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Book Interaction of Escherichia Coli RNA Polymerase Holoenzyme Containing Sigma 32 with Promoters for Heat Shock Genes

Download or read book Interaction of Escherichia Coli RNA Polymerase Holoenzyme Containing Sigma 32 with Promoters for Heat Shock Genes written by Deborah Walker Cowing and published by . This book was released on 1988 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Studying the Interaction of the E Coli Sigma Factors with Core RNA Polymerase Using LRET

Download or read book Studying the Interaction of the E Coli Sigma Factors with Core RNA Polymerase Using LRET written by Bryan T. Glaser and published by . This book was released on 2008 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Interaction of Escherichia Coli Sigma Factors with Core RNA Polymerase

Download or read book Interaction of Escherichia Coli Sigma Factors with Core RNA Polymerase written by Larry C. Anthony and published by . This book was released on 2003 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Molecular Interactions Between the Transcription Factor Crl and Sigma S RNA Polymerase Holoenzyme in Escherichia Coli

Download or read book Molecular Interactions Between the Transcription Factor Crl and Sigma S RNA Polymerase Holoenzyme in Escherichia Coli written by and published by . This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteria must change their cellular composition in response to changing environmental conditions and stress. They implement these changes by altering gene expression and/or protein activity. Central to changes in gene expression during stress responses is regulation of transcription. In bacteria, transcription is directed by a suite of RNA polymerase (RNAP) holoenzymes, comprised of a core enzyme, possessing the catalytic activity for RNA synthesis, and one of a collection of transcription initiation factors, known as Ï3 factors, which recognizes promoter DNA sequences upstream of genes. Increasing the formation of a particular type of RNAP holoenzyme by switching the Ï3 factor will lead to expression of its cognate regulon. My thesis research focuses on deciphering the complex molecular interactions between the transcription factor Crl and the general stress response sigma factor, Sigma S, in order to understand Escherichia coli's response to stress at the molecular level. Crl is a small (133 amino acids in E. coli) protein that stimulates transcription mediated by Sigma S. At the outset of my research, little was known about how Crl interacts with the transcription machinery to stimulate Sigma S-mediated transcription. I used a combination of biochemical, genetic, and molecular biological techniques in vitro and in vivo to identify key interactions and gain insight into the mechanism of transcriptional regulation by Crl. I identify a key binding determinant (the DPE motif) in Sigma S conserved domain 2 underlying its specific recognition by Crl. I demonstrate directly that Crl requires this determinant for positive regulation of Sigma S-mediated transcription and to enhance Sigma S holoenzyme formation. I made the primary Sigma factor recognizable by Crl by substitution of the DPE motif along with deletion of a large non-conserved region (NCR). I also localized the area of Crl required for the interaction with Sigma S to Crl's conserved central cleft. Finally, I identified an interaction between the Beta prime subunit of core RNAP and Crl, which occurs only in the context of the holoenzyme, that has begun to clarify the mechanism by which Crl functions as a Sigma S-specific RNAP holoenzyme assembly factor.

Book Cumulated Index Medicus

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1997 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Physicochemical Studies of E Coli RNA Polymerase Holoenzyme Core Enzyme and Sigma Subunit

Download or read book Physicochemical Studies of E Coli RNA Polymerase Holoenzyme Core Enzyme and Sigma Subunit written by Peter Dean Orphanos and published by . This book was released on 1980 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book The Sigma F RNA Polymerase of Escherichia Coli

Download or read book The Sigma F RNA Polymerase of Escherichia Coli written by David Neil Arnosti and published by . This book was released on 1989 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Studies on the Function and Regulation of Heat Shock Proteins InEscherichia Coli

Download or read book Studies on the Function and Regulation of Heat Shock Proteins InEscherichia Coli written by David Bradley Straus and published by . This book was released on 1989 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book An Analysis of the sigma core Interface in E Coli

Download or read book An Analysis of the sigma core Interface in E Coli written by Meghan M. Sharp and published by . This book was released on 2001 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Molecular Chaperones in the Cell

Download or read book Molecular Chaperones in the Cell written by Peter A. Lund and published by Oxford University Press, USA. This book was released on 2001 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this text, leading experts synthesise our body of knowledge , and the reader gains not only a fuller understanding of the roles of chaperones in the context of cellular processes, but also an insight into the nature of these proteins.

Book Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria

Download or read book Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria written by Frans J. de Bruijn and published by John Wiley & Sons. This book was released on 2016-07-01 with total page 1464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteria in various habitats are subject to continuously changing environmental conditions, such as nutrient deprivation, heat and cold stress, UV radiation, oxidative stress, dessication, acid stress, nitrosative stress, cell envelope stress, heavy metal exposure, osmotic stress, and others. In order to survive, they have to respond to these conditions by adapting their physiology through sometimes drastic changes in gene expression. In addition they may adapt by changing their morphology, forming biofilms, fruiting bodies or spores, filaments, Viable But Not Culturable (VBNC) cells or moving away from stress compounds via chemotaxis. Changes in gene expression constitute the main component of the bacterial response to stress and environmental changes, and involve a myriad of different mechanisms, including (alternative) sigma factors, bi- or tri-component regulatory systems, small non-coding RNA’s, chaperones, CHRIS-Cas systems, DNA repair, toxin-antitoxin systems, the stringent response, efflux pumps, alarmones, and modulation of the cell envelope or membranes, to name a few. Many regulatory elements are conserved in different bacteria; however there are endless variations on the theme and novel elements of gene regulation in bacteria inhabiting particular environments are constantly being discovered. Especially in (pathogenic) bacteria colonizing the human body a plethora of bacterial responses to innate stresses such as pH, reactive nitrogen and oxygen species and antibiotic stress are being described. An attempt is made to not only cover model systems but give a broad overview of the stress-responsive regulatory systems in a variety of bacteria, including medically important bacteria, where elucidation of certain aspects of these systems could lead to treatment strategies of the pathogens. Many of the regulatory systems being uncovered are specific, but there is also considerable “cross-talk” between different circuits. Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria is a comprehensive two-volume work bringing together both review and original research articles on key topics in stress and environmental control of gene expression in bacteria. Volume One contains key overview chapters, as well as content on one/two/three component regulatory systems and stress responses, sigma factors and stress responses, small non-coding RNAs and stress responses, toxin-antitoxin systems and stress responses, stringent response to stress, responses to UV irradiation, SOS and double stranded systems repair systems and stress, adaptation to both oxidative and osmotic stress, and desiccation tolerance and drought stress. Volume Two covers heat shock responses, chaperonins and stress, cold shock responses, adaptation to acid stress, nitrosative stress, and envelope stress, as well as iron homeostasis, metal resistance, quorum sensing, chemotaxis and biofilm formation, and viable but not culturable (VBNC) cells. Covering the full breadth of current stress and environmental control of gene expression studies and expanding it towards future advances in the field, these two volumes are a one-stop reference for (non) medical molecular geneticists interested in gene regulation under stress.

Book Regulation of Transcriptional Elongation in Escherichia Coli

Download or read book Regulation of Transcriptional Elongation in Escherichia Coli written by Rachel Anne Mooney and published by . This book was released on 2007 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: