Download or read book Studies on the Biological Functions of DNA Helicases I and II of the Bacterium Escherichia Coli written by Mo-Quen Klinkert and published by . This book was released on 1979 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structural and Biochemical Studies of Replicative Helicase Loading in Bacteria written by Iris Vanessa Hood and published by . This book was released on 2015 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: The initiation of DNA replication represents a defining commitment to the proliferation of all cellular organisms. Dedicated ATP-dependent initiation factors specifically mark replication start sites or "replication origins" and, together with ATP-dependent helicase loaders, help coordinate productive assembly of the replisome. In many bacterial species, helicase loaders belonging to the DnaC/I family of proteins assist the bacterial initiator factor DnaA with loading and activation of DnaB-type replicative helicases. Despite advances in understanding some of the essential proteins and common principles underlying initiation strategies, major questions still persist in defining how initiation programs are executed at the molecular level. The present dissertation presents a combination of structural and biochemical studies of the DnaC helicase loader and DnaA initiator from the Gram-negative bacterium Escherichia coli (E. coli) and the DnaI helicase loader from the Gram-positive bacterium Staphylococcus aureus (S. aureus). For the E. coli work, I developed several fluorescence-based helicase assays to help define the particular contributions of both DnaA and DnaC in promoting loading of DnaB at a replication origin. Investigations of DnaA mutants in this helicase assay revealed the importance of the initiator's N-terminal helicase binding domain in recruiting DnaB to a nascent bubble or fork to facilitate helicase loading. Parallel studies of the E. coli DnaC demonstrated: (i) that the loader's N-terminal DnaB binding domain is sufficient for activation of E. coli DnaB's duplex DNA unwinding activity and (ii) that the AAA+-family ATP binding and hydrolysis domain of DnaC likely serves as a regulatory element that helps enhance the efficiency of helicase activation. To better characterize the activity of a Gram-positive S. aureus DnaI helicase loader, I structurally and biochemically characterized the protein's ATPase domain and activity, and also investigated how a particular viral peptide inhibitor from phage 77, termed "ORF104", interferes with host DNA replication by blocking SaDnaI activity. Comparative structural analyses, combined with biochemical studies of SaDnaI revealed not only how the viral inhibitor 77ORF104 blocks loader function but also revealed insights into how bacterial helicase loaders may in general auto-regulate their function. A complimentary viral-host interaction study of another staph-specific viral peptide that also inhibits host DNA replication ("ORF078" from phage 71) has revealed another promising anti-bacterial strategy: preliminary binding studies indicate that this gene product binds primase through its helicase-binding domain. Overall, the studies presented in this dissertation provide multiple new insights into fundamental mechanisms underlying the initiation of DNA replication. One is an enhanced understanding of the role that the E. coli DnaA initiator and DnaC loader serve in recruiting the DnaB helicase to a replication origin. The other describes a novel viral mechanism for inhibition of the S. aureus DnaI helicase loader that helps establish how a virus may exploit an existing auto-regulatory element of the bacterial helicase loader as part of a strategy to inhibit host DNA replication. These findings collectively not only answer long-standing questions in the field, but also open up new avenues for future inquiry.
Download or read book Helicases from All Domains of Life written by Renu Tuteja and published by Academic Press. This book was released on 2018-10-15 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: Helicases are ubiquitous enzymes found throughout evolution. Research in the helicase field has been going on for a long time now but in recent past with the completion of so many genomes, these enzymes have been discovered in a number of organisms. But the available literature is scattered. The huge number of identified DNA and RNA helicases, along with the structural and functional differences among them, make difficult for the interested scholar to grasp a comprehensive view of the field. Helicases from all Domains of Life is the first book to compile information about helicases from many different organisms in one place. Knowledge of the functions and features of helicases across the different kingdoms of life are a valuable source of novel ideas and information The book begins with a chapter on the evolutionary history of helicases followed by three "overview" chapters: one for bacteria/archaea (which are not mentioned), one for plants/algae and one for human helicases The overview chapters are followed by specific chapters on selected helicases of great importance from a biological/applicative point of view
Download or read book Research Awards Index written by and published by . This book was released on 1989 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analysis of the Role of DNA Helicase II uvrD in DNA Replication in Escherichia Coli written by Sandeep Bhat and published by . This book was released on 1996 with total page 78 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomedical Index to PHS supported Research written by and published by . This book was released on 1991 with total page 1104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Purification and Characterization of the Escherichia Coli Helicase II Protein and a Study of the Protein s Enzymatic Activities written by Gregory Thomas Runyon and published by . This book was released on 1991 with total page 620 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Escherichia Coli DNA Helicase II written by Mark Christopher Hall and published by . This book was released on 1998 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mechanistic Studies of the DNA Helicase II Protein of E coli written by Jaya G. Yodh and published by . This book was released on 1993 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Recombinational Repair of DNA Damage written by Andrei Kuzminov and published by Landes Bioscience. This book was released on 1996 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomedical Index to PHS supported Research pt A Subject access A H written by and published by . This book was released on 1992 with total page 1192 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA Helicases written by Eckhard Jankowsky and published by Royal Society of Chemistry. This book was released on 2010-07-01 with total page 303 pages. Available in PDF, EPUB and Kindle. Book excerpt: RNA helicases and RNA helicase-like proteins are the largest group of enzymes in eukaryotic RNA metabolism and although they are subject to intense ongoing research there is much confusion about function and classification of these enzymes. Although these enzymes are essential for virtually all processes involving RNA, there is no overview detailing structure, function and/or biological roles of these pivotal proteins. This book provides the first comprehensive and systematic overview of biology, mechanism, and structure of RNA helicases and RNA helicase-like enzymes. Research into RNA helicases takes place in many different fields from cell and developmental biology to mechanistic enzymology, and structural biology and this book integrates the knowledge of these diverse fields into one valuable resource. It also provides an informative overview on the entire group of enzymes. Individual chapters on each subfamily of RNA helicases and RNA helicase-like proteins are written by experts in the respective fields. All chapters are systematically integrated and the reader is guided by a didactic introductory chapter. The main strengths of the book are the combination of systematics and details that will allow the reader to gain insight into results from diverse fields while maintaining a view of the entire field. It will be a key reference for academics, advanced students, researchers and professionals working in or joining this field.
Download or read book Physical Biology of the Cell written by Rob Phillips and published by Garland Science. This book was released on 2012-10-29 with total page 1089 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physical Biology of the Cell is a textbook for a first course in physical biology or biophysics for undergraduate or graduate students. It maps the huge and complex landscape of cell and molecular biology from the distinct perspective of physical biology. As a key organizing principle, the proximity of topics is based on the physical concepts that
Download or read book Encyclopedia of Biological Chemistry written by and published by Academic Press. This book was released on 2013-01-08 with total page 3223 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 4-volume Encyclopedia of Biological Chemistry, Second Edition, represents the current state of a dynamic and crucial field of study. The Encyclopedia pulls together over 500 articles that help define and explore contemporary biochemistry, with content experts carefully chosen by the Editorial Board to assure both breadth and depth in its coverage. Editors-In-Chief William J. Lennarz and M. Daniel Lane have crafted a work that proceeds from the acknowledgement that understanding every living process-from physiology, to immunology, and genetics-is impossible without a grasp on the basic chemistry that provides its underpinning. Each article in the work provides an up-to-date snapshot of a given topic, written by experts, as well as suggestions for further readings for students and researcher wishing to go into greater depth. Available on-line via SciVerse ScienceDirect, the functionality of the Encyclopedia will provide easy linking to referenced articles, electronic searching, as well an online index and glossary to aid comprehension and searchability. This 4-volume set, thoroughly up-to-date and comprehensive, expertly captures this fast-moving field Curated by two esteemed editors-in-chief and an illustrious team of editors and contributors, representing the state of the field Suggestions for further readings offer researchers and students avenues for deeper exploration; a wide-ranging glossary aids comprehension
Download or read book Molecular Biology written by Jordanka Zlatanova and published by Taylor & Francis. This book was released on 2023-03-21 with total page 732 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology: Structure and Dynamics of Genomes and Proteomes second edition illustrates the essential principles behind the transmission and expression of genetic information at the level of DNA, RNA, and proteins. Emphasis is on the experimental basis of discovery and the most recent advances in the field while presenting a rigorous, yet still concise, summary of the structural mechanisms of molecular biology. Topics new to this edition include the CRISPR-Cas gene editing system, Coronaviruses – structure, genome, vaccine and drug development, and newly recognized mechanisms for transcription termination. The text is written for advanced undergraduate or graduate-level courses in molecular biology. Key Features · Highlights the experimental basis of important discoveries in molecular biology. · Thoroughly updated with new information on gene editing tools, viruses, and transcription mechanisms, termination and antisense. · Provides learning objectives for each chapter. · Includes a list of relevant videos from the Internet about the topics covered in the chapter.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1985 with total page 1104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
