Download or read book Spectrophotometry and Spectrofluorimetry written by Mike Gore and published by OUP Oxford. This book was released on 2000-05-04 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition was written with the intention to help the reader understand the background concepts and practical applications of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day to day operations of most laboratories in the chemical, biological and medical sciences and this edition contains substantially updated and new chapters addressing the principles of most of the more common applications such as: spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolved fluorescence and phosphorescence studies, circular dichrosim and pre-equilibrium spectroscopic techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Other chapters are included to introduce subjects that have traditionally depended upon spectroscopy such as basic enzyme kinetics, ligand binding, data handling and the more recently established interest in the study of protein and DNA stability. Finally, the concept of 'global analysis' is introduced to provide the reader with an insight into this method of utilizing the vast arrays of experimental data provided by current instrumentation.
Download or read book Spectrophotometry Spectrofluorimetry written by D. A. Harris and published by Oxford University Press, USA. This book was released on 1987 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using this book biochemists can determine how spectrophotometry can contribute to laboratory analyses. Emphasis is placed on the capabilities and limitations of the instrument in use--how to select a machine, how to check if it is working satisfactorily, and what to do if it fails to produce the data expected.
Download or read book Spectrophotometry and Spectrofluorimetry written by C. L. Bashford and published by . This book was released on 1987 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Spectrophotometry and Spectrofluorimetry written by Michael G. Gore and published by Oxford University Press, USA. This book was released on 2000 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book helps readers better understand the background concepts and practical application of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day-to-day operations of most laboratories in the chemical, biological, and medical sciences. This edition contains substantially updated chapters addressing the principles of most of the more common applications, such as: spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolved fluorescence and phosphorescence studies, circular dichroism, and pre-equilibrium spectroscopic techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Several chapters introduce subjects that have traditionally depended upon spectroscopy, such as basic enzyme kinetics, ligand binding, data handling, and the more recent interest in the study of protein and DNA stability. Finally, the concept of 'global analysis' is introduced to provide the reader with an insight into this method of utilizing the vast arrays of experimental data provided by current instrumentation.
Download or read book Spectrophotometry written by and published by Elsevier. This book was released on 2014-06-28 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is an essential handbook for anyone interested in performing the most accurate spectrophotometric or other optical property of materials measurements. The chapter authors were chosen from the leading experts in their respective fields and provide their wisdom and experience in measurements of reflectance, transmittance, absorptance, emittance, diffuse scattering, color, and fluorescence. The book provides the reader with the theoretical underpinning to the methods, the practical issues encountered in real measurements, and numerous examples of important applications. Written by the leading international experts from industry, government, and academia Written as a handbook, with in depth discussion of the topics Focus on making the most accurate and reproducible measurements Many practical applications and examples
Download or read book Accuracy in Spectrophotometry and Luminescence Measurements written by Radu Mavrodineanu and published by . This book was released on 1973 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.
Download or read book Current Catalog written by National Library of Medicine (U.S.) and published by . This book was released on with total page 1060 pages. Available in PDF, EPUB and Kindle. Book excerpt: First multi-year cumulation covers six years: 1965-70.
Download or read book National Library of Medicine Current Catalog written by National Library of Medicine (U.S.) and published by . This book was released on 1988 with total page 1068 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book NBS Technical Note written by and published by . This book was released on 1968 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analytical Toxicology for Poisoning Management and Toxicovigilance written by S. K. Gupta and published by Alpha Science Int'l Ltd.. This book was released on 2002 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers services of the Toxicology Laboratory from common general emergency medicine to specialized treatment units, defining procedures for critical quantitative analysis in treating acute poisoning cases. If the diagnosis or the prognosis is in doubt, active elimination therapy is considered or the administration of certain antidotes is contemplated. Hence, the book describes not only simple analytical techniques such as various advanced techniques such as High performance liquid chromatography Gas chromatography Ultraviolet-visible spectrophotometry and Atomic absorption spectrometry Presently detailed account of Instrumentation Operating procedures Possible interfaces in the methodology and Prototype examples of analytical procedures specifying the sensitivity of each test.
Download or read book Advances in Standards and Methodology in Spectrophotometry written by C. Burgess and published by Elsevier. This book was released on 2012-12-02 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: These conference proceedings form a worthy contribution to the literature on spectrometry reviving the tradition begun at the U.S. National Bureau of Standards conferences in the 1970's of holding interdisciplinary conferences on advances in the standardisation and methodology of spectrometry. The papers and poster abstracts presented in this volume maintain the high professional standards of these earlier NBS conferences, providing a basis for further progress in this discipline.
Download or read book Advances in Standards and Methodology in Spectrophotometry written by Ultraviolet Spectrometry Group (Great Britain) and published by Elsevier Publishing Company. This book was released on 1987 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: These conference proceedings form a worthy contribution to the literature on spectrometry reviving the tradition begun at the U.S. National Bureau of Standards conferences in the 1970's of holding interdisciplinary conferences on advances in the standardisation and methodology of spectrometry. The papers and poster abstracts presented in this volume maintain the high professional standards of these earlier NBS conferences, providing a basis for further progress in this discipline.
Download or read book Derivative Spectrophotometry and PAM Fluorescence in Comparative Biochemistry written by Vladimir S. Saakov and published by Springer. This book was released on 2015-12-09 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents various examples of how advanced fluorescence and spectroscopic analytical methods can be used in combination with computer data processing to address different biochemical questions. The main focus is on evolutionary biochemistry and the description of biochemical and metabolic issues; specifically, the use of pulse amplitude modulated fluorescence (PAM) for the functional analysis of the cellular state, as well as results obtained by means of the derivative spectroscopy method characterizing structural reorganization of a cell under the influence of external factors, are discussed. The topics presented here will be of interest to biologists, geneticists, biophysicists and biochemists, as well as experts in analytical chemistry, pharmaceutical chemistry and radio chemistry and radio activation studies with protonen and alpha-particles. It also offers a valuable resource for advanced undergraduate and graduate students in biological, physical and chemical disciplines whose work involves derivative spectrophotometry and PAM-fluorescence.
Download or read book Standards in Flourescence Spectrometry written by J. Miller and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 127 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Photoelectric Spectrometry Group was formed in July 1948 in Cambridge. The Group was born out of a need for a forum of users to discuss problems and methodology associated with the new era of photoelectric spectrometers. Over the years the aims and objectives of the Group have been broadened to include many aspects of ultraviolet and visible spectrometry. In 1973, the Group renamed itself the UV Spectrometry Group (UVSG). The techniques of fluorescence, diffuse reflectance, ORD and CD were included in the Group's interest. In 1979, the UVSG became a registered charity. The present Group membership is some 200 practising spectroscopists, mostly from the UK with a small but growing overseas membership. In August 1977, the UVSG Committee set up three Working Parties: Cells for UV-Visible Spectrophotometers; Photometric and Wavelength Standards; and the Calibration of Fluorimeters. It was felt that a wealth of information and expertise in the practice of spectrometry was available within the Group and that it was appropriate for this to be gathered together in the form of a number of monographs. Initially the intention was that these should be circulated only amongst the Group membership. However, the suggestion was made that these monographs would be of interest to other scientists outside our specialist Group. The conclusions of the first two Working Parties were combined in Volume 1 of this series, and this monograph summarizes the work of the third Working Party.
Download or read book Principles and Applications of Fluorescence Spectroscopy written by Jihad Rene Albani and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence spectroscopy is an important investigational tool in many areas of analytical science, due to its extremely high sensitivity and selectivity. With many uses across a broad range of chemical, biochemical and medical research, it has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems with extremely high resolution. It is particularly heavily used in the pharmaceutical industry where it has almost completely replaced radiochemical labelling. Principles and Applications of Fluorescence Spectroscopy gives the student and new user the essential information to help them to understand and use the technique confidently in their research. By integrating the treatment of absorption and fluorescence, the student is shown how fluorescence phenomena arise and how these can be used to probe a range of analytical problems. A key element of the book is the inclusion of practical laboratory experiments that illustrate the fundamental points and applications of the technique.
Download or read book Biomolecular and Bioanalytical Techniques written by Vasudevan Ramesh and published by John Wiley & Sons. This book was released on 2019-06-10 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.
