Download or read book Studies Toward Improved RNA Detection and Isolation written by Emily Morgan Harcourt and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: As our understanding of molecular biology continues to develop, both general improvements and specialized tools are needed for the progression of RNA detection and analysis. This thesis describes three distinct projects within the broad goal of expanding the techniques available for better interrogation of RNA. Chapter II illustrates the development of a new technique for microRNA detection. MicroRNAs are a class of small regulatory RNAs present in eukaryotes. MicroRNAs pose a challenge for detection because of their small size and sequence homology, but have drawn increasing interest due the predicted prevalence of their gene targets and links between their expression levels and cancer. The development of an isothermal detection strategy is described which incorporates two nucleic acid templated steps, two amplification steps, and fluorescent detection. Up to 3000-fold amplification can be obtained, and 200 pM quantities of let-7a microRNA and above can be detected reliably. In Chapter III, two distinct approaches are taken for the detection of N(6)-methyladenosine, the most common modification in RNA: harnessing base-pairing selectivity, and developing selective reactions. A successful method was developed that harnessed the inherently higher efficiency of a commercially-available polymerase, Tth DNA polymerase, when pairing T with adenosine as opposed to N(6)-methyladenosine. Kinetic analysis showed that the selectivity of the enzyme ranged from 4-18-fold, depending on sequence context. Detection of m6A in ribosomal RNA and in a highly-expressed messenger RNA are demonstrated. The final chapter focuses on a mechanistic approach for enhancing RNA recovery after formaldehyde treatment using an organocatalyst. Mass spectrometry and gel electrophoresis showed an enhanced rate of adduct reversal in the presence of the catalyst, and effects of temperature, buffer, and pH were also analyzed. The results from monomeric and oligomeric test systems have subsequently been applied to recovery of RNA from fixed samples, showing ~2-fold enhancement in recovered RNA due to the catalyst and up to 25-fold enhancement when the catalyst was used in conjunction with optimized time and temperature.
Download or read book Management of Legionella in Water Systems written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2020-02-20 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: Legionnaires' disease, a pneumonia caused by the Legionella bacterium, is the leading cause of reported waterborne disease outbreaks in the United States. Legionella occur naturally in water from many different environmental sources, but grow rapidly in the warm, stagnant conditions that can be found in engineered water systems such as cooling towers, building plumbing, and hot tubs. Humans are primarily exposed to Legionella through inhalation of contaminated aerosols into the respiratory system. Legionnaires' disease can be fatal, with between 3 and 33 percent of Legionella infections leading to death, and studies show the incidence of Legionnaires' disease in the United States increased five-fold from 2000 to 2017. Management of Legionella in Water Systems reviews the state of science on Legionella contamination of water systems, specifically the ecology and diagnosis. This report explores the process of transmission via water systems, quantification, prevention and control, and policy and training issues that affect the incidence of Legionnaires' disease. It also analyzes existing knowledge gaps and recommends research priorities moving forward.
Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2010-07-22 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Download or read book Global Infectious Disease Surveillance and Detection written by Institute of Medicine and published by National Academies Press. This book was released on 2007-11-11 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Early detection is essential to the control of emerging, reemerging, and novel infectious diseases, whether naturally occurring or intentionally introduced. Containing the spread of such diseases in a profoundly interconnected world requires active vigilance for signs of an outbreak, rapid recognition of its presence, and diagnosis of its microbial cause, in addition to strategies and resources for an appropriate and efficient response. Although these actions are often viewed in terms of human public health, they also challenge the plant and animal health communities. Surveillance, defined as "the continual scrutiny of all aspects of occurrence and spread of a disease that are pertinent to effective control", involves the "systematic collection, analysis, interpretation, and dissemination of health data." Disease detection and diagnosis is the act of discovering a novel, emerging, or reemerging disease or disease event and identifying its cause. Diagnosis is "the cornerstone of effective disease control and prevention efforts, including surveillance." Disease surveillance and detection relies heavily on the astute individual: the clinician, veterinarian, plant pathologist, farmer, livestock manager, or agricultural extension agent who notices something unusual, atypical, or suspicious and brings this discovery in a timely way to the attention of an appropriate representative of human public health, veterinary medicine, or agriculture. Most developed countries have the ability to detect and diagnose human, animal, and plant diseases. Global Infectious Disease Surveillance and Detection: Assessing the Challenges-Finding Solutions, Workshop Summary is part of a 10 book series and summarizes the recommendations and presentations of the workshop.
Download or read book RNA Tagging written by Manfred Heinlein and published by Humana. This book was released on 2021-08-08 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.
Download or read book Circular RNAs written by Christoph Dieterich and published by Springer Nature. This book was released on with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nucleic Acids written by Marcelo Larramendy and published by BoD – Books on Demand. This book was released on 2016-03-16 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book, "Nucleic Acids - From Basic Aspects to Laboratory Tools", contains a series of chapters that highlight the development and status of the various aspects of the nucleic acids related to DNA chemistry and biology and the molecular application of these small DNA molecules and related synthetic analogues within biological systems. Furthermore, it is hoped that the information in the present book will be of value to those directly engaged in the handling and use of nucleic acids, and that this book will continue to meet the expectations and needs of all who are interested in the different fascinating aspects of molecular biology.
Download or read book DHEW Publication written by and published by . This book was released on 1976 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant parasitic and Entomogenous Nematode Research written by and published by . This book was released on 1991 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Current Research on Clinical Cancer Diagnosis Therapy and Patient Care written by Smithsonian Science Information Exchange. Current Cancer Research Project Analysis Center and published by . This book was released on 1975 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: 2775 references to research projects being conducted in the United States and elsewhere. Entries arranged under 11 topics, e.g., Cancer therapy, Supportive care of cancer patients, and Rehabilitation. Entries include title, researcher, address, contract number, summary, and supporting agency. Indexes by subjects, investigators, contractors, supporting agencies, and contractor numbers.
Download or read book Generation of cDNA Libraries written by Shao-Yao Ying and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its invention and subsequent development nearly 20 years ago, po- merase chain reaction (PCR) has been extensively utilized to identify numerous gene probes in vitro and in vivo. However, attempts to generate complete and full-length complementary cDNA libraries were, for the most part, fruitless and remained elusive until the last decade, when simple and rapid methods were developed. With current decoding and potential application of human genome information to genechips, there are urgent needs for identification of functional significance of these decoded gene sequences. Inherent in bringing these app- cations to fruition is the need to generate a complete and full-length cDNA library for potential functional assays of specific gene sequences. Generation of cDNA Libraries: Methods and Protocols serves as a laboratory manual on the evolution of generation of cDNA libraries, covering both ba- ground information and step-by-step practical laboratory recipes for which p- tocols, reagents, operational tips, instrumentation, and other requirements are detailed. The first chapter of the book is an overview of the basics of generating cDNA libraries, which include the following: (a) the definition of a cDNA library, (b) different kinds of cDNA libraries, (c) differences between methods for cDNA library generation using conventional approaches and novel stra- gies, including reverse generation of RNA repertoires from cDNA libraries, and (d) the quality of cDNA libraries.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Artificial intelligence A step forward in biomarker discovery and integration towards improved cancer diagnosis and treatment written by Mónica Hebe Vazquez-Levin and published by Frontiers Media SA. This book was released on 2023-04-26 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Diagnostic Prognostic and Predictive Biomarkers in Prostate Cancer written by Carsten Stephan and published by MDPI. This book was released on 2018-06-27 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a printed edition of the Special Issue "Diagnostic, Prognostic and Predictive Biomarkers in Prostate Cancer" that was published in IJMS
Download or read book Circulating Tumor Cells written by Richard J. Cote and published by Springer Nature. This book was released on 2023-08-01 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is well recognized that blood could be the optimal site for evaluating cancer, allowing easy and repeated access for determining prognosis, establishing molecular targets, evaluating the efficacy of therapy, detecting the earliest signs of recurrence, and even detecting cancer at its earliest and most curable stages. The analysis of cancer through blood samples is now known as the liquid biopsy and has been a rich source of research and clinical application. There has been an explosion of interest and progress in liquid biopsy technologies since the first edition of this book. The second edition will expand its focus to now include not only circulating tumor cells (CTC), but also other emerging aspects of the liquid biopsy, including circulating tumor DNA and methylated DNA (ctDNA, ct meDNA), ctRNA, ct miRNA, circulating tumor proteins (and other) biomarkers and circulating tumor derived exosomes (ctExosomes). CTC play a central role in tumor dissemination and metastasis, and have been established as an important evaluative and research tool in advanced cancer, and potentially important in early stage disease. CTC defines tumor cells circulating in blood, while Disseminated Tumor Cells (DTC) refers to tumor cells identified in bone marrow. CTC/DTC are extremely rare events, even in late stage cancer, and their detection has presented enormous technical challenges, with the emergence of multiple technologies developed to address these challenges, including enrichment, identification and sophisticated analytical techniques to evaluate CTC and other cells in circulation that may also be important in the biology of metastasis. As foundational as CTC/DTC has been, the field of liquid biopsy has expanded well beyond these analytes. The relevance of circulating nucleic acids derived from tumor cells has quickly progressed from research to the clinic. There are now well established clinical applications for using ctDNA/RNA to determine therapeutic targets, follow disease progression and detect cancer recurrence long before routine clinical methods. One of the most exciting new areas of work is the possibility of using these circulating tumor derived nucleic acids to detect cancer at its earliest and potentially most curable stages. Another new and burgeoning area is the detection and analysis of ctExosomes. These highly abundant particles which are actively secreted from tumor (and indeed all) cells represent a novel way to detect and define multiple analytes of importance, including proteins, DNA and meDNA, RNA, miRNA, and other cell components that are protected and preserved in these compact structures. This second edition of Circulating Tumor Cells: Advances in Liquid Biopsy Technologies is entirely new and brings together leaders and innovators in the field of liquid biopsy, including basic and molecular biologists, chemists, engineers, statisticians, experts in tumor banking, test developers, research administrators and clinicians. A special feature of this book is that it includes chapters from the members of the US National Cancer Institute Liquid Biopsy Consortium. This edition also includes many of the participants of the latest international meeting on the Advances in Circulating Tumor Cells (ACTC) which is held in Greece every two years and gathers the most important liquid biopsy investigators from around the world. Thus, this edition represents the most comprehensive and up-to-date resource for those who want to further explore the exciting field of CTC and other liquid biopsy technologies. The new edition will be useful to a wide audience including scientists studying metastasis, cancer researchers, translational scientists, oncologic surgeons, medical oncologists, members of the biopharmaceutical industry, and graduate and undergraduate students studying cancer biology.
Download or read book Metagenomics for Microbiology written by Jacques Izard and published by Academic Press. This book was released on 2014-11-07 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concisely discussing the application of high throughput analysis to move forward our understanding of microbial principles, Metagenomics for Microbiology provides a solid base for the design and analysis of omics studies for the characterization of microbial consortia. The intended audience includes clinical and environmental microbiologists, molecular biologists, infectious disease experts, statisticians, biostatisticians, and public health scientists. This book focuses on the technological underpinnings of metagenomic approaches and their conceptual and practical applications. With the next-generation genomic sequencing revolution increasingly permitting researchers to decipher the coding information of the microbes living with us, we now have a unique capacity to compare multiple sites within individuals and at higher resolution and greater throughput than hitherto possible. The recent articulation of this paradigm points to unique possibilities for investigation of our dynamic relationship with these cellular communities, and excitingly the probing of their therapeutic potential in disease prevention or treatment of the future. Expertly describes the latest metagenomic methodologies and best-practices, from sample collection to data analysis for taxonomic, whole shotgun metagenomic, and metatranscriptomic studies Includes clear-headed pointers and quick starts to direct research efforts and increase study efficacy, eschewing ponderous prose Presented topics include sample collection and preparation, data generation and quality control, third generation sequencing, advances in computational analyses of shotgun metagenomic sequence data, taxonomic profiling of shotgun data, hypothesis testing, and mathematical and computational analysis of longitudinal data and time series. Past-examples and prospects are provided to contextualize the applications.
Download or read book Current Research on Selected Aspects of Cancer related Biology written by Current Cancer Research Project Analysis Center (U.S.) and published by . This book was released on 1975 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1350 references to research projects being conducted in the United States and elsewhere. Entries arranged under 7 topics, e.g., Control of cell division, Other tumor pathology, and Host response to tumors. Entries include title, researcher, address, contract number, summary, and supporting agency. Indexes by subjects, investigators, contractors, supporting agencies, and contractor numbers.
