Download or read book Quantum Dots as Fluorescent Probes for in Vitro Cellular Imaging written by Milana Coorg Vasudev and published by . This book was released on 2006 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Quantum Dots written by Ameenah Al-Ahmadi and published by IntechOpen. This book was released on 2012-04-04 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book “Quantum dots: A variety of a new applications” provides some collections of practical applications of quantum dots. This book is divided into four sections. In section 1 a review of the thermo-optical characterization of CdSe/ZnS core-shell nanocrystal solutions was performed. The Thermal Lens (TL) technique was used, and the thermal self-phase Modulation (TSPM) technique was adopted as the simplest alternative method. Section 2 includes five chapters where novel optical and lasing application are discussed. In section 3 four examples of quantum dot system for different applications in electronics are given. Section 4 provides three examples of using quantum dot system for biological applications. This is a collaborative book sharing and providing fundamental research such as the one conducted in Physics, Chemistry, Biology, Material Science, Medicine with a base text that could serve as a reference in research by presenting up-to-date research work on the field of quantum dot systems.

Download or read book Quantum Dots Applications in Biology written by Adriana Fontes and published by Humana Press. This book was released on 2014-08-08 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Quantum Dots: Applications in Biology, Second Edition, expert researchers in the field detail consolidated approaches as well as new trends in the field. Organized into five parts, the first part comprises an introduction on Quantum Dots (QDs) as fluorescent probes in Life Sciences. While the second section covers important features about QDs ́ preparative processes and characterizations for their successful application as fluorophores. The third part presents main aspects related to QDs methods applied to live cells and tissues. The fourth section focuses on QDs experiments in small animals and the fifth part demonstrates the versatility of QDs in a set of FRET applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, In Quantum Dots: Applications in Biology, Second Edition aids scientists in continuing to study QDs by providing information about methods and protocols helping to expand their research.

Download or read book Semiconductor Quantum Dots And Rods For In Vivo Imaging And Cancer Phototherapy written by Maoquan Chu and published by #N/A. This book was released on 2017-06-19 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer is fast becoming one of the main causes of death worldwide. Unfortunately many cases are diagnosed at an advanced incurable stage, and these lives are usually lost. Early diagnosis and treatment are very important for increasing disease curability. In recent years, novel techniques for cancer diagnosis and therapy have been developed, and nanobiomedicine appears to show the most promising results.The application of nanotechnology to biology and medicine in cancer diagnosis is termed nanobiomedicine. Nanoparticles 1-100 nm in size usually have unique physical and/or chemical properties, and this has attracted great attention in the cancer research. Preparation and biomedical applications of the nanoparticles are key components in nanobiomedicine. Semiconductor nanocrystals, including quantum dots (QDs) and quantum rods (QRs), have been extensively investigated for drug delivery, biomedical imaging and tumor target therapy.In Semiconductor Quantum Dots and Rods for In Vivo Imaging and Cancer Phototherapy, the QD and QR optical properties, sentinel lymph node mapping, in vivo tumor target imaging, self-illuminating QDs for in vivo imaging, in vivo cancer photothermal therapy and photodynamic therapy, QD-graphene nanosheet, and QD-magnetic hybrid nanocomposites for bioimaging and cancer therapy are discussed. This book may interest under- and postgraduate students in the field of bioengineering (especially cancer phototherapy) and medical professions alike.

Download or read book Near infrared Emitting Quantum Dots for Cellular and Vascular Fluorescent Labeling in in Vivo Multiplexed Imaging Studies written by Juwell Wendy Wu and published by . This book was released on 2011 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: In vivo multimodal, multiplexed microscopy allows real-time observation of hematopoietic cells, their stem and progenitor cells and metastatic cancer cells in their native bone marrow (BM) environment. Multiplexing has made possible detailed studies of the BM's microarchitecture, which helps define the niche of these cells; it has nonetheless been limited by the paucity of suitable probes fluorescent in the near-infrared spectrum that is favored by tissue optics. This project attempts to address this problem by developing cellular and vascular fluorescent imaging probes comprised of semiconductor nanocrystals, or quantum dots (QDs), with tunable fluorescence between 65o-8oonm and exhibiting photostability, robust quantum yield and narrow fluorescence profiles that are critical for such applications. The synthesis of alloyed CdTexSe1 x QDs will be detailed in the thesis. Reproducibility and workability in subsequent steps are emphasized in the methods. Special attention is also paid to the difference between working with alloyed versus single semiconductor QDs, especially the need to achieve physical and spectral uniformity when composition and its gradient are also variable. The steps for creating biological probes from these QD fluorophores are also described. They include overcoating, water solubilization and functionalization for cellular uptake and vascular retention. Finally, the thesis returns to its motivation and reports novel methods, developed using NIR QD vascular imaging probes, for visualizing in vivo 3-D imaging data of the murine BM and characterizing the tissue's architecture. Measuring the Euclidean distance between BM osteoblasts and blood vessels is presented to exemplify a potential platform for describing the geographic relationships between cells, molecules and structural components in any tissue.

Download or read book IN VITRO IMAGING USING STABLE WATER DISPERSIBLE LUMINESCENT SI QUANTUM DOTS CAPPED WITH VARIOUS CELLULAR TARGETING AGENTS written by Chen-An Tien and published by . This book was released on 2010 with total page 60 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis presents research on the synthesis, functionalization, and biological applications of silicon nanocrystals, also called silicon quantum dots (Si QDs). Over the past 20 years, semiconductor nanocrystals (also known as quantum dots or QDs) have been investigated for applications ranging from electronic materials science to biological imaging. The outstanding optical properties of QDs, which include stable luminescence, tunable luminescence color, modifiable surface characteristics, and the potential to interface with biologically relevant molecules, have made QDs a good replacement for organic dyes in many applications. Silicon nanocrystals show many of the same useful optical properties but lower toxicity than cadmium based quantum dots for biological applications.^In this thesis, I focus on the biological applications of silicon nanocrystals in fluorescent biosensing and cellular labeling applications. Conventional quantum dots have great potential in cancer-related imaging and diagnostic applications; however, these applications are limited by concerns about the inherent toxicity of their core materials (e.g. cadmium, lead). Virtually all imaging applications require conjugation of the imaging agent to a biologically active molecule to achieve selective uptake or binding. Here, we report a study of biocompatible silicon quantum dots covalently attached to biomolecules including lysine, folate, anti-mesothelin, and transferrin. The particles possess desirable physical properties, surface chemistry, and optical properties. Folate- and anti-mesothelin-conjugated silicon quantum dots show selective uptake into Panc-1 cells.^This study contributes to the preclinical evaluation of silicon quantum dots and further demonstrates their potential as an imaging agent for cancer applications. Major bionanotechnology research issues in quantum dot synthesis include the stabilization of their optical properties using various coating and encapsulation strategies, and advancing the rational design of constructs containing them to optimize overall size, surface chemistry, and composition in order to minimize potential toxicity and overcome biological barriers. Conventional cadmium- and lead-based quantum dots are normally coated, because their degradation may result in the release of toxic heavy metal ions. For in vivo use, they must be cleared from the body without degradation. Freestanding silicon quantum dots are expected to biodegrade to non-toxic products (e.g. silicic acid); however they have not been evaluated in biodegradable nanocarriers.^Previous work from our group has encapsulated them with non-toxic, but non-biodegradable phospholipid-polyethylene glycol surfactants. Here, we report the development of chitosan-coated silicon nanoparticles (CSi QDs). Evaluation of the physicochemical and optical properties of the CSi QDs shows that they remain optically active in aqueous media. The chitosan coating renders silicon quantum dots stable in aqueous biological media and useful for biological applications such as cellular imaging with single and two photon excitation. The particles are also degradable when incubated at physiological temperature. These results open the door for a new generation of silicon quantum dots that may have a wide variety of applications derived from the flexibility of chitosan.

Download or read book Fluorescence Imaging and Biological Quantification written by Raquel Seruca and published by CRC Press. This book was released on 2017-09-06 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology.

Download or read book Cadmium Telluride Quantum Dots written by John Donegan and published by CRC Press. This book was released on 2013-12-03 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last two decades, semiconductor quantum dots—small colloidal nanoparticles—have garnered a great deal of scientific interest because of their unique properties. Among nanomaterials, CdTe holds special technological importance as the only known II–VI material that can form conventional p–n junctions. This makes CdTe very important for the development of novel optoelectronic devices such as light-emitting diodes, solar cells, and lasers. Moreover, the demand for water-compatible light emitters and the most common biological buffers give CdTe quantum dots fields a veritable edge in biolabeling and bioimaging. Cadmium Telluride Quantum Dots: Advances and Applications focuses on CdTe quantum dots and addresses their synthesis, assembly, optical properties, and applications in biology and medicine. It makes for a very informative reading for anyone involved in nanotechnology and will also benefit those scientists who are looking for a comprehensive account on the current state of quantum dot–related research.

Download or read book Near Infrared Emitting Nanoparticles for Biomedical Applications written by Antonio Benayas and published by Springer Nature. This book was released on 2020-04-13 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: ​This book analyzes and evaluates the growing field of light-emitting nanoprobes as contrast agents for in vivo imaging and sensing. It is a comprehensive resource that critically analyzes the state of the art in an interdisciplinary manner, with a special focus on the shift of emission wavelengths into the near-infrared (NIR) spectral region (ranging from 0.7 to 2 microns), which has greatly contributed to the latest advances in biomedical imaging and sensing. This book discusses merits of different contrast agents at nanoscale, and how their unique chemical and structural properties lead to the emission and interaction of light within the NIR window. Both the NIR-emitting materials and various surface modification strategies governing their interactions with the biological system at the “nano” level are discussed. Furthermore, different experimental techniques and protocols for NIR-light-based in vivo imaging and sensing are addressed to shed light on further understanding of the advantages and limitations of each category of these nanoprobes. Assembles the state of the art heretofore appearing in scientific literature into a comprehensive, multi-perspective guidebook on near infrared-emitting nanomaterials in an assortment of biomedical applications; Explains the physical, chemical, and biological phenomena underlying near infrared-emitting nanomaterials for biomedical applications; Presents conceptual and experimental approaches surrounding a unique spectral range of light emission from nanosized contrast agents, while offering a clear explanation of basic and general phenomena regarding the interaction between light and biological tissues, such as absorption, scattering and autofluorescence.

Download or read book Quantum Dots written by Ameenah Al-Ahmadi and published by BoD – Books on Demand. This book was released on 2012-04-04 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book “Quantum dots: A variety of a new applications” provides some collections of practical applications of quantum dots. This book is divided into four sections. In section 1 a review of the thermo-optical characterization of CdSe/ZnS core-shell nanocrystal solutions was performed. The Thermal Lens (TL) technique was used, and the thermal self-phase Modulation (TSPM) technique was adopted as the simplest alternative method. Section 2 includes five chapters where novel optical and lasing application are discussed. In section 3 four examples of quantum dot system for different applications in electronics are given. Section 4 provides three examples of using quantum dot system for biological applications. This is a collaborative book sharing and providing fundamental research such as the one conducted in Physics, Chemistry, Biology, Material Science, Medicine with a base text that could serve as a reference in research by presenting up-to-date research work on the field of quantum dot systems.

Download or read book West Aurora High School Aurora Illinois Personal Histories 1942 1954 Class of 1942 written by and published by . This book was released on 1954 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fluorescent Imaging in Medicinal Chemistry written by Zhen Cheng and published by Springer Nature. This book was released on 2020-05-28 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the most recent developments of fluorescent imaging techniques for medicinal chemistry research and biomedical applications, including cell imaging, in vitro diagnosis and in vivo imaging. Fluorescent imaging techniques play an important role in basic research, drug discovery and clinical translation. They have great impact to many fields including chemical biology, cell biology, medical imaging, cancer diagnosis and treatment, pharmaceutical science, among others, and they have facilitated our understanding of diseases and helped to develop many novel powerful tools for imaging and treatment of diseases. This book will appeal to scientists from numerous fields such as chemistry, pharmaceutical science, biology, materials science, and medicine, and it will serve as a very useful and handy resource for readers with different levels of scientific knowledge, ranging from entry level to professional level.

Download or read book Quantum Dots written by Inamuddin and published by Materials Research Forum LLC. This book was released on 2021-04-05 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book provides a thorough survey of current research in quantum dots synthesis, properties, and applications. The unique properties of these new nanomaterials offer multifunctional applications in such fields as photovoltaics, light-emitting diodes, field-effect transistors, lasers, photodetectors, solar cells, biomedical diagnostics and quantum computing. Keywords: Quantum Dots (QD), Photovoltaics, Light-emitting Diodes, Field-effect Transistors, Lasers, Photodetectors, Solar Cells, Biomedical Diagnostics, Quantum Computing, QD Synthesis, Carbon QDs, Graphene QDs, QD Sensors, Supercapacitors, Magnetic Quantum Dots, Cellular/Molecular Separation, Chromatographic Separation Column, Photostability, Luminescence of Carbon QDs, QD Materials for Water Treatment, Semiconductor Quantum Dots, QD Drug Delivery, Antibacterial Quantum Dots.

Download or read book Fluorescence Methods for Investigation of Living Cells and Microorganisms written by Natalia Grigoryeva and published by BoD – Books on Demand. This book was released on 2020-09 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.

Download or read book Development of Fluorescent Semi conductor Nanocrystal Conjugates for in Vitro and in Vivo Imaging Applications written by Hee-Sun Han (Ph. D.) and published by . This book was released on 2012 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: Semiconductor nanocrystals, also known as quantum dots (QDs), are promising imaging probes with characteristic optical properties: tunable bandgap from visible to infrared, narrow and symmetric emission features, broad absorption, high quantum yield (QY), excellent photostability, and a large two-photon absorption cross section. However, unlike other imaging probes, the surface ligands determine the solubility, stability, quantum yield (QY), biocompatibility, and derivatizability of QDs. Therefore, to use QDs for in vitro and in vivo imaging, QD ligands need to be elaborately engineered for each experiment. Single cell labeling in vivo requires extremely strict criteria for the QD conjugates to be used such as minimal nonspecific cell/serum binding, maintenance of high QY in complex in vivo environments, and compact size. The focus of this thesis is the synthesis of high quality QD conjugates that can be used for single molecule imaging in vivo and in vivo imaging studies that demonstrate the broad and powerful applicability of our new methods. We incorporated novel conjugation methods employing highly strained cycloolefins and a serum stable tetrazine derivative into newly developed polymeric imidazole ligands (PILs) to efficiently couple biomolecules on QDs. Unlike traditional conjugation methods, tetrazine-norbornene cycloaddition benefits from the non-interacting properties of the functional groups to the QD surface, and yields the high conjugation efficiencies on QDs. In addition, the rapid kinetics, absence of catalyst, and bio-orthogonality of the cycloaddition allowed us to achieve in situ conjugation of the norbornene-bearing QDs to tetrazine-bearing epidermal growth factor (EGF) proteins on the HeLa cell surface. On the in vivo front, we accomplished single endogenous cell imaging in live mice. The ability to target single cells using multiple biomarkers and track them for the extended periods of time allowed us to study the microenvironment of the endogenous hematopoietic stem cells (HSCs), which was not possible using conventional techniques engaging dye conjugated antibodies. Lastly, a new class of QD ligands containing betaine moieties was developed to reduce the size of QD conjugates, which we expect will be greatly beneficial for in vitro and in vivo targeting in dense environments. We successfully demonstrated functionalization of the sulfonate betaine poly imidazole ligands (SBPILs) with biomolecules, and the biocompatibility of SBPIL QDs both in vitro and in vivo.

Download or read book Quantum Dots written by Adriana Fontes and published by Humana. This book was released on 2021-04-18 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition provides revised and expanded protocols of consolidated approaches as well as new trends in the field. Chapters guide readers through new approaches to optimize Quantum Dots’ (QD) properties, to evaluate their quantum yields, important features about preparative processes and characterizations of QDs, methods related to QDs for live cell applications, and the versatility of QDs in the bioanalytical and biosensing field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Download or read book Quantum Dots in Bioanalytical Chemistry and Medicine written by Zahra Ramezani and published by Royal Society of Chemistry. This book was released on 2023-08-11 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces the various types of quantum dots to the reader and provides enough information in the text and the references to encourage a new quantum dot user to get started.