Download or read book Rational Design of Novel Calcium binding Proteins written by Wei Yang and published by . This book was released on 2001 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Novel Calcium Binding Proteins written by Claus W. Heizmann and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: A wide variety of hormones, neurotransmitters and growth factors exert their cellular effects by reacting first with membrane receptors resulting in an increase of intracellular calcium and the cellular response. The calcium signal in the cell is mediated by the high-affinity calcium binding proteins (characterized by the EF-hand structural element), and by the calcium and phospholipid dependent proteins. Many of these have been discovered most recently. Their purification, distribution, protein and gene structures as well as their physiological roles are discussed. The book is of interest to biochemists and molecular biologists as well as to clinicians and the pharmaceutical industry who can apply the results in this field.
Download or read book Lovsangbog for Frederiksborg l rde Skole written by and published by . This book was released on 1883 with total page 30 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Calcium Binding Protein Protocols written by Hans J. Vogel and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.
Download or read book Novel Calcium Binding Proteins written by Claus W Heizmann and published by . This book was released on 1991-03-28 with total page 640 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Calcium Binding Protein Protocols written by Hans J. Vogel and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.
Download or read book Calcium Binding Proteins written by Eugene Permyakov and published by John Wiley & Sons. This book was released on 2011-03-21 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium Binding Proteins explains the unique and highly diverse functions of calcium in biology, which are realized by calcium binding proteins. The structures and physical characteristics of these calcium binding proteins are described, as well as their functions and general patterns of their evolution. Techniques that underlie the description of proteins are discussed, including NMR, circular dichroism, optical rotatory dispersion spectroscopy, calorimetry,and crystallography. The book discusses the patterns of bochmical phenomena such as calcium homeostasis, mineralization, and cell signaling that involve specific proteins. It summarizes ongoing research and presents general hypotheses that help to focus future research, and also provides a conceptual framework and a description of the underlying techniques that permits someone entering the field to become conversant.
Download or read book Rational Design of Faster Associating and Tighter Binding Protein Complexes written by Tzvia Selzer and published by . This book was released on 2001 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Calcium binding Proteins in Normal and Transformed Cells written by C. W. Heizmann and published by . This book was released on 1994 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Calcium binding Proteins in Health and Disease written by Anthony W. Norman and published by . This book was released on 1987 with total page 629 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Calcium binding Proteins 1 written by Hiroshi Kawasaki and published by . This book was released on 1994 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Guidebook to the Calcium binding Proteins written by Marco R. Celio and published by Sambrook and Tooze Publication at Oxford University Press. This book was released on 1996 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Each entry includes data on isolation/identification, alternative names, genes and sequences, proteins, antibodies, and biological activities.
Download or read book Calcium Binding Proteins of the EF Hand Superfamily written by Claus W. Heizmann and published by . This book was released on 2019 with total page 779 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry.
Download or read book Calcium binding Proteins written by Marvin P. Thompson and published by C R C Press Reprints. This book was released on 1988 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Rational Approach to Understanding the Relationship of Sequence Structure and Function in EF Hand Calcium binding Proteins written by Melanie Renee Nelson and published by . This book was released on 1999 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: EF-hand calcium-binding proteins are a large and diverse family of proteins, involved in many critical biological functions. The members of this family all contain multiple copies of the helix-loop-helix EF-hand motif. How this conserved motif is adapted to a wide-range of structures is not understood, even for the relatively well-studied classical signal transducers such as calmodulin and classical signal modulators such as calbindin D 9k . Calmodulin and calbindin D9k have similar sequences and apo structures, yet respond very differently to the calcium signal. This dissertation uses site-directed mutagenesis to address the question of how two similar proteins such as these can respond so differently to the same signal. The ability of these mutagenesis studies to provide new information about how sequence determines structure in this family depends upon a careful mutant design process, which in turn relies upon the efficient use of the information already available about these proteins. Therefore, the design and implementation of a relational database to organize and interconnect information from the literature with the data about the sequences and structures of EF-hand calcium-binding proteins is presented, and a thorough analysis of the relevant protein structures is performed. Information from the database and the structural analyses is used to design limited-site mutants to test hypotheses about how specific differences in the sequences of calbindin D 9k and calmodulin dictate differences in their structures and properties. Two more extensive multiple-site mutants are also designed, with the ultimate goal of re-engineering the response of calbindin D9k to the calcium signal, and making it more similar to the response exhibited by calmodulin. A structural assay is developed to identify the mutants most likely to have significant structural effects, and the assay is applied to three of the limited-site mutants, culminating in the structure determination of the apo state of one of these mutants, F36G. The structural effects of this mutation are consistent with the hypothesis it was designed to test, but also include perturbations not predicted by the mutant design, illustrating the value of studies such as this in elucidating how the sequence of a protein determines its structure.
Download or read book Rational Design Binding Studies and Crystal Structure Evaluation of the First Ligand Targeting the Dimerization Interface of the 14 3 3 Adapter Protein written by and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Computational Studies Nanotechnology and Solution Thermodynamics of Polymer Systems written by Mark D. Dadmun and published by Springer Science & Business Media. This book was released on 2007-05-08 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is the published version of many ofthe talks presented at two symposiums held as part of the Southeast Regional Meeting of the American Chemical Society (SERMACS) in Knoxville, TN in October, 1999. The Symposiums, entitled Solution Thermodynamics of Polymers and Computational Polymer Science and Nanotechnology, provided outlets to present and discuss problems of current interest to polymer scientists. It was, thus, decided to publish both proceedings in a single volume. The first part of this collection contains printed versions of six of the ten talks presented at the Symposium on Solution Thermodynamics of Polymers organized by Yuri B. Melnichenko and W. Alexander Van Hook. The two sessions, further described below, stimulated interesting and provocative discussions. Although not every author chose to contribute to the proceedings volume, the papers that are included faithfully represent the scope and quality of the symposium. The remaining two sections are based on the symposium on Computational Polymer Science and Nanotechnology organized by Mark D. Dadmun, Bobby G. Sumpter, and Don W. Noid. A diverse and distinguished group of polymer and materials scientists, biochemists, chemists and physicists met to discuss recent research in the broad field of computational polymer science and nanotechnology. The two-day oral session was also complemented by a number of poster presentations. The first article of this section is on the important subject of polymer blends. M. D.
