Download or read book Gene Quantification written by Francois Ferre and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.
Download or read book Real time Data Standard Requirements written by Gerardus Blokdyk and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Real-time data Standard Requirements.
Download or read book Real Time Data Standard Requirements written by Gerardus Blokdyk and published by 5starcooks. This book was released on 2018-04-13 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: What are the key elements of your Real-time data performance improvement system, including your evaluation, organizational learning, and innovation processes? Are accountability and ownership for Real-time data clearly defined? How do we maintain Real-time data's Integrity? How can you negotiate Real-time data successfully with a stubborn boss, an irate client, or a deceitful coworker? What new services of functionality will be implemented next with Real-time data ? Defining, designing, creating, and implementing a process to solve a challenge or meet an objective is the most valuable role... In EVERY group, company, organization and department. Unless you are talking a one-time, single-use project, there should be a process. Whether that process is managed and implemented by humans, AI, or a combination of the two, it needs to be designed by someone with a complex enough perspective to ask the right questions. Someone capable of asking the right questions and step back and say, 'What are we really trying to accomplish here? And is there a different way to look at it?' This Self-Assessment empowers people to do just that - whether their title is entrepreneur, manager, consultant, (Vice-)President, CxO etc... - they are the people who rule the future. They are the person who asks the right questions to make Real-time data investments work better. This Real-time data All-Inclusive Self-Assessment enables You to be that person. All the tools you need to an in-depth Real-time data Self-Assessment. Featuring 703 new and updated case-based questions, organized into seven core areas of process design, this Self-Assessment will help you identify areas in which Real-time data improvements can be made. In using the questions you will be better able to: - diagnose Real-time data projects, initiatives, organizations, businesses and processes using accepted diagnostic standards and practices - implement evidence-based best practice strategies aligned with overall goals - integrate recent advances in Real-time data and process design strategies into practice according to best practice guidelines Using a Self-Assessment tool known as the Real-time data Scorecard, you will develop a clear picture of which Real-time data areas need attention. Your purchase includes access details to the Real-time data self-assessment dashboard download which gives you your dynamically prioritized projects-ready tool and shows your organization exactly what to do next. Your exclusive instant access details can be found in your book.
Download or read book Quantitative Real Time PCR written by Roberto Biassoni and published by Humana. This book was released on 2014-04-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.
Download or read book Rapid Cycle Real Time PCR written by S. Meuer and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first comprehensive treatise on Rapid Cycle Real-Time PCR. With amplification times of 15-30 minutes of on-line detection and analysis, nucleic acid quantification of mutation analysis finally becomes a routine, powerful and rapid method. Focusing primarily on the LightCycler, an instrument that combines Rapid Cycle PCR with fluorescent monitoring, this technology provides convenient analysis by melting temperatures. PCR products can be identified by product Tm, and single base mismatches can easily be genotyped by probe Tm. Methods chapters detail the theory behind quantification of mutation analysis; the design of synthesis of fluorescent hybridization probes of the preparation of template DNA. Application chapters apply nucleid acid quantification to infectious organisms of intracellular messengers and mutation detection to somatic of acquired mutations.
Download or read book Registries for Evaluating Patient Outcomes written by Agency for Healthcare Research and Quality/AHRQ and published by Government Printing Office. This book was released on 2014-04-01 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: This User’s Guide is intended to support the design, implementation, analysis, interpretation, and quality evaluation of registries created to increase understanding of patient outcomes. For the purposes of this guide, a patient registry is an organized system that uses observational study methods to collect uniform data (clinical and other) to evaluate specified outcomes for a population defined by a particular disease, condition, or exposure, and that serves one or more predetermined scientific, clinical, or policy purposes. A registry database is a file (or files) derived from the registry. Although registries can serve many purposes, this guide focuses on registries created for one or more of the following purposes: to describe the natural history of disease, to determine clinical effectiveness or cost-effectiveness of health care products and services, to measure or monitor safety and harm, and/or to measure quality of care. Registries are classified according to how their populations are defined. For example, product registries include patients who have been exposed to biopharmaceutical products or medical devices. Health services registries consist of patients who have had a common procedure, clinical encounter, or hospitalization. Disease or condition registries are defined by patients having the same diagnosis, such as cystic fibrosis or heart failure. The User’s Guide was created by researchers affiliated with AHRQ’s Effective Health Care Program, particularly those who participated in AHRQ’s DEcIDE (Developing Evidence to Inform Decisions About Effectiveness) program. Chapters were subject to multiple internal and external independent reviews.
Download or read book Site Reliability Engineering written by Niall Richard Murphy and published by "O'Reilly Media, Inc.". This book was released on 2016-03-23 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: The overwhelming majority of a software system’s lifespan is spent in use, not in design or implementation. So, why does conventional wisdom insist that software engineers focus primarily on the design and development of large-scale computing systems? In this collection of essays and articles, key members of Google’s Site Reliability Team explain how and why their commitment to the entire lifecycle has enabled the company to successfully build, deploy, monitor, and maintain some of the largest software systems in the world. You’ll learn the principles and practices that enable Google engineers to make systems more scalable, reliable, and efficient—lessons directly applicable to your organization. This book is divided into four sections: Introduction—Learn what site reliability engineering is and why it differs from conventional IT industry practices Principles—Examine the patterns, behaviors, and areas of concern that influence the work of a site reliability engineer (SRE) Practices—Understand the theory and practice of an SRE’s day-to-day work: building and operating large distributed computing systems Management—Explore Google's best practices for training, communication, and meetings that your organization can use
Download or read book Generative Artificial Intelligence and Ethics Standards Guidelines and Best Practices written by Gaur, Loveleen and published by IGI Global. This book was released on 2024-10-10 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid advancement of generative artificial intelligence (AI) has brought about significant ethical challenges. As machines become more adept at creating human-like content, concerns about misuse, bias, privacy, and accountability have emerged. Without clear guidelines and regulations, there is a risk of unethical use, such as creating deepfake videos or disseminating misinformation, which could have severe societal consequences. Additionally, questions about intellectual property rights and the ownership of AI-generated creations still need to be solved, further complicating the ethical landscape. The book, Generative Artificial Intelligence and Ethics: Standards, Guidelines, and Best Practices, comprehensively solves these ethical challenges. By providing insights into the historical development and key milestones of Generative AI, the book lays a foundation for understanding its complex ethical implications. It examines existing ethical frameworks and proposes new ones tailored to AI's unique characteristics, helping readers apply traditional ethics to AI development and deployment.
Download or read book Handbook of Research on E Business Standards and Protocols Documents Data and Advanced Web Technologies written by Kajan, Ejub and published by IGI Global. This book was released on 2012-02-29 with total page 1021 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electronic business is a major force shaping the digital world. Yet, despite of years of research and standardization efforts, many problems persist that prevent e-business from achieving its full potential. Problems arise from different data vocabularies, classification schemas, document names, structures, exchange formats and their varying roles in business processes. Non-standardized business terminology, lack of common acceptable and understandable processes (grammar), and lack of common dialog rules (protocols) create barriers to improving electronic business processes. Handbook of Research on E-Business Standards and Protocols: Documents, Data and Advanced Web Technologies contains an overview of new achievements in the field of e-business standards and protocols, offers in-depth analysis of and research on the development and deployment of cutting-edge applications, and provides insight into future trends. This book unites new research that promotes harmony and agreement in business processes and attempts to choreograph business protocols and orchestrate semantic alignment between their vocabularies and grammar. Additionally, this Handbook of Research discusses new approaches to improving standards and protocols, which include the use of intelligent agents and Semantic Web technology.
Download or read book Sharing Clinical Trial Data written by Institute of Medicine and published by National Academies Press. This book was released on 2015-04-20 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Data sharing can accelerate new discoveries by avoiding duplicative trials, stimulating new ideas for research, and enabling the maximal scientific knowledge and benefits to be gained from the efforts of clinical trial participants and investigators. At the same time, sharing clinical trial data presents risks, burdens, and challenges. These include the need to protect the privacy and honor the consent of clinical trial participants; safeguard the legitimate economic interests of sponsors; and guard against invalid secondary analyses, which could undermine trust in clinical trials or otherwise harm public health. Sharing Clinical Trial Data presents activities and strategies for the responsible sharing of clinical trial data. With the goal of increasing scientific knowledge to lead to better therapies for patients, this book identifies guiding principles and makes recommendations to maximize the benefits and minimize risks. This report offers guidance on the types of clinical trial data available at different points in the process, the points in the process at which each type of data should be shared, methods for sharing data, what groups should have access to data, and future knowledge and infrastructure needs. Responsible sharing of clinical trial data will allow other investigators to replicate published findings and carry out additional analyses, strengthen the evidence base for regulatory and clinical decisions, and increase the scientific knowledge gained from investments by the funders of clinical trials. The recommendations of Sharing Clinical Trial Data will be useful both now and well into the future as improved sharing of data leads to a stronger evidence base for treatment. This book will be of interest to stakeholders across the spectrum of research-from funders, to researchers, to journals, to physicians, and ultimately, to patients.
Download or read book Frontiers in Massive Data Analysis written by National Research Council and published by National Academies Press. This book was released on 2013-09-03 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: Data mining of massive data sets is transforming the way we think about crisis response, marketing, entertainment, cybersecurity and national intelligence. Collections of documents, images, videos, and networks are being thought of not merely as bit strings to be stored, indexed, and retrieved, but as potential sources of discovery and knowledge, requiring sophisticated analysis techniques that go far beyond classical indexing and keyword counting, aiming to find relational and semantic interpretations of the phenomena underlying the data. Frontiers in Massive Data Analysis examines the frontier of analyzing massive amounts of data, whether in a static database or streaming through a system. Data at that scale-terabytes and petabytes-is increasingly common in science (e.g., particle physics, remote sensing, genomics), Internet commerce, business analytics, national security, communications, and elsewhere. The tools that work to infer knowledge from data at smaller scales do not necessarily work, or work well, at such massive scale. New tools, skills, and approaches are necessary, and this report identifies many of them, plus promising research directions to explore. Frontiers in Massive Data Analysis discusses pitfalls in trying to infer knowledge from massive data, and it characterizes seven major classes of computation that are common in the analysis of massive data. Overall, this report illustrates the cross-disciplinary knowledge-from computer science, statistics, machine learning, and application disciplines-that must be brought to bear to make useful inferences from massive data.
Download or read book Scientific and Technical Aerospace Reports written by and published by . This book was released on 1994 with total page 1028 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lists citations with abstracts for aerospace related reports obtained from world wide sources and announces documents that have recently been entered into the NASA Scientific and Technical Information Database.
Download or read book The Reliability and Validity of Real time PCR Data in Biomedical Sciences written by Yasir Khan and published by GRIN Verlag. This book was released on 2015-01-28 with total page 15 pages. Available in PDF, EPUB and Kindle. Book excerpt: Seminar paper from the year 2014 in the subject Mathematics - Statistics, grade: B, National University of Modern Languages, Islamabad (NUML), course: Statistics, language: English, abstract: Despite a fairly broad implementation and application of real-time PCR, there still exists a vacuum in determining the correct procedures for the examination of quantitative real-time PCR; more explicitly, there is a need to determine appropriate procedures to attain the right kind of statistical treatment. In today’s various methods of data analysis, the key statistical inferences are not as exclusive as required like confidence interval. This paper presents and tends to relate four statistical models and approaches on the basis of standard curve method and methods for data analysis. The first approach developed a multiple regression analysis model for the determination of ∆∆Ct directly from the approximation of interface of gene and treatment paraphernalia. The second approach used the analysis of covariance i.e. ANCOVA model where the derivation of ∆∆Ct could be made through the sequential evaluation and analysis of effects of concurrent variables. The remainder of the models chiefly involves the calculation of ∆Ct subsequently connected through the non-parametric comparable Wilcoxon test and a two group T-test. Moreover, a data quality control model was established, which was then applied through the SAS programs determined for all of the aforementioned approaches; analyzed data output was also presented for a sample set. The SAS programs were used to put forward practical statistical solutions for real-time PCR data while the programs were also utilized to analyze a sample dataset. After a comprehensive analysis conducted through the approaches and models mentioned above, similar results were obtained.
Download or read book Fundamentals of Clinical Data Science written by Pieter Kubben and published by Springer. This book was released on 2018-12-21 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book comprehensively covers the fundamentals of clinical data science, focusing on data collection, modelling and clinical applications. Topics covered in the first section on data collection include: data sources, data at scale (big data), data stewardship (FAIR data) and related privacy concerns. Aspects of predictive modelling using techniques such as classification, regression or clustering, and prediction model validation will be covered in the second section. The third section covers aspects of (mobile) clinical decision support systems, operational excellence and value-based healthcare. Fundamentals of Clinical Data Science is an essential resource for healthcare professionals and IT consultants intending to develop and refine their skills in personalized medicine, using solutions based on large datasets from electronic health records or telemonitoring programmes. The book’s promise is “no math, no code”and will explain the topics in a style that is optimized for a healthcare audience.
Download or read book Voting System Standards written by United States. Federal Election Commission and published by . This book was released on 2002 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Quality assurance Data Comparison to Water quality Standards and Site Considerations for Total Dissolved Gas and Water Temperature Lower Columbia River Oregon and Washington 2001 written by Dwight Q. Tanner and published by . This book was released on 2002 with total page 24 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Artificial Intelligence Enabled Digital Twin for Smart Manufacturing written by Amit Kumar Tyagi and published by John Wiley & Sons. This book was released on 2024-09-11 with total page 628 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential book on the applications of AI and digital twin technology in the smart manufacturing sector. In the rapidly evolving landscape of modern manufacturing, the integration of cutting-edge technologies has become imperative for businesses to remain competitive and adaptive. Among these technologies, Artificial Intelligence (AI) stands out as a transformative force, revolutionizing traditional manufacturing processes and making the way for the era of smart manufacturing. At the heart of this technological revolution lies the concept of the Digital Twin—an innovative approach that bridges the physical and digital realms of manufacturing. By creating a virtual representation of physical assets, processes, and systems, organizations can gain unprecedented insights, optimize operations, and enhance decision-making capabilities. This timely book explores the convergence of AI and Digital Twin technologies to empower smart manufacturing initiatives. Through a comprehensive examination of principles, methodologies, and practical applications, it explains the transformative potential of AI-enabled Digital Twins across various facets of the manufacturing lifecycle. From design and prototyping to production and maintenance, AI-enabled Digital Twins offer multifaceted advantages that redefine traditional paradigms. By leveraging AI algorithms for data analysis, predictive modeling, and autonomous optimization, manufacturers can achieve unparalleled levels of efficiency, quality, and agility. This book explains how AI enhances the capabilities of Digital Twins by creating a powerful tool that can optimize production processes, improve product quality, and streamline operations. Note that the Digital Twin in this context is a virtual representation of a physical manufacturing system, including machines, processes, and products. It continuously collects real-time data from sensors and other sources, allowing it to mirror the physical system’s behavior and performance. What sets this Digital Twin apart is the incorporation of AI algorithms and machine learning techniques that enable it to analyze and predict outcomes, recommend improvements, and autonomously make adjustments to enhance manufacturing efficiency. This book outlines essential elements, like real-time monitoring of machines, predictive analytics of machines and data, optimization of the resources, quality control of the product, resource management, decision support (timely or quickly accurate decisions). Moreover, this book elucidates the symbiotic relationship between AI and Digital Twins, highlighting how AI augments the capabilities of Digital Twins by infusing them with intelligence, adaptability, and autonomy. Hence, this book promises to enhance competitiveness, reduce operational costs, and facilitate innovation in the manufacturing industry. By harnessing AI’s capabilities in conjunction with Digital Twins, manufacturers can achieve a more agile and responsive production environment, ultimately driving the evolution of smart factories and Industry 4.0/5.0. Audience This book has a wide audience in computer science, artificial intelligence, and manufacturing engineering, as well as engineers in a variety of industrial manufacturing industries. It will also appeal to economists and policymakers working on the circular economy, clean tech investors, industrial decision-makers, and environmental professionals.
