Download or read book Restriction Endonucleases and Methylases written by Jack G. Chirikjian and published by Elsevier Publishing Company. This book was released on 1987 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Restriction Endonucleases and Methylases, the fifth volume in the series, Gene Amplification and Analysis, covers important new research advances in molecular biology brought about by the use of restriction endonucleases and methylases. Contributions by authorities in the field focus on research studies that examine both the structure and catalytic properties of this important group of DNA enzymes. This volume also serves as a useful reference for reaction conditions and the general application of restriction endonucleases as reagents for molecular biology. Restriction Endonucleases and Methylases is invaluable reading for those who wish to better understand the restriction enzymes utilized in various areas of research in molecular biology.
Download or read book Restriction Endonucleases written by Alfred Pingoud and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt: Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
Download or read book DNA Repair and Mutagenesis written by Errol C. Friedberg and published by American Society for Microbiology Press. This book was released on 2005-11-22 with total page 2587 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential resource for all scientists researching cellular responses to DNA damage. • Introduces important new material reflective of the major changes and developments that have occurred in the field over the last decade. • Discussed the field within a strong historical framework, and all aspects of biological responses to DNA damage are detailed. • Provides information on covering sources and consequences of DNA damage; correcting altered bases in DNA: DNA repair; DNA damage tolerance and mutagenesis; regulatory responses to DNA damage in eukaryotes; and disease states associated with defective biological responses to DNA damage.
Download or read book Techniques in Molecular Biology written by J.M. Walker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
Download or read book Mobile DNA II written by Nancy L. Craig and published by Amer Society for Microbiology. This book was released on 2002 with total page 1204 pages. Available in PDF, EPUB and Kindle. Book excerpt: An extension of the original volume, reflecting the latest advances in understanding these elements. This title is published by the American Society for Microbiology Press and distributed by Taylor and Francis in rest of world territories.
Download or read book Bacterial Genomes written by F.J. de Bruijn and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 786 pages. Available in PDF, EPUB and Kindle. Book excerpt: A wide range of microbiologists, molecular biologists, and molecular evolutionary biologists will find this new volume of singular interest. It summarizes the present knowledge about the structure and stability of microbial genomes, and reviews the techniques used to analyze and fingerprint them. Maps of approximately thirty important microbes, along with articles on the construction and relevant features of the maps are included. The volume is not intended as a complete compendium of all information on microbial genomes, but rather focuses on approaches, methods and good examples of the analysis of small genomes.
Download or read book DNA Methylation written by A. Razin and published by Springer. This book was released on 2011-11-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past few decades we have witnessed an era of remarkable growth in the field of molecular biology. In 1950 very little was known of the chemical constitution of biological systems, the manner in which information was trans mitted from one organism to another, or the extent to which the chemical basis of life is unified. The picture today is dramatically different. We have an almost bewildering variety of information detailing many different aspects of life at the molecular level. These great advances have brought with them some breath-taking insights into the molecular mechanisms used by nature for rep licating, distributing and modifying biological information. We have learned a great deal about the chemical and physical nature of the macromolecular nucleic acids and proteins, and the manner in which carbohydrates, lipids and smaller molecules work together to provide the molecular setting of living sys tems. It might be said that these few decades have replaced a near vacuum of information with a very large surplus. It is in the context of this flood of information that this series of monographs on molecular biology has been organized. The idea is to bring together in one place, between the covers of one book, a concise assessment of the state of the subject in a well-defined field. This will enable the reader to get a sense of historical perspective-what is known about the field today-and a description of the frontiers of research where our knowledge is increasing steadily.
Download or read book Enzymology Primer for Recombinant DNA Technology written by Hyone-Myong Eun and published by Elsevier. This book was released on 1996-06-03 with total page 727 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. - Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology - Presents information in an easily accessible format to serve as a quick reference source - Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques
Download or read book Restriction Enzymes written by Wil A. M. Loenen and published by . This book was released on 2019 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Restriction enzymes cleave DNA at specific recognition sites and have many uses in molecular biology, genetics, and biotechnology. More than 4000 restriction enzymes are known today, of which more than 621 are commercially available, justifying their description by Nobel Prize winner Richard Roberts as "the workhorses of molecular biology." This book by Wil Loenen is the first full-length history of these invaluable tools, from their recognition in the 1950s to the flowering of their development in the 1970s and 1980s to their ubiquitous availability today. Loenen has worked with restriction enzymes throughout her career as a research scientist, during which she came to know many of the leaders in this field personally and professionally. She is the author of several authoritative and widely appreciated reviews of the enzymes' biology. Her book was written with the close assistance of several of the field's pioneers, including Rich Roberts, Stuart Linn, Tom Bickle, Steve Halford, and the late Joe Bertani. The seed for the book was sown at a retirement party for Noreen Murray, to whom the book is dedicated, and its roots lie in a remarkable 2013 conference at Cold Spring Harbor Laboratory that celebrated the people and events that were vital to the field's development. Funding for the book was made possible by the Genentech Center for the History of Molecular Biology and Biotechnology at Cold Spring Harbor Laboratory.
Download or read book Bacterial Integrative Mobile Genetic Elements written by Adam P. Roberts and published by CRC Press. This book was released on 2013-01-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: As our understanding of mobile genetic elements continues to grow we are gaining a deeper appreciation of their importance in shaping the bacterial genome and in the properties they confer to their bacterial hosts. These include, but are by no means limited to, resistance to antibiotics, and heavy metals, toxin production and increased virulence, production of antibiotics and the ability to utilize a diverse range of metabolic substrates. We are also gaining an understanding of diversity of these elements and their interactions with each other; a property which continually complicates any attempt to classify them. We are learning more about the molecular mechanisms by which they translocate to new genomic sites both within genomes and between different bacteria. This book provides a timely, state of the art update on the properties of an important selection of different bacterial integrative mobile genetic elements and the myriad of different ways in which they move and influence the biology of the host bacterium. The chapters are all written by authors who have undertaken pioneering work in their respective fields, making this book vital reading for all who are interested in the biology of bacteria and the mobile elements they carry.
Download or read book DNA Methylation written by J. Jost and published by Birkhäuser. This book was released on 2013-11-11 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: The occurrence of 5-methylcytosine in DNA was first described in 1948 by Hotchkiss (see first chapter). Recognition of its possible physiologi cal role in eucaryotes was first suggested in 1964 by Srinivasan and Borek (see first chapter). Since then work in a great many laboratories has established both the ubiquity of 5-methylcytosine and the catholicity of its possible regulatory function. The explosive increase in the number of publications dealing with DNA methylation attests to its importance and makes it impossible to write a comprehensive coverage of the literature within the scope of a general review. Since the publication of the 3 most recent books dealing with the subject (DNA methylation by Razin A. , Cedar H. and Riggs A. D. , 1984 Springer Verlag; Molecular Biology of DNA methylation by Adams R. L. P. and Burdon R. H. , 1985 Springer Verlag; Nucleic Acids Methylation, UCLA Symposium suppl. 128, 1989) considerable progress both in the techniques and results has been made in the field of DNA methylation. Thus we asked several authors to write chapters dealing with aspects of DNA methyla tion in which they are experts. This book should be most useful for students, teachers as well as researchers in the field of differentiation and gene regulation. We are most grateful to all our colleagues who were willing to spend much time and effort on the publication of this book. We also want to express our gratitude to Yan Chim Jost for her help in preparing this book.
Download or read book Escherichia Coli and Salmonella written by Frederick C. Neidhardt and published by . This book was released on 1996 with total page 2822 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the long–awaited second edition of an invaluable classic! Escherichia coli occupies a central role in contemporary molecular biology. It is the unicellular organism about which most is known – all molecular and cellular biologists will want a copy of this book. In 154 chapters, 250 expert authors and editors present the state of the art. Completely rewritten and restructured, the second edition offers a whole new approach to the subject.
Download or read book Kinetics for the Life Sciences written by H. Gutfreund and published by Cambridge University Press. This book was released on 1995-09-14 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of the book is to introduce the reader to the kinetic analysis of a wide range of biological processes at the molecular level. It is intended to show that the same approach can be used to resolve the number of steps in enzyme reactions, muscle contraction, visual perception and ligand binding receptors that trigger other physiological processes. Attention is also given to methods for characterizing these steps in chemical terms. Although the treatment is mainly theoretical, a wide range of examples and experimental techniques are also introduced and an historical approach is used to demonstrate the development of the theory and experimental techniques of kinetic analysis in biology.
Download or read book Chromosomal Aberrations written by Günter Obe and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromosomale Mutationen sind eine der m|glichen Ursachen f}r Ver{nderungen der Erbinformation. Neben grunds{tzlichen As- pekten, wie Reparaturmechanismen der Zelle oder Ursachen von Chromosomenver{nderungen, werden angewandte Aspekte, z.B. Chromosomen als Testindikatoren der Toxizit{t, behandelt.
Download or read book Genetics for Surgeons written by Patrick John Morrison and published by Remedica. This book was released on 2005 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: Morrison (human genetics, University of Ulster, UK) and Spence (biomedical science, University of Ulster, UK) offer an accessible reference on the genetic disorders that surgeons can expect to meet in general surgical practice. Written in non-technical language, with a glossary, list of abbreviations, and color and b&w photos and medical images, the book supplies an introduction to the nomenclature and technology of molecular biology, and will be a useful starting point for those who wish to extend their knowledge. Annotation :2005 Book News, Inc., Portland, OR (booknews.com).
Download or read book DNA Methylation Protocols written by Jörg Tost and published by Humana Press. This book was released on 2018-08-30 with total page 704 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition volume expands on the previous editions by providing a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis, along with precise quantification of DNA methylation levels on single CpG positions. The chapters in this book are divided into 7 parts: an introduction to the field along with tips on study design and data analysis; global DNA methylation levels; genome-wide DNA methylation analysis; highly multiplexed target regions; locus-specific DNA methylation analysis; DNA methylation analysis of specific biological samples; and hydroxymethylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, DNA Methylation Protocols, Third Edition is a valuable resource for postdoctoral investigators and research scientists who work with different aspects of genetics, and cellular and molecular biology, as well as clinicians who are involved in diagnostics or treatment of diseases with epigenetic components.
Download or read book Epigenetics Methods written by Trygve O Tollefsbol and published by Academic Press. This book was released on 2020-07-08 with total page 740 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the field of epigenetics has grown significantly, driving new understanding of human developmental processes and disease expression, as well as advances in diagnostics and therapeutics. As the field of epigenetics continues to grow, methods and technologies have multiplied, resulting in a wide range of approaches and tools researchers might employ. Epigenetics Methods offers comprehensive instruction in methods, protocols, and experimental approaches applied in field of epigenetics. Here, across thirty-five chapters, specialists offer step-by-step overviews of methods used to study various epigenetic mechanisms, as employed in basic and translational research. Leading the reader from fundamental to more advanced methods, the book begins with thorough instruction in DNA methylation techniques and gene or locus-specific methylation analyses, followed by histone modification methods, chromatin evaluation, enzyme analyses of histone methylation, and studies of non-coding RNAs as epigenetic modulators. Recently developed techniques and technologies discussed include single-cell epigenomics, epigenetic editing, computational epigenetics, systems biology epigenetic methods, and forensic epigenetic approaches. Epigenetics methods currently in-development, and their implication for future research, are also considered in-depth. In addition, as with the wider life sciences, reproducibility across experiments, labs, and subdisciplines is a growing issue for epigenetics researchers. This volume provides consensus-driven methods instruction and overviews. Tollefsbol and contributing authors survey the range of existing methods; identify best practices, common themes, and challenges; and bring unity of approach to a diverse and ever-evolving field. - Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application - Integrates technology and translation with fundamental chapters on epigenetics methods, as well as chapters on more novel and advanced epigenetics methods - Written at verbal and technical levels that can be understood by scientists and students alike - Includes chapters on state-of-the-art techniques such as single-cell epigenomics, use of CRISPR/Cas9 for epigenetic editing, and epigenetics methods applied to forensics
