Download or read book Spheroids in Cancer Research written by H. Acker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malignant growth of cells is often characterized by disorganization of tissue structure, abnormal blood vessel development, and insuffi cient vascular supply. As a consequence, the cancer cells grow in a three-dimensional pattern in atypical microenvironments which include physical, chemical, and nutritional stresses. Necrosis often develops some distance away from the blood vessels. In association with an inherent instability in malignant cell populations, and also because of the changing micromilieu, significant cellular heteroge neity emerges with regard to various phenotypic characteristics. Both biological behavior and responses to therapeutic agents can be affected. A variety of in vitro and in vivo experimental models exist for research on properties of cancer cells during growth. The multicell spheroid model was developed as a system of intermediate complexity in which three dimensional growth of cells enhances cell-cell interactions and creates micro environments that simulate the conditions in intervascular microregions of tumors or microme tastatic foci. Spheroids may change their cellular characteristics with changing environments during growth. These can be studied under controlled conditions in vitro. Interest in details of experimental methods for this model system stimulated the organization of the First International Conference in Rochester, NY in 1980, the Proceedings of which were summarized in Cancer Research in 1981. Since then there has been a rapid increase in the use of this model system, and increased research on the significance of cell-cell and cell-microenvironment interactions in biology in general.
Download or read book Spheroid Culture in Cancer Research 1991 written by Rolf Bjerkvig and published by CRC Press. This book was released on 2017-11-22 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spheroid Culture in Cancer Research describes the various techniques now available for establishing spheroid tissue culture, including spheroid culture from normal tissues and from tumor cell lines. The book also describes how the spheroid system can be used to study interactions between normal and malignant cells. Microenvironmental conditions in spheroids and how this micromilieu may promote cellular heterogeneity and histiotypic structures not observed in corresponding monolayer cultures are discussed. The biological importance of oxygen tension, pH gradients, diffusions of nutrients, and cell-cell communication in spheroids are also examined. The book will be profoundly important to researchers in experimental chemotherapy, radiotherapy, immunotherapy, and hyperthermia.
Download or read book Spheroid Culture in Cancer Research written by Rolf Bjerkvig and published by CRC-Press. This book was released on 1991-11-19 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spheroid Culture in Cancer Research describes the various techniques now available for establishing spheroid tissue culture, including spheroid culture from normal tissues and from tumor cell lines. The book also describes how the spheroid system can be used to study interactions between normal and malignant cells. Microenvironmental conditions in spheroids and how this micromilieu may promote cellular heterogeneity and histiotypic structures not observed in corresponding monolayer cultures are discussed. The biological importance of oxygen tension, pH gradients, diffusions of nutrients, and cell-cell communication in spheroids are also examined. The book will be profoundly important to researchers in experimental chemotherapy, radiotherapy, immunotherapy, and hyperthermia.
Download or read book Three dimensional Spheroid Culture written by Bhawani Aryasomayajula and published by . This book was released on 2012 with total page 37 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monolayer cultures have been the preferred method for testing the activity of anticancer drug candidates in vitro. However, cell based assays performed in monlayer culture provides data from cells in different state lacking many of the characteristics of the tumor environment such as the necrotic core, hypoxic region and low pH at core. A novel approach to overcome the drawbacks of monolayer cultures is the three-dimensional in vitro culture also knows as multi-cellular tumor spheroids or Spheroid culture or three-dimensional culture. Micro tumor tissues produced by spheroid culture helps to mimic many of the characteristics of tumor environment as well as helps to produce data more relevant to in vivo tumors. In this report, we optimized various techniques of culturing spheroids and worked towards making spheroid culture quick, accurate, and high-throughput. Two methods of spheroid culture were examined, non-adhesive liquid overlay with and without Reconstituted Basement Membrane (RBM / Matrigel) and hanging drop method. Non-adhesive liquid overlay without RBM was found to be the most effective. These spheroids were consistent with respect to size, shape and cell number. The spheroids generated by this method were utilized to evaluate cancer cell behavior in three dimension. To determine the cytotoxicity of paclitaxel in spheroids the spheroid cultures after treatment with a drug, the activity of phosphatase enzyme, using pNPP as the substrate, was measured. The cytotoxicity of paclitaxel in spheroids was then compared to the cytotoxicity in monolayer cultures of A549 cells. The results showed significant reduction in cytotoxicity of paclitaxel on A549 cells when treated in spheroid culture compared to monolayer cultures. Three-dimensional spheroids of A549 cells did demonstrate inherent drug resistance demonstrated by many in vivo tumors . The reduced cytotoxicity of paclitaxel could be due to inhibited drug penetration resulting from cell-cell interaction and presence of cells in different cell cycle stages. It is, therefore, cell cycle analysis was carried out on A549 cells grown as spheroids and compared with cells in monolayer culture using propidium iodide. Results indicated presence of significantly higher population in G0/G1 phase and reduced population in S phase in spheroids compared to monolayer culture. Based on the results obtained, we believe use of spheroids in cancer research could provide a unique platform to conduct previously unfeasible experiments and provide valuable insights for cancer cell behavior in tissue like structure.
Download or read book Revival Spheroid Culture in Cancer Research 1991 written by Rolf Bjerkvig and published by CRC Press Revivals. This book was released on 2019-01-28 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spheroid Culture in Cancer Research describes the various techniques now available for establishing spheroid tissue culture, including spheroid culture from normal tissues and from tumor cell lines. The book also describes how the spheroid system can be used to study interactions between normal and malignant cells. Microenvironmental conditions in spheroids and how this micromilieu may promote cellular heterogeneity and histiotypic structures not observed in corresponding monolayer cultures are discussed. The biological importance of oxygen tension, pH gradients, diffusions of nutrients, and cell-cell communication in spheroids are also examined. The book will be profoundly important to researchers in experimental chemotherapy, radiotherapy, immunotherapy, and hyperthermia.
Download or read book Tumor Microenvironment written by Alexander Birbrair and published by Springer Nature. This book was released on 2020-02-06 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses recent research advances in cancer biology, focusing on the role of the tumor microenvironment. Taken alongside its companion volumes, Tumor Microenvironment: Recent Advances covers the latest research on various aspects of the tumor microenvironment, as well as future directions. Useful for introducing the newer generation of researchers to the history of how scientists studied the tumor microenvironment as well as how this knowledge is currently applied for cancer treatments, it will be essential reading for advanced cell biology and cancer biology students, as well as researchers seeking an update on research on the tumor microenvironment.
Download or read book Spheroids in Cancer Research written by and published by . This book was released on 1987 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Unraveling the Safety Profile of Nanoscale Particles and Materials written by Andreia Ferreira de Castro Gomes and published by BoD – Books on Demand. This book was released on 2018-03-21 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: As nanomaterials become increasingly present in our daily lives, pertinent questions regarding their safety arise. Nanomaterial risk assessment, as in other areas, directs much of the effort worldwide in defining guidelines that may be translated into national or international directives. Nanomaterials encompass different entities, from nanoparticles to nanostructured materials, with specific effects over cells, tissues, organisms and ecosystems depending on their biophysical characteristics. Such interactions will directly affect the impact of novel nanotechnologies. This book aims to provide the reader with a comprehensive overview of the current state of the art in nanotoxicology, featuring the most important developments and critical issues regarding the use of and exposure to nanoparticles.
Download or read book Bio Nanomedicine for Cancer Therapy written by Flavia Fontana and published by Springer Nature. This book was released on 2021-02-04 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book covers the latest developments in biologically-inspired and derived nanomedicine for cancer therapy. The purpose of the book is to illustrate the significance of naturally-mimicking systems for enhancing the dose delivered to the tumor, to improve stability, and prolong the circulation time. Moreover, readers are presented with advanced materials such as adjuvants for immunostimulation in cancer vaccines. The book also provides a comprehensive overview of the current status of academic research. This is an ideal book for students, researchers, and professors working in nanotechnology, cancer, targeted drug delivery, controlled drug release, materials science, and biomaterials as well as companies developing cancer immunotherapy.
Download or read book Cancer Cell Culture written by Dania Movia and published by Springer Nature. This book was released on 2023-05-18 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest collection of cell models that are used in preclinical cancer research, and covers both two-dimensional and three-dimensional culturing techniques. The chapters in this book are divided into two parts. Part One discusses two-dimensional cancer cell culture, cell models at the Air-Liquid Interface, and the latest advancements in three-dimensional complex spheroid models and dedicated disease animal models. Part Two contains technical chapters that illustrate step-by-step methodologies for specific cancer cell culture methods. The methods discussed range from the generation of isogenic cancer cell lines, the use of serum-free growth conditions, and three-dimensional cell cultures and their specific assays for the efficacy assessment of new anticancer therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cancer Cell Culture: Methods and Protocols is a valuable tool to help researchers involved in this important field to further improve or advance their models for cancer research.
Download or read book Technology Platforms for 3D Cell Culture written by Stefan Przyborski and published by John Wiley & Sons. This book was released on 2017-03-03 with total page 667 pages. Available in PDF, EPUB and Kindle. Book excerpt: Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs. Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture Features contributions from leading developers and researchers active in 3D cell technology Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied Includes information on resources and technical support to help initiate the use of 3D culture methods
Download or read book Tissue Engineering written by Hansjörg Hauser and published by Springer Science & Business Media. This book was released on 2007-07-27 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Features: Leading experts present their own most recent advances, Includes a wide spectrum of methods representing tissue engineering in many diverse disciplines, Supplies an understanding of diverse technologies and methods.
Download or read book Preclinical Models and Emerging Technologies to Study the Effects of the Tumor Microenvironment on Cancer Heterogeneity and Drug Resistance written by Giulia Adriani and published by Frontiers Media SA. This book was released on 2023-10-26 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Million Cells in Search for Contact written by Dieter F. Hülser and published by . This book was released on 1987 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Tumor Organoids written by Shay Soker and published by Humana Press. This book was released on 2017-10-20 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer cell biology research in general, and anti-cancer drug development specifically, still relies on standard cell culture techniques that place the cells in an unnatural environment. As a consequence, growing tumor cells in plastic dishes places a selective pressure that substantially alters their original molecular and phenotypic properties.The emerging field of regenerative medicine has developed bioengineered tissue platforms that can better mimic the structure and cellular heterogeneity of in vivo tissue, and are suitable for tumor bioengineering research. Microengineering technologies have resulted in advanced methods for creating and culturing 3-D human tissue. By encapsulating the respective cell type or combining several cell types to form tissues, these model organs can be viable for longer periods of time and are cultured to develop functional properties similar to native tissues. This approach recapitulates the dynamic role of cell–cell, cell–ECM, and mechanical interactions inside the tumor. Further incorporation of cells representative of the tumor stroma, such as endothelial cells (EC) and tumor fibroblasts, can mimic the in vivo tumor microenvironment. Collectively, bioengineered tumors create an important resource for the in vitro study of tumor growth in 3D including tumor biomechanics and the effects of anti-cancer drugs on 3D tumor tissue. These technologies have the potential to overcome current limitations to genetic and histological tumor classification and development of personalized therapies.
Download or read book Physics Of Cancer The Research Advances written by Bernard S Gerstman and published by World Scientific. This book was released on 2020-12-03 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer deaths per capita have decreased in recent years, but the improvement is attributed to prevention, not treatment. The difficulty in treating cancer may be due to its 'complexity', in the mathematical physics sense of the word. Tumors evolve and spread in response to internal and external factors that involve feedback mechanisms and nonlinear behavior. Investigations of the nonlinear interactions among cells, and between cells and their environment, are crucial for developing a sufficiently detailed understanding of the system's emergent phenomenology to be able to control the behavior. In the case of cancer, controlling the system's behavior will mean the ability to treat and cure the disease. Physicists have been studying various complex, nonlinear systems for many years using a variety of techniques. These investigations have provided insights that allow physicists to make unique contributions towards the treatment of cancer.This interdisciplinary book presents recent advancements in physicists' research on cancer. The work presented in this volume uses a variety of physical, biochemical, mathematical, theoretical, and computational techniques to gain a deeper molecular and cellular understanding of the horrific disease that is cancer.
Download or read book Principles of Regenerative Medicine written by Anthony Atala and published by Academic Press. This book was released on 2010-12-16 with total page 1203 pages. Available in PDF, EPUB and Kindle. Book excerpt: Virtually any disease that results from malfunctioning, damaged, or failing tissues may be potentially cured through regenerative medicine therapies, by either regenerating the damaged tissues in vivo, or by growing the tissues and organs in vitro and implanting them into the patient. Principles of Regenerative Medicine discusses the latest advances in technology and medicine for replacing tissues and organs damaged by disease and of developing therapies for previously untreatable conditions, such as diabetes, heart disease, liver disease, and renal failure. Key for all researchers and instituions in Stem Cell Biology, Bioengineering, and Developmental Biology The first of its kind to offer an advanced understanding of the latest technologies in regenerative medicine New discoveries from leading researchers on restoration of diseased tissues and organs
