Download or read book Sperm mediated Gene Transfer written by Kevin Richard Smith and published by Bentham Science Publishers. This book was released on 2012 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Sperm-mediated gene transfer (SMGT) represents a novel set of technologies for animal (or in the future, human) genetic modification using the sperm as a vector, as opposed to more traditional established routes such as fertilized eggs or embryonic stem c"
Download or read book Sperm mediated Gene Transfer written by Kevin Richard Smith and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Efficiency of Rabbits Transgenesis by Sperm Mediated Gene Transfer written by Mohammed Baqur Sahib Al-Shuhaib and published by LAP Lambert Academic Publishing. This book was released on 2012-07 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: The feasibility of sperm cells to act literally as gene vehicles was discussed using one of the most modern techniques in this aspect which is known as restriction enzyme mediated integration sperm mediated gene transfer (REMI-SMGT)technique. Several objectives were pursued in this book; such as to test the validity of REMI-SMGT, to evaluate the efficiency of this technique in generating mammalian species rather than the sheep that made only by one group and to observe the possibility of doing so by using rabbits as a model for this approach. Add to that, it was aimed to reduce the cost of REMI-SMGT by substituting liposomes and highly cost effective media with a high efficient, non-cost effective substitute. Moreover, it was directed to identify if any band substitution was occurred by simple seminal proteins - exogenous DNA incubation by one gel double stained non-radioactive method to identify the role of seminal proteins charge on the nature of seminal fluid inhibitory effect that exerted against the invading DNA. This might help us to approach closely to the nature of inhibitory activities in seminal proteins that prevent the entry of foreign DNA into the head of the sperm.
Download or read book Sperm mediated Gene Transfer in Pigs written by University of Guelph. Department of Animal & Poultry Science and published by . This book was released on 2007 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Linker based Sperm Mediated Gene Transfer Method for the Production of Transgenic Rat written by Jeannie M. Epperly and published by . This book was released on 2007 with total page 66 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The purpose of the current research is to create a species-specific variation of the LB-SMGT [linker based sperm mediated gene transfer] method, perfecting it for the reproductive complexities of the rat. The areas studied involve; overcoming the intricacies of the female rat estrous cycle, as well as developing sperm treatments and efficiencies in the delivery of genes. An exogenous gene; EGFP (Enhanced Green Fluorescent Protein) was delivered to the genome of SHR/y rats via an adaptation of LB-SMGT method which produced a transgenic rate exhibiting GFP expression. Results of this research are to be determined by PCR that amplified a 235bp fragment of EGFP."--Page 14 [i.e. 6].
Download or read book Sperm Mediated Gene Transfer Using Non viral Episomal Vector written by Stefano Manzini and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proceedings of the Workshop Sperm Mediated Gene Transfer Advances in Sperm Cell Research and Applications written by Baccio Baccetti and published by . This book was released on 2000 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microinjection and Transgenesis written by Angel Cid-Arregui and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 619 pages. Available in PDF, EPUB and Kindle. Book excerpt: The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics.
Download or read book Sperm Mediated Gene Transfer for generation of transgenic jundi s Rhamdia quelen written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Várias metodologias de inserção de DNA exógeno em células eucarióticas vêmsendo estudadas nas últimas duas décadas. Em peixes, a técnica mais estudadaaté o momento é a microinjeção, que proporciona a inserção de fragmentosgênicos de interesse em zigotos. No entanto esta metodologia apresenta diversosfatores negativos: consome tempo e trabalho, exige qualificação técnica, háelevada mortalidade de embriões, a percentagem de integração é freqüentementebaixa, a opacidade dos ovos de peixes não permite a visualização do pró-núcleo, além da membrana coriônica ser muito resistente em muitas espécies de peixestornando-se uma barreira à microinjeção. Uma alternativa de otimização doprocesso de inserção de DNA exógeno, teoricamente consiste na transfecçãomassal de células espermáticas pré fertilização in vitro. A transferência gênicamediada por espermatozóides (SMGT) vem sendo utilizada no estudo e geraçãode diversas espécies de animais transgênicos, inclusive peixes. Algumasferramentas como eletroporação, diferencial osmótico e incubação espermáticasão alternativas que podem potencializar a SMGT em peixes. Este estudo teve porobjetivo avaliar o potencial espermático do jundiá (Rhamdia quelen) submetido adiferentes tratamentos de inserção de DNA exógeno para gerar jundiástransgênicos. Para atingir este objetivo utilizou-se dois vetores pCMV-GFP epcBA-GFP, linearizados, em 5 tratamentos propostos: desidratação/reidratação (DR); eletroporação (E); desidratação/reidratação/eletroporação (DRE);incubação com plasma seminal (INC) e incubação sem plasma seminal (INCSP).Como controle sêmen diluído em solução isotônica sem DNA exógeno. Paraavaliar o potencial espermático foram mensurados atividade espermática (AT), tempo de permanência em atividade (TPA), integridade de membrana efertilização in vitro (FIV) através das TXFIV (taxa de fertilização in vitro) e TXECLO(taxa de eclosão). Para avaliar a expressão da GFP os animais foramanestesiados e observados em microscopia de fluorescência. As análisesestatísticas demonstraram que houve efeito significativo (P
Download or read book Sustainable Food Production written by Paul Christou and published by Springer. This book was released on 2012-12-05 with total page 1869 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gathering some 90 entries from the Encyclopedia of Sustainability Science and Technology, this book covers animal breeding and genetics for food, crop science and technology, ocean farming and sustainable aquaculture, transgenic livestock for food and more.
Download or read book Animal Biotechnology written by National Research Council and published by National Academies Press. This book was released on 2002-12-29 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic-based animal biotechnology has produced new food and pharmaceutical products and promises many more advances to benefit humankind. These exciting prospects are accompanied by considerable unease, however, about matters such as safety and ethics. This book identifies science-based and policy-related concerns about animal biotechnologyâ€"key issues that must be resolved before the new breakthroughs can reach their potential. The book includes a short history of the field and provides understandable definitions of terms like cloning. Looking at technologies on the near horizon, the authors discuss what we know and what we fear about their effectsâ€"the inadvertent release of dangerous microorganisms, the safety of products derived from biotechnology, the impact of genetically engineered animals on their environment. In addition to these concerns, the book explores animal welfare concerns, and our societal and institutional capacity to manage and regulate the technology and its products. This accessible volume will be important to everyone interested in the implications of the use of animal biotechnology.
Download or read book Safety of Genetically Engineered Foods written by National Research Council and published by National Academies Press. This book was released on 2004-07-08 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
Download or read book Advanced Protocols for Animal Transgenesis written by Shirley Pease and published by Springer Nature. This book was released on 2011-08-30 with total page 671 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.
Download or read book Influence of Genomic DNA Transfer on Egyptian Chicken s Performance written by Osama El-Garhy and published by . This book was released on 2013-06-26 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Heritable Human Genome Editing written by The Royal Society and published by National Academies Press. This book was released on 2021-01-16 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: Heritable human genome editing - making changes to the genetic material of eggs, sperm, or any cells that lead to their development, including the cells of early embryos, and establishing a pregnancy - raises not only scientific and medical considerations but also a host of ethical, moral, and societal issues. Human embryos whose genomes have been edited should not be used to create a pregnancy until it is established that precise genomic changes can be made reliably and without introducing undesired changes - criteria that have not yet been met, says Heritable Human Genome Editing. From an international commission of the U.S. National Academy of Medicine, U.S. National Academy of Sciences, and the U.K.'s Royal Society, the report considers potential benefits, harms, and uncertainties associated with genome editing technologies and defines a translational pathway from rigorous preclinical research to initial clinical uses, should a country decide to permit such uses. The report specifies stringent preclinical and clinical requirements for establishing safety and efficacy, and for undertaking long-term monitoring of outcomes. Extensive national and international dialogue is needed before any country decides whether to permit clinical use of this technology, according to the report, which identifies essential elements of national and international scientific governance and oversight.
Download or read book Introduction to Pharmaceutical Biotechnology Volume 1 written by Saurabh Bhatia and published by . This book was released on 2018-05-23 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal biotechnology is a broad field including polarities of fundamental and applied research, as well as DNA science, covering key topics of DNA studies and its recent applications. In Introduction to Pharmaceutical Biotechnology, DNA isolation procedures followed by molecular markers and screening methods of the genomic library are explained in detail. Interesting areas such as isolation, sequencing and synthesis of genes, with broader coverage of the latter, are also described. The book begins with an introduction to biotechnology and its main branches, explaining both the basic science and the applications of biotechnology-derived pharmaceuticals, with special emphasis on their clinical use. It then moves on to the historical development and scope of biotechnology with an overall review of early applications that scientists employed long before the field was defined. Additionally, this book offers first-hand accounts of the use of biotechnology tools in the area of genetic engineering and provides comprehensive information related to current developments in the following parameters: plasmids, basic techniques used in gene transfer, and basic principles used in transgenesis. The text also provides the fundamental understanding of stem cell and gene therapy, and offers a short description of current information on these topics as well as their clinical associations and related therapeutic options.
Download or read book Caprine Sperm Cells as Vectors for Gene Transfer written by Yongxin Sun and published by . This book was released on 1997 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:
