Download or read book Smith River Watershed Bacteria Source Tracking Study written by CH2M HILL (Firm) and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microbial Source Tracking written by Jorge W. Santo Domingo and published by Emerging Issues in Food Safety. This book was released on 2007 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a state-of-the-art review of the current technology and applications being utilized to identify sources of fecal contamination in waterways. - Serves as a useful reference for researchers in the food industry, especially scientists investigating etiological agents responsible for food contamination. - Provides background information on MST methods and the assumptions and limitations associated with their use. - Covers a broad range of topics related to MST, including environmental monitoring, public health and national security, population biology, and microbial ecology. - Offers valuable insights into future research directions and technology developments.
Download or read book Bacterial Source Tracking in Impaired Watersheds written by Emily Catherine Martin and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacterial contamination due to excessive levels of bacteria is a confounding problem and remediation of impaired watersheds relies on the detection of fecal indicator bacteria and then assessing the source of said bacteria. Bacterial source tracking (BST) is an approach for assessing potential sources of this contamination. The purpose of this study was to utilize both cultivation-independent and -dependent methods to improve the ability to track sources of fecal contamination. First, E. coli community composition was assessed across three standard water quality assessments including USEPA Methods 1603 and 1604, and Colilert®, to determine their impact on BST library-based performance. Results indicate that the three assessed methods of enumeration and isolation may select for different populations of E. coli and standardized methods may be warranted if library-dependent BST is part of a research plan. Next, BST techniques were used to enumerate and characterize E. coli communities across various dairy manure management techniques used in the Leon River watershed in central Texas to determine effectiveness of BST efforts in tracking contamination from dairy manure. Results of this study indicated that manure and effluent management strategies which employed means to remove solids from the manure tended to decrease the levels of E. coli in the effluent. Some E. coli genotypes were found across the managerial treatments even though there were no clear seasonal trends or site groupings among the dataset. The vast majority of the isolates classified using the Texas E. coli BST library were correctly classified back to their major source class, thus increasing confidence in the methods currently being utilized to track dairy fecal contributions in this Central Texas watershed. Finally, deer bacterial fecal communities from south and central Texas were analyzed using 454-pyrosequencing to assess the potential for the development of a deer-specific BST marker. Microbial communities did not cluster by site or year suggesting that deer fecal communities in these Texas regions are stable over time and could be amenable to marker development. The electronic version of this dissertation is accessible from http://hdl.handle.net/1969.1/149494
Download or read book Microbial Source Tracking Methods Applications and Case Studies written by Charles Hagedorn and published by Springer Science & Business Media. This book was released on 2011-06-08 with total page 645 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding the origin of fecal pollution is essential in assessing potential health risks as well as for determining the actions necessary to remediate the quality of waters contaminated by fecal matter. As a result, microbial source tracking (MST) has emerged as a field that has evolved and diversified rapidly since the first approaches were described only a decade ago. In response to the emergence of MST, there have been three large multi-laboratory method comparison studies (two in the US and one in Europe), plus numerous workshops, book chapters, and review articles dedicated to synthesizing information on the topic. Furthermore, a federal (USEPA) guide document describing the uses and limitations of MST methods was published in 2005, and a book dedicated to MST as an emerging issue in food safety was published in 2007. These documents provide a collective body of literature on MST that is both conflicting and complementary, often repetitious, and difficult to condense and interpret. In addition, it does not reflect the current diversity of MST approaches with different organisms, newer methodologies such as quantitative PCR, and anthropogenic chemicals, nor does it embrace the scope of MST research being conducted around the world. The three editors of the book, all with extensive MST expertise, have developed chapters and invited authors who reflect the rich diversity and truly international scope of MST. The unifying theme throughout the book is the design of more standardized approaches to MST that include performance criteria (regardless of method or organism), plus recommendations for field study design and MST implementation. The editors intend that this book will serve as a valuable reference for all those who are involved with
Download or read book Bacterial Source Tracking in the Sinking Creek Watershed Using Antibiotic Resistance Analysis and Ribotyping written by and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Fecal pollution of surface water is a significant environmental health issue. Indicator organisms are used to monitor microbial water quality, but often their presence does not coincide with the presence of pathogens. Bacterial source tracking is a term describing methods to determine the origin of fecal pollution based on bacterial traits. The objective of this research is to evaluate the use of 2 bacterial source tracking techniques, antibiotic resistance analysis (ARA) and ribotyping, to determine the sources of bacteria isolated from Sinking Creek. Based on the results of this study, ARA and ribotyping are not useful techniques for identifying sources of fecal pollution in Sinking Creek. ARA classification rates were low, and ribotype pattern generation success was 37%. The results of this study bring into question the reliability and reproducibility of these 2 source tracking methods for routine use in small watersheds.
Download or read book Microbial Source Tracking written by Michèle Gourmelon and published by Frontiers Media SA. This book was released on 2022-01-18 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Report on the Bacteria Source Tracking Project written by Deborah D. Ross and published by . This book was released on 2004 with total page 24 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Utility of Microbial Source tracking Markers for Assessing Fecal Contamination in the Portage River Watershed Northwestern Ohio 2008 written by Christopher M. Kephart and published by . This book was released on 2010 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Palmer River Microbial Source Tracking Study written by Environmental Science Services, Inc and published by . This book was released on 2003 with total page 18 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microbial Source Tracking written by Natsuko N. Merrick and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The molecular based, quantitative PCR assay was compared with the traditional viable E. coli count assay and a significant (P
Download or read book Understanding Contributions of Fecal Bacteria Sources in Wyoming Rivers to Inform Pollution Management written by Kelsey C. Ruehling and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pathogen contamination is the leading cause of impairment in American rivers. Most water quality monitoring programs use fecal indicator bacteria (FIB) like Escherichia coli, as a proxy for fecal pollution and dangerous enteric pathogens. However, standard FIB enumeration methods are unable to differentiate pollution sources, limiting resource managers ability to implement targeted management actions. Uncertainty about source contributions can be overcome with new molecular and genetic techniques like microbial source tracking (MST). We employed community-based microbial source tracking using high-throughput sequencing (HTS, 16S amplicon sequencing) of aquatic and fecal samples to understand fecal source contributions to surface water microbial communities in two watersheds in Wyoming, USA. While watershed-differences led to some variation of fecal pollution and aquatic microbial communities between the two watersheds, common patterns and drivers emerged. Both watersheds had higher bacterial diversity, and distinct bacterial assemblages in headwater streams while further downstream locations with legacy anthropogenic alterations had less diverse and more similar bacterial communities. We found that higher microbial diversity and increased fecal bacterial loading were related to changes in stream hydrology, and were most pronounced during periods of high runoff from snowmelt. We found no relationship between fecal bacterial sources from MST and standard E. coli enumeration, indicating E. coli may not be a good proxy for fecal pollution in these systems. This research will inform mitigation plans that resource managers are currently developing by providing novel insights about fecal bacterial sources for Wyoming streams that were recently listed as impaired.
Download or read book Microbial Pollution Source Identification in Rural written by Amira Oun H. Oun and published by . This book was released on 2016 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microbial Source Tracking in the South Dry Sac Watershed written by Emily L. Sturnfield and published by . This book was released on 2011 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: Karst watersheds are an important resource that can be easily contaminated. The South Dry Sac Watershed near Springfield, MO presents a unique opportunity to study the combined effects of urban and rural runoff on a karst river system, as the system changes from rural to urban within the span of a few kilometers. Previous studies have indicated that the watershed is impaired by fecal bacteria, but sources of the bacteria were not investigated. This study traced sources of fecal coliform bacteria (bovine, human) in the watershed and related it to land use. Bovine source Bacteroides was prevalent throughout the field area, while human source Bacteroides was present in lower levels. Results indicate higher human fecal loading at sites located near areas using septic waste treatment. Additional water quality parameters helped distinguish water signatures of the urban from the rural areas within the watershed. The two most contaminated sites were tracked to both bovine and human sources, were downstream from known larger-scale cattle operations, and received drainage from unsewered portions of the study area. The range of values obtained and their variation with time provides a good framework for watershed management.
Download or read book Scientific Investigations Report written by Sharon E. Kroening and published by . This book was released on 2004 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Water resources Investigations Report written by and published by . This book was released on 2003 with total page 1342 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Smith River Bacteriological Study written by California. Bureau of Sanitary Engineering and published by . This book was released on 1969 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Host Specific Microbial Source Tracking and Quantification Using a Partial 16SrRNA Sequence of Bacteroides on the Finley River a Tributary of the James River in Southwest Missouri written by Neil J. Van Asch and published by . This book was released on 2009 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial source tracking (MST) is a general term given to any process which aims to determine the source of microbial contamination found in an environmental (usually water) sample. These methods include both phenotypic and genotypic strategies. In this study, quantitative PCR was used to evaluate water samples for species specific (cow and human) Bacteroides in the Finley River. The Finley River is susceptible to impacts from both bovine and humans as it flows through farmland and urban areas. Water samples were collected from sites identified in a previous study that had high E. coli levels. Two samples were collected at each site (One sample for enumeration of E. coli and the other to assay for Bacteroides). Sampling was done approximately three times per month for one year. For each sampling event, pH, DO, turbidity, water temperature, flow rate, and rainfall were recorded. The results showed lower than expected cow markers throughout the sampling period; while human markers were much more prevalent than cow markers during the drier (and warmer) months. A correlation was found between human specific Bacteroides markers and E. coli at one site, but not at either of the other two sites. This method affords more informative surveillance data to area health departments so more appropriate remediation strategies can be developed.
