Download or read book Regulation of Cell Behavior at the Cell Surface Interface written by Morgan M. Stanton and published by . This book was released on 2014 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: The growth and morphology of fibroblasts cultured on a physically and chemically modified surface was investigated. The need to understand cellular relationships with surface topography and chemistry is essential in the fields of biomedical engineering and biotechnology. It is well documented that mammalian cell behavior senses and responds to the surrounding micro- and nano- scale environment, but the research defining the chemistry, surface architecture, and material properties for control of this behavior is still in its infancy. The cell response to a substrate is complex, involving membrane proteins, extracellular matrix (ECM), cytoskeletal rearrangement, and changes in gene expression. Conventional cell culture is carried out on two-dimensional (2-D) cell culture platforms, such as polystyrene (PS) or glass, and forces cell behavior to adapt and adhere to an unnatural, planar environment. The biological behavior of these cells is used as a starting point for drug screening, implant design, and metabolic processes, but this is a misrepresentation of cells in their native environment. This discrepancy may be hampering biological research or initiating experimental efforts that are invalid. This body of work seeks to address these issues and contains established protocols for inexpensive, pseudo three-dimensional (3-D) culture scaffolds. The research described offers a multi-disciplinary approach for fabrication of biomaterials to achieve user defined or in vivo cell behavior using human fibroblasts. To provide insight into the design of alternative cell culture templates we have analyzed cell-surface interactions and characterized the surface properties. The substrates fabricated utilized micro-roughened surface topography with 2 0́3 6 [mu]m wide features and surface chemistry as a method for controlling cell behavior. Surface roughness was templated onto polydimethylsiloxane (PDMS) and PS. The fabricated polymer surfaces have been characterized by atomic force microscopy (AFM), contact angle goniometry, fluorescence microscopy, and infrared (IR) spectroscopy. Initial studies of the textured surface yielded a super-hydrophobic surface with a 154℗ʻ contact angle and high surface adhesion that was investigated using surface free energy calculations. This was followed by modification of the micro-roughness with self-assembled monolayers (SAMs), proteins, or thin films of polymer for use as a culture platform for cells. Cell behavior on the modified polymers was compared and analyzed against unmodified surfaces and tissue culture PS dishes. Cell morphology on rough PDMS surface was altered by the surface topography decreasing the average cell area to 1760 [mu]m2 compared to an average cell area of 3410 [mu]m2 on smooth PDMS. Gene expression changes were also noted with a 2.3 fold increase in the matrix metalloproteinase, MMP14, in cells on the rough surface compared to cells cultured on Petri dishes. Surface roughness was also combined with other surface modification methods for cell culture, including cell alignment and cell sheet engineering. 50 [mu]m wide lines of fibronectin (FN) patterned on the rough PDMS induced cell directionality while still maintaining a pseudo 3-D culture system creating the first cell culture surface of its kind. The micro-roughness was also templated onto PS and chemically modified with a thermo-responsive polymer. This novel surface produced confluent cell sheets that detached from the surface when cooled below 32℗ʻC. Cell sheets cultured on the modified PS surfaces had an increase in FN fibril formation stimulated by the surface roughness when compared to cell sheets detached from a smooth, control surface. The minor alterations to surface topology were proven to be effective in modifying cell biochemical response compared to cells cultured on flat substrates. Differences in surface topography and chemistry stimulated changes in cell adhesion, cytoskeletal arrangement, ECM composition, and gene expression. These cell properties were used as markers for comparison to native cell systems and other reports of 3 D culture scaffolds. The mechanism of altering cell response is discussed in each chapter with respect to the specific type of surface used and compared to cell response and behavior on planar culture systems. New fabrication procedures are described that include the incorporation of other surface modification techniques such as SAMs, surface patterning, and thermo-responsive polymer grafting with surface roughness for original cell culture platforms to mimic an in vivo environment. The research presented here demonstrates that micro- and nano- changes to surface topography have large impacts on the cell-surface relationship which have important implications for research and medical applications involving adherent cells.
Download or read book Principles of Cellular Engineering written by Michael R. King and published by Elsevier. This book was released on 2011-04-28 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive work discusses novel biomolecular surfaces that have been engineered to either control or measure cell function at the atomic, molecular, and cellular levels. Each chapter presents real results, concepts, and expert perspectives of how cells interact with biomolecular surfaces, with particular emphasis on interactions within complex mechanical environments such as in the cardiovascular system. In addition, the book provides detailed coverage of inflammation and cellular immune response as a useful model for how engineering concepts and tools may be effectively applied to complex systems in biomedicine.-Accessible to biologists looking for new ways to model their results and engineers interested in biomedical applications -Useful to researchers in biomaterials, inflammation, and vascular biology -Excellent resource for graduate students as a textbook in cell & tissue engineering or cell mechanics courses
Download or read book Surface and Interfacial Aspects of Cell Adhesion written by Alain Carré and published by CRC Press. This book was released on 2011-01-19 with total page 545 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell adhesion comes into play in almost all domains of life. The range of situations in which it occurs, involving organisms, living tissues, microorganisms or single cells, is endless. Cell adhesion is involved in the binding of a cell to a surface, extracellular matrix, or another cell using cell adhesion molecules. It is crucial in the formation and maintenance of coherent multicellular structures. Cell surface adhesion molecules (integrins, for example) which transmit information from the extracellular matrix to the cell play vital roles in numerous cellular processes. Some of these include: cell growth, differentiation, embryogenesis, immune cell transmigration and response, and cancer metastasis. Also cell adhesion is involved in most of pathological situations. This book is divided into four parts as follows: Part 1: Fundamentals of Cell Adhesion; Part 2: Methods to Study Cell Adhesion; Part 3: Surface Treatments to Control Cell Adhesion and Behavior; and Part 4: Cell Adhesion in Medicine and Therapy. A bountiful information is covered in this book which represents the cumulative wisdom of many world-renowned researchers( physicists, materials scientists, chemists and biologists) engaged in unraveling the mechanisms of cell adhesion and how to mitigate or control it. It quite patent from the topics covered in this book that the subject of cell adhesion is truly interdisciplinary. This book should be of great interest and value to anyone interested in cell adhesion which is vitally important to human life.
Download or read book Cell and Material Interface written by Nihal Engin Vrana and published by CRC Press. This book was released on 2018-09-03 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: A significant portion of biomedical applications necessitates the establishment of an interface between the cells of the patient and the components of the device. In many cases, such as in implants and engineered tissues, the interaction of the cells with the biomaterial is one of the main determinants of the success of the system. Cell and Material Interface: Advances in Tissue Engineering, Biosensor, Implant, and Imaging Technologies explores this interaction and its control at length scales ranging from the nano to the macro. Featuring contributions from leading molecular biologists, chemists, and material scientists, this authoritative reference: Presents practical examples of cell and material interface-based applications Reflects the interdisciplinary nature of bioengineering, covering topics such as biosensing, immunology, and controlled delivery Explains the role of the cell and material interface in the context of cardiac and skin tissue engineering, nanoparticles, natural polymers, and more Cell and Material Interface: Advances in Tissue Engineering, Biosensor, Implant, and Imaging Technologies addresses concepts essential to biomaterial production methods and cell and material interactions. The book provides a solid starting point for elucidating and exploiting the different aspects of cellular interactions with materials for biomedical engineering.
Download or read book Control of Cell Behavior by Engineering Substrata with Defined Surface Topography and Chemistry written by Rahul Singhvi and published by . This book was released on 1994 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Shape written by Wilfred D. Stein and published by . This book was released on 1989 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Mechanoresponse at Cell Material Interface written by Qiang Wei and published by Frontiers Media SA. This book was released on 2022-02-01 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomedical Nanostructures written by Kenneth Gonsalves and published by John Wiley & Sons. This book was released on 2007-11-09 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn to Use Nanoscale Materials to Design Novel Biomedical Devices and Applications Discover how to take full advantage of nanoscale materials in the design and fabrication of leading-edge biomedical devices. The authors introduce you to a variety of possible clinical applications such as drug delivery, diagnostics, and cancer therapy. In addition, the authors explore the interface between micron and nanoscale materials for the development of applications such as tissue engineering. Finally, they examine the mechanisms of cell interactions with material surfaces through the use of nanotechnology-based material processing and characterization methods. The text's three sections highlight its interdisciplinary approach: * Part One: Nanostructure Fabrication * Part Two: Bio-Nano Interfaces * Part Three: Clinical Applications of Nanostructures Among the key topics covered are nanotechnology in tissue regeneration; biomolecular engineering; receptor-ligand interactions; cell-biomaterial interactions; nanomaterials in diagnostics, drug delivery, and cancer therapy; and nano- and micron-level engineering and fabrication. Throughout the text, clear examples guide you through the chemistry and the processing involved in designing and developing nanoscale materials for biomedical devices. Each chapter begins with an introduction and ends with a conclusion highlighting the key points. In addition, references at the end of the chapter help you expand your research on any individual topic. In summary, this book helps biomedical researchers and engineers understand the physical phenomena that occur at the nanoscale in order to design novel cell-based constructs for a wide range of applications.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biophysical Regulation of Cell Motility by Adhesion Ligands and Growth Factors written by Gargi Maheshwari and published by . This book was released on 1999 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microsystems for Enhanced Control of Cell Behavior written by Andrés Díaz Lantada and published by Springer. This book was released on 2016-03-23 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook focuses on the entire development process of biomedical microsystems that promote special interactions with cells. Fundamentals of cell biology and mechanobiology are described as necessary preparatory input for design tasks. Advanced design, simulation, and micro/nanomanufacturing resources, whose combined use enables the development of biomedical microsystems capable of interacting at a cellular level, are covered in depth. A detailed series of chapters is then devoted to applications based on microsystems that offer enhanced cellular control, including microfluidic devices for diagnosis and therapy, cell-based sensors and actuators (smart biodevices), microstructured prostheses for improvement of biocompatibility, microstructured and microtextured cell culture matrices for promotion of cell growth and differentiation, electrophoretic microsystems for study of cell mechanics, microstructured and microtextured biodevices for study of cell adhesion and dynamics, and biomimetic microsystems (including organs-on-chips), among others. Challenges relating to the development of reliable in vitro biomimetic microsystems, the design and manufacture of complex geometries, and biofabrication are also discussed.
Download or read book The Adhesive Interaction of Cells written by David Garrod and published by Elsevier. This book was released on 1999-03-31 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of "The Adhesive Interaction of Cells" has been to assemble a series of reviews by leading international experts embracing many of the most important recent developments in this rapidly expanding field. The purpose of all biological research is to understand the form and function of living organisms and, by comprehending the normal, to find explanations and remedies for the abnormal and for disease conditions. The molecules involved in cell adhesion are of fundamental importance to the structure and function of all multicellular organisms. In this book, the contributors focus on the systems of vertebrates, especially mammals, since these are most relevant to human disease. It would have been equally possible to concentrate on developmental processes and adhesion in lower organisms. A major function of adhesion molecules is to bind cells to each other or to the extracellular matrix, but they are much more than "glue". Adhesions in animal tissues must be dynamic-forming, persisting, or declining in regulated fashion- to facilitate the mobility and turnover of tissue cells. Moreover, the majority of adhesion molecules are transmembrane molecules and thus provide links between the cells and their surroundings. This gives rise to another major function of adhesion molecules, the capacity to transduce signals across the hydrophobic barrier imposed by the plasma membrane. Such signal transduction is crucially important to many aspects of cellular function including the regulation of cell motility, gene expression, and differentiation. The work in this book progresses through four sections. Part I discusses the four major families of adhesion molecules themselves, the integrins (Green and Humphries), the cadherins (Stappert and Kemler), the selectins (Tedder et al.) and the immunoglobulin superfamily (Simmons); part 2 considers junctional complexes involved in cell interactions: focal adhesions and adherens junctions (Ben Ze'ev), desmosomes (Garrod et al.), and tight junctions (Citi and Cordenonsi). The signaling role of adhesion molecules is the focus of part 3, through integrins and the extracellular matrix (Edwards and Streuli), through platelet adhesion (Du and Ginsberg), and in the nervous system (Hemperley). In part 4, the aim is to show how adhesive phenomena contribute to important aspects of cell behavior and human health. Leukocyte trafficking (Haskard et al.), cancer metastasis (Marshall and Hart), cell migration (Paleck et al.), and implantation and placentation (Damsky et al.) are the topics considered in depth. The different sections are, of course, not mutually exclusive: it is both undesirable and impossible to separate structure from function when considering cell adhesion. Each chapter has its unique features, but some overlap is both invevitable and valuable since it provides different perspectives on closely related topics. We hope that the whole contributes a valuable and stimulating consideration of this important topic.
Download or read book Molecular Mechanisms in the Regulation of Cell Behavior written by Tissue Culture Association. Review Conference and published by . This book was released on 1987-01-01 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Polymeric Biomaterials for Tissue Regeneration written by Changyou Gao and published by Springer Nature. This book was released on 2024-02-01 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews state of the art of polymeric biomaterials for regenerative medicine and highlights advances in both fundamental science and clinical practice. It summarizes the latest techniques in polymeric scaffold fabrication, delivery carriers, physiochemical property modulation, as well as their influence on the adhesion and performance of biomolecules, cells, and tissues. It also describes methods for creating biofunctional surfaces/interfaces and subsequently modulating the host response to implantable materials. Lastly, it discusses the applications of biomaterials and constructs in soft-tissue regenerative medicine. It is a valuable resource for materials scientists and engineers wishing to identify research priorities to fulfill clinical needs and provides physicians with insights into emerging novel biomaterials. This integrated approach also offers engineering students a sense of the relevance of materials science in the development of novel therapeutic strategies. In the second edition, most of the chapters are updated according to the latest progress of this research field. A new chapter on nerve regeneration is also included.
Download or read book Bionanotechnology II written by David E. Reisner and published by CRC Press. This book was released on 2011-08-25 with total page 587 pages. Available in PDF, EPUB and Kindle. Book excerpt: The impact and importance of nanotechnology continues to grow, and nanomedicine and biotechnology have become areas of increased development. Biomedical engineers who work with biological processes and structures must have a deeply rooted understanding of the role of bionanotechnology, a rapidly evolving sector of the nanotechnology field. Bionanotechnology II: Global Prospects, a follow-up to the editor’s highly successful first volume, contains 26 entirely new contributions that provide a broad survey of research shaping this critical field. With coverage of technical and nontechnical areas, the book offers representative reporting on a wide variety of activity from around the world. It discusses the role of nanotechnology in novel medical devices, bioanalytical technologies, and nanobiomaterials. Topics discussed include: Emerging microscale technologies Bionanotech-based water treatment Tissue engineering and drug delivery Antimicrobial nanomaterials in the textile industry Bionanotechnology applications in plants and agriculture With contributions from researchers in Israel, Egypt, Iran, Jordan, Singapore, South Africa, Turkey, Thailand, Argentina, the United Kingdom, and the United States, this volume presents a worldwide perspective on some of the critical areas shaping bionanotechnology today.
Download or read book Research Awards Index written by and published by . This book was released on 1980 with total page 1230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Aspects of Cell Regulation written by G. H. Bourne and published by Elsevier. This book was released on 2013-11-06 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aspects of Cell Regulation deals with the various aspects of cellular control mechanisms. The book begins with a discussion of the role of hormones in the control of cell differentiation and biochemical control over the life of an organism. This is followed by separate chapters on an intracellular control problem, namely, the proper reproduction of cellular organelles and their apportionment between daughter cells at cell mitosis; cell mutants with different temporal order for macromolecular synthesis; and the control of a plant enzyme that converts triglycerides into carbohydrate during the early stages of postgerminative growth within seed cotyledon. Subsequent chapters deal with the possible role of mitochondria in changing the control mechanism of cells to that associated with carcinogenesis; the possibility of transforming genes being detectable in the genome after the transformation to neoplastic cell; the correlation between viruses and tumor cells; and whether viruses can originate from cells rather than coming before cells.
