Download or read book Regulation of the Morphogenesis Checkpoint in the Budding Yeast Saccharomyces Cerevisiae written by Lee Szkotnicki and published by . This book was released on 2008 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: In response to cytoskeletal perturbations Saccharomyces cerevisiae cells trigger a morphogenesis checkpoint resulting in a G2 arrest. The arrest is due to the Swe1p kinase, which phosphorylates and inhibits the CDK Cdc28p. Dephosphorylation of Cdc28p is catalyzed by the phosphatase Mih1p, and allows for cell cycle progression. Recent work on regulation of the kinase Swe1p has shown a complex pathway of localization specific degradation. Several of the components involved in Swe1p degradation appear to have conserved roles in degradation of the Wee1 family kinases in multicellular systems. Despite this little is known about the steps leading up to the initiation of Swe1p degradation. In this study we have challenged the model that activation of the Swe1p negative regulator, Hsl1p, results from relief of autoinhibition due to septin interaction. Rather, we conclude Hsl1p is subject to phosphorylation of threonine 273, promoted by the septin-associated kinase Elm1p. We identified elm1 mutants in a screen for defects in Swe1p degradation. Additionaly regulation of the morphogenesis checkpoint appears to be acting though regulation of the phophatase Mih1p. In other eukaryotes, Mih1p homologs (Cdc25-family phosphatases) are thought to be regulated by other checkpoints through control of their phosphorylation. We have shown that Mih1p is phosphorylated in response to morphogenesis checkpoint stress. We have also identified a NLS in Mih1p, mutation of which greatly reduces its in vivo activity. These data combined with data from a competing lab suggest that phosphorylation of Mih1p is indeed regulated. We have also investigated the checkpoint response of budded yeast cells. Specifically we have shown that environmental stresses that depolarize the actin cytoskeleton result in a Swe1p mediated G2 delay.
Download or read book Regulation of Budding Yeast Morphogenesis by the Elm1 Kinase written by Wei Wei and published by . This book was released on 2003 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification of Novel Pathways that Regulate the Morphogenesis Checkpoint of Saccharomyces Cerevisiae written by Aron Richard Marquitz and published by . This book was released on 2005 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell cycle checkpoint control in budding yeast saccharomyces cerevisiae PHD written by Gretchen Louise Kiser and published by . This book was released on 1995 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Regulation of the Exit from Mitosis in Saccharomyces Cerevisiae written by Lena H. Hwang and published by . This book was released on 1998 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Analysis of Mitotic Checkpoint Controls in the Yeast Saccharomyces Cerevisiae written by Penny Alice Tavormina and published by . This book was released on 1998 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Transcriptional Regulation of Meiosis and Spore Morphogenesis in Saccharomyces Cerevisiae written by Shelley Chu and published by . This book was released on 1998 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Characterization of the Abscission Pathway and of Its Regulation by the NoCut Checkpoint in the Budding Yeast Saccharomyces Cerevisiae written by Harald Michael Rauter and published by . This book was released on 2009 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Cycle and Checkpoint Regulation of the Budding Yeast S phase Promoting Factor Dbf4p written by and published by . This book was released on 1999 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Sensing Cell Shape written by John M. Crutchley and published by . This book was released on 2008 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: The morphogenesis checkpoint in the budding yeast Saccharomyces cerevisiae monitors bud emergence. If environmental perturbations delay budding, the cells arrest in G2 until they are able to form a bud. The morphogenesis checkpoint is mediated by Swe1p, the budding yeast homolog of Wee1, which phosphorylates and inhibits Cdc28p, the CDK that drives cell division. In unstressed cells, Swe1p is degraded, a process that is dependent on the kinase Hsl1p. Hsl1p localizes to the septins, which form a collar-like structure at the bud neck. Hsl1p binds to Hsl7p, an adapter protein that recruits Swe1p to the neck. When budding is perturbed, Hsl1p is inactive, thus allowing Swe1p to accumulate and inhibit Cdc28p. Bud formation activates Hsl1p, enabling it to recruit negative regulators of Swe1p to the bud neck, where Swe1p is marked for degradation. Interestingly, our lab has shown that Hsl1p is regulated by cell shape. Here, we reexamine a model that suggests Hsl1p is regulated by an autoinhibitory domain and discover that the model cannot be supported by our data. In addition, we present evidence suggesting that Hsl1p is regulated by an upstream kinase, Elm1p, that phosphorylates Hsl1p within the activation loop. Finally, we identify functional and regulatory regions within Hsl1p. We identify regions that participate in septin binding and Hsl7p binding. But our most interesting discovery is a regulatory region that makes Hsl1p responsive to bud emergence.
Download or read book Multiple Mechanisms Prevent Re replication in Budding Yeast Saccharomyces Cerevisiae written by Van Q. Nguyen and published by . This book was released on 2003 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Stress Activated Protein Kinases written by Francesc Posas and published by Springer Science & Business Media. This book was released on 2008-01-24 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book leading researchers in the field discuss the state-of-the-art of many aspects of SAPK signaling in various systems from yeast to mammals. These include various chapters on regulatory mechanisms as well as the contribution of the SAPK signaling pathways to processes such as gene expression, metabolism, cell cycle regulation, immune responses and tumorigenesis. Written by international experts, the book will appeal to cell biologists and biochemists.
Download or read book Regulation of Processing Body Formation in the Budding Yeast Saccharomyces Cerevisiae written by Julie Weidner and published by . This book was released on 2014 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Cytokinesis in the Budding Yeast Saccharomyces Cerevisiae written by Kay Joanne Simmonite and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Systematic Analysis of Cell Size Control in the Budding Yeast Saccharomyces Cerevisiae written by Michael Alexander Cook and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Cycle Regulation written by Philipp Kaldis and published by Results and Problems in Cell Differentiation. This book was released on 2006-06-26 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a state-of-the-art summary of the latest achievements in cell cycle control research with an outlook on the effect of these findings on cancer research. The chapters are written by internationally leading experts in the field. They provide an updated view on how the cell cycle is regulated in vivo, and about the involvement of cell cycle regulators in cancer.
Download or read book Studies of the Cell Cycle Regulation of Checkpoint Activation and DNA Double Strand Break Repair in Saccharomyces Cerevisiae written by Jacqueline Barlow and published by . This book was released on 2008 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:
