Download or read book RpsA and Ribosomal Protein S1 written by Justin R. J. Vigar and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Translation initiation rates are fine-tuned by altering interactions between the ribosome and the translation initiation region of an mRNA. In bacteria varying levels of RNA structure attenuate these interactions by masking the ribosome-binding site from the small ribosomal subunit. Recent studies have described diverse strategies for recruiting mRNA to the ribosome, and highlighted the contributions of ribosomal protein S1. Here, I provide evidence that the non-canonical initiation mechanism that governs translation of the rpsA mRNA, encoding ribosomal protein S1, contains a three-dimensional architecture that is required for efficient translation. Furthermore, S1 plays an essential role during the initiation phase of translation by recruiting mRNA to the ribosome-unfolding structured mRNAs, and allowing for correct start codon positioning on the ribosome. Combining crosslinking immunoprecipitation and high-throughput sequencing approaches revealed the extent of S1's involvement in mRNA recruitment, while also highlighting a broader role as a regulator of many RNA classes.
Download or read book The RpsA Gene and Its Product Ribosomal Protein S1 written by Jalyce L. Heller and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The rpsA gene contains a translation initiation region (TIR) which lacks a Shine-Dalgarno element, is highly structured, and which efficiently drives translation in bacteria. The rpsA gene is downregulated by its gene product, ribosomal protein S1. To investigate how the structure of the TIR facilitates function both in translation and downregulation, we employed a combined SAXS, SHAPE, and computational approach. The structure-function relationship reveals that the introduction of single nucleotide substitutions in the TIR alters its structure, corresponding to a modulated translation efficiency in vivo. The activity of the rpsA TIR in a minimal in vitro transcription/translation system was investigated to understand the TIR's requirement for additional factors. We observed that S1 is strictly required for its translation, and that S1 specifically recognizes and binds to the TIR when in excess over ribosomes. The structure-driven mechanism of this TIR may represent a previously overlooked strategy of translation initiation in bacteria.
Download or read book The Role of Ribosomal Protein S1 in Species specific Protein Synthesis written by Mark Wayne Roberts and published by . This book was released on 1987 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Synthesis and Ribosome Structure written by Knud H. Nierhaus and published by John Wiley & Sons. This book was released on 2004-10-15 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knud Nierhaus, who has studied the ribosome for more than 30 years, has assembled here the combined efforts of several scientific disciplines into a uniform picture of the largest enzyme complex found in living cells, finally resolving many decades-old questions in molecular biology. In so doing he considers virtually all aspects of ribosome structure and function -- from the molecular mechanism of different ribosomal ribozyme activities to their selective inhibition by antibiotics, from assembly of the core particle to the regulation of ribosome component synthesis. The result is a premier resource for anyone with an interest in ribosomal protein synthesis, whether in the context of molecular biology, biotechnology, pharmacology or molecular medicine.
Download or read book The Function of Ribosomal Protein S 1 one in Protein Synthesis written by Gerbrand van Dieijen and published by . This book was released on 1977 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ribosomes written by Alexander Spirin and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is based on an advanced course of lectures on ribosome structure and protein biosynthesis that I offer at the Moscow State University. These lectures have been part of a general course on molecular biology for almost three decades, and they have undergone considerable evolution as knowledge has been pro gressing in this field. The progress continues, and readers should be prepared that some facts, statements, and ideas included in the book may be incomplete or out of-date. In any case, this is primarily a textbook, but not a comprehensive review. It provides a background of knowledge and current ideas in the field and gives ex amples of observations and their interpretations. I understand that some interpre tations and generalizations may be tentative or disputable, but I hope that this will stimulate thinking and discussing better than if I left white spots. The book has a prototype: it is my monograph "Ribosome Structure and Pro tein Biosynthesis" published by the Benjamin/Cummings Publishing Company, Menlo Park, California, in 1986. Here I have basically kept the former order of pre sentation ofthe topics and the subdivision into chapters. The contents ofthe chap ters, however, have been significantly revised and supplemented. The newly writ ten chapters on translational control in prokaryotes (Chapter 16) and eukaryotes (Chapter 17) are added.
Download or read book Ribosomal Protein S1 written by Jesper Cairo Westergaard and published by . This book was released on 2006 with total page 33 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of RNA Processing and Decay in Prokaryotes written by and published by Academic Press. This book was released on 2009-03-10 with total page 519 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleic acids are the fundamental building blocks of DNA and RNA and are found in virtually every living cell. Molecular biology is a branch of science that studies the physicochemical properties of molecules in a cell, including nucleic acids, proteins, and enzymes. Increased understanding of nucleic acids and their role in molecular biology will further many of the biological sciences, including genetics, biochemistry, and cell biology. Progress in Nucleic Acid Research and Molecular Biology is intended to bring to light the most recent advances in these overlapping disciplines with a timely compilation of reviews comprising each volume. - This series provides a forum for discussion of new discoveries, approaches, and ideas - Contributions from leading scholars and industry experts - Reference guide for researchers involved in molecular biology and related fields
Download or read book Studies on the Translation of Gengenetic Message written by Joseph Sobura and published by . This book was released on 1975 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mechanisms of Protein Synthesis written by E. Bermek and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains the papers presented at the international symposium on "Molecular Mechanisms in Protein Synthesis" held on September 26-27, 1983 at the Beyaz Ko§k in Emirgan, Bosphorus, Istanbul. The symposium aimed to create a medium for information exchange and discussions regarding the current developments in the area of protein syn thesis. To ensure an informal yet scientifically stimulating and productive atmosphere providing opportunity for relaxed and speculative discussions, the number of presentations was limited to twenty and that of attendants to about sixty. The emphasis in the symposium was laid on structure-function relations in the prokaryotic protein synthesizing systems and on the control mechanisms of eukaryotic protein synthesis, in particular, during chain initia tion. Other issues like evolutionary aspects of protein synthesis, translational components genes and proofreading were covered as well. The manuscripts represent the extended accounts of the oral presenta tions, and it has been aimed with the concluding remarks at the end of the volume to give a summarizing view of the presentations and the discussions.
Download or read book Recent Progress of Biochemical and Biomedical Engineering in Japan II written by Takeshi Kobayashi and published by Springer Science & Business Media. This book was released on 2004-07-21 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: The areas we deal with in biochemical engineering have expanded to include many various organisms and humans. This book has gathered together the information of these expanded areas in biochemical engineering in Japan. These two volumes are composed of 15 chapters on microbial cultivation techniques, metabolic engineering, recombinant protein production by transgenic avian cells to biomedical engineering including tissue engineering and cancer therapy. Hopefully, these volumes will give readers a glimpse of the past and also a view of what may happen in biochemical engineering in Japan.
Download or read book Antibiotics written by Peter Sass and published by Springer Nature. This book was released on 2022-11-29 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, as well as methods for the analysis and detection of microbial responses and adaptation strategies. Antibiotics: Methods and Protocols, Second Edition, guides readers through updated and entirely new chapters on production and design, mode of action, and response and resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antibiotics: Methods and Protocols, Second Edition aims to inspire scientific work in the exciting field of antibiotic research.
Download or read book Bacteria and Bacteriophage written by Edwin J. Atencio and published by Elsevier. This book was released on 2014-05-10 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleotide Sequences 1986/1987, Volume V: Bacteria and Bacteriophage presents data that reflect the information found in GenBank Release 44.0 of August 1986. This book provides information pertinent to the unique international collaboration between two leading nucleotide sequence data libraries, one based in Europe and one in the United States. Organized into two sections, this volume begins with an overview of the sequences, some basic identifying information, and some of the biological annotations. This text then discusses the EMBL Nucleotide Sequence Data Library, an international center of fundamental research with its main focus in the fields of cell biology, molecular structures, instrumentation, and differentiation. This book discusses as well the GenBank database established in 1982 by the National Institute of General Medical Sciences (NIGMS) of the U.S National Institutes of Health (NIH). This book is a valuable resource for molecular biologists and other investigators collecting the large number of reported DNA and RNA sequences and making them available in computer-readable form.
Download or read book Computational Biology and Genome Informatics written by Jason T. L. Wang and published by World Scientific. This book was released on 2003 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains articles written by experts on a wide range of topics that are associated with the analysis and management of biological information at the molecular level. It contains chapters on RNA and protein structure analysis, DNA computing, sequence mapping, genome comparison, gene expression data mining, metabolic network modeling, and phyloinformatics. The important work of some representative researchers in bioinformatics is brought together for the first time in one volume. The topic is treated in depth and is related to, where applicable, other emerging technologies such as data mining and visualization. The goal of the book is to introduce readers to the principle techniques of bioinformatics in the hope that they will build on them to make new discoveries of their own. Contents: Exploring RNA Intermediate Conformations with the Massively Parallel Genetic Algorithm; Introduction to Self-Assembling DNA Nanostructures for Computation and Nanofabrication; Mapping Sequence to Rice FPC; Graph Theoretic Sequence Clustering Algorithms and their Applications to Genome Comparison; The Protein Information Resource for Functional Genomics and Proteomics; High-Grade Ore for Data Mining in 3D Structures; Protein Classification: A Geometric Hashing Approach; Interrelated Clustering: An Approach for Gene Expression Data Analysis; Creating Metabolic Network Models Using Text Mining and Expert Knowledge; Phyloinformatics and Tree Networks. Readership: Molecular biologists who rely on computers and mathematical scientists with interests in biology.
Download or read book Sulfur Amino Acids Advances in Research and Application 2013 Edition written by and published by ScholarlyEditions. This book was released on 2013-06-21 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sulfur Amino Acids—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Cystathionine. The editors have built Sulfur Amino Acids—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Cystathionine in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Sulfur Amino Acids—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Cell Free Translation Systems written by A.S. Spirin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a unique book that describes the most recent achievements in the methodology of protein biosynthesis under cell-free conditions. Various versions of cell-free protein-synthesizing systems and their applications to production of individual proteins on a preparative scale are reviewed. The most recent, advanced methodologies, such as continuous-exchange and continuous-flow cell-free systems and novel effecting batch-format cell-free procedures, are considered. Special attention is drawn to the possibilities of structural (NMR; X-ray) analysis of various gene expression products with the use of a new generation of cell-free systems.
Download or read book Gene Function written by Robert E. Glass and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: My aim in writing Gene Function has been to present an up-to-date picture ofthe molecular biology of Escherichia coli. I have not attempted a chronological description, believing that a mechanistic account is more useful for such a highly developed field. I have divided the book into four parts. Part I is a general introduction to bacterial systems, their genetic material, structure, composition and growth. It has seemed desirable to include herein a brief preview of the remaining text, to introduce the nomenclature and to help place subsequent chapters in perspective. The expression of genetic material and its perturbation through mutation is considered in Part II. Part III discusses how the transfer of prokaryotic genetic material can be mediated by plasmids and bacteriophages. It describes the DNA transactions involved (replication, recombination and repair) and ends with a description of the genetic and biochemical techniques employed in the study of gene organisation. Finally, Part IV considers the control of expression of bacterial, plasmid and phage genes. Key reviews are listed at the end of each chapter.
