Download or read book Solid state NMR Structural Studies of Proteins Using Cyclen Based Paramagnetic Metal Chelating Probes written by Rajith Madushanka Jayasinha Arachchige and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The capability of solid-state NMR spectroscopy to provide site-specific atomic-level information makes it one of the main tools used in the structural and dynamic analysis of complex non-crystalline biological macromolecules. However, the challenges associated with measurements of unambiguous long-range distance restraints between nuclear spins by conventional solid-state NMR methods have a significant impact on the ability to determine three-dimensional structures of proteins and nucleic acids. Recently, it has been shown that paramagnetism-based solid-state NMR methods can be utilized to overcome this scarcity of long-range structural information. Electron-nucleus through space dipolar interactions in proteins containing paramagnetic metal centers lead to NMR spectral effects including paramagnetic relaxation enhancements (PREs) and pseudocontact shifts (PCSs), which can provide structural restraints up to 30 A° and beyond exceeding by four to five-fold the length scales accessible by conventional methods. For natively diamagnetic proteins with no endogenous paramagnetic metal centers can be introduced by covalently linking to the protein small molecule tags containing high affinity metal binding motifs. The focus of this research was to develop new thiol-reactive mono and bidentate compact paramagnetic metal chelating probes based on the cyclen manifold for improved solid-state NMR PRE and PCS measurements in natively diamagnetic proteins. The single arm TETAC tag was able to overcome the key shortcomings associated with the more bulky and flexible EDTA type metal-binding tags used in initial paramagnetic solid-state NMR studies. Relative to PREs, the magnitude of which depends primarily on the electron-nucleus distance, the measurements of PCS effects are far more challenging due to an additional dependence on the location of the nucleus in the frame of the g-tensor of the unpaired electron spin of the paramagnetic center. Specifically, such measurements are susceptible to the flexibility of the paramagnetic tag, which can considerably reduce the PCS magnitude or average the effect altogether. To alleviate this problem compact cyclen based tags, which can rigidly be attached to the protein via two disulfide bridges, were designed. The new double arm tags, 2,2'-(1,4,7,10-tetraazacyclododecane-1,7-diyl)bis(N-(2-(pyridin-2-yldisulfanyl)ethyl)acetamide) and 2,2'-(4,10-bis(2-(pyridin-2-yldisulfanyl)ethyl)-1,4,7,10-tetraazacyclododecane-1,7-diyl)diacetic acid, are able to immobilize paramagnetic Co(II) ions on the model 56 amino acid residue protein, B1 immunoglobulin-binding domain of protein G, and successfully overcome the PCS averaging issues associated with the monodentate metal chelating probes. In addition, the same tags loaded with Cu(II) enable solid-state NMR PRE measurements to be performed to yield additional restraints on the protein structure.

Download or read book Solid State NMR Structural Studies of Proteins Modified with Paramagnetic Tags written by Ishita Sengupta and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Finally, in Chapter 5, we present solid-state NMR studies of the K28C mutant of protein GB1 intentionally modified with 2-[1,4,7,10-tetraazacyclododecan-1-yl]-ethanethiol (TETAC) side chains with a high affinity for transition metal ions like Cu2+, ZnZn2+, Co2+ etc.

Download or read book High resolution Structural Studies of Paramagnetic Proteins by Multidimensional Solid state Nuclear Magnetic Resonance Spectroscopy written by Philippe S. Nadaud and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Nuclear magnetic resonance (NMR) is one of the major spectroscopic techniques available for the characterization of molecular structure and conformational dynamics with atomic level detail. NMR relies on the intrinsic magnetic properties of certain nuclear isotopes, such as 1H, 13C, 15N, and 31P, which provide convenient, site-specific structural probes when placed inside a large external magnetic field. Recent developments in solid-state NMR (SSNMR) spectroscopy promise to enable detailed structural studies to be performed for important biological macromolecules, which are inherently insoluble but at the same time cannot be readily crystallized for analysis by X-ray diffraction. Prominent examples of systems of this type include large macromolecular complexes, membrane-bound peptides and proteins important in cell signaling, and fibrillar protein aggregates associated with the development of systemic and neurodegenerative human disorders, including Alzheimer's and Parkinson's diseases and type II diabetes.

Download or read book Protein NMR written by Lawrence Berliner and published by Springer. This book was released on 2015-08-24 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers new techniques in protein NMR, from basic principles to state-of-the-art research. It covers a spectrum of topics ranging from a “toolbox” for how sequence-specific resonance assignments can be obtained using a suite of 2D and 3D NMR experiments and tips on how overlap problems can be overcome. Further topics include the novel applications of Overhauser dynamic nuclear polarization methods (DNP), assessing protein structure, and aspects of solid-state NMR of macroscopically aligned membrane proteins. This book is an ideal resource for students and researchers in the fields of biochemistry, chemistry, and pharmacology and NMR physics. Comprehensive and intuitively structured, this book examines protein NMR and new novel applications that include the latest technological advances. This book also has the features of: • A selection of various applications and cutting-edge advances, such as novel applications of Overhauser dynamic nuclear polarization methods (DNP) and a suite of 2D and 3D NMR experiments and tips on how overlap problems can be overcome • A pedagogical approach to the methodology • Engaging the reader and student with a clear, yet critical presentation of the applications

Download or read book NMR of Proteins written by Clore and published by CRC Press. This book was released on 1993 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Determination of structures of larger proteins in solution by three- and four-dimensional heteronuclear magnetic resonance spectroscopy. Methodological advances in protein NMR. Determination of high-resolution NMR structures of proteins. Multidimensional NMR studies of immunosuppressant/immunophilin complexes. NMR studies of the structure and role of modules involved in protein-protein interactions. NMR structural studies of membrane proteins. Heteronuclear NMR studies of the molecular synamics of staphylococcal nuclease. Study of protein dynamics by NMR. The folding, stability and dynamics of T4 lysozyme: a perspective using nuclear magnetic resonance.

Download or read book Structural Studies by Solid State and Solution NMR Protein protein and Ligand protein Interactions written by Elina Lisitsin and published by . This book was released on 2011 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book High Resolution Structural Studies of Membrane Proteins Using Solid State NMR written by Lubica Aslimovska and published by . This book was released on 2007 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Dynamic and Structural Studies of Membrane Proteins by Oriented Sample Solid State NMR written by Emmanuel Oluwaseyi Awosanya and published by . This book was released on 2020 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Solid state NMR Studies of Protein Structures and Molecular Dynamics written by Valérie Copié and published by . This book was released on 1990 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biological NMR Spectroscopy written by John L. Markley and published by Oxford University Press. This book was released on 1997-01-30 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a critical assessment of progress on the use of nuclear magnetic resonance spectroscopy to determine the structure of proteins, including brief reviews of the history of the field along with coverage of current clinical and in vivo applications. The book, in honor of Oleg Jardetsky, one of the pioneers of the field, is edited by two of the most highly respected investigators using NMR, and features contributions by most of the leading workers in the field. It will be valued as a landmark publication that presents the state-of-the-art perspectives regarding one of today's most important technologies.

Download or read book Protein Structure Determination by Paramagnetic NMR and Computational Hybrid Approach written by Kala Bharath Pilla and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Computational modelling of proteins that rely on either de novo or evolutionary based approaches often produce poor quality structures, primarily due to the limitations in their algorithms or forcefields. Traditional experimental techniques such as X-ray crystallography depend on narrow set of crystallographic conditions while solution/solid state nuclear magnetic resonance (NMR) spectroscopy relies on cumbersome spectral analysis and complete resonance assignments. These traditional approaches are slow and costly endeavours. Computational/experimental hybrid approaches on the other hand provide a new avenue for reliable, rapid and cost-effective structure determination. Paramagnetic NMR offers easy generation of useful and sparse structural information which can be implemented as restraints in structure prediction algorithms. Pseudocontact shifts (PCS) are the most powerful of structural restraints generated by paramagnetic NMR which are long range in nature and can be easily obtained by simple 2D NMR experiments. This thesis demonstrates different approaches involved in protein structure calculations using PCS restraints in Rosetta. Chapter 2 demonstrates structure determination using PCS restraints exclusively obtained from protein samples in microcrystalline state by magic angle spinning (MAS) NMR spectroscopy. Chapter 3 discusses the implementation of using PCS data from multiple metal centres to precisely determine the location of spins in space in a manner analogues to GPS-satellites. Chapter 4 extends the usage of PCS data from multiple metal centres to capture distinct conformational states in proteins. Chapter 5 demonstrates new techniques especially developed for structure determination of large proteins involving super secondary structure motifs (Smotifs) and data driven iterative resampling. These different computational techniques serve the goal of determining accurate 3D models using minimal experimental data, which are applicable to proteins systems that are currently beyond the realm of traditional experimental approaches.

Download or read book Structural and Dynamical Studies of Metal Containing Proteins by NMR written by Milka Kostic and published by . This book was released on 2004 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structural Studies of Peptides and Proteins by NMR written by Katherine Justine Nielsen and published by . This book was released on 1995 with total page 704 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Experimental Approaches of NMR Spectroscopy written by The Nuclear Magnetic Resonance Society of Japan and published by Springer. This book was released on 2017-11-23 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the advanced developments in methodology and applications of NMR spectroscopy to life science and materials science. Experts who are leaders in the development of new methods and applications of life and material sciences have contributed an exciting range of topics that cover recent advances in structural determination of biological and material molecules, dynamic aspects of biological and material molecules, and development of novel NMR techniques, including resolution and sensitivity enhancement. First, this book particularly emphasizes the experimental details for new researchers to use NMR spectroscopy and pick up the potentials of NMR spectroscopy. Second, the book is designed for those who are involved in either developing the technique or expanding the NMR application fields by applying them to specific samples. Third, the Nuclear Magnetic Resonance Society of Japan has organized this book not only for NMR members of Japan but also for readers worldwide who are interested in using NMR spectroscopy extensively.

Download or read book NMR of Paramagnetic Molecules in Biological Systems written by Ivano Bertini and published by Benjamin-Cummings Publishing Company. This book was released on 1986 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biomolecular NMR Spectroscopy written by Andrew J. Dingley and published by IOS Press. This book was released on 2011 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear Magnetic Resonance (NMR) spectroscopy is the most powerful technique for characterization of biomolecular structures at atomic resolution in the solution state. This timely book, entitled "Biomolecular NMR Spectroscopy," focuses on the latest state-of-the-art NMR techniques for characterization of biological macromolecules in the solid and solution state. The editors, Dr. Andrew Dingley (University of Auckland, New Zealand) and Dr. Steven Pascal (Massey University, New Zealand) have organized the book into four sections, covering the following topics: sample preparation, structure and dynamics of proteins, structure and dynamics of nucleic acids and protein-nucleic acid complexes, and rapid and hybrid techniques--

Download or read book Modern NMR Methodology written by Henrike Heise and published by Springer. This book was released on 2014-07-08 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: NMR Spectroscopy for Chemical Analysis at Low Magnetic Fields, by Stefan Glöggler, Bernhard Blümich, Stephan Appelt Dynamic Nuclear Hyperpolarization in Liquids, by Ulrich L. Günther NMR with Multiple Receivers, by Eriks Kupce TROSY NMR Spectroscopy of Large Soluble Proteins, by Yingqi Xu, Stephen Matthews Solid-State NMR Spectroscopy of Proteins, by Henrik Müller, Manuel Etzkorn, Henrike Heise Paramagnetic Solid-State Magic-Angle Spinning NMR Spectroscopy, by Guido Pintacuda, Gwendal Kervern