Download or read book Structural and Functional Analysis of the Kaposi s Sarcoma associated Herpesvirus VFLIP Internal Ribosome Entry Site written by Mariam K. Sulaiman and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterizing a Unique IRES in Kaposi s Sarcoma Associated Herpesvirus written by Eileen Thuc Duyen Tran and published by . This book was released on 2016 with total page 63 pages. Available in PDF, EPUB and Kindle. Book excerpt: The synthesis of proteins during eukaryotic translation involves four major phases, where initiation is often the rate-limiting step and target for translational control with a large number of factors involved. Some viruses have evolved a mechanism that allows them to bypass the canonical initiation, with an internal ribosome entry site (IRES). IRESs can functionally replace the function of the 5' mRNA cap structure and many of the proteins that are involved in recruiting the ribosome. Found in Kaposi's sarcoma-associated herpesvirus, the vFLIP IRES is a unique IRES in that it is found in a DNA virus, within the coding region of a protein, and requires most of the host factors involved in translation initiation. To further characterize this IRES, an in vitro transcription approach was developed in order to generate milligram amounts of RNA required for crystallography. X-ray diffraction and structure determination of IRES crystals would lead to important insight into the viral IRES's mechanism of action. Although amounts sufficient for crystallography were not obtained during this work, optimization of the transcription approach showed that the RNA synthesis reactions would be amenable for scaling up. Secondly, a bicistronic reporter was developed suitable for functional investigations of the viral IRES in an in vitro translation assay as well as screening and testing of compounds for selective inhibitor of viral translation initiation.
Download or read book Kaposi Sarcoma Herpesvirus written by Department of Business Management C Boshoff and published by Springer. This book was released on 2016-04-01 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Elucidation of a Proteolytic Mechanism Required for Kaposi s Sarcoma associated Herpesvirus Replication written by Alan Brown Marnett and published by . This book was released on 2005 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpesviruses represent one of the most prevalent human pathogens worldwide. Infection results in a number of diseases ranging from cold sores to cancer. Yet despite the large clinical variation in disease states, the proteins expressed during the lytic cycle of all herpesviruses are highly conserved. A maturational serine protease is expressed late in viral replication and is required for production of properly assembled infectious progeny. In vitro structural and functional analysis has revealed the enzyme possesses a novel protein fold and is activated by dimerization. However, the mechanism of regulation of the enzyme both at a molecular level and in the context of viral replication is unknown. Positional scanning synthetic combinatorial libraries were used to define the substrate specificity of the protease encoded by Kaposi's Sarcoma-associated herpesvirus (KSHV). The strict specificity observed was used to guide the design and synthesis of a peptidyl-diphenylphosphonate inhibitor targeting the active site of the enzyme. Covalent modification of the active site serine of the enzyme by the transition-state analog inhibitor dramatically shifted the equilibrium toward dimeric enzyme illustrating the intimate communication between the spatially separate active sites and dimer interface of the protease. To elucidate the mechanism of stabilization and activation during catalysis, structural analysis was performed on purely monomeric (M197D), purely dimeric (inhibited) and an equilibrium mixture (uninhibited wild-type) by circular dichroism and nuclear magnetic resonance. A massive conformational change was observed upon loss of dimerization in which helices 5 and 6, at the interface and active site, respectively, become disordered, which destabilizes essential catalytic components. A disulfide bond was engineered into the protease at helix 6 that prevented the conformational change and subsequent inactivation of the enzyme. Addition of oxidant or reductant reversibly recovered or abolished enzymatic activity, respectively, even in a monomeric variant of the protease. A reduction in viral replication similar to currently available therapies targeting the viral polymerase was observed upon treatment of reactivated KSHV with the protease inhibitor. The work not only validated the virally-encoded protease as a potential therapeutic target, but also presented a mechanism by which protease inhibition may occur in vivo .
Download or read book Structure function Analysis of the Constitutive Activity of Kaposi s Sarcoma associated Herpesvirus G Protein coupled Receptor KSHV GPCR written by Hao H. Ho and published by . This book was released on 2002 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Functional Analysis of the Kaposi s Sarcoma associated Herpesvirus G Protein coupled Receptor written by and published by . This book was released on 2007 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Functional Analysis of Kaposi s Sarcoma associated Herpesvirus ORF57 RNA Binding Motifs written by Adam Taylor and published by . This book was released on 2011 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Kaposi Sarcoma New Insights for the Healthcare Professional 2011 Edition written by and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 62 pages. Available in PDF, EPUB and Kindle. Book excerpt: Kaposi Sarcoma: New Insights for the Healthcare Professional: 2011 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about Kaposi Sarcoma in a concise format. The editors have built Kaposi Sarcoma: New Insights for the Healthcare Professional: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Kaposi Sarcoma in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Kaposi Sarcoma: New Insights for the Healthcare Professional: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Kaposi Sarcoma Herpesvirus New Perspectives written by Chris Boshoff and published by Springer. This book was released on 2006-10-13 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: will follow
Download or read book Functional Analysis of the Latent Origin for DNA Replication of Kaposi s Sarcoma associated Herpesvirus written by Jianhong Hu and published by . This book was released on 2005 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Functional Analysis of Micro RNSa Encoded by Kaposi s Sarcoma associated Herpesvirus written by Mark Atienza Samols and published by . This book was released on 2007 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Translational Control of Kaposi s Sarcoma Associated Herpesvirus KSHV VFlip Expression written by Zulkefley Othman and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA Therapeutics written by Amanda L. Garner and published by Springer. This book was released on 2017-09-26 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medicinal chemistry is both science and art. The science of medicinal chemistry offers mankind one of its best hopes for improving the quality of life. The art of medicinal chemistry continues to challenge its practitioners with the need for both intuition and experience to discover new drugs. Hence sharing the experience of drug research is uniquely beneficial to the field of medicinal chemistry. Drug research requires interdisciplinary team-work at the interface between chemistry, biology and medicine. Therefore, the topic-related series Topics in Medicinal Chemistry covers all relevant aspects of drug research, e.g. pathobiochemistry of diseases, identification and validation of (emerging) drug targets, structural biology, drugability of targets, drug design approaches, chemogenomics, synthetic chemistry including combinatorial methods, bioorganic chemistry, natural compounds, high-throughput screening, pharmacological in vitro and in vivo investigations, drug-receptor interactions on the molecular level, structure-activity relationships, drug absorption, distribution, metabolism, elimination, toxicology and pharmacogenomics. In general, special volumes are edited by well known guest editors.
Download or read book Functional Analysis of the Latency associated Nuclear Antigen of Kaposi s Sarcoma associated Herpes Virus written by Alexander Charles Garber and published by . This book was released on 2002 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Herpesviruses written by Ann Arvin and published by Cambridge University Press. This book was released on 2007-08-16 with total page 1325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2002 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book The Journal of Immunology written by and published by . This book was released on 2004 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt:
