Download or read book Stabilizing Protein Function written by Ciarán ÓFágáin and published by Springer. This book was released on 1997-10-17 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an account of our current knowledge of protein stability. It demonstrates how an understanding of protein stability has emerged from investigations of protein denaturation/inactivation and from studies on stabilizing interactions in proteins. It also describes the stability characteristics of enzymes in nonaqueous media and the special adaptations of thermophilic proteins. Manipulation of protein stability is outlined in chapters on the use of additives, chemical modification of proteins in solution, immobilization, protein engineering and long-term storage of proteins. The book includes numerous case studies and copious references to original research papers and relevant reviews and monographs.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Stability and Stabilization Through Protein Engineering written by Yoshiaki Nosoh and published by Ellis Horwood. This book was released on 1991 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Engineering of Thermally Stable Proteins and Photo switchable Proteins written by Fuzhong Zhang and published by . This book was released on 2009 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this project is to develop general approaches to the control of protein structures and functions. The project mainly consists of three aspects: (1) stabilizing folded protein structures, (2) reversible photo-control of protein folding and function, (3) design of a photo-switchable dominant negative protein to photo-control DNA binding.(1) Most proteins adopt specific folded structures to perform biological functions. Stabilizing the active folded forms of peptides or proteins is thus important for maintaining or enhancing the functions of these molecules. We hypothesized that a protein's folded structure could be stabilized by introduction of a rigid cross-linker with its length matching the distance of the two attachment points in the folded structure. To test this, we synthesized a thiol-reactive alkyne-based rigid cross-linker and tested its effect. When it was introduced at i and i+11 positions of a model alpha-helical peptide, a significant promotion in the folded structure as well as strong resistance against thermal melting was observed. The rigid cross-linker was also applied to the Fyn SH3 domain, a protein with tertiary structure and a similar stabilization effect was obtained. This work demonstrates that a rigid cross-linker can be generally used to stabilize folded peptide/protein structures.(3) The third aspect of my PhD research is to apply the photo-switchable proteins to photo-control of Jun/Fos DNA binding activity. Fos and Jun are important transcription factors implicated in numerous cancers. They form a hetero-dimer that binds to specific DNA sequences. Dominant negative proteins are mutants of Fos or Jun that prevent native Jun/Fos DNA binding activity. I designed photo-switchable versions of these dominant negative proteins by covalently introducing photo-switchable cross-linkers. These proteins do not have any function in the dark due to their disrupted structures induced by the trans form cross-linkers. They only function as dominant negative proteins when the cross-linker is in the cis form after photo-irradiation. Several such proteins were synthesized and their effectiveness was tested. These photo-switchable dominant negative proteins are powerful tools for temporal control of Jun/Fos regulated gene expression.(2) Reversible photo-switch of protein folding/unfolding offers exciting prospects for external manipulation of protein function because of its fast response, high spatial resolution and compatibility with living systems. I developed a general approach to the design of photo-switchable proteins based on the introduction of photo-switchable intramolecular cross-linkers. An azobenzene based photo-switch was used because the energy available from photoisomerization is higher than the free energy of protein folding. I chose the FynSH3 domain as a model protein. Taking the experimentally determined structure of the folded protein as a starting point, mutations were made to introduce pairs of Cys residues so that the distance between Cys sulfur atoms matches the ideal length of the cis form, but not the trans form, of the cross-linker. When the L3C-L29C-T47AFynSH3 mutant was cross-linked with the trans cross-linker, the protein was destabilized so that folded and unfolded forms coexisted. Irradiation of the cross-linker to produce the cis isomer recovered the folded state of the protein. Photo-control of FynSH3 binding to a proline-rich peptide was also demonstrated. This work shows that structure-based introduction of switchable cross-linkers is a feasible general approach for photo-control of global folding/unfolding of globular proteins, and thereby photo-control of their activity.
Download or read book Protein Stability written by David S. Eisenberg and published by . This book was released on 1995 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: The topics covered by this volume include: protein destabilization at low temperatures; engineering the stability and function of Gene V Protein; free energy balance in protein folding; modelling protein stability as a heteropolymer collapse; stability of alpha helices; protein stability with T4 Lysozyme.
Download or read book Protein Biotechnology written by Felix Franks and published by Springer Science & Business Media. This book was released on 2008-02-22 with total page 594 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are the servants of life. They occur in all com- nent parts of living organisms and are staggering in their fu- tional variety, despite their chemical similarity. Even the simplest single-cell organism contains a thousand different p- teins, fulfilling a wide range of life-supporting roles. Additions to the total number of known proteins are being made on an increasing scale through the discovery of mutant strains or their production by genetic manipulation. The total international protein literature could fill a medi- sized building and is growing at an ever-increasing rate. The reader might be forgiven for asking whether yet another book on proteins, their properties, and functions can serve a useful purpose. An explanation of the origin of this book may serve as justification. The authors form the tutorial team for an int- sive postexperience course on protein characterization or- nized by the Center for Professional Advancement, East Brunswick, New Jersey, an educational foundation. The course was first mounted in Amsterdam in 1982 and has since been repeated several times, in both Amsterdam and the US, with participants from North America and most European countries. In a predecessor to this book, emphasis was placed on the role of protein isolation in the food industry, because at the time this reflected the interests of most of the participants at the course. Today, isolated proteins for food use are extracted from yeasts, fungal sources, legumes, oilseeds, cereals, and leaves.
Download or read book Conditional Control of Protein Function written by Lystranne Alysia Maynard and published by . This book was released on 2006 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Effects of Denaturing and Stabilizing Solutes on Protein Stability Function and Internal Dynamics written by Aijun Wang and published by . This book was released on 1995 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Function written by Thomas E. Creighton and published by Oxford University Press, USA. This book was released on 1989 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Rational Design of Stable Protein Formulations written by John F. Carpenter and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant proteins and polypeptides continue to be the most important class of biotechnology-derived agents in today's pharmaceutical industry. Over the past few years, our fundamental understanding of how proteins degrade and how stabilizing agents work has made it possible to approach formulation of protein pharmaceuticals from a much more rational point of view. This book describes the current level of understanding of protein instability and the strategies for stabilizing proteins under a variety of stressful conditions.
Download or read book Protein Structure Stability and Folding written by Kenneth P. Murphy and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Protein Structure, Stability, and Folding, Kenneth P. Murphy and a panel of internationally recognized investigators describe some of the newest experimental and theoretical methods for investigating these critical events and processes. Among the techniques discussed are the many methods for calculating many of protein stability and dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches presented include the use of co-solvents, novel applications of hydrogen exchange techniques, temperature-jump methods for looking at folding events, and new strategies for mutagenesis experiments. Unique in its powerful combination of theory and practice, Protein Structure, Stability, and Folding offers protein and biophysical chemists the means to gain a more comprehensive understanding of some of this complex area by detailing many of the major techniques in use today.
Download or read book The Role of Water in Protein Structure Stabilization written by Julia Josovitz and published by . This book was released on 2003 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Bioinformatics written by M. Michael Gromiha and published by Academic Press. This book was released on 2011-04-21 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the most pressing tasks in biotechnology today is to unlock the function of each of the thousands of new genes identified every day. Scientists do this by analyzing and interpreting proteins, which are considered the task force of a gene. This single source reference covers all aspects of proteins, explaining fundamentals, synthesizing the latest literature, and demonstrating the most important bioinformatics tools available today for protein analysis, interpretation and prediction. Students and researchers of biotechnology, bioinformatics, proteomics, protein engineering, biophysics, computational biology, molecular modeling, and drug design will find this a ready reference for staying current and productive in this fast evolving interdisciplinary field. Explains all aspects of proteins including sequence and structure analysis, prediction of protein structures, protein folding, protein stability, and protein interactions Presents a cohesive and accessible overview of the field, using illustrations to explain key concepts and detailed exercises for students.
Download or read book Proteins written by James J. L'Italien and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume surveys the current status of many of the important methods and approaches which are central to the study of protein structure and function. Many of the articles in this volume are written to emphasize the general utility of the method or approach which is at its core, and to provide sufficient literature references to enable the reader to adapt the method or approach to other applications. It is hoped that this volume will provide a source from which newcomers as well as experienced scient ists may becom& more familiar with recent developments and future trends in some of the important areas of protein research. The articles which comprise this book are selected proceedings from the Symposium of American Protein Chemists, which was held in San Diego, California, September 30 to October 3, 1985. The goal of the organizers of this first symposium was to provide a forum for discussion and inter action among scientists whose interests span the broad spectrum of protein structure and function research. The concept and timing of the symposium well received as evidenced by the approximately 500 delegates to the was symposium. The inaugural meeting was marked by a strong scientific pro gram with over 140 papers presented in either a lecture or poster format.
Download or read book Biology for AP Courses written by Julianne Zedalis and published by . This book was released on 2017-10-16 with total page 1923 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biology for AP® courses covers the scope and sequence requirements of a typical two-semester Advanced Placement® biology course. The text provides comprehensive coverage of foundational research and core biology concepts through an evolutionary lens. Biology for AP® Courses was designed to meet and exceed the requirements of the College Board’s AP® Biology framework while allowing significant flexibility for instructors. Each section of the book includes an introduction based on the AP® curriculum and includes rich features that engage students in scientific practice and AP® test preparation; it also highlights careers and research opportunities in biological sciences.
Download or read book Drying Technologies for Biotechnology and Pharmaceutical Applications written by Satoshi Ohtake and published by John Wiley & Sons. This book was released on 2020-06-02 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive source of information about modern drying technologies that uniquely focus on the processing of pharmaceuticals and biologicals Drying technologies are an indispensable production step in the pharmaceutical industry and the knowledge of drying technologies and applications is absolutely essential for current drug product development. This book focuses on the application of various drying technologies to the processing of pharmaceuticals and biologicals. It offers a complete overview of innovative as well as standard drying technologies, and addresses the issues of why drying is required and what the critical considerations are for implementing this process operation during drug product development. Drying Technologies for Biotechnology and Pharmaceutical Applications discusses the state-of-the-art of established drying technologies like freeze- and spray- drying and highlights limitations that need to be overcome to achieve the future state of pharmaceutical manufacturing. The book also describes promising next generation drying technologies, which are currently used in fields outside of pharmaceuticals, and how they can be implemented and adapted for future use in the pharmaceutical industry. In addition, it deals with the generation of synergistic effects (e.g. by applying process analytical technology) and provides an outlook toward future developments. -Presents a full technical overview of well established standard drying methods alongside various other drying technologies, possible improvements, limitations, synergies, and future directions -Outlines different drying technologies from an application-oriented point of view and with consideration of real world challenges in the field of drug product development -Edited by renowned experts from the pharmaceutical industry and assembled by leading experts from industry and academia Drying Technologies for Biotechnology and Pharmaceutical Applications is an important book for pharma engineers, process engineers, chemical engineers, and others who work in related industries.
Download or read book The Role of Flexibility and Protein protein Interactions in the Function of the Manganese Stabilizing Protein of Photosystem II written by Adele Kim Williamson and published by . This book was released on 2008 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt:
