Download or read book Structural and Functional Study of Bovine Herpesvirus 1 Glycoprotein B in the Interaction with Madin Darby Bovine Kidney Cells written by and published by . This book was released on 1901 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Entry of herpesviruses is mediated by the interactions between viral glycoproteins and cellular receptors. Among these glycoproteins, gB plays an important role. In this study, my major focus was to study gB's functions in the virus entry process and the structural requirements for gB to conduct its functions. The virus model in my study is bovine herpesvirus 1 (BHV-1), a member of the alphaherpesviruses. BHV-1 gB is a type I integral membrane protein with a potential transmembrane anchor at the C-terminal region. A cleavage site in the middle divides this molecule into two subunits, gBb and gBc. In this study, a truncated gB, gBt (residues 1 to 763), and N-terminal subunit, gBb (residues 1 to 505), were first expressed under the control of the bovine heat-shock protein 70A (hsp70A) gene promoter in stably transfected Madin Darby bovine kidney (MDBK) cells. Both forms of gB were secreted into the medium with apparent molecular weights as anticipated, and they were reactive to all gB-specific monoclonal antibodies used in this study. Affinity-purified gBt and gBb were able to elicit antibody responses in mice to an extent comparable to those induced by authentic gB. These results suggest that gBt and gBb retain the structural and antigenic properties of authentic gB. Furthermore, the intracellular processing of gBt and gBb was similar to that of authentic gB in virus-infected cells. Finally, gBt was proteolytically cleaved after conversion of the high mannose-containing precursor to the mature form. These truncated gBs that were prepared served as reagents for the core of my studies. BHV-I gB can bind to heparin sulfate (HS) and another non-HS receptor on MDBK cells. We assume that high-affinity binding to the non-HS receptor is important for BHV-1 infectivity. BHV-1 gB forms dimers in infected cells and in virions, and its dimerization domain may be located between residues 506 to 763. The cytoplasmic domain of BHV-1 gB is important for the existence of the high-aff.

Download or read book Structural and Functional Study of Bovine Herpesvirus 1 Glycoprotein B in the Interaction with Madin Darby Bovine Kidney Cells written by and published by . This book was released on 1996 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Entry of herpesviruses is mediated by the interactions between viral glycoproteins and cellular receptors. Among these glycoproteins, gB plays an important role. In this study, my major focus was to study gB's functions in the virus entry process and the structural requirements for gB to conduct its functions. The virus model in my study is bovine herpesvirus 1 (BHV-1), a member of the alphaherpesviruses. BHV-1 gB is a type I integral membrane protein with a potential transmembrane anchor at the C-terminal region. A cleavage site in the middle divides this molecule into two subunits, gBb and gBc. In this study, a truncated gB, gBt (residues 1 to 763), and N-terminal subunit, gBb (residues 1 to 505), were first expressed under the control of the bovine heat-shock protein 70A (hsp70A) gene promoter in stably transfected Madin Darby bovine kidney (MDBK) cells. Both forms of gB were secreted into the medium with apparent molecular weights as anticipated, and they were reactive to all gB-specific monoclonal antibodies used in this study. Affinity-purified gBt and gBb were able to elicit antibody responses in mice to an extent comparable to those induced by authentic gB. These results suggest that gBt and gBb retain the structural and antigenic properties of authentic gB. Furthermore, the intracellular processing of gBt and gBb was similar to that of authentic gB in virus-infected cells. Finally, gBt was proteolytically cleaved after conversion of the high mannose-containing precursor to the mature form. These truncated gBs that were prepared served as reagents for the core of my studies. BHV-I gB can bind to heparin sulfate (HS) and another non-HS receptor on MDBK cells. We assume that high-affinity binding to the non-HS receptor is important for BHV-1 infectivity. BHV-1 gB forms dimers in infected cells and in virions, and its dimerization domain may be located between residues 506 to 763. The cytoplasmic domain of BHV-1 gB is important for the existence of the high-aff.

Download or read book A Structural and Functional Analysis of Herpes Simplex Virus Type 1 Glycoprotein L an Essential Component of the Viral Fusogenic Complex written by Michael J. Novotny and published by . This book was released on 1996 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:

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Download or read book The Journal of Cell Biology written by and published by . This book was released on 2006 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: No. 2, pt. 2 of November issue each year from v. 19 (1963)-47 (1970) and v. 55 (1972)- contain the Abstracts of papers presented at the Annual Meeting of the American Society for Cell Biology, 3d (1963)-10th (1970) and 12th (1972)-

Download or read book Index Veterinarius written by and published by . This book was released on 1995 with total page 1630 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Virus Infections of Ruminants written by Z. Dinter and published by Elsevier. This book was released on 1990 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hardbound. As its title suggests, this book is intended to give exhaustive information on virus infections and diseases occurring in ruminants. Each virus and corresponding disease is described in a separate chapter, and the chapters are ordered according to current virus classification. Contributing authors are experts on the virus and disease they describe. Where necessary, the text is accompanied by tables and pictures. The accent is placed on diagnosis of the disease by clinical observations including epizootological data. There is also a description of the laboratory procedures of diagnostic virology, and suggestions concerning control measures (e.g. vaccination and/or eradication policy).This book should prove indispensable to: the practising veterinarian, by facilitating recognition of a viral disease, and giving advice on collecting appropriate specimens for laboratory investigation; the veterinary virologist by aiding the choice of appropriate

Download or read book Polymicrobial Diseases written by Kim A. Brogden and published by . This book was released on 2002 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polymicrobial diseases, those involving more than one etiologic agent, are more common than is generally realized and include respiratory diseases, gastroenteritis, conjunctivitis, keratitis, hepatitis, periodontal diseases, multiple sclerosis, genital infections, intra -- abdominal infections, and pertussis.

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Download or read book Herpesvirus Latency written by Benedikt B. Kaufer and published by Frontiers Media SA. This book was released on 2020-09-14 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Download or read book Lee V Hall written by and published by . This book was released on 1995 with total page 46 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Proceedings of the National Academy of Sciences of the United States of America written by National Academy of Sciences (U.S.) and published by . This book was released on 1984-11 with total page 1292 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Proceedings of the National Academy of Sciences (PNAS) publishes research reports, commentaries, reviews, colloquium papers, and actions of the Academy. PNAS is a multidisciplinary journal that covers the biological, physical, and social sciences.