Download or read book Research Methods in Neurochemistry written by Neville Marks and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: On picking up this first volume of a new series of books the reader may ask the two questions: (a) why research methods? and (b) why in neurochem istry? The answers to these questions are easy - they more than justify the volumes to come and show the strong need for their existence. It is customary to think of methods as a necessary but unexciting means to an end - to relegate advances in methodology to a minor role in the creative, original portion of advances in science. This is not the case; the pace-setting function of methodology is well illustrated in most areas of neurobiology. To formulate our questions to Nature (which is the essence of experimental design), methodology is needed; to get answers to our ques tions we have to devise yet new methods. The chapters of the present volume fully illustrate how the development of a new method can cut a new path how it can open new fields, just as the microscope founded histology. Heter ogeneity of structures presents a formidable challenge for methodology in the nervous system, yet methods for separating the structures are essential if we ever want to decipher the enigma of functional contribution of the ele ments to the whole. The problem is not only physical separation-clearly methods are essential to study complex structures in situ.
Download or read book Research Methods in Neurochemistry Vol 1 written by and published by . This book was released on 1972 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Methods in Neurochemistry written by Neville Marks and published by Springer. This book was released on 1995-12-31 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: On picking up this first volume of a new series of books the reader may ask the two questions: (a) why research methods? and (b) why in neurochem istry? The answers to these questions are easy - they more than justify the volumes to come and show the strong need for their existence. It is customary to think of methods as a necessary but unexciting means to an end - to relegate advances in methodology to a minor role in the creative, original portion of advances in science. This is not the case; the pace-setting function of methodology is well illustrated in most areas of neurobiology. To formulate our questions to Nature (which is the essence of experimental design), methodology is needed; to get answers to our ques tions we have to devise yet new methods. The chapters of the present volume fully illustrate how the development of a new method can cut a new path how it can open new fields, just as the microscope founded histology. Heter ogeneity of structures presents a formidable challenge for methodology in the nervous system, yet methods for separating the structures are essential if we ever want to decipher the enigma of functional contribution of the ele ments to the whole. The problem is not only physical separation-clearly methods are essential to study complex structures in situ.
Download or read book Research Methods in Neurochemistry written by Neville Marks and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Methods in Neurochemistry written by Neville Marks and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Section I Ultrastructure and Fragmentation of Neural Tissue.- 1 Bulk Separation of Neuronal Cell Bodies and Glial Cells in the Absence of Added Digestive Enzymes.- I. Introduction.- II. Bulk Isolation Procedures Requiring No Added Digestive Enzyme(s).- A. The Procedure Developed in the Authors' Laboratory.- B. The Procedure of Nagata et al. (1971).- C. The Procedure of Iqbal and Tellez-Nagel (1972).- D. The Procedure of Jones et al. (1971).- III. General Procedural Comments.- IV. Cell Yield and Biochemical Characterization.- V. Applications in Cellular Neurochemistry.- A. Centrifugal Fractiona.
Download or read book Research Methods in Neurochemistry written by Neville Marks and published by Springer Science & Business Media. This book was released on 2013-11-27 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fifth volume of Research Methods in Neurochemistry represents a milestone in that it marks almost a decade since the inception of the series. Over these ten years there has been an almost exponential growth in neuro chemistry accompanied by numerous technical developments. This is the justification for our series; inevitably we have only been able to cover a frac tion of the methodological innovations of the last decade, but we have tried as much as possible to create a balance between the different approaches and philosophies in the study of the chemical basis of brain function. Thus our original format of grouping chapters under various headings-for instance, studies in intact tissues as distinct from studies describing constituents and isolated enzymes-appears to be justified. Studies on whole animals or tissues retaining cellular organization are vital in providing insights into the neurochemical mechanism underlying functional processes; at the same time, the eventual understanding of function can only be attained on the basis of knowledge of the molecular architecture of the tissue. In the present volume, Oldendorfs chapter on the transport of radiolabeled metabolites across the blood-brain barrier illustrates one side of this equation, whereas Poduslo's chapter on the separation of oligo dendroglia! cells provides new information on the role of these cells in myelogenesis and the distinctive chemical composition of glia as compared to neurons.
Download or read book Research Methods in Neurochemistry written by Neville Marks and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fourth volume of Research Methods in Neurochemistry includes chapters on different aspects of topics touched on in previous volumes and develops a number of new themes as well. The bias, though not entirely intended, is directed toward studies of macromolecules, both at the meta bolic level in relation to protein synthesis and at the structural level in rela tion to specific proteins and lipids. The new departures concern subjects in Section I with marked applied bias: biochemical studies of nervous system tumors and of the cerebrospinal fluid, both of which we hope will be of value to clinical as well as basic scientists. Biogenic amines and the enzymes involved in their metabolism figure again in Section II, where the powerful tool of mass spectrometry receives further treatment in relation to the analysis of dansyl derivatives of trace amines in the brain. Once again we remain grateful to the individual authors, both for their contributions and patience, and to Plenum Press for their continued interest and cooperation. Thanks are also due to colleagues and friends for their comments and criticisms on the series as a whole; suggestions for future volumes will always be welcome and should be sent to one of the editors. Richard Rodnight, London Neville Marks, New York March 1978 ix Contents Section I PROPERTIES OF INTACT NEURAL TISSUES Chapter 1 Biochemical Study of Tumors of the Nervous System. . . . . . . . . . . . . . . . 3 Norman Allen I. Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II. Human Brain Tumors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 A. Autopsy Specimens. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Download or read book Research Methods in Neurochemistry written by Neville Marks and published by . This book was released on 2014-09-01 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Methods in Neurochemistry written by Neville Marks and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the continued rapid expansion of neurochemical research, there has been no shortage of new developments in methodology for this third volume of Research Methods in Neurochemistry. As in previous volumes we have again tried to provide some balance in the subjects represented. The wisdom of this policy may be questioned since it can lead to delay in publica tion, but there are many approaches to the chemical study of the nervous system and a methods book needs to stand on its own as well as be part of a series. In one respect, however, the present volume departs from this policy, in that we have included two chapters on micromethods for analyzing amines and amino acids, both giving special emphasis to dansylation techniques. These chapters are complementary and we feel justified in publishing them in one volume in view of the importance of such micromethods for the study of neural systems. At the other end of the scale, particular attention may be drawn to the chapter by D. D. Gilboe and colleagues describing their remarkable procedures for studying metabolism in the isolated canine brain. We were fortunate also in persuading S. S. Oja to extend the general prin ciples of transport systems he described in Volume 2 to amino acids in brain slices. In addition, there are the usual chapters on components of neural tissues, which once again we have found convenient to divide into enzymes, macromolecules, and other constituents.
Download or read book Research Methods in Neurochemistry Vol 5 written by and published by . This book was released on 1981 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Methods in Neurochemistry V 6 written by Neville Marks and published by . This book was released on 1985 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Methods in Neurochemistry written by Neville Marks and published by Springer. This book was released on 1995-12-31 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: On picking up this first volume of a new series of books the reader may ask the two questions: (a) why research methods? and (b) why in neurochem istry? The answers to these questions are easy - they more than justify the volumes to come and show the strong need for their existence. It is customary to think of methods as a necessary but unexciting means to an end - to relegate advances in methodology to a minor role in the creative, original portion of advances in science. This is not the case; the pace-setting function of methodology is well illustrated in most areas of neurobiology. To formulate our questions to Nature (which is the essence of experimental design), methodology is needed; to get answers to our ques tions we have to devise yet new methods. The chapters of the present volume fully illustrate how the development of a new method can cut a new path how it can open new fields, just as the microscope founded histology. Heter ogeneity of structures presents a formidable challenge for methodology in the nervous system, yet methods for separating the structures are essential if we ever want to decipher the enigma of functional contribution of the ele ments to the whole. The problem is not only physical separation-clearly methods are essential to study complex structures in situ.
Download or read book Guide to Research Techniques in Neuroscience written by Matt Carter and published by Academic Press. This book was released on 2022-03-26 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step
Download or read book Chemical and Cellular Architecture written by Abel Lajtha and published by Springer Science & Business Media. This book was released on 2013-04-18 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: After the completion of the first edition of this series, this editor thought that a new edition would not be warranted in less than IS, perhaps 20, years, but it seems that we live in a time in which rapid changes are the norm and findings in a field such as neurochemistry develop exponentially. The task of a future editor attempting to get a comprehensive neurochemical handbook for the year 2000 would be even less enviable, but by then information processing may be very different. The approach, the design, and the areas covered by each volume and each chapter are necessarily arbitrary, and it is likely that other editors or authors would have approached the coverage or the organization in a different manner. It is hoped, however, that readers will find the series helpful for beginning or for continuing work. There may be some overlap among the various chapters, but insisting on single coverage of an area would at times have restricted treatment to only one point of view and might have truncated and hurt the logical flow of some of the chapters.
Download or read book RESEARCH METHODS IN NEUROCHEMISTRY 1972 written by ED MARKS and published by . This book was released on with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Neurobiology written by Robert Lahue and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid advances in knowledge have led to an increasing interest in neuro biology over the last several years. These advances have been made possible, at least in part, by the use of increasingly sophisticated methodology. Furthermore, research in the most rapidly advancing areas is essentially multidisciplinary and is characterized by contributions from many investi gators employing a variety of techniques. While a grasp of fundamental neurobiological concepts is an obvious prerequisite for those who wish to follow or participate in this field, critical awareness and evaluation of neurobiological research also requires an understanding of sophisticated methodologies. The objective of Methods in Neurobiology is the development of such critical abilities. The reader is exposed to the basic concepts, principles, and instrumentation of key methodologies, and the application of each meth odology is placed in the special context of neurobiological research. The reader will gain familiarity with the terminology and procedures of each method and the ability to evaluate results in light of the particular features of neurobiological preparations and applications.
Download or read book Handbook of Neurochemistry written by Abel Lajtha and published by Springer Science & Business Media. This book was released on 2013-11-09 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt:
