Download or read book Structure and Expression of the 6S RNA Operon of Escherichia Coli written by Zhuang Wang and published by . This book was released on 1985 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of Gene Expression in Escherichia coli written by E. C. C. Lin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 1010 pages. Available in PDF, EPUB and Kindle. Book excerpt: This up-to-date guide focuses on the understanding of key regulatory mechanisms governing gene expression in Escherichia coli. Studies of E. coli not only provide the first models of gene regulation, but research continues to yield different control mechanisms.

Download or read book Structure and Expression of a Complex Operon written by Timothy Allen Hoover and published by . This book was released on 1987 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structure and Expression of the Escherichia Coli Tryptophanyl tRNA Synthetase Operon written by Carol Virginia Hall and published by . This book was released on 1982 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structure and Expression of the Escherichia Coli Operons Containing Protein Synthesis Initiation Factor Genes InfA and InfB written by John Frederick Sands and published by . This book was released on 1986 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Decomposing the Symphony of Escherichia Coli Gene Expression written by Anand Varun Sastry and published by . This book was released on 2020 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteria respond and adapt to dynamic environments by altering their gene expression through a complex Transcriptional Regulatory Network (TRN). Advances in sequencing technologies have accelerated the generation of large RNA sequencing datasets that can be leveraged to probe the TRN. Here, we analyze large gene expression datasets using Independent Component Analysis (ICA), an unsupervised machine learning algorithm developed to separate source signals (e.g. individual instruments) from a set of mixed signals (e.g. recordings of an orchestra). First, we compile a high-quality RNA-seq compendium containing over 250 expression profiles for the model bacteria Escherichia coli. We apply ICA to decompose this compendium into independently modulated sets of genes, termed i-modulons, that represent the source signals comprising the transcriptome. Of the 92 i-modulons, 61 capture the genome-wide targets of characterized transcriptional regulators with high accuracy. ICA simultaneously estimates the activity of each transcriptional regulator in each growth condition. We show that this representation of the TRN can be used to predict and validate new regulatory interactions, characterize mutations in transcription factors, and provide a basis to compare gene expression across multiple E. coli strains. Next, we show that the underlying structure of the E. coli transcriptome, as determined by ICA, is conserved across multiple independent RNA-seq and microarray datasets. We subsequently combine the datasets into a compendium containing over 800 expression profiles, and discover that the ICA-based structure is still maintained upon data integration. Echoes of this structure were also found in two proteomics datasets, accelerating biological discovery through multi-omics analysis. Finally, we investigate how gene expression links environmental conditions to antibiotic efficacy. We show that i-modulons simplify the analysis of complex transcriptional changes to enable rapid characterization of cellular states beyond the transcriptome. As a whole, this body of work introduces ICA as a compelling tool to integrate and understand large gene expression datasets.

Download or read book Structural and Transcriptional Features of the Tryptophanase Operon of Escherichia Coli written by Michael Craig Deeley and published by . This book was released on 1982 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structure and Expression of the ampC lactamase Operon of escherichia Coli K 12 written by Thomas Grundström and published by . This book was released on 1981 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structure and expression of the manXYZ operon of Escherichia coli written by Per Saris and published by . This book was released on 1988 with total page 75 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structure and Expression of the ATP Synthase Operon of Escherichia Coli written by Jørgen Nielsen and published by . This book was released on 1984 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of Expression of the Escherichia Coli TRNA tufB Operon written by Joseph H. van Delft and published by . This book was released on 1987 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Features Involved in the Expression of Ribosomal RNA Operons in Escherichia Coli written by Karen L. Berg and published by . This book was released on 1988 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structure and Expression of the ampC lactamase Operon of escherichia Coli K 12 written by Thomas Grundström and published by . This book was released on 1981 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structure and Expression of the AmpC Beta lactamase Operon of Escherichia Coli K 12 written by Thomas Grundström and published by . This book was released on 1981 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book In Vitro Studies on the Regulation of Gene Expression in RNA Polymerase Operons of Escherichia Coli written by Susan Louise Peacock and published by . This book was released on 1985 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Nucleic Acids written by Victor A. Bloomfield and published by Sterling Publishing Company. This book was released on 2000-04-17 with total page 854 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing a comprehensive account of the structures and physical chemistry properties of nucleic acids, with special emphasis on biological function, this text has been organized to meet the needs of those who have only a basic understanding of physical chemistry and molecular biology.

Download or read book Heterologous Gene Expression in E coli written by Nicola A. Burgess-Brown and published by . This book was released on 2017 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.