Download or read book Studies of the Ribosomal Binding Sites of and Cooperativity of Binding of Protein Synthesis Initiation Factors of Escherichia Coli written by Steven Robert Langberg and published by . This book was released on 1978 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Composition of Ribosomal Binding Sites for Initiation Factors in Escherichia Coli written by Ronald Lee Heimark and published by . This book was released on 1976 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluorescence Polarization Studies of the Binding of Escherichia Coli Protein Synthesis Initiation Factor One to 30S Ribosomal Subunits written by Frank Howard Zucker and published by . This book was released on 1984 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluorescence Polarization Studies of the Interaction of Escherichia Coli Protein Synthesis Initiation Factors with Ribosomes written by Jurgen Weiel and published by . This book was released on 1980 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Mechanism of Initiation of Protein Synthesis in Escherichia Coli written by Nicolaas Naaktgeboren and published by . This book was released on 1976 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Synthesis Initiation Factor IF 2 from Escherichia Coli written by John Leonard Fakunding and published by . This book was released on 1974 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Determining the Ribosome Binding Footprint in Escherichia Coli to Improve the Ribosome Binding Site Calculator Model of Translation Initiation written by Ashlee Smith and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last 20 years, the technology used to create synthetic DNA has greatly advanced. Today, DNA can now be synthesized 3 times as quickly and costs 5 times less. The availability of recombinant DNA systems provides flexibility for researchers in academia and industry trying to solve metabolic engineering problems and elucidate biological functions. One common metabolic engineering problem is developing a system to control the expression of a protein of interest over a range from very little to very large expression. Instead of generating large combinatorial libraries to find a sequence with a specific function, which is time consuming and inefficient, researchers are rationally altering the DNA sequence to achieve a desired attribute using information gained from biophysical modeling. One such alteration is the design of synthetic ribosome binding sites to improve the rate of translation initiation in order to increase the expression of protein. The Ribosome Binding Site Calculator (RBS) is software that utilizes a biophysical model of translation initiation to allow researchers to predict the translation initiation rate of a particular sequence or generate a synthetic RBS with a specified translation initiation rate. There are many interactions that take place during translation initiation. In order to improve the accuracy of the model's predictions, one such interaction that we studied is the ribosomal footprint. The ribosome must unfold a section of messenger RNA (mRNA) after the start codon and load it into an mRNA channel. This channel can only accommodate single stranded mRNA. The number of nucleotides in the coding section the ribosome must unfold and load into the channel is the ribosomal footprint (FP). We hypothesized that a secondary structure contained in the ribosomal footprint must be unfolded and would greatly decrease protein expression, and that a secondary structure outside of the footprint region would not have to be unfolded and protein expression would be higher. We identified the ribosomal footprint for Escherichia coli by creating a high expressing RBS sequence and a modified coding section appended to the red fluorescent protein (RFP) reporter gene. The modified coding section featured a movable hairpin structure that was moved from immediately after ATG (position 3) to position 39 to show the changes in protein expression. We found that translation initiation gradually increased from very low levels when the hairpin was close to the start codon to over 1000-fold higher after the hairpin had passed the footprint region. The footprint was found to be 13 or 14 nucleotides including the start codon. Discrepancies between the model predictions and the data revealed the free energy the ribosome must expend to load the mRNA into the channel. The average error for constructs with a hairpin located 3 to 10 nucleotides into the coding sequence was determined to be 7.7 kcal/mol. This value represents the free energy cost of secondary structure within the footprint region, [delta]Gload. This information improves the biophysical model by determining the free energy change of loading the mRNA into the channel and will provide researchers with more accurate predictions of translation initiation.
Download or read book Studies on the Interaction Between Ribosomal Subunits of Escherichia Coli written by Otto Paul van Diggelen and published by . This book was released on 1973 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Mechanism of Initiation of Protein Synthesis in Escherichia Coli written by Robert Benne and published by . This book was released on 1973 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Interaction of Translation Initiation Factor IF1 with the E Coli Ribosomal A Site written by Kam Dawnell Dahlquist and published by . This book was released on 2000 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Inhibition of Protein Synthesis in Escherichia Coli by Expression of RNAs Containing Multiple Ribosome Binding Sites written by Mary V. Mawn and published by . This book was released on 2000 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Characterization of the Binding of Escherichia Coli Initiation Factor 3 to Polynucleotides written by Brian Frederick Schmidt and published by . This book was released on 1983 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Polypeptide Chain Initiation and the Characterization of Ribosomal Binding Sites in E Coli written by and published by . This book was released on 1966 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Effects of Heterologous Ribosomal Binding Sites on the Expression of the Lacz Gene of Escherichia Coli written by Alfred Cornelis Looman and published by . This book was released on 1986 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Physiological Effects of Initiation Factor Or Ribosomal Protein Limitation in Escherichia Coli written by Christine Louise Olsson and published by . This book was released on 1993 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies on the Specific Interaction of Ribosomal Protein S8 from Escherichia Coli with Its Binding Site in 16S RRNA written by Irina V. Alimova and published by . This book was released on 2001 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Critique on the Mechanism of Transfer Ribonucleic Acid Binding to the Bacterial Ribosome written by Stephen Harding Forbes and published by . This book was released on 1974 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:
