Download or read book Studies on the Conformation of Adsorbed Proteins written by Peter Billsten and published by . This book was released on 1997 with total page 114 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Static and Hydrodynamic Studies of the Conformation of Adsorbed Macromolecules at the Solid liquid Interface written by David Paul Yavorsky and published by . This book was released on 1983 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Surface Analysis of Adsorbed Proteins written by Elaine Hillenmeyer Tronic and published by . This book was released on 2012 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Adsorbed proteins on surfaces are important in many applications, including medical implants, sensors, marine materials, and in vitro substrates for cell culture and other uses. Understanding the protein structure on the surface would allow better control of the interaction of the material with the surrounding environment and a more reproducible system response. Protein adsorption is a complex process, and characterization requires the combination of multiple analysis techniques. In this thesis, adsorbed protein amounts were measured using x-ray photoelectron spectroscopy (XPS) and radiolabeled proteins. Protein conformation and orientation were measured using time-of-flight secondary ion mass spectrometry (ToF-SIMS), near edge x-ray absorption fine structure (NEXAFS), sum frequency generation (SFG), and enzyme-linked immunosorbent assays (ELISA). Surface type influenced the adsorbed surface concentration of albumin and fibronectin on surfaces of glass, polystyrene, titanium, and sulfonated polystyrene as measured by XPS and radiolabeled protein adsorption. More albumin adsorbed onto polystyrene than glass. More albumin and fibronectin adsorbed onto sulfonated polystyrene than titanium. ToF-SIMS also showed differences in structure of the proteins adsorbed onto the different surfaces. The A1 domain of von Willebrand Factor adsorbed in similar amounts onto glass, tissue culture polystyrene, and polystyrene surfaces, as measured by XPS. However, ToF-SIMS showed differences in solution exposure of A1 domain amino acids when adsorbed onto the three surfaces and NEXAFS showed the most ordered ß-sheet structure when A1 was adsorbed onto polystyrene. ELISA showed lowest binding of antibodies recognizing a nonlinear epitopes within A1 when A1 was adsorbed onto polystyrene. Functional studies using a parallel plate flow chamber measured platelet binding to A1 adsorbed onto the three surfaces. At high shear (20dyne/cm2), platelets showed most detachment from A1 adsorbed onto glass. At low shear (0.2dyne/cm2), platelets showed most detachment from A1 adsorbed onto polystyrene. Surface analysis was also useful in characterizing collagen substrates created under different experimental conditions, including material source and pH. Collagen obtained from different sources exhibited altered adsorption behavior, both in amount adsorbed as measured by XPS and interaction with the A1 domain of von Willebrand Factor as measured by ToF-SIMS. SFG was used to identify differences in ordering of collagen adsorbed from solutions at different pH values. Collagen adsorbed at pH 8.0 showed higher SFG amide signal than collagen adsorbed at pH 6.5, suggesting greater ordering of the peptide backbone at pH 8.0. The differences in SFG signal were not due to the amount adsorbed protein, as XPS showed more collagen adsorbed onto tissue culture polystyrene at pH 6.5 than pH 8.0. These studies demonstrated that the surface type can have a large impact on adsorbed proteins, both in amount adsorbed and surface structure. These studies also showed that surface analysis is very useful in creating defined in vitro protein substrates. In all cases, it was crucial to use multiple analysis techniques to understand these systems.
Download or read book NBS Special Publication written by and published by . This book was released on 1974 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proteins at Interfaces II written by and published by . This book was released on 1995 with total page 561 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proteins at Solid Liquid Interfaces written by Philippe Déjardin and published by Springer Science & Business Media. This book was released on 2006-09-28 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF–SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.
Download or read book Protein Conformation written by Derek J. Chadwick and published by John Wiley & Sons. This book was released on 2008-04-30 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: How the amino acid sequence of a protein determines its three-dimensional structure is a major problem in biology and chemistry. Leading experts in the fields of NMR spectroscopy, X-ray crystallography, protein engineering and molecular modeling offer provocative insights into current views on the protein folding problem and various aspects for future progress.
Download or read book Using Surface Analysis to Investigate how Adsorbed Protein Structure Changes with Controlled Changes in Surface Chemistry written by Rami N. Foster and published by . This book was released on 2015 with total page 127 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein adsorption on synthetic surfaces is an important and widely studied phenomenon in biomaterials research. Despite its importance, it is still poorly understood. Therefore, the ultimate goal of this thesis is to contribute toward a better understanding of the protein-biomaterial interface, with application toward the next generation of biomaterial implants. Narrowing this broad objective down to something more specific, this thesis represents a body of work focused on the interaction of blood plasma proteins with sodium styrene sulfonate (NaSS) grafted surfaces. The approach to achieving this objective is straightforward. First, proof-of-concept must be established that NaSS can be successfully grafted to a given surface. A thorough characterization of NaSS films grafted from titanium and silicon oxide surfaces using atom transfer radical polymerization (ATRP) is presented herein. Next, the grafting procedure must be optimized and scaled in order to reliably produce sufficient quantities of NaSS-grafted samples to meet the demands of protein adsorption studies. Using a 24 factorial experimental design and "activators are continuously regenerated by electron transfer" (ARGET) ATRP chemistry, an optimized NaSS grafting procedure was developed. The findings here may be generally applied as a starting point for grafting other polymers to a variety of surfaces. The last step is application-to use the NaSS films for their intended purpose. Surface analysis techniques were applied to characterize changes in adsorbed bovine serum albumin (BSA), bovine fibrinogen (Fgn), bovine immunoglobulin G (IgG), and bovine plasma films between bare and NaSS-grafted gold surfaces. All three proteins and plasma adsorb more readily to, and have a higher affinity for gold than NaSS surfaces. However, at higher concentrations NaSS adsorbs similar amounts (for plasma) or more (for BSA and Fgn) total protein than gold. The only protein that NaSS surfaces adsorb less of than gold is IgG, because IgG adopts a highly denatured conformation on NaSS. Each adsorbed IgG molecule takes up more space on NaSS compared to gold surfaces, resulting in less total protein adsorbed at all concentrations. Still, with the exception of BSA and plasma on gold surfaces, neither surface appeared to have saturated at the highest protein solution concentration studied. Using principal component analysis (PCA) of just the amino acid ToF-SIMS mass fragments, it was determined that all three proteins and plasma adsorb differently on NaSS and gold surfaces, and that the structure of the xii adsorbed protein films change with surface concentration. One difference between adsorbed films on both surfaces, determined using peak ratios for buried/surface amino acids for each protein, is that adsorbed proteins denature more on NaSS than gold. Also, using peak ratios of non-uniformly distributed amino acids, small differences in average orientations were found between the two surfaces for BSA and IgG films. Finally, principal component (PC) modeling, was used to track changes in adsorbed plasma films with time. On NaSS surfaces the plasma films appear to be more BSA-like at short adsorption times, and more Fgn-like at longer adsorption times. Similarly, on gold surfaces the plasma films on appear to start out more IgG-like and become more Fgn-like with increasing adsorption time. However, the PC model included only the three proteins studied here, where plasma is a complex mixture of hundreds of proteins. Therefore, while both gold and NaSS appear to adsorb more Fgn with time, further study is required to confirm that this is truly representative of the final state of the adsorbed plasma films.
Download or read book Proteins at Liquid Interfaces written by D. Möbius and published by Elsevier. This book was released on 1998-04-24 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: The interfacial behaviour of surfactants and proteins, and their mixtures, is of importance in a wide range of areas such as food technology, detergency, cosmetics, coating processes, biomedicine, pharmacy and biotechnology. Methods such as surface and interfacial tension measurements and interfacial dilation and shear rheology characterise the relationships between these interfacial properties and the complex behaviour of foams and emulsions is established. Recently-developed experimental techniques, such as FRAP which enable the measurement of molecular mobility in adsorption layers, are covered in this volume. The development of theories to describe the thermodynamic surface state or the exchange of matter for proteins and protein/surfactant mixtures is also described.Features of this book:• Reflects the state-of-the-art research and application of protein interfacial layers rather than a snapshot of only some recent developments.• Emphasis is placed on experimental details as well as recent theoretical developments.• New experimental techniques applied to protein interfacial layers are described, such as FRAP or ADSA, or rheological methods to determine the mechanical behaviour of protein-modified interfaces.• A large number of practical applications, ranging from emulsions relevant in food technology for medical problems such as lung surfactants, to the characterisation of foams intrinsic to beer and champagne production.The book will be of interest to research and university institutes dedicated to interfacial studies in chemistry, biology, pharmacy, medicine and food engineering. Industrial departments for research and technology in food industry, pharmacy, medicine and brewery research will also find this volume of value.
Download or read book Publications of the National Institute of Standards and Technology Catalog written by National Institute of Standards and Technology (U.S.) and published by . This book was released on 1976 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Encyclopedia of Surface and Colloid Science written by P. Somasundaran and published by CRC Press. This book was released on 2006 with total page 962 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Publications written by United States. National Bureau of Standards and published by . This book was released on 1976 with total page 620 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Journal of Research of the National Bureau of Standards written by United States. National Bureau of Standards and published by . This book was released on 1977 with total page 1046 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Publications of the National Bureau of Standards 1975 Catalog written by United States. National Bureau of Standards and published by . This book was released on 1976 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Publications of the National Bureau of Standards Catalog written by United States. National Bureau of Standards and published by . This book was released on 1975 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair written by Mario Barbosa and published by Woodhead Publishing. This book was released on 2017-09-25 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair highlights the various important considerations that go into biomaterial development, both in terms of fundamentals and applications. After covering a general introduction to protein and cell interactions with biomaterials, the book discusses proteins in biomaterials that mimic the extracellular matrix (ECM). The properties, fabrication and application of peptide biomaterials and protein-based biomaterials are discussed in addition to in vivo and in vitro studies. This book is a valuable resource for researchers, scientists and advanced students interested in biomaterials science, chemistry, molecular biology and nanotechnology. - Presents an all-inclusive and authoritative coverage of the important role which protein and peptides play as biomaterials for tissue regeneration - Explores protein and peptides from the fundamentals, to processing and applications - Written by an international group of leading biomaterials researchers
Download or read book Biopolymers at Interfaces Second Edition written by Martin Malmsten and published by CRC Press. This book was released on 2003-01-15 with total page 930 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition features research from nearly 60 of the profession's most distinguished international authorities. Recognizing emerging developments in biopolymer systems research with fully updated and expanded chapters, the second edition discusses the biopolymer-based multilayer structures and their application in biosensors, the progress made in the understanding of protein behaviour at the air-water interface, experimental findings in ellipsometry and reflectometry, and recent developments concerning protein interfacial behaviour in microfabricated total analysis systems and microarrays. With over 3000 references, this is an essential reference for professionals and students in surface, pharmaceutical, colloid, polymer, and medicinal chemistry; chemical, formulation, and application engineering; and pharmacy.
