Download or read book SMC Complexes written by Anjana Badrinarayanan and published by . This book was released on 2019 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic Structural Maintenance of Chromosome (SMC) proteins. The protocols explore numerous subjects such as depletion systems to assess SMC function, genetic manipulation of SMC proteins, chromosomal, biochemical, and microscopy-based assays of SMC activity, as well as theoretical modeling of SMC activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SMC Complexes: Methods and Protocols will greatly aid researchers looking into the mechanism of action of SMC complexes on DNA. Chapters 3, 10 and 19 are available Open Access under a CC BY 4.0 license via link.springer.com.
Download or read book Chromosome Architecture written by Mark C. Leake and published by Humana. This book was released on 2016-06-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details a valuable collection of protocols and reviews, such as emerging experimental and theoretical approaches. These approaches have resulted in a substantial improvement in the understanding of chromosome architecture. Chromosome Architecture: Methods and Protocols guides readers through cutting-edge interdisciplinary methods which allow for an understanding of architecture of chromosomes with exceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromosome Architecture: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Download or read book Glycoprotein Methods and Protocols written by Anthony P. Corfield and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: The mucins (mucus glycoproteins) have long been a complex corner of glycoprotein biology. While dramatic advances in the separation, structural an- ysis, biosynthesis, and degradation have marked the progress in general glycop- tein understanding, the mucins have lagged behind. The reasons for this lack of progress have always been clear and are only now being resolved. The mucins are very large molecules; they are difficult to separate from other molecules present in mucosal secretions or membranes; they are often degraded owing to natural protective functions or to isolation methodology and their peptide and oligos- charide structures are varied and complex. Understanding these molecules has demanded progress in several major areas. Isolation techniques that protect the intact mucins and allow dissociation from other adsorbed but discrete molecules needed to be developed and accepted by all researchers in the field. Improved methods for the study of very large molecules with regard to their aggregation and polymerization were also needed. Structural analysis of the peptide domains and the multitude of oligosaccharide chains was required for smaller sample sizes, for multiple samples, and in shorter time. In view of these problems it is perhaps not surprising that the mucins have remained a dilemma, of obvious biological importance and interest, but very difficult to analyze.
Download or read book Bacterial Chromatin written by Remus T. Dame and published by Springer Nature. This book was released on with total page 654 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chromosome Architecture written by Mark C. Leake and published by Springer Nature. This book was released on 2022-05-30 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investigation, manifest both through application of more complex bottom-up assays in vitro as well as through maintaining the native physiological context through the investigation of living, functional cells. In addition, the chapters reflect the dramatic improvement in the length scale of precision by utilizing single-molecule approaches, both for imaging the DNA content of chromosome and proteins that bind to DNA as well as using methods that can controllably manipulate single DNA molecules, and the use of advanced computational methods and mathematical analysis is also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromosome Architecture: Methods and Protocols, Second Edition is an ideal guide for researchers working in this dynamic area of study.
Download or read book Spatial Genome Organization written by Tom Sexton and published by Springer Nature. This book was released on 2022-07-22 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the heterogeneity and dynamics of nuclear architectures and approaches to visualize them by microscopy. Finally, in order to be able to ask functional questions about the role of spatial chromatin organization in genomic control, the last section provides methods for acute manipulations of chromatin architecture. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Spatial Genome Organization: Methods and Protocols is an ideal resource for researchers searching for the best techniques to address their own specific research questions.
Download or read book Protein Protein Interactions written by Shahid Mukhtar and published by Springer Nature. This book was released on 2023-07-14 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume provides a comprehensive collection of classic and cutting-edge methods and techniques in mapping protein-protein interactions. The chapters include a variety of in vitro and in vivo experimental methods covering cell biology, biochemistry, and biophysics. In addition, the book also explores in silico methods including sequence-, structure-, and phylogenetic profile-based approaches as well as gene expression and machine learning methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein-Protein Interactions: Methods and Protocols serves as an ideal guide for researchers working in protein science and beyond.
Download or read book Methods and Applications for Modeling and Simulation of Complex Systems written by Fazilah Hassan and published by Springer Nature. This book was released on 2023-10-12 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book constitutes the refereed proceedings of the 22nd Asia Simulation Conference on Methods and Applications for Modeling and Simulation of Complex Systems, AsiaSim 2023, held in Langkawi, Malaysia, during October 25–26, 2023. The 77 full papers included in this book were carefully reviewed and selected from 164 submissions. They were organized in topical sections as follows: Modelling and Simulation, Artificial intelligence, Industry 4.0, Digital Twins Modelling, Simulation and Gaming, Simulation for Engineering, Simulation for Sustainable Development, Simulation in Social Sciences.
Download or read book Prokaryotic Gene Regulation written by Eveline Peeters and published by Springer Nature. This book was released on 2022-08-03 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both. The chapters in this book are divided into four sections. Section One covers methods that enable the study of the structure of the bacterial/archaeal chromosome, the main template for all gene regulatory processes, and its epigenetic modification. Section Two looks at a selection of approaches that enable higher levels of understanding of transcription initiation, a key step in information processing. Section Three discusses the investigation of regulating transcription factors, which are often considered the main players in gene regulation in prokaryotic cells. The Fourth Section focuses on the next stage of information processing at which gene regulation occurs, namely the RNA-based level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Prokaryotic Gene Regulation: Methods and Protocols is a valuable resource for researchers interested in learning more about this diverse field.
Download or read book Applications of Secure Multiparty Computation written by P. Laud and published by IOS Press. This book was released on 2015-07-30 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: We generate and gather a lot of data about ourselves and others, some of it highly confidential. The collection, storage and use of this data is strictly regulated by laws, but restricting the use of data often limits the benefits which could be obtained from its analysis. Secure multi-party computation (SMC), a cryptographic technology, makes it possible to execute specific programs on confidential data while ensuring that no other sensitive information from the data is leaked. SMC has been the subject of academic study for more than 30 years, but first attempts to use it for actual computations in the early 2000s – although theoretically efficient – were initially not practicable. However, improvements in the situation have made possible the secure solving of even relatively large computational tasks. This book describes how many different computational tasks can be solved securely, yet efficiently. It describes how protocols can be combined to larger applications, and how the security-efficiency trade-offs of different components of an SMC application should be chosen. Many of the results described in this book were achieved as part of the project Usable and Efficient Secure Multi-party Computation (UaESMC), which was funded by the European Commission. The book will be of interest to all those whose work involves the secure analysis of confidential data.
Download or read book Sharing Clinical Trial Data written by Institute of Medicine and published by National Academies Press. This book was released on 2015-04-20 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Data sharing can accelerate new discoveries by avoiding duplicative trials, stimulating new ideas for research, and enabling the maximal scientific knowledge and benefits to be gained from the efforts of clinical trial participants and investigators. At the same time, sharing clinical trial data presents risks, burdens, and challenges. These include the need to protect the privacy and honor the consent of clinical trial participants; safeguard the legitimate economic interests of sponsors; and guard against invalid secondary analyses, which could undermine trust in clinical trials or otherwise harm public health. Sharing Clinical Trial Data presents activities and strategies for the responsible sharing of clinical trial data. With the goal of increasing scientific knowledge to lead to better therapies for patients, this book identifies guiding principles and makes recommendations to maximize the benefits and minimize risks. This report offers guidance on the types of clinical trial data available at different points in the process, the points in the process at which each type of data should be shared, methods for sharing data, what groups should have access to data, and future knowledge and infrastructure needs. Responsible sharing of clinical trial data will allow other investigators to replicate published findings and carry out additional analyses, strengthen the evidence base for regulatory and clinical decisions, and increase the scientific knowledge gained from investments by the funders of clinical trials. The recommendations of Sharing Clinical Trial Data will be useful both now and well into the future as improved sharing of data leads to a stronger evidence base for treatment. This book will be of interest to stakeholders across the spectrum of research-from funders, to researchers, to journals, to physicians, and ultimately, to patients.
Download or read book Lecture Notes in Real Time Intelligent Systems written by Jolanta Mizera-Pietraszko and published by Springer. This book was released on 2018-05-14 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second volume of the book series highlights works presented at the 2nd International Conference on Real Time Intelligent Systems, held in Casablanca on October 18-20, 2017. The book offers a comprehensive, practical review of the state-of-the-art in designing and implementing real-time intelligent computing for the areas within the conference’s scope such as robotics, intelligent alert systems, IoT, remote access control, multi-agent systems, networking, mobile smart systems, crowdsourcing, broadband systems, cloud computing, streaming data and many other applications. Research in real-time computing supports decision making in dynamic environments. Some examples include ABS, FBW flight control, automatic air-conditioning, etc. Intelligent computing relies heavily on artificial intelligence (AI) to make computers act for humans. The authors are confident that the solutions discussed in this book will provide a unique source of information and inspiration for researchers working in AI, distributed coding algorithms or smart services and platforms, and for IT professionals, who can integrate the proposed methods into their practice.
Download or read book Protocol Based Sliding Mode Control written by Jun Song and published by CRC Press. This book was released on 2022-09-14 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the Sliding Mode Control (SMC) problems of networked control systems (NCSs) under various communication protocols including static/dynamic/periodic event-triggered mechanism, and stochastic communication, Round-Robin, weighted try-once-discard, multiple-packet transmission, and the redundant channel transmission protocol. The super-twisting algorithm and the extended-state-observer-based SMC scheme are described in this book for suppressing chattering. Besides, the SMC designs for two-dimensional (1-D) and two-dimensional (2-D) NCSs are illustrated as well. Features: Captures recent advances of theories, techniques, and applications of networked sliding mode control from an engineering-oriented perspective. Includes new design ideas and optimization techniques of networked sliding mode control theory. Provides advanced tools to apply networked sliding mode control techniques in the practical applications. Discusses some new tools to the engineering applications while dealing with the model uncertainties and external disturbances. This book aims at Researchers and professionals in Control Systems, Computer Networks, Internet of Things, and Communication Systems.
Download or read book Typologies and Taxonomies written by Kenneth D. Bailey and published by SAGE. This book was released on 1994-06-13 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: How do we group different subjects on a variety of variables? Should we use a classification procedure in which only the concepts are classified (typology), one in which only empirical entities are classified (taxonomy), or some combination of both? In this clearly written book, Bailey addresses these questions and shows how classification methods can be used to improve research. Beginning with an exploration of the advantages and disadvantages of classification procedures including those typologies that can be constructed without the use of a computer, the book covers such topics as clustering procedures (including agglomerative and divisive methods), the relationship among various classification techniques (including the relationship of monothetic, qualitative typologies to polythetic, quantitative taxonomies), a comparison of clustering methods and how these methods compare with related statistical techniques such as factor analysis, multidimensional scaling and systems analysis, and lists classification resources. This volume also discusses software packages for use in clustering techniques.
Download or read book NASA Formal Methods written by Guillaume Brat and published by Springer. This book was released on 2013-05-09 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book constitutes the refereed proceedings of the 5th International Symposium on NASA Formal Methods, NFM 2013, held in Moffett Field, CA, USA, in May 2013. The 28 revised regular papers presented together with 9 short papers talks were carefully reviewed and selected from 99 submissions. The topics are organized in topical sections on model checking; applications of formal methods; complex systems; static analysis; symbolic execution; requirements and specifications; probabilistic and statistical analysis; and theorem proving.
Download or read book Single Molecule Analysis written by Iddo Heller and published by Springer Nature. This book was released on 2023-11-13 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book Federated Learning written by Yaochu Jin and published by Springer Nature. This book was released on 2022-11-29 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces readers to the fundamentals of and recent advances in federated learning, focusing on reducing communication costs, improving computational efficiency, and enhancing the security level. Federated learning is a distributed machine learning paradigm which enables model training on a large body of decentralized data. Its goal is to make full use of data across organizations or devices while meeting regulatory, privacy, and security requirements. The book starts with a self-contained introduction to artificial neural networks, deep learning models, supervised learning algorithms, evolutionary algorithms, and evolutionary learning. Concise information is then presented on multi-party secure computation, differential privacy, and homomorphic encryption, followed by a detailed description of federated learning. In turn, the book addresses the latest advances in federate learning research, especially from the perspectives of communication efficiency, evolutionary learning, and privacy preservation. The book is particularly well suited for graduate students, academic researchers, and industrial practitioners in the field of machine learning and artificial intelligence. It can also be used as a self-learning resource for readers with a science or engineering background, or as a reference text for graduate courses.
