Download or read book Rolling Circle Amplification RCA written by Vadim V. Demidov and published by Springer. This book was released on 2016-11-09 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the latest developments in rolling circle amplification (RCA) technology with applications in clinical diagnostic tests and molecular medicine. Topics covered include new enzymes useful in RCA, techniques involving RCA for enhanced signal amplification, novel RCA diagnostics, sensors for expediting RCA detection, and prospective RCA-based therapeutics. This is a valuable book for university professors and students in the field of biomedical engineering and biomolecular pharmacology as well as R&D managers of biotechnology and biopharmaceutical companies. Specifically, this book: Reviews prospective RCA-based therapeutics, including RCA-derived DNA nanoparticles that strongly bind to cancer cells Expands readers’ understanding of sensor systems for expediting detection of RCA products by using probe-tagged magnetic nanobeads Maximizes reader insights into novel RCA diagnostics, such as PNA openers-assisted RCA for detection of single target cells and in situ RCA diagnosis of cancer cells and malignant tissues Presents innovative methods for quasi-exponential enhancement of RCA-generated signals, such as nicking enzyme-assisted cascade RCA and RCA coupled with loop-mediated amplification Advance Praise for Rolling Circle Amplification (RCA): “This book provides a badly needed compendium of innovative RCA methods and applications. It should help further increase the community of scientists that have employed RCA in research and diagnostic programs.”— Charles Cantor, Professor Emeritus of Biomedical Engineering, Boston University Executive Director, Retrotope Inc. (USA) “In this new book Vadim Demidov has assembled an enticing menu of articles that illustrate the evolution of the RCA field, including improved protein parts for building superior DNA nanomachines, enhanced modalities of amplification and detection, diagnostic applications, and even a sampling of potential therapeutic applications. The reader will appreciate that while RCA has come of age, there is no lack of exciting surprises, turns, and twists in the continuing evolution of the technology.”— Paul Lizardi, Professor of Pathology, Yale University School of Medicine (retired) Investigator, University of Granada, Spain, President, PetaOmics, Inc., San Marcos, Texas.
Download or read book Genetic Engineering 1 written by and published by . This book was released on 1981 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Virus Evolution written by Marilyn J. Roossinck and published by Springer Science & Business Media. This book was released on 2008-02-23 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive look at the field of plant virus evolution. It is the first book ever published on the topic. Individual chapters, written by experts in the field, cover plant virus ecology, emerging viruses, plant viruses that integrate into the host genome, population biology, evolutionary mechanisms and appropriate methods for analysis. It covers RNA viruses, DNA viruses, pararetroviruses and viroids, and presents a number of thought-provoking ideas.
Download or read book Photochemistry written by Stefano Crespi and published by Royal Society of Chemistry. This book was released on 2021-09-20 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing critical analysis of emerging and well-established topics, this book is essential reading for anyone wanting to keep up to date with the literature on photochemistry and its applications. Volume 49 combines reviews on the latest advances in photochemical research with specific highlights in the field. The first section includes periodical reports of the recent literature on physical and inorganic aspects, including reviews of the molecules employed as dyes in art, light induced reactions in cryogenic matrices, photobiological systems studied by time-resolved infrared spectroscopy and photophysics, and photochemistry of transition metal complexes. This selection is completed by reviews of the literature on solar photocatalysis for water decontamination and disinfection and for water splitting/hydrogen production. Coverage continues in the second part with highlighted topics, from the use of aromatic carbonyls as photocatalysts and photoinitiators in synthesis, photoinduced and photocatalysed decarboxylation reactions, development of dye-sensitized solar cells, design of luminescent water-soluble systems, and applications of plasmonic nanoparticles. This volume also includes a third section entitled ‘SPR Lectures on Photochemistry’, where leading scientists in photochemistry provide examples to introduce a photochemical topic to academic readers, offering precious assistance to students in this field.
Download or read book DNA Amplification written by Vadim V. Demidov and published by Taylor & Francis. This book was released on 2004 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whereas most books on DNA amplification focus on PCR-based technologies, this volume presents a wider range of methods to amplify DNA with an emphasis on their diverse applications. The book covers both well-established and newly-developed protocols including ligation-based thermocycling approaches, real-time PCR and other new PCR developments, plus several powerful non-PCR isothermal DNA amplification techniques, for example: real-time strand displacement amplification (SDA), rolling-circle amplification (RCA) and multiple-displacement amplification (MDA). An entire section is devoted to a group of enzymes, both natural and engineered, which are employed for DNA amplification and related purposes. In addition, the use of DNA amplification in the detection of non-DNA analytes is presented.
Download or read book DNA Virus Replication written by Alan Cann and published by Oxford University Press, USA. This book was released on 2000 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA viruses have always been the most important model systems for eukaryotic DNA replication. This volume concentrates on the theme of protein-protein interactions in DNA virus replication.
Download or read book Nucleic Acid Detection and Structural Investigations written by Kira Astakhova and published by Humana. This book was released on 2020-11-14 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book volume provides in depth coverage of the nucleic acid field and aims to represent a broad diversity of the methodologies and a wide range of nucleic-acid-related topics within the fields of molecular biology and biomedicine. Chapters are divided in three sections detailing in vitro detection, nanotechnology and imaging, biomedical applications, and big data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nucleic Acid Detection and Structural Investigations: Methods and Protocols aims present aims to present methodologies combine cutting-edge innovation with sound theory and practical applications in life sciences.
Download or read book Advanced Techniques in Diagnostic Microbiology written by Yi-Wei Tang and published by Springer Science & Business Media. This book was released on 2007-01-16 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clinical microbiologists are engaged in the field of diagnostic microbiology to determine whether pathogenic microorganisms are present in clinical specimens collected from patients with suspected infections. If microorganisms are found, these are identified and susceptibility profiles, when indicated, are determined. During the past two decades, technical advances in the field of diagnostic microbiology have made constant and enormous progress in various areas, including bacteriology, mycology, mycobacteriology, parasitology, and virology. The diagnostic capabilities of modern clinical microbiology laboratories have improved rapidly and have expanded greatly due to a technological revolution in molecular aspects of microbiology and immunology. In particular, rapid techniques for nucleic acid amplification and characterization combined with automation and user-friendly software have significantly broadened the diagnostic arsenal for the clinical microbiologist. The conventional diagnostic model for clinical microbiology has been labor-intensive and frequently required days to weeks before test results were available. Moreover, due to the complexity and length of such testing, this service was usually directed at the hospitalized patient population. The physical structure of laboratories, staffing patterns, workflow, and turnaround time all have been influenced profoundly by these technical advances. Such changes will undoubtedly continue and lead the field of diagnostic microbiology inevitably to a truly modern discipline. Advanced Techniques in Diagnostic Microbiology provides a comprehensive and up-to-date description of advanced methods that have evolved for the diagnosis of infectious diseases in the routine clinical microbiology laboratory. The book is divided into two sections. The first techniques section covers the principles and characteristics of techniques ranging from rapid antigen testing, to advanced antibody detection, to in vitro nucleic acid amplification techniques, and to nucleic acid microarray and mass spectrometry. Sufficient space is assigned to cover different nucleic acid amplification formats that are currently being used widely in the diagnostic microbiology field. Within each technique, examples are given regarding its application in the diagnostic field. Commercial product information, if available, is introduced with commentary in each chapter. If several test formats are available for a technique, objective comparisons are given to illustrate the contrasts of their advantages and disadvantages. The second applications section provides practical examples of application of these advanced techniques in several "hot" spots in the diagnostic field. A diverse team of authors presents authoritative and comprehensive information on sequence-based bacterial identification, blood and blood product screening, molecular diagnosis of sexually transmitted diseases, advances in mycobacterial diagnosis, novel and rapid emerging microorganism detection and genotyping, and future directions in the diagnostic microbiology field. We hope our readers like this technique-based approach and your feedback is highly appreciated. We want to thank the authors who devoted their time and efforts to produce their chapters. We also thank the staff at Springer Press, especially Melissa Ramondetta, who initiated the whole project. Finally, we greatly appreciate the constant encouragement of our family members through this long effort. Without their unwavering faith and full support, we would never have had the courage to commence this project.
Download or read book Begomoviruses Occurrence and Management in Asia and Africa written by Sangeeta Saxena and published by Springer. This book was released on 2017-10-14 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Begomoviruses are one of the most interesting plant viruses to study for basic and applied research as they cause huge economic losses to agriculture industries and farmers all over the world. They belong to family Geminiviridae and are emergent plant viral pathogens which cause diseases in various crops in the tropical and subtropical regions. They are transmitted by the whitefly (B. tabaci) and have either one (monopartite DNA-A) or two (bipartite DNA-A and DNA-B) genomic components. DNA-A and DNA-B are of ~2600 - 2800 nucleotides each. A number of serious diseases of cultivated crops of the Fabaceae, Malvaceae, Solanaceae and Cucurbitaceae families are caused by Begomoviruses which are considered as threat to their cultivation in many countries. Accurate diagnosis is important for successful diseases management, since plants infected by Begomovirus do not recover, suffer serious yield losses and act as further sources of inoculum, which is then picked up and spread by their vector whitefly (B. tabaci). Reports of occurrence of new viruses and re-emergence of several known viruses in new niches have become regular event. In such a dynamic system, growth of several crop species relies on an accurate diagnosis, management and better understanding of the biology of the casual virus. This is crucial to evolve appropriate control practices and to prevent the virus infection. Researchers have achieved considerable progress in characterization, detection and management of virus on different crop species in the last decade. This book covers latest information in diagnosis of begomoviruses in the present scenario and explores the new vistas in the field of genomics and proteomics. Chapters in Section 1 illustrates the occurrence, genome organisation, transmission and diagnostics of begomoviruses. It also details the diseases caused by begomoviruses on different crops, detection techniques and management strategies in support of research findings by presentation of data, graphics, figures and tables. Section 2 is a chapterwise collection of occurrence, diversity and status of begomoviruses in Asian Africa counties where the diseases are most prevalent. This book will provide wide opportunity to the readers to have complete information and status of begomovirus in Asia and Africa. This will be useful resource for researchers and extension workers involved in the begomvirus disease diagnosis and molecular biology. Expert detection, accurate diagnosis and timely management play a significant role in keeping plants free from pathogens. In this book expert researchers share their research knowledge and literature which are vital towards the diagnosis of begomoviruses, addressing traditional plant pathology techniques as well as advanced molecular diagnostic approach. The book deals with the economically important crops including fruits, vegetables along with challenges in crop protection against diseases caused by begomovirus. This will be resourceful and handy for researcher, practitioners and also students.
Download or read book Electrochemical DNA Biosensors written by Mehmet Sengun Ozsoz and published by CRC Press. This book was released on 2012-04-23 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the basic electrochemical applications of DNA in various areas, from basic principles to the most recent discoveries. The book comprises theoretical and experimental analysis of various properties of nucleic acids, research methods, and some promising applications. The topics discussed in the book include electrochemical detection of DNA hybridization based on latex/gold nanoparticle and nanotubes; nanomaterial-based electrochemical DNA detection; electrochemical detection of microorganism-based DNA biosensors; gold nanoparticle-based electrochemical DNA biosensors; electrochemical detection of the aptamer-target interaction; nanoparticle-induced catalysis for DNA biosensing; basic terms regarding electrochemical DNA (nucleic acids) biosensors; screen-printed electrodes for electrochemical DNA detection; application of field-effect transistors to label free electrical DNA biosensor arrays; and electrochemical detection of nucleic acids using branched DNA amplifiers.
Download or read book Complete Jethro Burns Mandolin written by Jethro Burns and published by Mel Bay Publications. This book was released on 2015-12-22 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn from the master of bluegrass mandolin - Jethro Burns. More than 70 tunes and two dozen exercises are found in this bluegrass bonanza. The Complete Jethro Burns Mandolin Book and online audio is an incomparable resource of bluegrass mandolin repertoire - all in standard notation and tablature, just the way the maestro played them - with chord symbols for an accompaniment instrument. Presents some of Jethro's finest and most challenging solos, in addition to a special section on Jethro's mandolin techniques. Ample performance notes and performance notes and mandolin lore are provided, along with many photographs of Jethro in the latter half of a career lasting more than half a century. The recording presents 52 of the tunes in the book available online for download
Download or read book Microbiology written by Joan Slonczewski and published by W. W. Norton. This book was released on 2017-07-03 with total page 78 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most current and visually engaging introduction to general microbiology.
Download or read book Directed Evolution Library Creation written by Elizabeth M.J. Gillam and published by Springer. This book was released on 2014-07-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. As well as experimental methods, information on current computational approaches is provided in a user-friendly format that will allow researchers to make informed choices without needing to comprehend the full technical details of each algorithm. Directed evolution has become a fundamental approach for engineering proteins to enhance activity and explore structure-function relationships, and has supported the rapid development of the field of synthetic biology over the last decade. Divided into three convenient sections, topics include point mutagenesis strategies, recombinatorial methods wherein genetic diversity is sourced from multiple parental genes that are combined via either homology-dependent or -independent techniques and a variety of computational methods to guide the design and analysis of mutant libraries. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Directed Evolution Library Creation: Methods and Protocols, Second Edition will serve as a reliable manual for both novice and experienced protein engineers and synthetic biologists and will enable further technical innovation and the exploitation of directed evolution for a deeper understanding of protein design and function.
Download or read book Enzyme Activity in Single Cells written by and published by Academic Press. This book was released on 2019-10-29 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme Activity in Single Cells, Volume 628, the latest release in the Methods of Enzymology series, discusses groundbreaking cellular physiology research that is taking place in the biological sciences. Chapters in this new release cover Spatial and temporal resolution of caspase waves in single Xenopus eggs during apoptosis, Spatial and temporal organization of metabolic complexes in cells, Measuring cellular efflux and biomolecular delivery: synthetic approaches to imaging and engineering cells, Slide-based, single-cell enzyme assays, Single-cell assays using integrated continuous-flow microfluidics, High-throughput screening of single-cell lysates, Microfluidic capture of single cells for drug resistance assays, and much more.
Download or read book DNA Protein Interactions written by Andrew Arthur Travers and published by Springer Science & Business Media. This book was released on 1993-04-30 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: The binding of proteins to DNA and the manipulation of DNA by proteins are crucial aspects of the biological role of DNA in the living cell. This book provides a comprehensive and lucid discussion of the molecular interactions involved.
Download or read book Open Space Microfluidics written by Emmanuel Delamarche and published by John Wiley & Sons. This book was released on 2018-01-18 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Summarizing the latest trends and the current state of this research field, this up-to-date book discusses in detail techniques to perform localized alterations on surfaces with great flexibility, including microfluidic probes, multifunctional nanopipettes and various surface patterning techniques, such as dip pen nanolithography. These techniques are also put in perspective in terms of applications and how they can be transformative of numerous (bio)chemical processes involving surfaces. The editors are from IBM Zurich, the pioneers and pacesetters in the field at the forefront of research in this new and rapidly expanding area.
Download or read book Genotyping written by Stefan J. White and published by Humana. This book was released on 2016-11-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.
