Download or read book RV Primer written by Michael T Vreeland & Marcia N Vreeland and published by Dorrance Publishing. This book was released on 2021-05-13 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt: RV Primer - How to Start and Enjoy RVing By: Michael Vreeland In 2008 Michael and Marcy Vreeland started RVing and in 2017 sold their home and began the adventure of full-time RVing. Over the years they have learned valuable lessons about this unique lifestyle while trying to help other RVers avoid the mistakes they have made. They wrote RV Primer to give those just starting out tips and tricks to avoid the mistakes they had made. Readers who are beginner RVers will gain valuable insight that will save them time and money while they enjoy the Vreeland’s story-filled, humorous prose. Among the topics covered are how to select the right RV, how to properly maintain your RV, and how to wisely stock your RV. Whether you are considering RVing or are merely interested in what it is like, RV Primer is the go-to book.
Download or read book The Nucleic Acid Protocols Handbook written by Ralph Rapley and published by Springer Science & Business Media. This book was released on 2008-06-29 with total page 997 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Download or read book Prokaryotic Gene Regulation written by Eveline Peeters and published by Springer Nature. This book was released on 2022-08-03 with total page 321 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both. The chapters in this book are divided into four sections. Section One covers methods that enable the study of the structure of the bacterial/archaeal chromosome, the main template for all gene regulatory processes, and its epigenetic modification. Section Two looks at a selection of approaches that enable higher levels of understanding of transcription initiation, a key step in information processing. Section Three discusses the investigation of regulating transcription factors, which are often considered the main players in gene regulation in prokaryotic cells. The Fourth Section focuses on the next stage of information processing at which gene regulation occurs, namely the RNA-based level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Prokaryotic Gene Regulation: Methods and Protocols is a valuable resource for researchers interested in learning more about this diverse field.
Download or read book Polyploidy and Hybridization for Crop Improvement written by Annaliese S. Mason and published by CRC Press. This book was released on 2017-11-22 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many of our current agricultural crops are natural or agricultural hybrids (between two or more species), or polyploids (containing more than one genome or set of chromosomes). These include potato, oats, cotton, oilseed rape, wheat, strawberries, kiwifruit, banana, seedless watermelon, triticale and many others. Polyploidy and hybridization can also be used for crop improvement: for example, to introgress disease resistance from wild species into crops, to produce seedless fruits for human consumption, or even to create entirely new crop types. Some crop genera have hundreds of years of interspecific hybridization and ploidy manipulation behind them, while in other genera use of these evolutionary processes for crop improvement is still at the theoretical stage. This book brings together stories and examples by expert researchers and breeders working in diverse crop genera, and details how polyploidy and hybridization processes have shaped our current crops, how these processes have been utilized for crop improvement in the past, and how polyploidy and interspecific hybridization can be used for crop improvement in the future.
Download or read book Celebrating Twenty Years of the Brazilian Symposium on Cardiovascular Physiology written by Camille M. Balarini and published by Frontiers Media SA. This book was released on 2017-06-09 with total page 243 pages. Available in PDF, EPUB and Kindle. Book excerpt: This e-book is dedicated to the celebration of 20 years of the Brazilian Symposium on Cardiovascular Physiology. In 1996 groups from the School of Medicine of Ribeirao Preto, University of Sao Paulo (FMRP-USP) and from the Federal University of Sao Paulo (UNIFESP) joined together to discuss cardiovascular physiology. In subsequent editions of the meeting, the participation of other groups from all over the country has grown and acquired the status of a national symposium. The participants now agree that the symposium should be itinerant and that the chair group is responsible for its organization. In 2016, we proudly reached the 20th edition of the Brazilian Symposium on Cardiovascular Physiology. It is certainly a memorable date and a great opportunity to share the accomplishments of Brazilian groups in the field of cardiovascular physiology.
Download or read book Renal Cancer written by Jack H. Mydlo and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of Renal Cancer: Methods and Protocols is to introduce the surgeon, clinician, investigator, and research scientist to the basic methods employed in the diagnosis and treatment of renal cancer. Treatment of localized renal cancer is surgical. Treatment of metastasis with resection, radiotherapy, chemotherapy, and immunotherapy has had limited results. Therefore, new avenues of treatment are necessary. In planning this work, I have attempted to incorporate coverage by specialists from a variety of disciplines, each applying their individual expertise in renal cancer therapy. Though many of the participating authors are urologists, there are also valuable contributions from medical oncologists, laboratory inv- tigators, and pathologists. One of the inherent problems in publishing a book that describes molecular techniques, especially those techniques that are rapidly evolving, is that some of these may become obsolete after a few years. However, many of the procedures detailed here are standard approaches that have already wi- stood the test of time. Reverse transcriptase PCR, or RT-PCR, a technique that produces m- tiple copies of selected sequences of DNA, has a sensitivity of a millionfold amplication of a single cell, and thus constitutes a very precise technique for measuring the presence of tumor cells. By combining this technique with genomic hybridization, cadherin,ß and metalloproteinase expression we may then reveal other important factors in the detection, staging, aggressiveness, and treatment of this disease.
Download or read book Report of the Public Schools of Newfoundland Under Methodist Boards written by Newfoundland Superintendent Methodist Schools and published by . This book was released on 1908 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dictyostelium A Tractable Cell and Developmental Model in Biomedical Research written by Robert J. Huber and published by Frontiers Media SA. This book was released on 2022-08-16 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Popular Mechanics written by and published by . This book was released on 2000-05 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: Popular Mechanics inspires, instructs and influences readers to help them master the modern world. Whether it’s practical DIY home-improvement tips, gadgets and digital technology, information on the newest cars or the latest breakthroughs in science -- PM is the ultimate guide to our high-tech lifestyle.
Download or read book Drive written by Sharon Wheatley and published by Greenleaf Book Group. This book was released on 2022-03-01 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Broadway actress and her family go on a cross-country adventure during the COVID-19 shutdown Drive follows the pandemic shut-down journey of Come From Away actress Sharon Wheatley and her family from Broadway's sudden closure to when the curtains finally go back up. Along the way, Wheatley thinks back on the humor and grit of her parents and draws strength from those memories in order to confront the challenges of shepherding her family (and pets) through this unprecedented time, while making hilarious memories along the way. Drive is part travelogue, part Little Miss Sunshine, and all Broadway.
Download or read book Regulation of Biofilm Formation in Two Gamma proteobacteria written by Jana Mueller and published by Stanford University. This book was released on 2010 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: S. oneidensis MR-1 is a dissimilatory metal-reducing bacterium. Under anaerobic conditions S. oneidensis MR-1 attaches to insoluble minerals such as Fe(III) and Mn(IV) oxides and can use these as alternative electron acceptors. Biofilms of S. oneidensis MR- 1 were previously found to be dependent on the mxd gene cluster (mxdABCD). This study identified environmental and genetic factors regulating mxd -dependent and -independent biofilm formation in S. oneidensis MR-1 and a putative function for mxdB in LPS biosynthesis. Physiological experiments determined electron donor starvation as a factor responsible for mxd induction. Tn5 mutagenesis identified the two-component signaling system (TCS) ArcS/ArcA and a putative TCS BarA/UvrY as regulators of mxd expression. ccmC, important for cytochrome c maturation, was found to be involved in biofilm formation in S. oneidensis MR-1. Microarray analysis identified ccmC to play a role in aspects of anaerobic and fermentative metabolism in S. oneidensis MR-1. In conclusion, these data indicate a potential connection between energy metabolism of the cell, mxd and biofilm formation in S. oneidenis MR-1. The natural life cycle of the phylogenetically closest relative V. cholerae A1552 involves the transitioning of cells between different environmental surfaces such as the chitinous shell of Crustaceae and the epithelial layer of the human intestine and vice versa. Biofilms of V. cholerae A1552 vpsA and luxO mutants grown under hydrodynamic flow conditions were found to form pronounced, three-dimensional biofilms that resemble all aspects of wild type biofilms. By genetic experiments, it was shown that in hydrodynamically-grown biofilms this independence of vpsA is due to the expression of rpoS, which is a negative regulator of vpsA expression. Biofilms also underwent substantial metabolically controlled dissolution after 96 h that could be induced by a simple stop of medium flow across the biofilm. These studies indicate that metabolic conditions control the reversible attachment of cells to the biofilm matrix and are key in regulating biofilm cell physiology via rpoS. Furthermore, these results redefine the roles of vps and quorum-sensing in V. cholerae A1552 biofilms.
Download or read book Journal of the National Cancer Institute written by and published by . This book was released on 2009 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bacteria and Fungi from Fish and other Aquatic Animals 2nd Edition written by Nicky B Buller and published by CABI. This book was released on 2014-12-23 with total page 921 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical book provides an updated resource for the identification of bacteria found in animals inhabiting the aquatic environment, illustrated with colour photos. It contains expanded biochemical identification tables to include newly identified pathogenic and saprophytic bacteria, molecular identification tests now available for a greater number of aquatic bacterial pathogens, more information on the pathogenesis and virulence of each organism and new coverage of traditional and molecular identification of fungal pathogens and quality assurance standards for laboratories.
Download or read book Salinity Tolerance in Plants Mechanisms and Regulation of Ion Transport written by Vadim Volkov and published by Frontiers Media SA. This book was released on 2018-01-12 with total page 243 pages. Available in PDF, EPUB and Kindle. Book excerpt: Life presumably arose in the primeval oceans with similar or even greater salinity than the present ocean, so the ancient cells were designed to withstand salinity. However, the immediate ancestors of land plants most likely lived in fresh, or slightly brackish, water. The fresh/brackish water origins might explain why many land plants, including some cereals, can withstand moderate salinity, but only 1 – 2 % of all the higher plant species were able to re-discover their saline origins again and survive at increased salinities close to that of seawater. From a practical side, salinity is among the major threats to agriculture, having been one of the reasons for the demise of the ancient Mesopotamian Sumer civilisation and in the present time causing huge annual economic losses of over 10 billion USD. The effects of salinity on plants include osmotic stress, disruption of membrane ion transport, direct toxicity of high cytoplasmic concentrations of sodium and chloride on cellular processes and induced oxidative stress. Ion transport is the crucial starting point that determines salinity tolerance in plants. Transport via membranes is mediated mostly by the ion channels and transporters, which ensure selective passage of specific ions. The molecular and structural diversity of these ion channels and transporters is amazing. Obtaining the detailed descriptions of distinct ion channels and transporters present in halophytes, marine algae and salt-tolerant fungi and then progressing to the cellular and the whole organism mechanisms, is one of the logical ways to understand high salinity tolerance. Transfer of the genes from halophytes to agricultural crops is a means to increase salt tolerance of the crops. The theoretical scientific approaches involve protein chemistry, structure-function relations of membrane proteins, synthetic biology, systems biology and physiology of stress and ion homeostasis. At the time of compiling this e-book many aspects of ion transport under salinity stress are not yet well understood. The e-book has attracted researchers in ion transport and salinity tolerance. We have combined our efforts to achieve a wider, more detailed understanding of salt tolerance in plants mediated by ion transport, to understand present and future ways to modify and manipulate ion transport and salinity tolerance and also to find natural limits for the modifications.
Download or read book Single Domain Antibodies written by Greg Hussack and published by Springer Nature. This book was released on 2022-02-14 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Single-Domain Antibodies: Methods and Protocols aims to be a useful, practical guide to help researchers further their studies in this field.
Download or read book Experimental Evaluation of the Host Immune Response to Xenogenic Bio derived Collagen Grafts in Rodent Models written by Fang Zheng and published by Leuven University Press. This book was released on 2006-09 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Experimental models and model organisms in cardiac electrophysiology Opportunities and challenges written by Ruben Coronel and published by Frontiers Media SA. This book was released on 2023-10-05 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:
