Download or read book Studies on the Specific Interaction of Ribosomal Protein S8 from Escherichia Coli with Its Binding Site in 16S RRNA written by Irina V. Alimova and published by . This book was released on 2001 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Interaction Between Ribosomal Protein S8 and Its Binding Sites on 16S RRNA and Spc MRNA the Minimum Structural Elements of 23S RRNA Required for Binding with Ribosomal Protein L1 written by Lihong Jiang and published by . This book was released on 1994 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Effects of Mutations in the S8 Binding Site of 16S RRNA from Escherichia Coli on the Synthesis of Spc Operon Ribosomal Proteins written by Steven R. King and published by . This book was released on 1989 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ribosomal RNA protein Interactions in Escherichia Coli microform Binding of Ribosomal Protein S20 to 16 S RRNA and Processing of 5 S RRNA by Ribonuclease E written by Robert Stephen Cormack and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 1994 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: The role of the polypeptide encoded by ams/rne/hmp1, a gene required for RNase E activity, was investigated. Northwestern blotting and UV crosslinking of substrate RNA to crude fractions containing RNase E unambiguously showed that overexpressed Ams/Rne/Hmp1 polypeptide has a high affinity for RNA. Our results demonstrate that the ams/rne/hmp1 gene product intimately associates with RNase E substrate RNA and supports the notion that Ams/Rne/Hmp1 is the nuclease responsible for RNase E activity. In addition, the RNA-binding domain of Ams/Rne/Hmp1 was localized and a putative RNase E-associated protein was identified.
Download or read book Ribosomal RNA protein Interactions in Escherichia Coli written by Robert Stephen Cormack and published by . This book was released on 1993 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1997 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Role of Long Range Interactions in Assembly of the Small Subunit of Escherichia Coli Ribosome written by Deepika S. Calidas and published by . This book was released on 2012 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The function of the small subunit (SSU) of the ribosome of Escherichia coli is dependent on dynamic interactions at the intersection of its four domains; namely, the body, platform, head and penultimate stem. The in vitro assembly of each individual domain from its corresponding structural element in 16S ribosomal RNA (rRNA), i.e., the 5', central, 3' major and minor domains and associated ribosomal proteins (r-proteins) has been extensively researched. Less is understood of the long range interactions that occur during assembly as different domains co-assemble, both in vitro and in vivo. Our first approach was to use directed probing from the S8 r-protein as a monitor of SSU assembly. We found that assembly of the neck, a functionally significant region between the head and platform is dependent on assembly of the body. Furthermore, S8 binds two distinct binding sites in 16S rRNA separated by several hundred nucleotides, and the appropriate architecture of the later transcribed region is dependent upon incorporation of r-proteins to the earlier transcribed region. Elements of the body domain, including the 5' terminus do not assume their appropriate conformation except upon assembly of the entire domain. Also, we found that S12 could influence the architecture of the 5' terminus, leading us to examine the role of S12 in 30S subunit assembly, both in vitro and in vivo. S12 possesses a non-canonically structured extension that extends from the solvent surface to the intersubunit surface of the SSU, contacting multiple domains. An almost complete truncation of the extension was unable to support growth, while partial truncations of more than 6 amino acids exhibited growth defects. Truncation of half or all of the extension also resulted in reduced activity of SSUs assembled in vitro. The architecture of ribonucleoprotein complexes assembled with truncated proteins is also altered. The work presented in this thesis elucidates influence of widely separated elements of the SSU on each other during assembly"--Page v.
Download or read book Interaction of 16S and 23S Ribosomal RNA with Functional Ligands Implications for the Mechanism of Protein Synthesis written by Danesh Moazed and published by . This book was released on 1989 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies of Functional Domains in Ribosomal Proteins of the Escherichia Coli 30S Ribosomal Subunit written by Richard C. Conrad and published by . This book was released on 1987 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Modification of the Messenger RNA Binding Site on the 30S Ribosomal Subunits of Escherichia Coli written by Chu Chang and published by . This book was released on 1977 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Nucleic Acids Research written by Gregory W. Warr and published by CRC Press. This book was released on 1990-11-26 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Nucleic Acids Research provides extensively referenced overviews of chapter topics, in addition to step-by-step laboratory protocols. Topics include discussions regarding the preparation and assay of antibodies against oligopeptides, RNA footprinting, gel-retardation assays for nucleic acid binding proteins, in vitro transcription and translation assays for studies of eukaryotic gene expression, human genome mapping, forensic analysis of DNA polymorphism, in situ hybridization for the detection of specific RNA, and other methods. Biochemists, molecular biologists, immunologists, cell biologists, and geneticists will find this book invaluable for their research.
Download or read book Structural and Functional Aspects of Ribosomal Protein S8 RNA Interactions in Escherichia Coli written by Herren Wu and published by . This book was released on 1993 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein RNA Interactions in the 30S Subunit of the E Coli Ribosome and the Three dimensional Structure of 16S RRNA written by Seth Richard Stern and published by . This book was released on 1988 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ribosomes written by Alexander Spirin and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is based on an advanced course of lectures on ribosome structure and protein biosynthesis that I offer at the Moscow State University. These lectures have been part of a general course on molecular biology for almost three decades, and they have undergone considerable evolution as knowledge has been pro gressing in this field. The progress continues, and readers should be prepared that some facts, statements, and ideas included in the book may be incomplete or out of-date. In any case, this is primarily a textbook, but not a comprehensive review. It provides a background of knowledge and current ideas in the field and gives ex amples of observations and their interpretations. I understand that some interpre tations and generalizations may be tentative or disputable, but I hope that this will stimulate thinking and discussing better than if I left white spots. The book has a prototype: it is my monograph "Ribosome Structure and Pro tein Biosynthesis" published by the Benjamin/Cummings Publishing Company, Menlo Park, California, in 1986. Here I have basically kept the former order of pre sentation ofthe topics and the subdivision into chapters. The contents ofthe chap ters, however, have been significantly revised and supplemented. The newly writ ten chapters on translational control in prokaryotes (Chapter 16) and eukaryotes (Chapter 17) are added.
Download or read book Advances in Genetics written by and published by Academic Press. This book was released on 1995-11-14 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Genetics increases its focus on modern human genetics and its relation to medicine with Volume 33 of this long-standing serial. The recent merger of Molecular Genetic Medicine with Advances in Genetics affirms the Academic Press commitment to publish important reviews of the broadest interest to geneticists and their colleagues in affiliated disciplines.In this volume, Petes and Pukkila synthesize the latest research on meiotic recombination, with specific reference to crossover and gene conversions. The "absurd size and complex" structure of the Dystrophin gene is considered in another chapter, with discussions of strategies for future diagnosis and treatment of muscular dystrophy. Two chapters also examine the molecular genetics of sex determination, including the influence of maternal age and resulting chromosomal aberrations. Volume 33 also includes a review of the PAX and HOX gene families and their links to the developmental process, cellular growth control, and forms of cancer. Case studies of thrombophilia, Menkes, and Wilson diseases are used to exemplify the genetic disorders of blood clotting, copper deficiency, and toxicity, respectively. Triman takes a genetic approach to understanding the function of ribosomal RNA using E. coli as the model best able to reveal the inherent complications of the translation process. Leach and O'Connell describe the use of radiation hybrids for constructing high-resolution maps of the human genome. With these reviews the alliance of Molecular Genetic Medicine with Advances in Genetics is completed under the banner of Advances in Genetics.Key Features* Presents technical and historical overviews of molecular biology applied to disease detection, diagnosis, and treatment* Chronicles the continuing explosion of knowledge in molecular genetic medicine by highlighting current approaches to understanding human illness* Documents the revolution in human and molecular genetics leading to a new field of medicine* This volume highlights Analysis of human chromosomes with chapters on pathology of sex determination and numerical chromosomal abnormalities Molecular and genetic bases of muscular dystrophy and Menkes and Wilson diseases Techniques including FISH, IRS-PCR, and radiation hybrids
Download or read book Use of a Cross linking Approach to Study the Interaction of Escherichia Coli 50S subunit Protein L1 with Its Binding Site in the 23S Ribosomal RNA written by Charu Taneja and published by . This book was released on 2002 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemistry of Protein and Nucleic Acid Cross Linking and Conjugation Second Edition written by Shan S. Wong and published by CRC Press. This book was released on 2011-10-10 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the first edition of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, notably multifunctional cross-linkers, have been developed and synthesized. The completion of the human genome project has opened a new area for studying nucleic acid and protein interactions using nucleic acid cross-linking reagents, and advances have also been made in the area of biosensors and microarray biochips for the detection and analysis of genes, proteins, and carbohydrates. In addition, developments in physical techniques with unprecedented sensitivity and resolution have facilitated the analysis of cross-linked products. Updated to reflect the advances of the 21st century, this book offers: An overview of the chemical principles underlying the processes of cross-linking and conjugation A thorough list of cross-linking reagents published in the literature since the first edition, covering monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers Reviews of the use of these reagents in studying protein tertiary structures, geometric arrangements of subunits within complex proteins and nucleic acids, near-neighbor analysis, protein-to-protein or ligand–receptor interactions, and conformational changes of biomolecules Discusses the application of immunoconjugation for immunoassays, immunotoxins for targeted therapy, microarray technology for analysis of various biomolecules, and solid state chemistry for immobilizations
