Download or read book Studies on Protein Secretion to the Escherichia Coli K 12 Outer Membrane Using PhoA Gene Fusions written by Charles S. Hoffman and published by . This book was released on 1986 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation and biogenesis of outer membrane Pho E protein of Escherichia coli K 12 written by Johannes P. Tommassen and published by . This book was released on 1982 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biogenesis of outer membrane protein PhoE of Escherichia coli K 12 written by Hans de Cock and published by . This book was released on 1991 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biogenesis of Outer Membrane Protein PhoE of Escherichia Coli K 12 written by Johannes Jacobus Petrus Antonius de Cock and published by . This book was released on 1991 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Studies on the Regulation of the Major Outer Membrane Porin Proteins of Escherichia Coli K 12 written by Michael Nip Hall and published by . This book was released on 1981 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Proceedings of the National Academy of Sciences of the United States of America written by National Academy of Sciences (U.S.). and published by . This book was released on 1985 with total page 756 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Membrane Protein Assembly written by Gunnar von Heijne and published by R. G. Landes. This book was released on 1997 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Use of Beta lactamase Fusions to Study Membrane Protein Assembly and Protein Export in Escherichia Coli written by Yianbiao Zhang and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Use of Beta lactamase Fusions to Study Membrane Protein Assembly and Protein Export in Escherichia Coli written by and published by . This book was released on 1991 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A genetic system for directly expressing proteins in E. coli and assessing their ability to insert into the bacterial cytoplasmic membrane was developed, consisting of a direct expression vector, and a mature P-lactamase cassette plasmid for generating translational fusions of P-lactamase to the bacterially expressed proteins. This system was used to analyse the fate of three different eukaryotic plasma membrane proteins in E. coli. The ampicillin resistance/sensitivity phenotypes of the P-lactamase fusion proteins revealed that the P-subunit of the sheep-kidney Na,K-ATPase, a class II membrane protein, inserts into the cytoplasmic membrane of E. coli, with the same topology as in its native membrane. The Alzheimer's disease amyloid A4 protein precursor, a class I membrane protein, was also found to insert into the bacterial cytoplasmic membrane, and, although preliminary, the results were compatible with its correct topological insertion into the bacterial cytoplasmic membrane. In contrast, the P2-adrenergic receptor, a complex membrane protein that has an extracellular amino-terminal domain and seven transmembrane segments, was found to misinsert into the E. coli cytoplasmic membrane. A plasmid vector for cloning sequences able to function as cytoplasmic membrane translocators of P-lactamase in E. coli was also developed. Short DNA fragments of essentially random sequence were cloned, and recombinants with functional translocators were directly selected. The spectrum of random sequences able to function as bacterial cytoplasmic membrane translocators suggests that the hydrophobicity requirements for translocation across the bacterial cytoplasmic membrane and yeast endoplasmic reticulum membrane may be more similar than previously assumed.In the above studies new and widely applicable uses of P-lactamase fusion technology have been described. As applied in this thesis they highlight similarities and differences between the mechanisms by which proteins assemble into, or are translocated across, the bacterial cytoplasmic membrane and the eukaryotic endoplasmic reticulum membrane.

Download or read book Genetic Studies on Protein Folding and Protein Secretion in Escherichia Coli written by Georg Jander and published by . This book was released on 1996 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Assembly of Proteins in the Outer Membrane of Escherichia Coli K 12 written by John Hagen Carlson and published by . This book was released on 1993 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Journal of Cellular Biochemistry written by and published by . This book was released on 1983 with total page 660 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Genetic Study of Membrane Protein Insertion and Disulfide Bond Formation in Escherichia Coli K 12 written by Hongping Tian and published by . This book was released on 2001 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structure function relationship of outer membrane protein PhoE of Escherichia coli K 12 written by Marlies Cornelia Struyvé and published by . This book was released on 1992 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein Secretion Studies Using E coli Alkaline Phosphatase as a Model System written by Ping Li and published by . This book was released on 1988 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation and Biogenesis of Outer Membrane PhoE Protein of Escherichia Coli K 12 written by Johannes Petrus Maria Tommassen and published by . This book was released on 1982 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bacterial Protein Secretion Systems written by Laure Journet and published by Humana Press. This book was released on 2017-07-01 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.