Download or read book Ribosomal RNA protein Interactions in Escherichia Coli microform Binding of Ribosomal Protein S20 to 16 S RRNA and Processing of 5 S RRNA by Ribonuclease E written by Robert Stephen Cormack and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 1994 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: The role of the polypeptide encoded by ams/rne/hmp1, a gene required for RNase E activity, was investigated. Northwestern blotting and UV crosslinking of substrate RNA to crude fractions containing RNase E unambiguously showed that overexpressed Ams/Rne/Hmp1 polypeptide has a high affinity for RNA. Our results demonstrate that the ams/rne/hmp1 gene product intimately associates with RNase E substrate RNA and supports the notion that Ams/Rne/Hmp1 is the nuclease responsible for RNase E activity. In addition, the RNA-binding domain of Ams/Rne/Hmp1 was localized and a putative RNase E-associated protein was identified.
Download or read book Ribosomal RNA protein Interactions in Escherichia Coli written by Robert Stephen Cormack and published by . This book was released on 1993 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein RNA Interactions in the 30S Subunit of the E Coli Ribosome and the Three dimensional Structure of 16S RRNA written by Seth Richard Stern and published by . This book was released on 1988 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Role of Long Range Interactions in Assembly of the Small Subunit of Escherichia Coli Ribosome written by Deepika S. Calidas and published by . This book was released on 2012 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The function of the small subunit (SSU) of the ribosome of Escherichia coli is dependent on dynamic interactions at the intersection of its four domains; namely, the body, platform, head and penultimate stem. The in vitro assembly of each individual domain from its corresponding structural element in 16S ribosomal RNA (rRNA), i.e., the 5', central, 3' major and minor domains and associated ribosomal proteins (r-proteins) has been extensively researched. Less is understood of the long range interactions that occur during assembly as different domains co-assemble, both in vitro and in vivo. Our first approach was to use directed probing from the S8 r-protein as a monitor of SSU assembly. We found that assembly of the neck, a functionally significant region between the head and platform is dependent on assembly of the body. Furthermore, S8 binds two distinct binding sites in 16S rRNA separated by several hundred nucleotides, and the appropriate architecture of the later transcribed region is dependent upon incorporation of r-proteins to the earlier transcribed region. Elements of the body domain, including the 5' terminus do not assume their appropriate conformation except upon assembly of the entire domain. Also, we found that S12 could influence the architecture of the 5' terminus, leading us to examine the role of S12 in 30S subunit assembly, both in vitro and in vivo. S12 possesses a non-canonically structured extension that extends from the solvent surface to the intersubunit surface of the SSU, contacting multiple domains. An almost complete truncation of the extension was unable to support growth, while partial truncations of more than 6 amino acids exhibited growth defects. Truncation of half or all of the extension also resulted in reduced activity of SSUs assembled in vitro. The architecture of ribonucleoprotein complexes assembled with truncated proteins is also altered. The work presented in this thesis elucidates influence of widely separated elements of the SSU on each other during assembly"--Page v.
Download or read book Interaction of 16S and 23S Ribosomal RNA with Functional Ligands Implications for the Mechanism of Protein Synthesis written by Danesh Moazed and published by . This book was released on 1989 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies on the Specific Interaction of Ribosomal Protein S8 from Escherichia Coli with Its Binding Site in 16S RRNA written by Irina V. Alimova and published by . This book was released on 2001 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein RNA Interactions in the Escherichia Coli 30S Ribosomal Subunit written by Jean E. Schwarzbauer and published by . This book was released on 1980 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies of the Ribosomal Binding Sites of and Cooperativity of Binding of Protein Synthesis Initiation Factors of Escherichia Coli written by Steven Robert Langberg and published by . This book was released on 1978 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Use of Chemical Crosslinking in Structural Studies of Escherichia Coli Ribosomal RNA written by Seán Turner and published by . This book was released on 1985 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA protein Interactions written by Kiyoshi Nagai and published by Oxford University Press, USA. This book was released on 1994 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.
Download or read book Chemical Modification of the Messenger RNA Binding Site on the 30S Ribosomal Subunits of Escherichia Coli written by Chu Chang and published by . This book was released on 1977 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Ribosomal Protein and Ribosomal RNA Synthesis in Escherichia Coli written by Sue Jinks Robertson and published by . This book was released on 1983 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Several Contributions to the Understanding of Ribosome Structure written by Eleanor Spicer Ward and published by . This book was released on 1975 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Probing Studies of the Surface and Subunit Interface of Escherichia Coli Ribosomes written by Chuck Merryman and published by . This book was released on 2000 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Function of Escherichia Coli Ribosomal Protein S1 in Initiation of Translation written by Wayne E. Taylor and published by . This book was released on 1977 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Procaryotic protein RNA interactions Ribonuclease studies of Escherichia coli and Bacillus stearothermophilus 5S RNA s and 5S RNA protein complexes written by Stephen R. Douthwaite and published by . This book was released on 1981 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book RNA protein Cross linking Within the Intact Escherichia Coli Ribosome Utilizing Ethylene Glycol bis 3 2 ketobutyraldehyde Ether a Reversible Bifunctional Reagent written by Linda Anne Brewer and published by . This book was released on 1981 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:
