Download or read book Single Molecule Tools Part A Fluorescence Based Approaches written by and published by Academic Press. This book was released on 2010-08-17 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. - Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Download or read book Label Free Biosensing written by Michael J. Schöning and published by Springer. This book was released on 2018-07-20 with total page 485 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume summarizes the state-of-the-art technologies, key advances and future trends in the field of label-free biosensing. It provides detailed insights into the different types of solid-state, label-free biosensors, their underlying transducer principles, advanced materials utilized, device-fabrication techniques and various applications. The book offers graduate students, academic researchers, and industry professionals a comprehensive source of information on all facets of label-free biosensing and the future trends in this flourishing field. Highlights of the subjects covered include label-free biosensing with: · semiconductor field-effect devices such as nanomaterial-modified capacitive electrolyte-insulator-semiconductor structures, silicon nanowire transistors, III-nitride semiconductor devices and light-addressable potentiometric sensors · impedimetric biosensors using planar and 3D electrodes · nanocavity and solid-state nanopore devices · carbon nanotube and graphene/graphene oxide biosensors · electrochemical biosensors using molecularly imprinted polymers · biomimetic sensors based on acoustic signal transduction · enzyme logic systems and digital biosensors based on the biocomputing concept · heat-transfer as a novel transducer principle · ultrasensitive surface plasmon resonance biosensors · magnetic biosensors and magnetic imaging devices

Download or read book Single Molecule Analysis written by Iddo Heller and published by Springer Nature. This book was released on 2023-11-13 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by OUP Oxford. This book was released on 2006-08-17 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.

Download or read book Single Molecule Science written by Krishnarao Appasani and published by . This book was released on 2022-05-11 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.

Download or read book Single Molecules and Nanotechnology written by Rudolf Rigler and published by Springer Science & Business Media. This book was released on 2007-12-07 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on recent advances in the rapidly evolving field of single molecule research. These advances are of importance for the investigation of biopolymers and cellular biochemical reactions, and are essential to the development of quantitative biology. Written by leading experts in the field, the articles cover a broad range of topics, including quantum photonics of organic dyes and inorganic nanoparticles and monitoring of single molecule (enzymatic) reactions.

Download or read book Introduction to Fluorescence Microscopy written by J. S. Ploem and published by Garland Science. This book was released on 1987 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers three main areas.The phenomenon of fluorescence, the main applications, and the most common problems.

Download or read book Optical Fluorescence Microscopy written by Alberto Diaspro and published by Springer Science & Business Media. This book was released on 2010-11-25 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Download or read book Single Molecule Cellular Biophysics written by Mark C. Leake and published by Cambridge University Press. This book was released on 2013-01-31 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: Indispensable textbook for undergraduate students in the physical and life sciences, unravelling the inner workings of the cell.

Download or read book Who s Who in Fluorescence 2009 written by Chris D. Geddes and published by Springer Science & Business Media. This book was released on 2009-04-02 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: th The Who’s Who in Fluorescence 2009 is the 7 volume of the Who’s who series. The previous six volumes (2003 – 2008) have been very well received by the fluorescence community, with 1000’s of copies being distributed around the world, through conferences and workshops, as well as through internet book sites. In addition, the Institute of Fluorescence (http://theinstituteoffluorescence.com/) mailed 100’s of copies of the 2008 volume to contributors around the world. This new 2009 volume features some 419 entries from no fewer than 41 countries worldwide, as compared to 418 entries (38 different countries) in 2008 and 405 entries in the 2007 volume, respectively. We have received 29 new entries this year, and deleted 25 entries that were not updated by contributors from past years. In 2008, 129 AIM numbers were submitted as compared to 106 in 2007. This year the number has risen again to 136 AIM numbers submitted. This year we also see the introduction of the h-index number listing, a publication statistic provided by the Thompson’s ISI Web of Science. Some 42 contributors provided their h-numbers. In 2009 we also see a continued and strong company support, in light of the current world economic climate, which will enable us to further disseminate the volume in 2009– 2010. In this regard we especially thank the instrumentation companies for their continued support, where without their financial contributions, it is likely that the volume would not be the success it is today.

Download or read book Development of Single Molecule Optical Techniques for the Study of Recognition and Repair of DNA Damage written by Samantha R. Fore and published by . This book was released on 2006 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book An Introductory Text To Bioengineering written by Shu Chien and published by World Scientific Publishing Company. This book was released on 2008-05-27 with total page 563 pages. Available in PDF, EPUB and Kindle. Book excerpt: This bestselling textbook will introduce undergraduate bioengineering students to the fundamental concepts and techniques, with the basic theme of integrative bioengineering. It covers bioengineering of several body systems, organs, tissues, and cells, integrating physiology at these levels with engineering concepts and approaches; novel developments in tissue engineering, regenerative medicine, nanoscience and nanotechnology; state-of-the-art knowledge in systems biology and bioinformatics; and socio-economic aspects of bioengineering.One of the distinctive features of the book is that it is integrative in nature (integration of biology, medicine and engineering, across different levels of the biological hierarchy, and basic knowledge with applications). It is unique in that it covers fundamental aspects of bioengineering, cutting-edge frontiers, and practical applications, as well as perspectives of bioengineering development. Furthermore, it covers important socio-economical aspects of bioengineering such as ethics and entrepreneurism.

Download or read book Chemical Biology written by Banafshe Larijani and published by John Wiley & Sons. This book was released on 2006-08-04 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by a team of international researchers and teachers at the cutting edge of chemical biology research, this book provides an exciting, comprehensive introduction to a wide range of chemical and physical techniques with applications in areas as diverse as molecular biology, signal transduction, drug discovery and medicine. Techniques include: Cryo-electron microscopy, atomic force microscopy, differential scanning calorimetry in the study of lipid structures, membrane potentials and membrane probes, identification and quantification of lipids using mass spectroscopy, liquid state NMR, solid state NMR in biomembranes, molecular dynamics, two dimensional infra-red studies of biomolecules, single and two-photon fluorescence, optical tweezers, PET imaging and chemical genetics. KEY FEATURES: a unique guide to the rapidly evolving, interdisciplinary field of chemical biology. adopts a molecular structure for maximum flexibility. addresses relevant, topical chemical biological questions throughout. includes stunning illustrations. associates website with PowerPoint slides of figures within the book. Chemical Biology: Techniques and Applications provides an invaluable resource for final year undergraduate and post graduate bioscience and biomedical students and pharmaceutical researchers with an interest in this fascinating, and ever changing field.

Download or read book Myosins written by Lynne M. Coluccio and published by Springer Nature. This book was released on 2020-05-25 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly authoritative volume highlights the remarkable superfamily of molecular motors called myosins, which are involved in such diverse cellular functions as muscle contraction, intracellular transport, cell migration and cell division. In a timely compilation of chapters written by leading research groups that have made key discoveries in the field, the current understanding of the molecular mechanisms and biological functions of these intriguing proteins is explored.

Download or read book High Resolution Imaging in Microscopy and Ophthalmology written by Josef F. Bille and published by Springer. This book was released on 2019-08-13 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.

Download or read book Nanoscopy and Multidimensional Optical Fluorescence Microscopy written by Alberto Diaspro and published by CRC Press. This book was released on 2010-04-26 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Alberto Diaspro has been choreographing light's dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology's secrets."- From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimens

Download or read book Fluorescent Proteins written by Kevin F. Sullivan and published by Elsevier. This book was released on 2007-12-14 with total page 613 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.