Download or read book Solid state NMR Studies of Phospholipid Model Membranes and Membrane associated Macromolecules written by Jun-Xia Lu and published by . This book was released on 2007 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: The effect of cholesterol on the bicelles was further studied utilizing both solid-state 2H NMR and the EPR spin labeling approach. The results indicate a higher molecular ordering of the lipids and a higher alignment transition temperature of the bicelles in the presence of cholesterol.
Download or read book Solid state NMR Spectroscopic Studies of Proteins and Small Molecules in Phospholipid Membranes written by Shidong Chu and published by . This book was released on 2009 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: 2H, 31P, and 1H-MAS solid-state NMR spectroscopic techniques were used to elucidate the interaction between sorbate, a widely used antibacterial agent, and the lipid bilayers. In the membrane association process, sorbate are most likely interacting with the headgroups, rather than inserting deeply into the acyl chains region. Phospholamban (PLB) regulates calcium transport across the membranes via an inhibitory interaction with SERCA, the cardiac isoform of Ca2+-ATPase. Phosphorylation of PLB relieves the inhibition in a mechanism that is not completely understood. 15N chemical shift changes of ~20 ppm upon phosphorylation were observed in site-specific 15N-labeled PLB and phosphorylated PLB embedded in oriented lipid bilayers, indicating that phosphorylation of PLB alters the structural properties of the cytoplasmic domain with respect to the lipid bilayers. 2H and 15N NMR spectra of wild-type PLB and a N27A PLB mutant reconstituted into lipid bilayers indicate that the N27A mutation does not significantly change the side-chain or backbone dynamics of the transmembrane and cytoplasmic domains. However, dynamic changes are observed for the hinge region, in which greater mobility is observed for the CD3-labeled Ala24 N27A-PLB. This may help to understand why the N27A mutation in the hinge region of PLB leads to heart failures. A new approach for determining the membrane immersion depth of a spin-labeled probe was developed using paramagnetic relaxation enhancement (PRE) in solid-sate NMR spectroscopy. A DOXYL spin label was placed at different depths in the phospholipid bilayers and the resulting enhancements of the 31P spin-lattice relaxation (T1) times were measured and used to calculate the immersion depth. The 31P T1 values decreases steadily as the spin label moves closer to the surface and as the concentration of the spin-labeled lipids increases. These trends of enhanced relaxation vs. the position and the concentration of spin-labels indicate that PRE induced by the DOXYL spin label are significant to determine long distances over the whole depth range of the membranes. This approach may be a powerful biophysical method for measuring membrane protein immersion depth.
Download or read book Biophysical Studies of Lipid Membranes by Solid State NMR and Molecular Dynamics Simulations written by Bercem Dutagaci and published by . This book was released on 2013 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structural Studies of Membrane associated Complexes by Solid state NMR written by and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Solid state NMR Spectroscopic Studies on Phospholamban and Saposin C Proteins in Phospholipid Membranes written by Shadi Abu-Baker and published by . This book was released on 2007 with total page 195 pages. Available in PDF, EPUB and Kindle. Book excerpt: Solid-state NMR spectroscopic techniques were used to investigate two significant membrane proteins, phospholamban (PLB) and Saposin C (Sap C). For the first protein, analysis of the 2H and 31P solid-state NMR data of chemically synthesized WT-PLB and its phophorylated form (P-PLB) in 1-palmitoyl-2-oleoyl-sn-glycero-phosphocholine (POPC) multilamellar vesicles (MLVs) indicates that the interaction of P-PLB with POPC bilayers was less significant when compared to PLB. Moreover, the secondary structure using 13C=O site-specific isotopically labeled Ala15-PLB and Ala15-P-PLB in POPC bilayers suggests that this residue, located in the cytoplasmic domain, is a part of an alpha-helical structure for both PLB and P-PLB. Also, 2H NMR spectra of site-specific CD3-labeled WT-PLB and P-PLB at Ala15 exhibit one strong isotropic spectral component indicating the presence of additional motions as well as faster side-chain reorientations when compared with Leu51 and Ala24 representing the transmembrane domain. Conversely, the 15N Ala11 NMR spectrum of WT-PLB located on the cytoplasmic domain yields two dynamic components (powder pattern component and isotropic component) implying that the backbone dynamics of this residue exists in two populations: one that is immobile, and another which is motionally averaged on the NMR timescale. Upon phosphorylation, the 15N mobile component contribution increases. The POPC 15N NMR spectra indicate that the transmembrane domain has a tilt angle of 13 +/- 6° with respect to the mechanically oriented POPC bilayer normal and that the cytoplasmic domain of WT-PLB lies on the surface of the phospholipid bilayers. For the second protein, 2H and 31P solid-state NMR data of Sap C in dioleoylphosphatidylglycerol (DOPG) and dioleoylphosphatidylserine (DOPS) mixed bilayers indicates that Sap C is not inserting deep into the bilayers and that it has no preference to DOPS over DOPG. Finally, several other solid-state NMR spectroscopic experiments indicate that protonated Sap C disturbs 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-L-serine] (POPS) lipid bilayers and not the neutral POPC lipids.
Download or read book Development of New Methods for the Alignment of Longer Chain Phospholipids in Bicelles and Solid state NMR Studies of Phospholamban written by Elvis K. Tiburu and published by . This book was released on 2004 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: Magnetically aligned phospholipid bilayers or bicelles are model systems that mimic biological membranes for magnetic resonance studies. A long chain phospholipid bilayer system that spontaneously aligns in a static magnetic field was characterized utilizing solid-state NMR spectroscopy. The oriented membrane system was composed of a mixture of the bilayer-forming phospholipid palmitoylstearoylphosphatidylcholine (PSPC) and the short-chain phospholipid dihexanoylphosphatidylcholine (DHPC) that breaks up the extended bilayers into bilayered micelles or bicelles that are highly hydrated. Traditionally, the shorter 14-carbon chain phospholipid dimyristoyl-phosphatidylcholine (DMPC) has been utilized as the bilayer-forming phospholipid in bicelle studies. The effect of cholesterol in bicelles containing chain perdeuterated DMPC, a partially deuterated (a-[2,2,3,4,4,6-2H6]) cholesterol, and stearic acid-d35 has been reported as a function of temperature using 2H solid-state NMR spectroscopy. The order parameters of the labeled probes were calculated and compared with values obtained from unoriented samples in the literature. In addition, 2H solid-state NMR spectroscopy was used to investigate the orientation and side chain dynamics of specific-labeled methyl groups of leucines in PLB in unoriented as well as in magnetically and mechanically aligned phospholipids bilayers. Phospholamban (PLB), is a 52 amino-acid protein that assembles into a pentamer in cardiac sarcoplasmic reticulum membranes. The protein is a key regulator of the calcium ATPase through an inhibitory association that can be reversed by phosphorylation. From the 2H NMR studies, the exhibited line shapes were characteristic of either methyl group reorientation about the C-C bond axis, or by additional wobbling motion about the Ca-Cb and Cb-Cg bond axes. Using 15N NMR spectroscopy, the backbone dynamics of PLB as well as the orientation of PLB in the model membranes were also studied. A comparative study of PLB in shorter chain DMPC as well as in longer chain DOPC bilayers was conducted to determine the effect of hydrophobic length on peptide orientation. Taken together, the results are discussed in terms of the structure of PLB in phospholipid bilayers previously proposed on the basis of mutational and molecula.
Download or read book Membrane Protein Structure Determination written by Jean-Jacques Lacapère and published by Methods in Molecular Biology. This book was released on 2010-08-06 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology.
Download or read book Peptide Lipid Interactions written by Sidney A. Simon and published by Academic Press. This book was released on 2002-11-13 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction
Download or read book Study of Bacteriorhodopsin in a Controlled Lipid Environment written by Vivien Yeh and published by Springer. This book was released on 2018-10-26 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the study of how the properties of nanodiscs, such as lipid composition and size, influence the function of the embedding integral membrane protein, bacteriorhodopsin. The author performed systematic studies to show that the lipid composition and the charge of the hydrophobic head and the structure of hydrophilic tails affect the photocycle pathway of bacteriorhodopsin, which is closely associated with its proton-pumping activity. Furthermore, the author demonstrated a highly efficient method for extracting membrane proteins directly from the biological membrane, preserving protein conformation, function and essential native lipids. This book demonstrates optimization and sample preparation, and presents practical methods of preparing membrane protein-embedded nanodisc samples for biophysical studies, which benefit structural and functional studies in the field of membrane protein characterization, both.
Download or read book Antimicrobial Peptides written by Katsumi Matsuzaki and published by Springer. This book was released on 2019-04-12 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents an overview of antimicrobial peptides (AMPs), their mechanisms of antimicrobial action, other activities, and various problems that must still be overcome regarding their clinical application. Divided into four major parts, the book begins with a general overview of AMPs (Part I), and subsequently discusses the various mechanisms of antimicrobial action and methods for researching them (Part 2). It then addresses a range of activities other than antimicrobial action, such as cell penetration, antisepsis, anticancer, and immunomodulatory activities (Part 3), and explores the prospects of clinical application from various standpoints such as the selective toxicity, design, and discovery of AMPs (Part 4). A huge number of AMPs have been discovered in plants, insects, and vertebrates including humans, and constitute host defense systems against invading pathogenic microorganisms. Consequently, many attempts have been made to utilize AMPs as antibiotics. AMPs could help to solve the urgent problem of drug-resistant bacteria, and are also promising with regard to sepsis and cancer therapy. Gathering a wealth of information, this book will be a bible for all those seeking to develop antibiotics, anti-sepsis, or anticancer agents based on AMPs.
Download or read book Phospholipids Handbook written by Gregor Cevc and published by CRC Press. This book was released on 1993-08-02 with total page 1162 pages. Available in PDF, EPUB and Kindle. Book excerpt: Employing a multidisciplinary approach to phospholipid research, this work catalogues the current knowledge of this class of molecules and details the general, chemical, physical and structural properties of phospholipid monolayers and bilayers. Phospholipid applications are also covered.
Download or read book Phosphorous 31 NMR written by David G. Gorenstein and published by Academic Press. This book was released on 2012-12-02 with total page 619 pages. Available in PDF, EPUB and Kindle. Book excerpt: Phosphorous-31 NMR: Principles and Applications focuses on the evolution of phosphorus-31 nuclear magnetic resonance (NMR) as a widely applied spectroscopic probe of the structure and dynamics of phosphorus-containing compounds. The selection first offers information on the principles and empirical observations of phosphorus-31 chemical shifts and the principles and applications of phosphorus-31 spin-spin coupling constants. Discussions focus on introduction and basic principles, directly bonded phosphorus coupling constants, and two-bond and three-bond coupling constants. The text then ponders on phosphorus-31 NMR of enzyme complexes and paramagnetic probes of enzyme complexes with phosphorus-containing compounds. The manuscript elaborates on the use of chiral thiophosphates and the stereochemistry of enzymatic phosphoryl transfer and DNA and RNA conformations. Topics include helix-coil transitions, sequence dependence of double-stranded DNA conformations, dynamic behavior of RNA and DNA, biological and genetic significance, and stereochemical problems studied with chiral thiophosphates. The conformation and dynamics of nucleic acids and phosphoproteins, relaxation behavior of nucleic acids, and theory and applications of solid-state phosphorus-31 NMR to nucleic acids are also discussed. The selection is a valuable reference for readers interested in the principles and applications of phosphorus-31 nuclear magnetic resonance.
Download or read book Multidimensional Solid State NMR and Polymers written by Klaus Schmidt-Rohr and published by Elsevier. This book was released on 2012-12-02 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: NMR spectroscopy is the most valuable and versatile analytical tool in chemistry. While excellent monographs exist on high-resolution NMR in liquids and solids, this is the first book to address multidimensional solid-state NMR. Multidimensional techniques enable researchers to obtain detailed information about the structure, dynamics, orientation, and phase separation of solids, which provides the basis of a better understanding of materials properties on the molecular level.Dramatic progress-much of it pioneered by the authors-has been achieved in this area, especially in synthetic polymers. Solid-state NMR now favorably competes with well-established techniques, such as light, x-ray, or neutron scattering, electron microscopy, and dielectric and mechanical relaxation.The application of multidimensional solid-state NMR inevitably involves use of concepts from different fields of science. This book also provides the first comprehensive treatment of both the new experimental techniques and the theoretical concepts needed in more complex data analysis. The text addresses spectroscopists and polymer scientists by treating the subject on different levels; descriptive, technical, and mathematical approaches are used when appropriate. It presents an overview of new developments with numerous experimental examples and illustrations, which will appeal to readers interested in both the information content as well as the potential of solid-state NMR. The book also contains many previously unpublished details that will be appreciated by those who want to perform the experiments. The techniques described are applicable not only to the study of synthetic polymers but to numerous problems in solid-state physics, chemistry, materials science, and biophysics. Presents original theories and new perspectives on scattering techniques Provides a systematic treatment of the whole subject Gives readers access to previously unpublished material Includes extensive illustrations
Download or read book Experimental Approaches of NMR Spectroscopy written by The Nuclear Magnetic Resonance Society of Japan and published by Springer. This book was released on 2017-11-23 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the advanced developments in methodology and applications of NMR spectroscopy to life science and materials science. Experts who are leaders in the development of new methods and applications of life and material sciences have contributed an exciting range of topics that cover recent advances in structural determination of biological and material molecules, dynamic aspects of biological and material molecules, and development of novel NMR techniques, including resolution and sensitivity enhancement. First, this book particularly emphasizes the experimental details for new researchers to use NMR spectroscopy and pick up the potentials of NMR spectroscopy. Second, the book is designed for those who are involved in either developing the technique or expanding the NMR application fields by applying them to specific samples. Third, the Nuclear Magnetic Resonance Society of Japan has organized this book not only for NMR members of Japan but also for readers worldwide who are interested in using NMR spectroscopy extensively.
Download or read book Research Awards Index written by and published by . This book was released on 1987 with total page 826 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Membrane Organization and Dynamics written by Amitabha Chattopadhyay and published by Springer. This book was released on 2017-12-06 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume brings together information on membrane organization and dynamics from a variety of spectroscopic, microscopic and simulation approaches, spanning a broad range of time scales. The implication of such dynamic information on membrane function in health and disease is a topic of contemporary interest. The chapters cover various aspects of membrane lipid and protein dynamics, explored using a battery of experimental and theoretical approaches. The synthesis of information and knowledge gained by utilizing multiple approaches will provide the reader with a comprehensive understanding of the underlying membrane dynamics and function, which will help to develop robust dynamic models for the understanding of membrane function in healthy and diseased states. In the last few years, crystal structures of an impressive number of membrane proteins have been reported, thanks to tremendous advances in membrane protein crystallization techniques. Some of these recently solved structures belong to the G protein-coupled receptor (GPCR) family, which are particularly difficult to crystallize due to their intrinsic flexibility. Nonetheless, these static structures do not provide the necessary information to understand the function of membrane proteins in the complex membrane milieu. This volume will address the dynamic nature of membrane proteins within the membrane and will provide the reader with an up-to date overview of the theory and practical approaches that can be used. This volume will be invaluable to researchers working in a wide range of scientific areas, from biochemistry and molecular biology to biophysics and protein science. Students of these fields will also find this volume very useful. This book will also be of great use to those who are interested in the dynamic nature of biological processes.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
