Download or read book Self induced Back Action Actuated Nanopore Electrophoresis SANE Sensor for Molecular Detection and Analysis written by Sai Santosh Sasank Peri and published by . This book was released on 2021 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt: We fabricated a novel single molecule nanosensor by integrating a Solid-State Nanopore (SSNP) and a Double Nanohole (DNH) nanoaperture. The nanosensor employs Self-Induced Back-Action (SIBA) for optical trapping and enables SIBA-Actuated Nanopore Electrophoresis (SANE) for concurrent acquisition of bimodal optical and electrical signatures of molecular interactions. We demonstrated the potential utility of the SANE sensor by trapping and translocating 20 nm silica and gold nanoparticles. The electrical translocation time of the nanoparticles was extended by four orders of magnitude due to opposing electrical and optical forces acting on the nanoparticle, causing high frequency oscillations or bobbing in the electrical signal. Using frequency analysis, we were able to show that bobbing can be used as a signature to distinguish between single and multiple trapping. These promising results enabled us to pursue biomolecular detection with SANE sensor. We used high affinity T-cell receptor-like antibodies (TCRmAbs), and tested their binding to specific peptide-presenting Major Histocompatibility Complex (pMHC) ligands. We used irrelevant TCRmAbs, targeting the same pMHCs as control experiments. We were able to distinguish between individual molecules and their specific and non-specific mixtures. The optical-electrical metrics enabled measurement of increased bound fraction of the antibody-ligand complexes at lower concentrations than bulk solution equilibrium binding constant (KD). In addition, we detected low affinity ligand-receptor interactions between soluble heterodimer receptors and pMHC ligands. We used irrelevant pMHCs to target the same receptor as a control experiment. We discriminated the optical-electrical signatures for specific and non-specific binding of receptor-ligand interactions, and were able to quantify the dissociation rate constant (koff) of the receptor-ligand binding comparable to the commercial technologies. The measurement koff value can be correlated to the receptor-ligand binding time required for activation of immune response in vivo. Therefore, we demonstrated the utility of SANE sensor as a potential screening tool in cancer immunotherapy.
Download or read book Single Molecule Sensing Beyond Fluorescence written by Warwick Bowen and published by Springer Nature. This book was released on 2022-03-01 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an interesting snapshot of recent advances in the field of single molecule nanosensing. The ability to sense single molecules, and to precisely monitor and control their motion is crucial to build a microscopic understanding of key processes in nature, from protein folding to chemical reactions. Recently a range of new techniques have been developed that allow single molecule sensing and control without the use of fluorescent labels. This volume provides an overview of recent advances that take advantage of micro- and nanoscale sensing technologies and provide the prospect for rapid future progress. The book endeavors to provide basic introductions to key techniques, recent research highlights, and an outlook on big challenges in the field and where it will go in future. It is a valuable contribution to the field of single molecule nanosensing and it will be of great interest to graduates and researchers working in this topic.
Download or read book Nanopore Biosensors for Molecular Detection and Analysis written by Muhammad Usman Raza and published by . This book was released on 2019 with total page 109 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanoscale fabrication techniques have led the march towards a new class of biosensors that were not possible to build in the past due to technological limitations. These molecular nanosensors can have biochemical, electromagnetic, acoustic, optical or electrophoretic detection modalities. One of these nanosensors, is solid state nanopores which use resistive pulse sensing in nanometer apertures in thin dielectric membranes. Solid state nanopores have been fabricated using cutting edge fabrication techniques to detect and analyze nanoscale biomolecules such as DNA, proteins, miRNA, viruses etc. These biosensors can be used as electronic sensors for biomarkers of certain diseases such as cancer and can form effective tools for early detection which can eventually save lives. Solid state nanopores have also been used as a novel way for cheap DNA sequencing. In the case for early detection of cancer, molecular biology plays a critical role and solid state nanopores can be used to study that and provide effective ways for the effect. In this dissertation we work on solid state nanopores and try to improve them using the cutting-edge fabrication techniques and optical modalities to make them ultrasensitive biomarker detection and multiplexed sensing biosensors. In the first project, we use solid state nanopores in silicon nitride membranes to detect epidermal growth factor receptor (EGFR) protein which is overexpressed in many cancers and is a cancer biomarker. We use an RNA Aptamer to selectively bind to the EGFR which increases the selectivity for this cancer biomarker through solid state nanopores. In the second project, we simulate two adjacent nanopores on a single membrane in the quest to find the adjacent nanopore distance for reduced electronic crosstalk. The optical parameters derived from the simulations can be used as a blue print for fabrication of nanopore arrays on single membranes to increase the throughput and self-referencing capability for noise cancellation. The final project reinvents the solid state nanopore with the inclusion of an optical plasmonic trap cavity at the mouth of the nanopore. We are the first ones to report this type of nanopore device. This novel sensor provides dual mode optical and electrical sensing capability. In addition, the localized plasmon field can trap the nanoparticles for up to 7 seconds at the mouth of the nanopore, which was previously not possible due to the electrophoretic translocation. It is shown that nanoparticles oscillate in the trap, which results in high frequency electrical noise that is synchronous to the mass loading of the plasmonic cavity.
Download or read book The Grape Genome written by Dario Cantu and published by Springer Nature. This book was released on 2019-11-13 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the current state of international grape genomics, with a focus on the latest findings, tools and strategies employed in genome sequencing and analysis, and genetic mapping of important agronomic traits. It also discusses how these are having a direct impact on outcomes for grape breeders and the international grape research community. While V. vinifera is a model species, it is not always appreciated that its cultivation usually requires the use of other Vitis species as rootstocks. The book discusses genetic diversity within the Vitis genus, the available genetic resources for breeding, and the available genomic resources for other Vitis species. Grapes (Vitis vinifera spp. vinifera) have been a source of food and wine since their domestication from their wild progenitor (Vitis vinifera ssp. sylvestris) around 8,000 years ago, and they are now the world’s most valuable horticultural crop. In addition to being economically important, V. vinifera is also a model organism for the study of perennial fruit crops for two reasons: Firstly, its ability to be transformed and micropropagated via somatic embryogenesis, and secondly its relatively small genome size of 500 Mb. The economic importance of grapes made V. vinifera an obvious early candidate for genomic sequencing, and accordingly, two draft genomes were reported in 2007. Remarkably, these were the first genomes of any fruiting crop to be sequenced and only the fourth for flowering plants. Although riddled with gaps and potentially omitting large regions of repetitive sequences, the two genomes have provided valuable insights into grape genomes. Cited in over 2,000 articles, the genome has served as a reference in more than 3,000 genome-wide transcriptional analyses. Further, recent advances in DNA sequencing and bioinformatics are enabling the assembly of reference-grade genome references for more grape genotypes revealing the exceptional extent of structural variation in the species.
Download or read book Engineered Nanopores for Bioanalytical Applications written by Joshua B. Edel and published by William Andrew. This book was released on 2013-03-19 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding.This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices. - Includes application case studies for detection, identification and analysis of biomolecules and related functional nanomaterials - Introduces the techniques of manufacturing solid state materials with functional nanopores - Explains the use of nanopores in DNA sequencing and the wider range of applications from environmental monitoring to forensics
Download or read book Handbook of Digital Innovation written by Satish Nambisan and published by Edward Elgar Publishing. This book was released on 2020-07-31 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Digital innovations influence every aspect of life in an increasingly digitalized world. Firms pursuing digital innovations must consider how digital technologies shape the nature, process and outcomes of innovation as well as long- and short-term social, economic and cultural consequences of their offerings. This Handbook contributes to a transdisciplinary understanding of digital innovation with a diverse set of leading scholars and their distinct perspectives. The ideas and principles advanced herein set the agenda for future transdisciplinary research on digital innovation in ways that inform not only firm-level strategies and practices but also policy decisions and science-focused investments.
Download or read book Quality by Design for Biopharmaceutical Drug Product Development written by Feroz Jameel and published by Springer. This book was released on 2015-04-01 with total page 710 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the application of Quality by Design (QbD) to biopharmaceutical drug product development. Twenty-eight comprehensive chapters cover dosage forms, liquid and lyophilized drug products. The introductory chapters of this book define key elements of QbD and examine how these elements are integrated into drug product development. These chapters also discuss lessons learned from the FDA Office of Biotechnology Products pilot program. Following chapters demonstrate how QbD is used for formulation development ranging from screening of formulations to developability assessment to development of lyophilized and liquid formats. The next few chapters study the use of small-scale and surrogate models as well as QbD application to drug product processes such as drug substance freezing and thawing, mixing, sterile filtration, filling, lyophilization, inspection and shipping and handling. Later chapters describe more specialized applications of QbD in the drug product realm. This includes the use of QbD in primary containers, devices and combination product development. The volume also explores QbD applied to vaccine development, automation, mathematical modeling and monitoring, and controlling processes and defining control strategies. It concludes with a discussion on the application of QbD to drug product technology transfer as well as overall regulatory considerations and lifecycle management. Quality by Design for Biopharmaceutical Drug Product Development is an authoritative resource for scientists and researchers interested in expanding their knowledge on QbD principles and uses in creating better drugs.
Download or read book Nanotechnology in Drug Delivery written by Melgardt M. de Villiers and published by Springer Science & Business Media. This book was released on 2008-10-29 with total page 681 pages. Available in PDF, EPUB and Kindle. Book excerpt: The reader will be introduced to various aspects of the fundamentals of nanotechnology based drug delivery systems and the application of these systems for the delivery of small molecules, proteins, peptides, oligonucleotides and genes. How these systems overcome challenges offered by biological barriers to drug absorption and drug targeting will also be described.
Download or read book Nano Medicine and Nano Safety written by Malay K. Das and published by Springer Nature. This book was released on 2020-12-09 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the application of Nanobiotechnology in the development of Nanomedicine, while also discussing the latest trends and challenges in the clinical translation of Nanomedicine. Nanomedicine refers to the application of Nanotechnology to medicine and holds tremendous potential for achieving improved efficiency, bioavailability, dose response, personalized medicine and enhanced safety as compared to conventional medicines. The book first introduces readers to the basic concepts of Nanomedicine, and to technological advances in and applications of Nanotechnology in treatment, diagnosis, monitoring, and drug delivery. In turn, it reviews the current status of multi-functionalization strategies for using Nanoparticles in the targeted delivery of therapeutic agents. The book’s third and final section focuses on the regulatory and safety challenges posed by Nanomedicine, including industry and regulatory agencies’ efforts to address them.
Download or read book Environmental Biotechnology written by Jeyabalan Sangeetha and published by CRC Press. This book was released on 2016-10-14 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: With focus on the practical use of modern biotechnology for environmental sustainability, this book provides a thoughtful overview of molecular aspects of environmental studies to create a new awareness of fundamental biological processes and sustainable ecological concerns. It covers the latest research by prominent scientists in modern biology and delineates recent and prospective applications in the sub-areas of environmental biotechnology with special focus on the biodegradation of toxic pollutants, bioremediation of contaminated environments, and bioconversion of organic wastes toward a green economy and sustainable future.
Download or read book Processing of Foods and Biomass Feedstocks by Pulsed Electric Energy written by Eugene Vorobiev and published by Springer Nature. This book was released on 2020-04-10 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive range of research on pulsed electric energy used in food processing, including sections on the fundamentals of electroporation and important techniques for the estimation of electroporation effects in various foods and biomass feedstocks. By focusing on application over theory, this book presents researchers with practical steps for processing techniques such as solid-liquid extraction, pressing, osmotic dehydration, drying, freezing and cooking. Special interest is given to the selective recovery and extraction of sugar, inulin, starch, proteins, polysaccharides, polyphenols, pigments, flavor compounds, phytochemicals and other of high-value components from food biomasses such as fruits and vegetables, leaves, herbs, mushrooms, microalgae and suspensions of cells. Processing of Foods and Biomass Feedstocks by Pulsed Electric Energy presents a singular overview of the biorefinery applications of pulsed electric energy for the processing of wastes and non-food biomasses such as root and tuber crops, grape waste, lignocellulosic biomass, oil crops and residues and seeds and peels of exotic and citrus fruits. The book begins by presenting general information on the fundamentals of electroporation and information on the procedures and protocols involved. Further chapters focus on the specific food processing operations involved and biorefinery applications for the processing of wastes and non-food biomasses. All of the relevant and up-to-date information any researcher needs on pulsed electric energy in food processing is presented here in this text.
Download or read book Advances in Bioengineering written by Renu Vyas and published by Springer Nature. This book was released on 2020-05-11 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a single source of information on three major bioengineering areas: engineering at the cellular and molecular level; biomedical devices / instrument engineering; and data engineering. It explores the latest strategies that are essential to advancing our understanding of the mechanisms of human diseases, the development of new enzyme-based technologies, diagnostics, prosthetics, high-performance computing platforms for managing huge amounts of biological data, and the use of deep learning methods to create predictive models. The book also highlights the growing importance of integrating chemistry into life sciences research, most notably concerning the development and evaluation of nanomaterials and nanoparticles and their interactions with biological material. The underlying interdisciplinary theme of bioengineering is addressed in a range of multifaceted applications and worked out examples provided in each chapter.
Download or read book Nanopores written by Samir M. Iqbal and published by Springer Science & Business Media. This book was released on 2011-07-12 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanopores are nanometer scale holes formed naturally by proteins or cells, and can be used for a variety of applications, including sequencing DNA and detecting anthrax. They can be integrated into artificially constructed encapsulated cells of silicon wafers while allowing small molecules like oxygen, glucose and insulin to pass, while keeping out large system molecules. "Nanopores: Sensing and Fundamental Biological Interactions" examines the emerging research directions surrounding nanopores such as genome sequencing and early disease detection using biomarker identification. Covering the applications of nanopores in genetics, proteomics, drug discovery, early disease detection and detection of emerging environmental threats, it is a must-have book for biomedicalengineers and research scientists.
Download or read book Lab on Fiber Technology written by Andrea Cusano and published by Springer. This book was released on 2014-07-29 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on a research field that is rapidly emerging as one of the most promising ones for the global optics and photonics community: the “lab-on-fiber” technology. Inspired by the well-established "lab on-a-chip" concept, this new technology essentially envisages novel and highly functionalized devices completely integrated into a single optical fiber for both communication and sensing applications. Based on the R&D experience of some of the world's leading authorities in the fields of optics, photonics, nanotechnology, and material science, this book provides a broad and accurate description of the main developments and achievements in the lab-on-fiber technology roadmap, also highlighting the new perspectives and challenges to be faced. This book is essential for scientists interested in the cutting-edge fiber optic technology, but also for graduate students.
Download or read book Microbiorobotics written by Minjun Kim and published by William Andrew. This book was released on 2012-03-08 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbiorobotics is a new engineering discipline that inherently involves a multidisciplinary approach (mechanical engineering, cellular biology, mathematical modeling, control systems, synthetic biology, etc). Building robotics system in the micro scale is an engineering task that has resulted in many important applications, ranging from micromanufacturing techniques to cellular manipulation. However, it is also a very challenging engineering task. One of the reasons is because many engineering ideas and principles that are used in larger scales do not scale well to the micro-scale. For example, locomotion principles in a fluid do not function in the same way, and the use of rotational motors is impractical because of the difficulty of building of the required components. Microrobotics is an area that is acknowledged to have massive potential in applications from medicine to manufacturing. This book introduces an inter-disciplinary readership to the toolkit that micro-organisms offer to micro-engineering The design of robots, sensors and actuators faces a range of techology challenges at the micro-scale. This book shows how biological techniques and materials can be used to meet these challenges World-class multi-disciplanry editors and contributors leverage insights from engineering, mathematical modeling and the life sciences – creating a novel toolkit for microrobotics
Download or read book Thermoplasmonics written by Guillaume Baffou and published by Cambridge University Press. This book was released on 2017-10-19 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plasmonics is an important branch of optics concerned with the interaction of metals with light. Under appropriate illumination, metal nanoparticles can exhibit enhanced light absorption, becoming nanosources of heat that can be precisely controlled. This book provides an overview of the exciting new field of thermoplasmonics and a detailed discussion of its theoretical underpinning in nanophotonics. This topic has developed rapidly in the last decade, and is now a highly-active area of research due to countless applications in nanoengineering and nanomedicine. These important applications include photothermal cancer therapy, drug and gene delivery, nanochemistry and photothermal imaging. This timely and self-contained text is suited to all researchers and graduate students working in plasmonics, nano-optics and thermal-induced processes at the nanoscale.
Download or read book tudes Sur Les Antiquit s Juridiques D Ath nes Le Cr dit Foncier Ath nes written by Exupère Caillemer and published by Legare Street Press. This book was released on 2022-10-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work is in the "public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.
