on gene discovery and gene prediction in major oilseed crops. This book will be useful to students and faculties in various plant science disciplines, including genetics, genomics, molecular breeding, agronomy, and bioinformatics; scientists in seed industries; and policymakers and funding agencies interested in crop improvement.
