Download or read book Regulation of Cellulase Genes in Thermomonospora Fusca written by Ershen Lin and published by . This book was released on 1987 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of Cellulase Synthesis in Thermomonospora Fusca and Cloning Sequencing and Expression of an Alkaline Serine Protease Gene in Thermomonospora Fusca written by Guifang Lao and published by . This book was released on 1996 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Thermostable Cellulase from a Thermomonospora Gene written by and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity.

Download or read book Studies of the Genetic Regulation of the Thermomonospora Cellulase Complex written by and published by . This book was released on 1992 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goals of this project are to determine the molecular mechanisms regulating cellulose synthesis in the soil bacterium Thermomonosporafusca and to determine the molecular mechanism by which T.fusca cellulases degrade crystalline cellulose. We have determined a structure for the T.fusca E2 catalytic subunit (E230 by x-ray crystallography. This structure is quite similar to that of T.reesei CBHU but there are a number of differences. One is that the E2 active site is in a cleft while that of CBHII is in a tunnel. This is an expected result since E2 is an endocellulase. Large amounts of homogenous E5 catalytic subunit have been prepared and attempts to crystallize it are underway. Crystals of E2-30 were soaked in cellobiose and modified crystals detracted well, however difference Fourier analysis showed many changes, so that we could not localize cellobiose in the 3-D structure of E2-30. This implies that binding of cellobiose causes a significant change in the structure of E2-30. The stereochemistry of the cleavage catalyzed by E{sub l}, E2 and E5 was determined in collaboration with Dr. Stephen Withers and E1 and 2 inverted the glycoside linkage while E5 does not. The entire E{sub l} and E4 genes have been induced into Streptomyces lividans where they are expressed at a high level and the E{sub l} and E4 are completely secreted into the medium. Studies on the synergism between the exocellulase E3 and the endocellulases E2 or E5 show that both exo and endocellulase activities are stimulated when they are assayed together.

Download or read book Studies of the Genetic Regulation of the Thermomonospora Cellulase Complex Progress Report June 1 1990 January 10 1992 written by and published by . This book was released on 1992 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goals of this project are to determine the molecular mechanisms regulating cellulose synthesis in the soil bacterium Thermomonosporafusca and to determine the molecular mechanism by which T.fusca cellulases degrade crystalline cellulose. We have determined a structure for the T.fusca E2 catalytic subunit (E230 by x-ray crystallography. This structure is quite similar to that of T.reesei CBHU but there are a number of differences. One is that the E2 active site is in a cleft while that of CBHII is in a tunnel. This is an expected result since E2 is an endocellulase. Large amounts of homogenous E5 catalytic subunit have been prepared and attempts to crystallize it are underway. Crystals of E2-30 were soaked in cellobiose and modified crystals detracted well, however difference Fourier analysis showed many changes, so that we could not localize cellobiose in the 3-D structure of E2-30. This implies that binding of cellobiose causes a significant change in the structure of E2-30. The stereochemistry of the cleavage catalyzed by E{sub l}, E2 and E5 was determined in collaboration with Dr. Stephen Withers and E1 and 2 inverted the glycoside linkage while E5 does not. The entire E{sub l} and E4 genes have been induced into Streptomyces lividans where they are expressed at a high level and the E{sub l} and E4 are completely secreted into the medium. Studies on the synergism between the exocellulase E3 and the endocellulases E2 or E5 show that both exo and endocellulase activities are stimulated when they are assayed together.

Download or read book Isolation and Characterization of Thermomonospora Fusca Cellulase E2 Mutants written by Chris Kroupis and published by . This book was released on 1991 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Growth Parameters and Cellulase Regulation in Thermomonospora Fusca 190Th written by Richard E. Wise and published by . This book was released on 1988 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization and Genetic Analysis of the Cellulolytic Microorganism Thermobifida Fusca written by Yu Deng and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Currently, one of the hurdles hindering efficient production of cellulosic biofuel is the recalcitrant nature of cellulose to hydrolysis. A wide variety of cellulase enzymes are found natively in microorganisms that can potentially be used to effectively hydrolyze cellulose to fermentable sugars. Thermobifida fusca is a high G-C content, thermophilic, gram-positive soil actinobacterium with high cellulolytic activity. The phenomenological and mechanistic parameters affecting cellulase activity were studied in T. fusca and two mechanisms have been found: 1) transcriptions of cellulase-related genes were not closely associated with measured differences in cellulase activity and 2) cellular energetics (intracellular ATP) correlated more closely to changes in specific cellulase activity. In T. fusca, CelR is thought to act as the primary regulator of cellulase gene expression by binding to a 14-bp inverted repeat: 50́9-(T)GGGAGCGCTCCC(A) that is upstream of many known cellulase genes. An efficient procedure for creating precise chromosomal gene replacements has been developed and this procedure was demonstrated by generating a celR deletion strain. Measurements of mRNA transcript levels in both the celR deletion strain and the wild-type strain indicated that the CelR potentially acts as a repressor for some cellulase genes and as an activator for other cellulase genes. Based on the protocol of disrupting celR gene, the direct conversion of untreated cellulosic biomass to 1-propanol in aerobic growth conditions using an engineered strain of T. fusca was demonstrated. Based upon computational predictions, a bifunctional butyraldehyde/alcohol dehydrogenase (encoded by adhE2) was added to T. fusca leading to production of 1-propanol during growth on glucose, cellobiose, cellulose (Avicel), switchgrass, and corn stover. The highest 1-propanol titer (0.48 g/L) was achieved for growth on switchgrass. The adaptive evolution of T. fusca was conducted to find a high cellulase-yield strain. The evolved strains of T. fusca were generated for two different scenarios: continuous exposure to cellobiose (strain muC with specialist phenotype) or alternating exposure to cellobiose and glucose (strain muS with generalist phenotype). Characterization of cellular phenotypes and whole genome re-sequencing were conducted for both the muC and muS strains and 18 and 14 point mutations in the muC and muS strains, respectively were verified. Among these mutations, the site mutation of Tfu_1867 was found to contribute the specialist phenotype and the site mutation of Tfu_0423 was found to contribute the generalist phenotype. The experiment results were used to test genome-scale metabolic model of T. fusca built in this study.

Download or read book Adsorption of Thermomonospora Fusca E3 and E5 and Trichoderma Reesei CBHI Cellulases on Cellulose and Silica written by Worakrit Suvajittanont and published by . This book was released on 1999 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Assaying the Activities of Thermomonospora Fusca E5 and Trichoderma Reesei CBHI Cellulases Bound to Polystyrene written by Sasithorn Kongruang and published by . This book was released on 1999 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this study the enzymatic activity of adsorbed Thermomonospora fusca E5 and Trichoderma reesei CBHI cellulases were investigated using fluorescence techniques. In particular, cellulases were allowed to contact hydrophobic polystyrene surfaces under conditions of different solution concentrations, and adsorption times. Each of these variables is known to have a potential effect on enzyme structure and activity at an interface. Enzymatic activity was measured after partial elution of the adsorbed layer with both protein-free buffer and the surfactant, dodecyltrimethylammonium bromide. For E5 at high concentration (0.5 mg/ml), adsorbed enzyme activity decreased about 20% in increasing adsorption time from 0.25 h to 24 h. At low concentration (0.001 mg/ml), adsorbed enzyme activity decreased by one order of magnitude during a 24 h period. CBHI layers lost activity only after a sufficiently long contact time with the surface, and this effect was not strongly dependent on enzyme concentrations in solution. These findings were explained with reference to structural changes undergone by adsorbed enzyme as a function of time and available interfacial area.

Download or read book Transcription Regulation of Cellulase Genes in Clostridium Thermocellum written by Yuan Zhang and published by . This book was released on 2018 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bergey s Manual of Systematic Bacteriology written by Aidan Parte and published by Springer Science & Business Media. This book was released on 2012-06-23 with total page 2102 pages. Available in PDF, EPUB and Kindle. Book excerpt: Includes a revised taxonomic outline for the Actinobacteria or the high G+C Gram positives is based upon the SILVA project as well as a description of greater than 200 genera in 49 families. Includes many medically and industrially important taxa.

Download or read book Genetics Abstracts written by and published by . This book was released on 2000 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microbial Gene Techniques Part B written by and published by Academic Press. This book was released on 1995-08-25 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The focus of the volume is on microbial cells, particularly eukaryotic microbes and bacteria, as well as plasmids and bacteriophages.* * Methods presented for ease of use and ready adaptation to new systems.* Detailed protocols included for:* Eukaryotic microbes - protozoan parasites (forward and reverse genetics, genome analysis), filamentous fungi (chromosome and gene analysis)* Yeast chromosomes - YACs, genome mapping, transcription factors, nucleosomes, recombination, RNA polymerase, pheromones.* Bacterial gene structure and regulation - E. coli (DNA methylation, mRNA characterization, gene regulation), B Subtilis (genetic mapping, chemotaxis), computer identification of genes.* Plasmids and bacteriophages - plasmid templates for transcription assays, plasmid replication: bacteriophage transcription, molecular genetic analysis using phages, phage assembly.

Download or read book Regulation of Phytochemicals by Molecular Techniques written by J.A. Saunders and published by Elsevier. This book was released on 2001-07-23 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: The papers assembled in this volume were originally presented at the joint meeting of the Phytochemical Society of North America and the Mid-Atlantic Plant Molecular Biology Society, in August 2000. The symposium from which these chapters were prepared was entitled "Regulation of Phytochemicals by Molecular Techniques" and was organised by James Saunders and Ben Matthews. This joint meeting was timely because of recent landmark advances in molecular biology and genomics as well as the renewed interest in phytochemistry as a rich source of nutraceuticals, drugs, and alternatives to synthetic agriculture pesticides. Progress in genome sequencing in plants such as Arabidopsis and rice has been remarkable, as have expressed sequence tag (EST) projects in other plants, including maize and soybean. Recently, private and public sector participants of the Human Genome Project announced that a rough draft of the human genome has been constructed. These advances directly influence phytochemical investigations by providing both insight and tools for exploring and manipulating genomes. The chapters cover a wide range of applications from molecular biology to phytochemistry, and from basic studies on promoters and gene expression to pathway regulation and engineering with transformed plants. A number of noteworthy aspects emerge from this volume: applications of molecular biology to phytochemical practical problems are succeeding; newly emerging molecular tools promise to open new doors to discovery; and remarkable progress has already occurred in phytochemical pathway engineering.

Download or read book written by עמיחי פרימן and published by Open University of Israel. This book was released on 1998 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: יחידה 1: ביוקטליזה: טכנולוגיה אנזימית.-- יחידה 2: מיקרוביולוגיה יישומית.-- יחידה 3: יישומים ביוטכנולוגיים של הנדסה גנטית.-- יחידה 4: נוגדנים ושימושיהם.-- יחידה 5: תרביות תאים.-- יחידה 6: ביוטכנולוגיה סביבתית.

Download or read book Biosynthesis and Biodegradation of Cellulose written by Candace H. Haigler and published by CRC Press. This book was released on 1990-12-20 with total page 713 pages. Available in PDF, EPUB and Kindle. Book excerpt: A gathering of articles bringing together knowledge of both the synthesis and degradation of a pervasive biological substance, cellulose. Topics include native cellulose; particle rosettes and terminal globules; microfibril biogenesis; synthesis in Acetobacter xylinum ; biodegradation measurement; e