Download or read book Quantitative Analysis Using Chromatographic Techniques written by Katz E. and published by . This book was released on 1987 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Quantitative Analysis Using Chromatographic Techniques written by Elena Katz and published by . This book was released on 1987-02-27 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the only book that covers only quantitative techniques of all chromatography methods. Contributors, experts in the field, present general concepts applicable to all types of analyses performed by chromatographic techniques, and treat in detail principles of quantitative detection and sources of error in data processing procedures. Chapters cover liquid chromatography, gas chromatography, thin-layer chromatography, detection methods, detector performance, data handling procedures, and automation procedures. Included is discussion of chromatographic applications in physical chemistry and in pharmaceutical analysis.
Download or read book Bioanalysis of Pharmaceuticals written by Steen Honoré Hansen and published by John Wiley & Sons. This book was released on 2015-07-20 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioanalysis of Pharmaceuticals: Sample Preparation, Separation Techniques and Mass Spectrometry is the first student textbook on the separation science and mass spectrometry of pharmaceuticals present in biological fluids with an educational presentation of the principles, concepts and applications. It discusses the chemical structures and properties of low- and high-molecular drug substances; the different types of biological samples and fluids that are used; how to prepare the samples by extraction, and how to perform the appropriate analytical measurements by chromatographic and mass spectrometric methods. Bioanalysis of Pharmaceuticals: Sample Preparation, Separation Techniques and Mass Spectrometry: Is an introductory student textbook discussing the different principles and concepts clearly and comprehensively, with many relevant and educational examples Focuses on substances that are administered as human drugs, including low-molecular drug substances, peptides, and proteins Presents both the basic principles that are regularly taught in universities, along with the practical use of bioanalysis as carried out by researchers in the pharmaceutical industry and in hospital laboratories Is aimed at undergraduate students, scientists, technicians and researchers in industry working in the areas of pharmaceutical analyses, biopharmaceutical analyses, biological and life sciences The book includes multiple examples to illustrate the theory and application, with many practical aspects including calculations, thus helping the student to learn how to convert the data recorded by instruments into the real concentration of the drug substances within the biological sample.
Download or read book Quantitative Chromatographic Analysis written by Thomas Beesley and published by CRC Press. This book was released on 2000-12-06 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highlights critical factors involved in successful chromatographic analysis. Details analytical procedures; outlining sample preparation, collection, transportation, and storage. Provides step-by-step guidelines for producing analytical reports.
Download or read book Quantitative In Silico Chromatography written by Toshihiko Hanai and published by Royal Society of Chemistry. This book was released on 2014-05-07 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: The coupling of mass spectrometry or nuclear magnetic resonance to chromatography has broadened the possibilities for determining organic reaction mechanisms. And while many results have been published reporting these, even more can be achieved through modern computational methods. Combining computational and theoretical techniques with advanced chromatographic methods offers a powerful tool for quantitatively determining molecular interactions . This book presents the possibilities for characterising biological applications by combining analytical and computational chemistries. Written by the author of “HPLC: A Practical Guide” (RSC, 1999), the book examines not only the behaviour of biological reactions per se, but also describes the behaviour of biological molecules in chromatography systems. Various software packages are reviewed, and most computations can be performed on a standard PC using accessible software. Consideration is given to a variety of chromatographic techniques and strategies for high-sensitivity detection are presented. The first book of its kind, it will inspire readers to explore the possibilities of combining these techniques in their own work, whether at an industrial or academic level.
Download or read book Data Analysis and Signal Processing in Chromatography written by A. Felinger and published by Elsevier. This book was released on 1998-05-19 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives an overview of the numerical data analysis and signal treatment techniques that are used in chromatography and related separation techniques. Emphasis is given to the description of the symmetrical and asymmetrical chromatographic peak shape models. Both theoretical and empirical models are discussed. The fundamentals of data acquisition, types and effect of baseline noise, and methods of improving the signal-to-noise ratio (either in time or in frequency and wavelet domain) are thoroughly discussed. Resolution enhancement techniques, such as curve fitting, deconvolution by Fourier and wavelet transforms, iterative deconvolution, Kalman filtering and multivariate methods of curve resolution are all discussed with several chromatographic examples. Quantitative analysis by peak area of peak height measurement, the precision and accuracy of the quantitation of stand-alone or overlapping and symmetrical or asymmetrical peaks are treated. In a separate chapter, guidelines are given for the use of transform techniques for the analysis of chromatograms. A statistical description of peak overlap is given in the final chapters. Since the concept of resolution has to be reconsidered when one separates complex mixtures, the problem of resolution and overlap is quantitatively discussed by means of statistical methods, and by using Fourier analysis of the complex chromatogram. Features of this book • The ultimate source of numerical techniques to enhance chromatographic data • Gives a detailed description of signal and resolution enhancement techniques in a manner applicable for enhancing not only chromatography, but also spectroscopic and other analytical signals • The first book with a thorough overview of the statistics of peak overlap. This is the first volume to encompass both the simple and more sophisticated methods for the numerical treatment of chromatograms. It is, therefore, the fundamental resource of numerical analysis methods for every analyst.
Download or read book Quantitation of Amino Acids and Amines by Chromatography written by Ibolya Molnár-Perl and published by Elsevier. This book was released on 2005-06-27 with total page 669 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitation of Amino Acids and Amines by Chromatography: Methods and Protocols is intended to serve as a ready-to-use guide for the identification and quantification of amino acids and amines in various matrices, providing an overview on the theory and protocol of available methods. It presents chromatograms with exact elution programs enabling visual analysis and compares the advantages-disadvantages of various chromatographic techniques. In accordance with the chronological order of the development of chromatographic methods, different techniques are discussed: The possibilities of gas chromatography (GC), followed by those of the high performance liquid chromatography (HPLC) and the most recent techniques capillary electrophoresis (CE), capillary, electrochromatography (CEC). The characteristics of the given chromatographic procedure, relating to the topic in question, are classified according to the preliminary preparation/derivatization process(es), which means the simple methods, suitable for the analysis of the selected compound(s) in natural form, are followed by various derivatization proposals. Detailed protocols provide the reader with guidance in beginning tasks and on how to improve current methods. This book appeals to a wide audience and is recommended for those looking towards the wider reaches of identification and quantification of amino acids and amines. * Provides a systematic, and comprehensive summary of chromatographic techniques and derivatization processes * Compares advantages/disadvantages of various chromatographic techniques * Readers can undertake practical tasks using detailed protocols given in the book
Download or read book Modern Sample Preparation for Chromatography written by and published by Elsevier. This book was released on 2014-10-18 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sample preparation is applied to make real world samples amenable for chromatographic analysis, or to improve the results of this type of analysis. A wide variety of procedures are applied for this purpose, and their description is the main goal of the present book. The principles of these procedures are explained, discussing their advantages and disadvantages, and their applicability to different types of samples as well as their fit for different types of chromatographic analysis. This provides a guide for choosing the appropriate sample preparation for a given analysis. The book also contains numerous literature references and examples of sample preparation for different matrices. The material is presented in three parts, one discussing physical methods used in sample preparation such as filtration, distillation, solvent extraction, solid phase extraction, electro-separations. Presents in a systematic way numerous techniques applied for sample preparation for chromatographic analysis Provides an up to date source of information regarding the progress made in sample preparation for chromatography Describes examples for specific type of matrices, providing a guide for choosing the appropriate sample preparation method for a given analysis
Download or read book Comprehensive Two dimensional Chromatographic Techniques for Ultra trace Quantitative Analysis of Chlorinated Dioxins in South Africa written by Betty-Jayne de Vos and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) are toxic environmental pollutants formed as by-products of industrial and thermal processes. They are chlorinated compounds that have similar structures and chemical properties that were included in the original United Nations Environment Programme’s ‘dirty dozen’ and now form part of the Stockholm Convention (SC) on Persistent Organic Pollutants (POPs). As a signatory to the Stockholm Convention, South Africa has the obligation to undertake appropriate research, monitoring, and cooperation pertaining to POPs, and more particularly, PCDD/Fs. Currently there is no established PCDD/F laboratory in South Africa capable of these demanding measurements and alternative approaches must be considered that are more affordable, more robust and more user friendly for a developing economy. PCDD/Fs are highly toxic, causing a myriad of negative human health effects such as chloracne, carcinogenicity, hepatotoxicity, teratogenicity, endocrine disruption and alterations in neural development. The toxicity of PCDD/Fs is mediated through the aryl hydrocarbon receptor (AhR). PCDD/Fs bind to the AhR and elicit an AhR-mediated biochemical and toxic response. The in-depth information available on the mechanism of toxicity also allows for PCDD/Fs to be analysed using the AhR receptor mediated response in genetically modified cell lines. This led to the development of a bio-analytical screening technique using in vitro H4IIE-luc reporter gene bio-assay for AhR active compounds for the initial screening of PCDD/Fs in soil and sediment. The intension in this study was to develop an integrated approach to the analysis of PCDD/Fs in the South African environment, considering the associated cost as well as the available instrumentation, expertise and relevance within a developing economy. Historically, the quantitative confirmatory analysis of these compounds has been achieved by targeted analysis using gas chromatography coupled with high-resolution magnetic sector mass spectrometry instruments (GC-HRMS), the accepted benchmark technology used for determining the level of trace organic environmental contaminants such as PCDD/Fs. However, these methods are time consuming and expensive. Advances in technology have led to comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (GC×GC-TOFMS) methodology that can be used for the analysis of PCDD/Fs in samples with different matrices. This approach is well suited for application in developing economies where access to GC-HRMS and highly skilled personnel is limited. This thesis describes the bio-analytical technique and the method development and analysis of the seventeen toxic PCDD/F congeners using GC×GC-TOFMS methodology. The technique provides the selectivity (added peak capacity of GC×GC) and the sensitivity (focusing effect of the modulator) needed to meet the requirements as mandated for analysis in US EPA Method 1613B. Extracted samples analysed on a GC-HRMS instrument, were re-analyzed using the low-resolution GC×GC-TOFMS instrument and the results confirmed using a high resolution TOF mass spectrometer (HRT). The quantitative results obtained compare well with those obtained using GC-HRMS. Because GC×GC-TOFMS is not a target compound analytical technique (as is GC-HRMS), it is possible to obtain information on numerous other classes of organic pollutants present in the samples in one analytical run, although this information can be sample clean-up dependant. Preliminary validation of the GC×GC-TOFMS method is investigated using a certified reference material and real South African soil samples. The South African soil samples studied showed extremely high levels of PAHs, aliphatic hydrocarbons and sulphur. The organic content and matrix interference of South African soil samples (and the NIST standard reference material sediment; SRM 1944) provided significant challenges for the validation study. This study has shown that GC×GC-TOFMS provides a quick, convenient screen for numerous pollutant classes which may be present in environmental samples. Retrospective data mining of archived data (extraction dependant) is possible and has provided key information on other chlorinated and brominated contaminants present in South African waste, soil and sediment samples. NMISA now has a viable GC×GC-TOFMS dioxin analytical method for low level (ultra-trace) quantitative screening of chlorinated compounds that can be offered to South African analytical laboratories for routine dioxin analysis. The work is relevant scientifically and is a definitive contribution to the growing compilation of GC×GC methodology, providing efficient methods for this demanding environmental application.
Download or read book Quantitative Thin Layer Chromatography written by Bernd Spangenberg and published by Springer. This book was released on 2014-11-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological detection methods. Chapters are organized in a modular fashion facilitating the easy location of information about individual procedural steps.
Download or read book Trace Quantitative Analysis by Mass Spectrometry written by Robert K. Boyd and published by John Wiley & Sons. This book was released on 2011-08-24 with total page 1179 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a serious introduction to the subject of mass spectrometry, providing the reader with the tools and information to be well prepared to perform such demanding work in a real-life laboratory. This essential tool bridges several subjects and many disciplines including pharmaceutical, environmental and biomedical analysis that are utilizing mass spectrometry: Covers all aspects of the use of mass spectrometry for quantitation purposes Written in textbook style to facilitate understanding of this topic Presents fundamentals and real-world examples in a ‘learning-though-doing’ style
Download or read book Selection of the HPLC Method in Chemical Analysis written by Serban C. Moldoveanu and published by Elsevier. This book was released on 2016-11-01 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection, development, and validation. High-performance liquid chromatography (HPLC) is the most common analytical technique currently practiced in chemistry. However, the process of finding the appropriate information for a particular analytical project requires significant effort and pre-existent knowledge in the field. Further, sorting through the wealth of published data and literature takes both time and effort away from the critical aspects of HPLC method selection. For the first time, a systematic approach for sorting through the available information and reviewing critically the up-to-date progress in HPLC for selecting a specific analysis is available in a single book. Selection of the HPLC Method in Chemical Analysis is an inclusive go-to reference for HPLC method selection, development, and validation. Addresses the various aspects of practice and instrumentation needed to obtain reliable HPLC analysis results Leads researchers to the best choice of an HPLC method from the overabundance of information existent in the field Provides criteria for HPLC method selection, development, and validation Authored by world-renowned HPLC experts who have more than 60 years of combined experience in the field
Download or read book Quantitative Column Liquid Chromatography written by S.T. Balke and published by Elsevier. This book was released on 2000-04-01 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitative Column Liquid Chromatography
Download or read book Chromatographic Analysis of the Environment written by Leo M.L. Nollet and published by CRC Press. This book was released on 2017-03-03 with total page 650 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed handbook covers different chromatographic analysis techniques and chromatographic data for compounds found in air, water, and soil, and sludge. The new edition outlines developments relevant to environmental analysis, especially when using chromatographic mass spectrometric techniques. It addresses new issues, new lines of discussion, and new findings, and develops in greater detail the aspects related to chromatographic analysis in the environment. It also includes different analytical methodologies, addresses instrumental aspects, and outlines conclusions and perspectives for the future.
Download or read book Chromatographic Methods written by A. Braithwaite and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 573 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a unified and balanced introduction to the general theory of chromatography, followed by a detailed treatment of the principles and practice of all the major techniques currently employed in the industrial and academic sectors. It is written as a broad introduction to the subject for mid to advanced undergraduates in chemistry, pharmacy, biochemistry, and is suitable for students following the now quite numerous Masters degrees in instrumental analysis. The book has been updated to incorporate advances of the last ten years, and it contains around 50% new or revised material.
Download or read book Advances in Chemical Analysis Procedures Part II written by Marcello Locatelli and published by MDPI. This book was released on 2021-01-20 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the field of Analytical Chemistry and, in particular, whenever a quali-quantitative analysis is required, until a few years ago, reference was made exclusively to instrumental methods (more or less hyphenated) which, once validated, were able to provide the answers to the questions present, even if only in a limited way to analytical targets. Nowadays, the landscape has become considerably complicated (natural adulterants, assessment of geographical origin, sophistication, need for non-destructive analysis, search for often unknown compounds), and new procedures for processing data have greatly increased the potential of analyses that are conducted (even routinely) in the laboratory. In this scenario, chemometrics is master, able to manage and process a huge amount of information based both on data relating only to the analytes of interest, but also by applying “general” procedures to process raw untargeted analysis data. It is within this strand of analysis that many of the works reported in this Special Issue fall. In the succession of works in this printed version, the criterion that guided us was to highlight how—starting exclusively from chromatographic techniques (HPLC and GC) with conventional detectors and moving to exclusively spectroscopic techniques (MS, FT-IR and Raman)—it is possible arrive at extremely powerful coupled techniques and procedures (HPLC and FT-IR) able to meet research needs. Finally, at the end of the printed volume, there are two reviews that surveying the state of the art regarding the assessment of authenticity through qualitative analyses and the application of chemometrics in the pharmaceutical field in the study of forced drug degradation products. From the succession of works (and, above all, from the various application fields) it can immediately be seen how the application of chemometrics and its procedures to both raw and processed data is a powerful means of obtaining robust, reproducible, and predictive information. In this manner, it is possible to create models able to explain and respond to the original problem in a much more detailed way. , and Honghe through Fourier transform mid infrared (FT-MIR) spectra combined with partial least squares discriminant analysis (PLS-DA), random forest (RF), and hierarchical cluster analysis (HCA) methods. Melucci and collaborators apply chemometric approaches to non-destructive analysis of ATR-FT-IR for the determination of biosilica content. This value was directly evaluated in sediment samples, without any chemical alteration, using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, and the quantification was performed by combining the multivariate standard addition method (MSAM) with the net analyte signal (NAS) procedure to solve the strong matrix effect of sediment samples. Still in the food and food supplements field, Anguebes-Franseschi and collaborators report an article where 10 chemometric models based on Raman spectroscopy were applied to predict the physicochemical properties of honey produced in the state of Campeche, Mexico.
Download or read book Chemometrics in Chromatography written by Łukasz Komsta and published by CRC Press. This book was released on 2018-02-02 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemometrics uses advanced mathematical and statistical algorithms to provide maximum chemical information by analyzing chemical data, and obtain knowledge of chemical systems. Chemometrics significantly extends the possibilities of chromatography and with the technological advances of the personal computer and continuous development of open-source software, many laboratories are interested in incorporating chemometrics into their chromatographic methods. This book is an up-to-date reference that presents the most important information about each area of chemometrics used in chromatography, demonstrating its effective use when applied to a chromatographic separation.
