Download or read book Solid State Nuclear Magnetic Resonance Studies of Prion Peptides and Proteins written by Jonathan Heller and published by . This book was released on 1997 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Solid State Nuclear Magnetic Resonance Studies of Prion Peptides and Proteins written by and published by . This book was released on 1997 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development of Techniques in Magnetic Resonance and Structural Studies of the Prion Protein written by Hans-Marcus Ludwig Bitter and published by . This book was released on 2000 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Peptide and Protein Supramolecular Assemblies Studied by Solid state NMR Spectroscopy written by Zhe Qi and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The non-crystalline, nature of supramolecular assemblies makes it difficult to investigate their structures by traditional methods such as X-ray crystallography. On the other hand, solid-state nuclear magnetic resonance (NMR) spectroscopy, a fast-developing technique that mainly detects the local atomic environment, is suitable for this purpose. In this dissertation, we start with a brief introduction in Chapter 1, then focus on the application of solid-state NMR spectroscopy on a few peptide and protein supramolecular assemblies that have biological significance: amyloid fibrils formed by deletion mutants of human prion protein (huPrP) 23-144 in Chapter 2, peptide amphiphile (PA) palmitoyl-VVAAEE-NH2 nanofibers in Chapter 3, and nanofibrils and aggregates of several peptide-chromophore-peptide triblock compounds in Chapter 4. One- and two-dimensional high-resolution magic angle spinning (MAS) solid-state NMR spectroscopy was employed to fast access the conformation and dynamics of these assemblies. Inter-atomic distances were further measured by dipolar recoupling pulse sequences. Other methods, such as transmission electron microscopy (TEM) and atomic force microscopy (AFM) to characterize the morphology of the supramolecular assemblies, X-ray diffraction to measure the interlayer distance, as well as thioflavin T fluorescence assay to monitor the kinetic of amyloid fibril formation, were also largely involved in the dissertation. We have found the shortest fragment of huPrP23-144 that can be expressed at reasonable yield in E. coli and retain the wild-type like amyloid folding. For the PA and peptide-chromophore-peptide triblock compounds we studied, parallel, in-register ß-sheet structure largely exist even for one compound that only forms small, random aggregates. Polymorphism was observed to exist extensively in both PA and the p-conjugated peptides. DRAWS to measure the homo-nuclear distance and TEDOR to measure the hetero-nuclear distance were proven to be two useful experiments to study the stacking in the self- and co-assemblies. Our results can facilitate the understanding of huPrP23-144 amyloid structure and the mechanism of relevant prion diseases, as well as promote more rational design of peptide-based materials that have novel supramolecular structures.

Download or read book Solid state Nuclear Magnetic Resonance Studies of the Major Coat Protein from the Filamentous Bacteriophage Fd written by Ana Carolina de Mattos Zeri and published by . This book was released on 2003 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Solid State Nuclear Magnetic Resonance Techniques for Determining Structure in Proteins and Peptides written by Peter Velling Bower and published by . This book was released on 2001 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book High resolution Structural Studies of Paramagnetic Proteins by Multidimensional Solid state Nuclear Magnetic Resonance Spectroscopy written by Philippe S. Nadaud and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Nuclear magnetic resonance (NMR) is one of the major spectroscopic techniques available for the characterization of molecular structure and conformational dynamics with atomic level detail. NMR relies on the intrinsic magnetic properties of certain nuclear isotopes, such as 1H, 13C, 15N, and 31P, which provide convenient, site-specific structural probes when placed inside a large external magnetic field. Recent developments in solid-state NMR (SSNMR) spectroscopy promise to enable detailed structural studies to be performed for important biological macromolecules, which are inherently insoluble but at the same time cannot be readily crystallized for analysis by X-ray diffraction. Prominent examples of systems of this type include large macromolecular complexes, membrane-bound peptides and proteins important in cell signaling, and fibrillar protein aggregates associated with the development of systemic and neurodegenerative human disorders, including Alzheimer's and Parkinson's diseases and type II diabetes.

Download or read book Solid state Nuclear Magnetic Resonance Studies of the Structure and Membrane Insertion of HIV Fusion Peptides written by Wei Qiang and published by . This book was released on 2009 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Solid State Nuclear Magnetic Resonance Studies of Structures and Membrane Locations of Peptides written by Li Xie and published by . This book was released on 2014 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein NMR for the Millennium written by N. Rama Krishna and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 345 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein NMR for the Millennium is the third volume in a special thematic series devoted to the latest developments in protein NMR under the Biological Magnetic Resonance umbrella. This book is divided into three major sections dealing with significant recent advances in the study of large proteins in solution and solid state, structure refinement, and screening of bioactive ligands. Key Features: TROSY, Segmental isotope labeling of proteins, Hydrogen bond scalar couplings, Structure refinement based on residual dipolar couplings, Written by the world's foremost experts who have provided broad leadership in advancing the protein NMR field.

Download or read book Solid State Nuclear Magnetic Resonance Methodology and Applications to Structure Determination of Peptides Proteins and Amyloid Fibrils written by Christopher P. Jaroniec and published by . This book was released on 2003 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: (Cont.) Complete sequence-specific 13C and 15N backbone and side- chain resonance assignments are obtained using two-dimensional 13C-13C and 15N-13C-3C chemical shift correlation experiments. Backbone torsion angles are measured directly using three-dimensional dipolar-chemical shift correlation experiments, which report on the relative orientations of 3C-15N, 3C-1H and 15N-'H dipolar tensors, and intramolecular 13C-15N distances in the 3-5 A range are determined using 3D TEDOR, resulting in about 60 constraints on the peptide structure. An atomic-resolution structure of the peptide consistent with the NMR constraints is calculated using simulated annealing molecular dynamics, and the results indicate that the peptide adopts an extended [beta]-strand conformation in the fibril.

Download or read book Protein NMR Techniques written by Alexander Shekhtman and published by . This book was released on 2012-01-01 with total page 518 pages. Available in PDF, EPUB and Kindle. Book excerpt: In its expanded third edition, this Methods in Molecular Biology volume offers techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing, materials lists, step-by-step protocols, troubleshooting tips and more.

Download or read book Structure Determination with Solid state NMR written by Ansgar Bernhard Siemer and published by . This book was released on 2006 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Experimental Approaches of NMR Spectroscopy written by The Nuclear Magnetic Resonance Society of Japan and published by Springer. This book was released on 2017-11-23 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the advanced developments in methodology and applications of NMR spectroscopy to life science and materials science. Experts who are leaders in the development of new methods and applications of life and material sciences have contributed an exciting range of topics that cover recent advances in structural determination of biological and material molecules, dynamic aspects of biological and material molecules, and development of novel NMR techniques, including resolution and sensitivity enhancement. First, this book particularly emphasizes the experimental details for new researchers to use NMR spectroscopy and pick up the potentials of NMR spectroscopy. Second, the book is designed for those who are involved in either developing the technique or expanding the NMR application fields by applying them to specific samples. Third, the Nuclear Magnetic Resonance Society of Japan has organized this book not only for NMR members of Japan but also for readers worldwide who are interested in using NMR spectroscopy extensively.

Download or read book High Resolution Nuclear Magnetic Resonance and Modeling Studies on Proteins written by Weon Tae Lee and published by . This book was released on 1992 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Solid state Nuclear Magnetic Resonance Structural Studies of the HIV 1 Fusion Peptide in the Membrane Environment written by Jun Yang and published by . This book was released on 2003 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Conformation of Y145Stop Prion Protein in Solution and Amyloid Fibrils Probed by Nuclear Magnetic Resonance Spectroscopy written by Yongjie Xia and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In Chapter 4, we investigate the intrinsic copper(II) binding site in huPrP23-144 by EPR spectroscopy and coupled PRE effects with solid state NMR spectroscopy to obtain the long range contacts between the flexible N-terminal tail and the rigid core region of huPrP23-144 in the fibrillar state. These results can be used as distance restraints to construct the conformational ensembles of the N-terminal domain. Interestingly, similar contacts were observed in huPrP23-144 in both physiological and fibrillar states. Finally, in Chapter 5, we present the studies of the residue-specific solvent accessibility in the core region of huPrP23-144 amyloid fibrils via PRE and hydrogen/deuterium exchange coupled with both solution and solid-state NMR spectroscopy. By comparing results from different experiments, a protection pattern in huPrP23-144 amyloid fibrils was found, in which residues 115 through 127 are buried inside a folded region.