Download or read book Spatial and Temporal Analysis of Chitinase Accumulation and Pathogen Colonization in Soybeans with Tolerance to Phytophthora Sojae Infection written by Kitrina Murie Carlson and published by . This book was released on 2003 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tolerance to Phytophthora root rot (PRR) in soybean is a highly-heritable, multi-genic trait. Tolerance is a desirable trait in soybean lines because it is not race-specific and cannot be overcome by a buildup of a specific Phytophthora sojae races, unlike race-specific, single gene resistance. The plant-pathogen interactions occurring in the tolerant reaction to disease have not been thoroughly characterized. The objective of this research was to characterize components of the plant-pathogen interaction occurring in tolerant soybeans infected with P. sojae that may be involved in limiting pathogen colonization and disease development. One important aspect of this interaction is the timing of localized and systemic defense responses. Using chitinase as a marker of defense response activation, I characterized spatial and temporal patterns of chitinase isoform expression. I found that unique chitinase isoforms accumulate in soybean roots infected with P. sojae . Some of these isoforms are expressed earlier in tolerant cultivars than in susceptible cultivars and some of these isoforms are expressed systemically in tolerant cultivars and only locally in susceptible cultivars. This has helped to elucidate differences in the plant defense response between tolerant and susceptible cultivars. Another important component of this interaction is the events involved in pathogen colonization of the root. To analyze the timing and extent of P. sojae colonization of the soybean root system, I utilized real-time PCR to detect and quantify P. sojae within the soybean root system. My results suggest P. sojae colonizes tolerant cultivars more slowly and to a lesser degree than susceptible cultivars.

Download or read book Study of Incomplete Resistance to Phytophthora Sojae in Soybean written by Santiago Xavier Mideros Mora and published by . This book was released on 2006 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Phytophthora root and stem rot caused by Phytophthora sojae, is a serious limitation to soybean production in the United States. Extensive deployment of Rps genes in soybean cultivars has led to adaptations in the P. sojae populations. Partial resistance to P. sojae in soybeans is effective against all races of the pathogen and is a form of incomplete resistance where the plant reduces the rate of colonization of the pathogen. In addition to partial resistance other types of incomplete resistance have also been described. Rps2 is a single dominant gene that confers incomplete resistance in soybean hypocotyls. Root resistance, thought to be quantitatively inherited, is another form of race specific resistance that appears to function only in the roots. In order to differentiate partial resistance from the other types of incomplete resistance that are race specific, components of resistance were analyzed and the cytology of infection compared. For this study attempts to genetically transform P. sojae to express a marker gene were unsuccessful. Three components were measured (lesion length, oospore production and infection frequency) in 8 soybean genotypes that were inoculated with non-transformed P. sojae isolates on the roots. Light and epifluorescent microscopy were used to study transversal cuts of Trypan blue stained root samples that were also inoculated with P. sojae. Soybean partial resistance was found to be composed of various components that interact to produce the partial resistance phenotype for defense against P. sojae in the roots. It was also found that the Rps2 and root resistant genotypes had significantly reduced levels for all of the components of resistance studied in comparison to the partially resistant genotype Conrad. However, the high levels of partial resistance in Jack were indistinguishable from the Rps2 reaction for all the components studied. In the cytology study it was found that P. sojae penetrates into all the soybean incomplete resistant genotypes: partial resistant, Rps2, and root resistant. Several mechanisms of resistance were observed: i) the resistance phenotype (Rpsla) contained the pathogen biotrophic growth from between 0 to 24 hal in a hypersensitive response; ii) Rps2 and root resistance phenotypes, also stopped growth of the pathogen but this occurred between 24 and 48 hal in a delayed hypersensitive response and iii) the partial resistance and the susceptible phenotypes allowed biotrophic colonization ofF. sojae. Finally a methodology to differentiate among mechanisms of incomplete resistance to P. sojae in soybean was identified based on the number of dead cells and extent of colonization that differed in partial resistance and Rps2 phenotypes.

Download or read book Dissertation Abstracts International written by and published by . This book was released on 2003 with total page 730 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book American Doctoral Dissertations written by and published by . This book was released on 2002 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Plant Pattern Recognition Receptors written by Libo Shan and published by . This book was released on 2017 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Pattern Recognition Receptors: Methods and Protocolsaims to ensure successful results in the further study of this vital field." -- OCLC.

Download or read book Chemical Abstracts written by and published by . This book was released on with total page 2762 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Plant microbe Interactions 2 written by Gary Stacey and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant-Microbe Interactions, Volume 2 Volume 1 of this series has made its appearance and dealt forcefully with impor tant current topics in the field of plant-microbe interactions. We believe that the quality of those chapters was high and should serve as a focal point for the state of the art as well as an enduring reference. Volume 2 builds upon these accom plishments. Chapter 1 discusses the fascinating lipo-chitin signal molecules from Rhizo bium, aspects regarding their biosynthesis, and the basis for host specificity. These molecules are a cardinal example of how microorganisms influence plant development and stimulate speculation that they have identified a previously un known aspect of plant hormone activity. Chapter 2 continues the discussion of Rhizobium by considering the trafficking of carbon and nitrogen in nodules. Al though the ostensible advantage of nodules to plants is the fixation of atmos pheric nitrogen, the actual process involved in supplying reduced nitrogen to the plant host is complex.

Download or read book Plant Microbiomes for Sustainable Agriculture written by Ajar Nath Yadav and published by Springer Nature. This book was released on 2020-03-06 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book encompasses the current knowledge of plant microbiomes and their potential biotechnological application for plant growth, crop yield and soil health for sustainable agriculture. The plant microbiomes (rhizospheric, endophytic and epiphytic) play an important role in plant growth, development, and soil health. Plant and rhizospheric soil are a valuable natural resource harbouring hotspots of microbes, and it plays critical roles in the maintenance of global nutrient balance and ecosystem function. The diverse group of microbes is key components of soil–plant systems, where they are engaged in an intense network of interactions in the rhizosphere/endophytic/phyllospheric. The rhizospheric microbial diversity present in rhizospheric zones has a sufficient amount of nutrients release by plant root systems in form of root exudates for growth, development and activities of microbes. The endophytic microbes are referred to those microorganisms, which colonize in the interior of the plant parts, viz root, stem or seeds without causing any harmful effect on host plant. Endophytic microbes enter in host plants mainly through wounds, naturally occurring as a result of plant growth, or through root hairs and at epidermal conjunctions. Endophytes may be transmitted either vertically (directly from parent to offspring) or horizontally (among individuals). The phyllosphere is a common niche for synergism between microbes and plant. The leaf surface has been termed as phyllosphere and zone of leaves inhabited by microorganisms as phyllosphere. The plant part, especially leaves, is exposed to dust and air currents resulting in the establishments of typical flora on their surface aided by the cuticles, waxes and appendages, which help in the anchorage of microorganisms. The phyllospheric microbes may survive or proliferate on leaves depending on extent of influences of material in leaf diffuseness or exudates. The leaf diffuseness contains the principal nutrients factors (amino acids, glucose, fructose and sucrose), and such specialized habitats may provide niche for nitrogen fixation and secretions of substances capable of promoting the growth of plants. The microbes associated with plant as rhizospheric, endophytic and epiphytic with plant growth promoting (PGP) attributes have emerged as an important and promising tool for sustainable agriculture. PGP microbes promote plant growth directly or indirectly, either by releasing plant growth regulators; solubilization of phosphorus, potassium and zinc; biological nitrogen fixation or by producing siderophore, ammonia, HCN and other secondary metabolites which are antagonistic against pathogenic microbes. The PGP microbes belong to different phylum of archaea (Euryarchaeota); bacteria (Acidobacteria, Actinobacteria, Bacteroidetes, Deinococcus-Thermus, Firmicutes and Proteobacteria) and fungi (Ascomycota and Basidiomycota), which include different genera namely Achromobacter, Arthrobacter, Aspergillus, Azospirillum, Azotobacter, Bacillus, Beijerinckia, Burkholderia, Enterobacter, Erwinia, Flavobacterium, Gluconoacetobacter, Haloarcula, Herbaspirillum, Methylobacterium, Paenibacillus, Pantoea, Penicillium, Piriformospora, Planomonospora, Pseudomonas, Rhizobium, Serratia and Streptomyces. These PGP microbes could be used as biofertilizers/bioinoculants at place of chemical fertilizers for sustainable agriculture. The aim of “Plant Microbiomes for Sustainable Agriculture” is to provide the current developments in the understanding of microbial diversity associated with plant systems in the form of rhizospheric, endophytic and epiphytic. The book is useful to scientist, research and students related to microbiology, biotechnology, agriculture, molecular biology, environmental biology and related subjects.

Download or read book Bibliography of Agriculture written by and published by . This book was released on 1998 with total page 1960 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Plant Breeding for Biotic Stress Resistance written by Roberto Fritsche-Neto and published by Springer Science & Business Media. This book was released on 2012-10-01 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experience shows that biotic stresses occur with different levels of intensity in nearly all agricultural areas around the world. The occurrence of insects, weeds and diseases caused by fungi, bacteria or viruses may not be relevant in a specific year but they usually harm yield in most years. Global warming has shifted the paradigm of biotic stresses in most growing areas, especially in the tropical countries, sparking intense discussions in scientific forums. This book was written with the idea of collecting in a single publication the most recent advances and discoveries concerning breeding for biotic stresses, covering all major classes of biotic challenges to agriculture and food production. Accordingly, it presents the state-of-the-art in plant stresses caused by all microorganisms, weeds and insects and how to breed for them. Complementing Plant Breeding for Abiotic Stress Tolerance, this book was written for scientists and students interested in learning how to breed for biotic stress scenarios, allowing them to develop a greater understanding of the basic mechanisms of resistance to biotic stresses and develop resistant cultivars.

Download or read book Molecular Mechanism of Crucifer s Host Resistance written by Govind Singh Saharan and published by Springer Nature. This book was released on 2022-01-03 with total page 809 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book is a comprehensive compilation of applied knowledge for developing resistant varieties to all the major biotrophs, hemibiotrophs and necrotrophs pathogens of crucifers through the use of latest biotechnological approaches. The book includes, multi-component resistance, incorporation of non-host resistance gene, function of particular gene in resistance, expression of age related resistance, enhanced gene resistance, sources of alternative gene which enhance disease resistance, through the use of latest biotechnical approaches like proteomics, omics, transcriptomics and metabolomics. The book also explores the molecular basis of disease resistance, its biometabolomics activities in response to infection and interaction by the various biotrophs, hemibiotrophs and necrotrophs pathogens. The identification of R genes and its incorporation into agronomically superior varieties through use of molecular mechanisms is also explained. This compilation is immensely useful to the researchers especially Brassica breeders, teachers, extension specialists, students, industrialists, farmers, and all others who are interested to grow healthy, and profitable cruciferous crops all over the world.

Download or read book Fungal Pathology written by J.W. Kronstad and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book brings together twelve chapters on fungal pathogens with the goal of presenting an overview of the current areas of activity and the common themes that pervade research on these important organisms. The timing of the book is appropriate because we have gained sufficient insight from molecular genetic analyses to begin to make some comparisons between different fungal pathogens and to discuss the key advances that have been made. The chapters provide a broad survey of the important topics in fungal pathogenesis including morphogenesis, virulence, avirulence, and signaling. The reader also will fmd clear discussions of parasitism, mutualism, symbiosis, evolution, phylogeny and ecology for those fungi where these issues are especially important. Finally, many of the chapters in this book illustrate the fact that we are on the verge of a revolution in our understanding of fungal pathogens because of the application of genomics to these organisms and their hosts. The fungi included in this book represent many of the most intensively investigated fungal pathogens of plants; in this regard, a chapter is also included for pathogens in the Phytophthora group, even though these organisms are no longer classified as fungi. It is appropriate to include Phytophthora for historical reasons and, in addition, the insights in terms of pathogenesis and host-specific interactions are important to keep in mind when considering fungal pathogens. Chapters are also included on pathogens of insects and humans, as well as endophytic fungi.

Download or read book Biotechnological Approaches in Biocontrol of Plant Pathogens written by K.G. Mukerji and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological control offers a promising alternative to chemical control which can have adverse environmental implications. This volume contains 16 articles describing the most modern topics in biocontrol of plant pathogens, including risk analysis for the release of microbial antagonists, genetic engineering and application of tissue culture.

Download or read book SALICYLIC ACID written by Shamsul Hayat and published by Springer Science & Business Media. This book was released on 2013-04-16 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book “Salicylic acid: A Plant Hormone” was first published in 1997 and was praised for its excellent balance of traditional and modern topics. This time, we're building on the success of the prior edition to provide an even more effective second edition. The present book is comprised of 16 chapters highlighting the updated mechanisms of its biosynthesis, physiological role, its action in response to water deficit, relationship of SA with signal transduction, transport of SA and related compounds. Further, the interplay between environmental signals and SA, its impact on transport and distribution of sugars, salicylic acid mediated stress-induced flowering and some aspects of interplay of SA with JA during the establishment of plant resistance to pathogens with different types of nutrition and participation of peroxidases have also been discussed at length. Potential use of SA in food production and its efficiency on post-harvest of perishable crops as well as practical use of SA are also covered. ​ ​

Download or read book The Fungal Cell Wall written by Jean-Paul Latgé and published by Springer Nature. This book was released on 2020-08-12 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates, that the fungal cell wall is critical for the biology and ecology of all fungi and especially for human fungal pathogens. Readers will learn, that the composition of the fungal cell wall is a unique structure, which cannot be found in the human host. Consequently, the chapters outline, how the immune systems of both animals and humans have evolved to recognize conserved and unique elements of the fungal cell wall. As an application example, the authors also show, that the three-dimensional structures of the cell wall are excellent targets for the development of antifungal agents and chemotherapeutic strategies. With the combination of biological findings and medical outlooks, this volume is a fascinating read for scientists, clinicians and biomedical students.

Download or read book Comprehensive and Molecular Phytopathology written by Yuri Dyakov and published by Elsevier. This book was released on 2007-01-09 with total page 497 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers a collection of information on successive steps of molecular ‘dialogue’ between plants and pathogens. It additionally presents data that reflects intrinsic logic of plant-parasite interactions. New findings discussed include: host and non-host resistance, specific and nonspecific elicitors, elicitors and suppressors, and plant and animal immunity. This book enables the reader to understand how to promote or prevent disease development, and allows them to systematize their own ideas of plant-pathogen interactions. * Offers a more extensive scope of the problem as compared to other books in the market* Presents data to allow consideration of host-parasite relationships in dynamics and reveals interrelations between pathogenicity and resistance factors* Discusses beneficial plant-microbe interactions and practical aspects of molecular investigations of plant-parasite relationships* Compares historical study of common and specific features of plant immunity with animal immunity

Download or read book Electron Microscopy of Plant Pathogens written by Kurt Mendgen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.